• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 추계학술대회 논문집
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• pp.518-523
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• 2008

경부고속열차(KTX) 차량컴퓨터제어장치(OBCS : On Board Computer System)는 네트워크 통신을 이용하여 차량내 각 종 전장품의 동작상태 감시와 운행명령 및 열차제어, 차량의 유지보수, 승무원의 운전업무를 지원하는 장치로써 KTX의 핵심장치이다. 이 장치는 TGV 제작사양과 프랑스의 고속열차 운영방식에 종속되어 특화된 시스템으로 제작되었으므로 실질적인 기술이전이 어려운 상태이다. 그렇기때문에 KTX 국내도입 후 운영상 요구되는 기능개선 및 자체적인 유지보수, 각 종 전장품(추진제어장치, 보조전원장치, 출입문제어장치 등)의 국산화 교체시 대부분의 인터페이스 신호가 차량컴퓨터제어장치(OBCS)와 연계되어 있어 많은 어려움이 수반되고 있다. 따라서 본 연구에서는 KTX 차량컴퓨터제어장치의 기능분석 및 주변장치(기관사 콘솔, 여객정보시스템, 고장현시장치, 각종 전장품 등)와의 상호 인터페이스 정보분석을 통해 KTX-OBCS 국산화 개발을 함으로써 KTX 운영상 요구되는 기능개선 및 유지보수 효율화에 기여하고, 더 나아가 고속열차제어 및 전장품 분야에서 선진 외국기술의 종속을 탈피하여 기술자립화를 이루고자 한다.

• Genes and Genomics
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• 제40권12호
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• pp.1259-1267
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• 2018

Litchi (Litchi chinensis Sonn.) is an important subtropical fruit crop with high commercial value due to its high nutritional values and favorable tastes. However, irregular bearing attributed to unstable flowering is a major ongoing problem for litchi producers. Previous studies indicate that low-temperature is a key factor in litchi floral induction. In order to reveal the genetic and molecular mechanisms underlying the reproductive process in litchi, we had analyzed the transcriptome of buds before and after low-temperature induction using RNA-seq technology. A key flower bud differentiation associated gene, a homologue of FLORICAULA/LEAFY, was identified and named LcLFY (GenBank Accession No. KF008435). The cDNA sequence of LcLFY encodes a putative protein of 388 amino acids. To gain insight into the role of LcLFY, the temporal expression level of this gene was measured by real-time RT-PCR. LcLFY was highly expressed in flower buds and its expression correlated with the floral developmental stage. Heterologous expression of LcLFY in transgenic tobacco plants induced precocious flowering. Meantime, we investigated the sub-cellular localization of LcLFY. The LcLFY-Green fluorescent protein (GFP) fusion protein was found in the nucleus. The results suggest that LcLFY plays a pivotal role as a transcription factor in controlling the transition to flowering and in the development of floral organs in litchi.

• 한국콘텐츠학회논문지
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• 제19권3호
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• pp.72-79
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• 2019

자율 주행은 이동 로봇의 핵심적 기술로써, 측정된 센서 정보를 토대로 불확실한 위치 정보를 이용한 지도 작성 및 수정 기능과, 불확실한 지도 및 센서 정보를 이용한 로봇의 위치 인식 기능으로 구성된다. 자율주행은 이러한 주행 위치의 불확실성에 기반한 확률론적 방법론과 함께 주행 시 장애물의 감지 및 회피 경로의 생성, 반복적 주행 패턴에 따른 경로 관리 기능이 필수적 요소이다. 거리 기반의 스캐너를 통해 관측된 센서 입력은, 지도 구성에 사용된 벽과 같은 정적 물체와 주행 시의 사람처럼 움직이는 동적 물체와의 구별이 필요하기 ?문에 장애물 감지에 어려움이 있다. 본 논문에서는, 이러한 자율 주행 환경에서 기존의 정적, 동적 개체의 판별 방식과 비교하여, 장애물 회피를 위한 저해상도 격자 공간의 생성 및 강화학습을 이용한 경로 생성을 다루고자 한다. 최종적으로 실험을 통해 제안된 방법론의 실효성을 검증하고자 한다.

• Healthcare Informatics Research
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• 제24권4호
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• pp.394-401
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• 2018

Objectives: Augmented reality (AR) technology has become rapidly available and is suitable for various medical applications since it can provide effective visualization of intricate anatomical structures inside the human body. This paper describes the procedure to develop an AR app with Unity3D and Vuforia software development kit and publish it to a smartphone for the localization of critical tissues or organs that cannot be seen easily by the naked eye during surgery. Methods: In this study, Vuforia version 6.5 integrated with the Unity Editor was installed on a desktop computer and configured to develop the Android AR app for the visualization of internal organs. Three-dimensional segmented human organs were extracted from a computerized tomography file using Seg3D software, and overlaid on a target body surface through the developed app with an artificial marker. Results: To aid beginners in using the AR technology for medical applications, a 3D model of the thyroid and surrounding structures was created from a thyroid cancer patient's DICOM file, and was visualized on the neck of a medical training mannequin through the developed AR app. The individual organs, including the thyroid, trachea, carotid artery, jugular vein, and esophagus were localized by the surgeon's Android smartphone. Conclusions: Vuforia software can help even researchers, students, or surgeons who do not possess computer vision expertise to easily develop an AR app in a user-friendly manner and use it to visualize and localize critical internal organs without incision. It could allow AR technology to be extensively utilized for various medical applications.

• 한국산학기술학회논문지
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• 제19권11호
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• pp.191-204
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• 2018

다양성과 창의성의 시대에 지방이 스스로 생각하고 실행하는 지역발전 전략이 강조되고 있으며, 지역의 유산 재활용을 통한 지역차원의 재생효과와 지역자산으로의 가치에 대한 인식이 상승하고 있다. 상당기간 낙후 소외되어 온 지역의 시설과 공간을 기회자산으로 전환시킬 수 있는 도시재생방안 모색은 매우 의미 있는 일이라 하겠다. 본 연구는 산업유산의 지역자원화를 통해 내생적인 발전체계 구축하여 지역의 자립적인 발전을 추구할 가능성 모색에 있다. 사례분석과 계획적용 등을 통해 생활밀착형 문화공간이 대형, 전문예술공간보다 지역주민의 삶에 더 큰 영향을 준다는 점을 확인할 수 있었다. 지역의 비교우위보다는 지역자산의 경쟁우위 전략이 더욱 중요하며, 장래의 도심재생에서 한층 더 관심을 두어야 하는 요소는 도시의 역사와 전통, 철학 등 무형적인 가치라는 사실을 인지하는 것이 필요하고, 사람이 중심이 되어 예술적인 생태성과 전통의 복원, 공동체성 창출, 미학성 추구 등을 통해 문화정체성을 가진 장소로 창조되는 것이 진정한 도시재생이 된다는 점이다.

• 대한수의학회지
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• 제59권1호
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• pp.17-24
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• 2019

Animal models of osteoarthritis (OA) have played a key role in understanding the etiology of OA and in the development of new therapeutic strategies. Although pigs have an advantage as an animal disease model due to their similarity to humans, there are few studies on the induction of OA in minipigs. Therefore, this study aimed to characterize disease progression of OA in total medial meniscectomy (TMM)-operated skeletally mature minipigs, up to day 180 postoperatively. There were no significant alterations in vital signs or hematological indices throughout the observation period. However, clinical manifestations of OA in the medial femoral condyles of TMM-operated minipigs were progressive, depending on postoperative duration, with respect to osteophytes formation and roughened surfaces on radiological observation, cartilage erosion under macroscopic examination, and severe cartilage defects including fibrillation, vertical fissures, and cartilage denuding on histopathological observation, with the highest score indicating late-stage OA on day 180 and without indicating apparent variation between subjects. In particular, the lateral femoral condyles were also degenerated, possibly due to localization of weight-bearing from both menisci to the lateral meniscus. Therefore, TMM in minipigs is suitable for reproducible induction of degenerative changes in the femorotibial joints that closely resemble late-stage OA, and is suitable for use in further research.

• Biomolecules & Therapeutics
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• 제27권3호
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• pp.302-310
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• 2019

Melanoma cells have been shown to respond to BRAF inhibitors; however, intrinsic and acquired resistance limits their clinical application. In this study, we performed RNA-Seq analysis with BRAF inhibitor-sensitive (A375P) and -resistant (A375P/Mdr with acquired resistance and SK-MEL-2 with intrinsic resistance) melanoma cell lines, to reveal the genes and pathways potentially involved in intrinsic and acquired resistance to BRAF inhibitors. A total of 546 differentially expressed genes (DEGs), including 239 up-regulated and 307 down-regulated genes, were identified in both intrinsic and acquired resistant cells. Gene ontology (GO) analysis revealed that the top 10 biological processes associated with these genes included angiogenesis, immune response, cell adhesion, antigen processing and presentation, extracellular matrix organization, osteoblast differentiation, collagen catabolic process, viral entry into host cell, cell migration, and positive regulation of protein kinase B signaling. In addition, using the PAN-THER GO classification system, we showed that the highest enriched GOs targeted by the 546 DEGs were responses to cellular processes (ontology: biological process), binding (ontology: molecular function), and cell subcellular localization (ontology: cellular component). Ingenuity pathway analysis (IPA) network analysis showed a network that was common to two BRAF inhibitorresistant cells. Taken together, the present study may provide a useful platform to further reveal biological processes associated with BRAF inhibitor resistance, and present areas for therapeutic tool development to overcome BRAF inhibitor resistance.

• 한국수정란이식학회지
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• 제33권4호
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• pp.305-311
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• 2018

Gonadotropin releasing hormone (GnRH) centrally plays a role in control of the hypothalamic-pituitary-gonadal axis-related hormone secretions in the reproductive neuroendocrine system. In addition, hormone receptors like luteinizing hormone receptor (LHR) are important element for hormones to take effect in target organ. However, ageing-dependent changes in terms of the distribution of GnRH neurons in the brain and LHR expression in the acyclic ovary have not been fully understood yet. Therefore, we comparatively investigated those ageing-dependent changes using young (1-5 months), middle (11-14 months) and old (21-27 months) aged female mice. Whereas a number of GnRH positive fibers and neurons with monopolar or bipolar morphology were abundantly observed in the brain of the young and middle aged mice, a few GnRH positive neurons with multiple dendrites were observed in the old aged mice. In addition, acyclic ovary without repeated development and degeneration of the follicles was shown in the old aged mice than others. LHR expression was localized in theca cells, granulosa cell, corpora lutea and atretic follicle in the ovaries from young and middle aged mice, in contrast, old aged mice had few positive LHR expression on the follicles due to acyclic ovary. However, the whole protein level of LHR was higher in the ovary of old aged mice than others. These results are expected to be used as an important basis on the relationship between GnRH and LHR in old aged animals as well as in further research for reproduction failure.

• 융합신호처리학회논문지
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• 제22권1호
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• pp.20-29
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• 2021

본 연구는 해양레저에서 필요로 하는 정보를 제공해 주는 디바이스의 필요성에 의해서 스마트워치와 풍향풍속계를 개발하였다. 멀티센서를 부착한 해양레저용 스마트워치, 자이로 박스와 풍향풍속계를 기반으로 하여 GPS, 모션, 습도, 온도, 기압, 그리고 심박수 등의 외부 데이터를 수집할 수 있도록 하였다. 그리고 수집된 외부 환경 데이터는 안드로이드 기반의 휴대기기인 스마트폰에서 애플리케이션을 통해 관리할 수 있도록 하였다. 개발된 스마트워치 및 풍향풍속계는 해양 레저 산업의 접근성 증대와 활성화에 기여할 것으로 판단되며 안전과 교육적인 측면에서 해양 정보를 제공해주는 디바이스의 필요성이 크기 때문에 고부가 가치 시장 진출 가능성을 높이고 제품 국산화율을 향상시킬 것으로 예상된다.

• The Plant Pathology Journal
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• 제37권2호
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• pp.182-193
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• 2021

The transcription factor SHE1 was identified as an interacting partner with the cucumber mosaic virus (CMV) 1a protein in the yeast two-hybrid system, by a pull-down assay, and via bimolecular fluorescent complementation. Using fluorescent-tagged proteins and confocal microscopy, the CMV 1a protein itself was found distributed predominantly between the nucleus and the tonoplast membrane, although it was also found in speckles in the cytoplasm. The SHE1 protein was localized in the nucleus, but in the presence of the CMV 1a protein was partitioned between the nucleus and the tonoplast membrane. SHE1 expression was induced by infection of tobacco with four tested viruses: CMV, tobacco mosaic virus, potato virus X and potato virus Y. Transgenic tobacco expressing the CMV 1a protein showed constitutive expression of SHE1, indicating that the CMV 1a protein may be responsible for its induction. However, previously, such plants also were shown to have less resistance to local and systemic movement of tobacco mosaic virus (TMV) expressing the green fluorescent protein, suggesting that the CMV 1a protein may act to prevent the function of the SHE1 protein. SHE1 is a member of the AP2/ERF class of transcription factors and is conserved in sequence in several Nicotiana species, although two clades of SHE1 could be discerned, including both different Nicotiana species and cultivars of tobacco, varying by the presence of particular insertions or deletions.