• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권10호
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• pp.4844-4864
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• 2017

Interference-aware routing protocol design for underwater wireless sensor networks (UWSNs) is one of the key strategies in reducing packet loss in the highly hostile underwater environment. The reduced interference causes efficient utilization of the limited battery power of the sensor nodes that, in consequence, prolongs the entire network lifetime. In this paper, we propose an energy-efficient interference-aware routing (EEIAR) protocol for UWSNs. A sender node selects the best relay node in its neighborhood with the lowest depth and the least number of neighbors. Combination of the two routing metrics ensures that data packets are forwarded along the least interference paths to reach the final destination. The proposed work is unique in that it does not require the full dimensional localization information of sensor nodes and the network total depth is segmented to identify source, relay and neighbor nodes. Simulation results reveal better performance of the scheme than the counterparts DBR and EEDBR techniques in terms of energy efficiency, packet delivery ratio and end-to-end delay.

• 한국콘텐츠학회논문지
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• 제16권8호
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• pp.202-208
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• 2016

시각장애인의 활동범위가 늘어나면서 복잡하고 대형화된 건물들 속에서 목적지까지 안전하게 찾아가기란 쉽지 않다. 시각장애인을 위해 GPS 신호나 음성 알림 정보, 점자 유도 블록, 음향 신호기 등을 활용한 안내 시스템이 있지만 이는 대부분 실외 안내 시스템으로 실내에서는 적합하지 않다. 이에 본 논문에서는 보편화된 스마트 폰을 이용하여 시각장애인에게 해당 목적지에 대한 방향, 거리, 높이, 장애물 등의 목적지까지의 다양한 정보를 음성기술인 TTS(Text to Speech)와 촉각기술인 햅틱(Haptic) 그리고 블루투스 4.0기반의 근거리 무선통신 기술인 비콘을 이용하여 사용자에게 알려 줄 수 있는 실내 위치 추정 및 안내 시스템을 제안한다. 제한된 시스템의 실험 결과에서 사용자는 목적지까지의 최적 경로를 검색하여 TTS와 Haptic 기술을 이용해 안전하고 정확하게 안내받을 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.712-717
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• 2010

Cytochrome P450 enzymes (P450s) are involved in the synthesis of a wide variety of valuable products and in the degradation of numerous toxic compounds. The P450 BM3 (CYP102A1) from Bacillus megaterium was the first P450 discovered to be fused to its redox partner, a mammalian-like diflavin reductase. Here, we report the development of a whole-cell biocatalyst using ice-nucleation protein (Inp) from Pseudomonas syringae to display a hemeand diflavin-containing oxidoreductase, P450 BM3 (a single, 119-kDa polypeptide with domains of both an oxygenase and a reductase) on the surface of Escherichia coli. The surface localization and functionality of the fusion protein containing P450 BM3 were verified by flow cytometry and measurement of enzymatic activities. The results of this study comprise the first report of microbial cell-surface display of a heme- and diflavin-containing enzyme. This system should allow us to select and develop oxidoreductases containing heme and/or flavins into practically useful whole-cell biocatalysts for extensive biotechnological applications, including selective synthesis of new chemicals and pharmaceuticals, bioconversion, bioremediation, live vaccine development, and biochip development.

• Advances in Energy Research
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• 제5권2호
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• pp.179-205
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• 2017

This research presents an in-depth analysis of location planning of the solar-hydrogen power plants for electricity production in different cities situated in Kerman province of Iran. Ten cities were analyzed in order to select the most suitable location for the construction of a solar-hydrogen power plant utilizing photovoltaic panels. Data envelopment analysis (DEA) methodology was applied to prioritize cities for installing the solar-hydrogen power plant so that one candidate location was selected for each city. Different criteria including population, distance to main road, flood risk, wind speed, sunshine hours, air temperature, humidity, horizontal solar irradiation, dust, and land costare used for the analysis. From the analysis, it is found that among the candidates' cities, the site of Lalezar is ranked as the first priority for the solar-hydrogen system development. A measure of validity is obtained when results of the DEA method are compared with the results of the technique for ordering preference by similarity to ideal solution (TOPSIS). Applying TOPSIS model, it was found that city of Lalezar ranked first, and Rafsanjan gained last priority for installing the solar-hydrogen power plants. Cities of Baft, Sirjan, Kerman, Shahrbabak, Kahnouj, Shahdad, Bam, and Jiroft ranked second to ninth, respectively. The validity of the DEA model is compared with the results of TOPSIS and it is demonstrated that the two methods produced similar results. The solar-hydrogen power plant is considered for installation in the city of Lalezar. It is demonstrated that installation of the proposed solar-hydrogen system in Lalezar can lead to yearly yield of 129 ton-H2 which covers 4.3% of total annual energy demands of the city.

• Molecules and Cells
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• 제37권2호
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• pp.126-132
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• 2014

As a tumor suppressor homologue during mitosis, Chk2 is involved in replication checkpoints, DNA repair, and cell cycle arrest, although its functions during mouse oocyte meiosis and early embryo development remain uncertain. We investigated the functions of Chk2 during mouse oocyte maturation and early embryo development. Chk2 exhibited a dynamic localization pattern; Chk2 expression was restricted to germinal vesicles at the germinal vesicle (GV) stage, was associated with centromeres at pro-metaphase I (Pro-MI), and localized to spindle poles at metaphase I (MI). Disrupting Chk2 activity resulted in cell cycle progression defects. First, inhibitor-treated oocytes were arrested at the GV stage and failed to undergo germinal vesicle breakdown (GVBD); this could be rescued after Chk2 inhibition release. Second, Chk2 inhibition after oocyte GVBD caused MI arrest. Third, the first cleavage of early embryo development was disrupted by Chk2 inhibition. Additionally, in inhibitor-treated oocytes, checkpoint protein Bub3 expression was consistently localized at centromeres at the MI stage, which indicated that the spindle assembly checkpoint (SAC) was activated. Moreover, disrupting Chk2 activity in oocytes caused severe chromosome misalignments and spindle disruption. In inhibitor-treated oocytes, centrosome protein ${\gamma}$-tubulin and Polo-like kinase 1 (Plk1) were dissociated from spindle poles. These results indicated that Chk2 regulated cell cycle progression and spindle assembly during mouse oocyte maturation and early embryo development.

• BMB Reports
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• 제41권6호
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• pp.415-434
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• 2008

Phosphoinositide-specific phospholipase C is an effector molecule in the signal transduction process. It generates two second messengers, inositol-1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Currently, thirteen mammal PLC isozymes have been identified, and they are divided into six groups: PLC-$\beta$, -$\gamma$, -$\delta$, -$\varepsilon$, -$\zeta$ and -$\eta$. Sequence analysis studies demonstrated that each isozyme has more than one alternative splicing variant. PLC isozymes contain the X and Y domains that are responsible for catalytic activity. Several other domains including the PH domain, the C2 domain and EF hand motifs are involved in various biological functions of PLC isozymes as signaling proteins. The distribution of PLC isozymes is tissue and organ specific. Recent studies on isolated cells and knockout mice depleted of PLC isozymes have revealed their distinct phenotypes. Given the specificity in distribution and cellular localization, it is clear that each PLC isozyme bears a unique function in the modulation of physiological responses. In this review, we discuss the structural organization, enzymatic properties and molecular diversity of PLC splicing variants and study functional and physiological roles of each isozyme.

• Macromolecular Research
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• 제16권1호
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• pp.15-20
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• 2008

The enhanced permeability and retention (EPR) effect is used extensively for the passive targeting of many macromolecular drugs for tumors. Indeed, the EPR concept has been a gold standard in polymeric anticancer drug delivery systems. This study investigated the tumoral distribution of self-assembled nanoparticles based on the EPR effect using fluorescein and radio-labeled nanoparticles. Self-assembled nanoparticles were prepared from amphiphilic chitosan derivatives, and their tissue distribution was examined in tumor-bearing mice. The size of the nanoparticles was controlled to be 330 run, which is a size suited for opening between the defective endothelial cells in tumors. The long-circulating polymer nanoparticles were allowed to gradually accumulate in the tumors for 11 days. The amount of nanoparticles accumulated in the tumors was remarkably augmented from 3.4%ID/g tissue at 1 day to 25.9%ID/g tissue at 11 days after i.v. administration. The self-assembled nanoparticles were sustained at a high level throughout the 14 day experimental period, indicating their long systemic retention in the blood circulation. The ${\gamma}$-images provided clear evidence of selective tumor localization of the $^{131}I$-labeled nanoparticles. Confocal microscopy revealed the fluorescein-labeled nanoparticles to be preferentially localized in the perivascular regions, suggesting their extravasation to the tumors through the hyperpermeable angiogenic tumor vasculature. This highly selective tumoral accumulation of nanoparticles was attributed to the leakiness of the blood vessels in the tumors and their long residence time in the blood circulation.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.2
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• pp.83-88
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• 2006

Many applications in the area of location-based services and personal navigation require nowadays the location determination of a user not only in outdoor environment but also indoor. To locate a person or object in a building, systems that use either infrared, ultrasonic or radio signals, and visible light for optical tracking have been developed. The use of WiFi for location determination has the advantage that no transmitters or receivers have to be installed in the building like in the case of infrared and ultrasonic based location systems. WiFi positioning technology adopts IEEE802.11x standard, by observing the radio signals from access points installed inside a building. These access points can be found nowadays in our daily environment, e.g. in many office buildings, public spaces and in urban areas. The principle of operation of location determination using WiFi signals is based on the measurement of the signal strengths to the surrounding available access points at a mobile terminal (e.g. PDA, notebook PC). An estimate of the location of the terminal is then obtained on the basis of these measurements and a signal propagation model inside the building. The signal propagation model can be obtained using simulations or with prior calibration measurements at known locations in an offline phase. The most common location determination approach is based on signal propagation patterns, namely WiFi fingerprinting. In this paper the underlying technology is briefly reviewed followed by an investigation of two WiFi positioning systems. Testing of the system is performed in two localization test beds, one at the Vienna University of Technology and the second at the Hong Kong Polytechnic University. First test showed that the trajectory of a moving user could be obtained with a standard deviation of about ${\pm}$ 3 m.

• Journal of Ginseng Research
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• 제45권6호
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• pp.734-743
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• 2021

Background: The underlying mechanisms of the potential tumor-suppressive effects of ginsenoside Rh2 are complex. N6-methyladenosine (m6A) RNA methylation is usually dysregulated in cancer. This study explored the regulatory effect of ginsenoside Rh2 on m6A RNA methylation in cancer. Methods: m6A RNA quantification and gene-specific m6A RIP-qPCR assays were applied to assess total and gene-specific m6A RNA levels. Co-immunoprecipitation, fractionation western blotting, and immunofluorescence staining were performed to detect protein interactions and distribution. QRT-PCR, dual-luciferase, and ChIP-qPCR assays were conducted to check the transcriptional regulation. Results: Ginsenoside Rh2 reduces m6A RNA methylation and KIF26B expression in a dose-dependent manner in some cancers. KIF26B interacts with ZC3H13 and CBLL1 in the cytoplasm of cancer cells and enhances their nuclear distribution. KIF26B inhibition reduces m6A RNA methylation level in cancer cells. SRF bound to the KIF26B promoter and activated its transcription. SRF mRNA m6A abundance significantly decreased upon KIF26B silencing. SRF knockdown suppressed cancer cell proliferation and growth both in vitro and in vivo, the effect of which was partly rescued by KIF26B overexpression. Conclusion: ginsenoside Rh2 reduces m6A RNA methylation via downregulating KIF26B expression in some cancer cells. KIF26B elevates m6A RNA methylation via enhancing ZC3H13/CBLL1 nuclear localization. KIF26B-SRF forms a positive feedback loop facilitating tumor growth.

• 항공우주시스템공학회지
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• 제13권1호
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• pp.54-61
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• 2019

3D프린팅은 '12년 세계경제포럼에서 떠오르는 10대 기술 중 2번째로 선정이 되었고 기존 산업의 패러다임을 변화시켜 제조업 혁신과 신 시장을 창출할 핵심기술로 대두되었다. 미국 등 주요 선도 국가들은 3D 프린팅을 국방 분야에 적극 확대해 나가고 있으며, 우리나라 역시 3D 프린팅의 군사적 활용을 위해 국방 수리부품 생산기술 확보 및 국방전문인력 양성을 추진하고 있다. 이에 본 논문은 기존의 무기체계 개발단계, 양산단계, 운영유지단계의 부품 국산화 개발관리 절차를 활용하여 향후 신뢰성 있는 3D 프린팅 수리부품을 효율적으로 작전현장에 적용하기 위한 방안을 제시하였다.