• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.483-488
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• 2014

In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the $V_{max}$ and specific activity of XynB reached $2,547.7{\mu}mol/mg$ and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at $80^{\circ}C$. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and eventually increasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.

• Korea Journal of Hospital Management
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• v.26 no.3
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• pp.28-42
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• 2021

Purposes: The objective of this study is to analyze service design process of dental clinic preventive service by using 'Double Diamond Design Process' which is user-centered for improving dental clinic management efficiency. Methodology: We examine case study that dental clinic preventive service of local dental clinic. Following the double diamond design four steps(①discover, ②define, ③develop, ④deliver), we use suitable analytic tools in each steps. In discover step, we perform observation and desk research. Next, in define step, affinity diagram, user persona, and user journey map are performed. We then make service blueprint in develop step. Finally, in deliver step, virtual scenario and confirm some outputs which are based on results of former steps are suggested. Findings: As a result of this study, waiting and consulting phases turn out that these phases are in the most trouble than others. Users(patients) have lots of complain and dissatisfaction in these phases. These touch-points will have to be modified Practical Implication: After checking this study result, it could suggest improvement schemes. To apply these, we try to make some scenarios, and finally realize some outputs. Service design has arisen to strengthen the competitiveness by reflecting user's experience and needs. Especially, service design is not typical, it is so flexible that can apply to various situation and objects. We hope that result of this study is made good use for dental clinics in need.

• Journal of Digital Convergence
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• v.16 no.12
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• pp.593-602
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• 2018

The purpose of this study was to investigate the coloring properties of non-mordant dyes by examining local literature on silk fabric dyeing using red-colored natural dyes. Natural dyes can be prepared from the following 8 materials: purple-fleshed sweet potato, Impatiens balsamina, mulberry, fruits of Ligustrum japonicum Thunb, guava leaves, dansam, hibiscus flowers, and pruned branches of Prunus persica. To examine the surface color calculated $L^*$, $a^*$, $b^*$, ${\Delta}E^*$, and K/S value and H V/C. The variables considered in the dyeing experiment were dye concentration, dyeing temperature, dyeing time, and number of dyeing iterations, which were varied to evaluate the dyeing properties and color characteristics. As a result, the abovementioned variables, dye affinity, and red color expression were directly proportional to one another. In this study, it was found that red coloring can be obtained with natural dyes; moreover, excellent dyeing was achieved without the need for repeated dyeing or mordanting processes, which cause environmental pollution.

• Korean Journal of Community Nutrition
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• v.24 no.4
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• pp.290-299
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• 2019

Objectives: This study examined the nutrition teachers' and parents' recognition of environmentally-friendly agricultural products (EAPs) used in school foodservice. Methods: A questionnaire survey was given to 128 school foodservice nutrition teachers in Seongnam and 189 parents from Oct. 16 to Oct. 31, 2018 at Seongnam in Gyeonggi province. The survey included information on the recognition, satisfaction, and improvement of EAPs, and the results of the two groups were compared. Results: A comparison of the recognition of EAPs showed that nutrition teachers knew more about the EAPs and local government support in school foodservice than the parents. On the other hand, the parents were more aware than the nutrition teachers in that children have a higher affinity for EAPs than for general agricultural products in the school foodservice. A comparison of the level of satisfaction with the EAPs by nutrition teachers and parents revealed the nutrition teachers to be significantly more satisfied than parents in terms of the color, taste and nutrition of EAPs. Among the items that should be provided with EAPs, more than 50% of each group of nutrition teachers and parents answered that vegetables must be provided first. Some 70.9% of nutrition teachers and 84.5% of parents were aware of the certification standards of EAPs. The nutrition teachers had showed a slightly higher score than the parents in the certification system (3.51 vs. 3.25). In terms of improving the EAPs, 36.2% of nutrition teachers answered a reasonable price preferentially, whereas 56.4% of parents answered maintaining quality. In the expected effects of using EAPs, 57.9% of nutrition teachers answered an improvement of parents' satisfaction on the school foodservice. On the other hand, 38.0% of parents answered an improvement of children' satisfaction on school foodservice. Conclusions: Nutrition teachers and parents need to be educated on the certification systems that would enhance the trust in EAPs.

• Journal of Species Research
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• v.8 no.1
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• pp.136-159
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• 2019

This study was carried out to investigate the flora of six islands belonging to the Gogunsan Archipelago (i.e., Sinsi-do, Seonyu-do, Munyeo-do, Yami-do, Bian-do, and Duri-do) in the Korean Peninsula. As results of five field surveys from March to October of 2016, we have identified 575 total taxa, representing 527 species, five subspecies, 42 varieties, and one hybrid, placed in 358 genera and 118 families. Of these 575 taxa, four are endemic to Korea, six taxa are listed on the Korean Red List of threatened species, 67 are floristic regional indicator plants, and 74 are invasive alien species. In this study, we compared species richness among the islands, and find that the larger the islands, the higher the species richness. In the case of habitat affinity types, forest species were most common, followed by farmland, seacoast, bare ground and wetland species. From similarity analyses based on the composition of vascular plants, each island did not exhibit either local specificity or unique diversity. On the contrary, the proportion of invasive alien and ruderal species may increase by human activities. Investigations and analyses of island flora such as this are important to assess the current status of the flora, predict future vegetation patterns and the spread of the alien species, and establish managment plans of plant diversity.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.389-391
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• 2022

Efforts by the Ministry of Environment and local governments in Korea are continuing to manage the aging of water supply and sewage buried underground. With the support of the Korea Institute of Environmental Industry and Technology's water and sewage innovation technology development project, it is conducting a project to predict and exchange accidents due to aging, and to apply smart functions to new buried pipes. As one of these studies, this paper proposes the design of a dashboard concept model for digital twin-based smart pipe health monitoring, one of the key features of the entire study. Since remote control and monitoring are one of the main functions, distributed transmission and reception agents are deployed to visualize monitoring situations in real time and to increase user affinity by deploying intuitive UI. To validate the design of this proposed special digital twin based smart pipe state monitoring, we construct the conceptual model level and measure the agent effectiveness to validate its excellence.

• The KIPS Transactions:PartD
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• v.10D no.7
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• pp.1067-1076
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• 2003

Database sharing system (DSS) refers to a system for high performance transaction processing. In DSS, the processing nodes are locally coupled via a high speed network and share a common database at the disk level. Each node has a local memory and a separate copy of operating system. To reduce the number of disk accesses, the node caches database pages in its local memory buffer. In this paper, we propose a dynamic transaction routing algorithm to balance the load of each node in the DSS. The proposed algorithm is novel in the sense that it can support node-specific locality of reference by utilizing the primary copy authority assigned to each node; hence, it can achieve better cache hit ratios and thus fewer disk I/Os. Furthermore, the proposed algorithm avoids a specific node being overloaded by considering the current workload of each node. To evaluate the performance of the proposed algorithm, we develop a simulation model of the DSS, and then analyze the simulation results. The results show that the proposed algorithm outperforms the existing algorithms in the transaction processing rate. Especially the proposed algorithm shows better performance when the number of concurrently executed transactions is high and the data page access patterns of the transactions are not equally distributed.

• Journal of Pharmacopuncture
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• v.18 no.2
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• pp.7-18
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• 2015

Objectives: Lawsone (1,4 naphthoquinone) is a non redox cycling compound that can be catalyzed by DT diaphorase (DTD) into 1,2,4-trihydroxynaphthalene (THN), which can generate reactive oxygen species by auto oxidation. The purpose of this study was to evaluate the toxicity of the phytomarker 1,4 naphthoquinone and its metabolite THN by using the molecular docking program AutoDock 4. Methods: The 3D structure of ligands such as hydrogen peroxide ($H_2O_2$), nitric oxide synthase (NOS), catalase (CAT), glutathione (GSH), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) were drawn using hyperchem drawing tools and minimizing the energy of all pdb files with the help of hyperchem by $MM^+$ followed by a semi-empirical (PM3) method. The docking process was studied with ligand molecules to identify suitable dockings at protein binding sites through annealing and genetic simulation algorithms. The program auto dock tools (ADT) was released as an extension suite to the python molecular viewer used to prepare proteins and ligands. Grids centered on active sites were obtained with spacings of $54{\times}55{\times}56$, and a grid spacing of 0.503 was calculated. Comparisons of Global and Local Search Methods in Drug Docking were adopted to determine parameters; a maximum number of 250,000 energy evaluations, a maximum number of generations of 27,000, and mutation and crossover rates of 0.02 and 0.8 were used. The number of docking runs was set to 10. Results: Lawsone and THN can be considered to efficiently bind with NOS, CAT, GSH, GR, G6PDH and NADPH, which has been confirmed through hydrogen bond affinity with the respective amino acids. Conclusion: Naphthoquinone derivatives of lawsone, which can be metabolized into THN by a catalyst DTD, were examined. Lawsone and THN were found to be identically potent molecules for their affinities for selected proteins.

• Fire Science and Engineering
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• v.32 no.6
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• pp.84-90
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• 2018

Chemical accidents occur in various forms, such as explosions, leaks, spills, and fires. In particular, chemical accidents caused by fires seriously affect the surrounding air environment due to soot, causing anxiety to the residents. Therefore, it is important to identify the causative substances quickly and examine the influence of air quality in the surrounding area. In this paper, proton transfer reaction-time of flight mass spectrometry(PTR-ToFMS) was used to identify the causative material in a fire and monitor the air quality in real time. This analyzer is capable of real-time analysis with a rapid response time without sample collection and pretreatment. In addition, it is suitable for quantitative and qualitative analysis of most volatile organic compounds with high hydrogen affinity, to identify the cause of fire and examine the influence of ambient air. In April 2018, when a local fire occurred, methanol, acetone, and methyl ethyl ketone were the main causative agents in PTR-ToFMS.

• World Technopolis Review
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• v.8 no.1
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• pp.59-70
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• 2019

Where is a better place to live? In the coming era, this should be more than simply a livable place. It should be an adaptable place that has a flexible system adaptable to any new situation in terms of diversity. Customization and real-time operation are needed in order to realize this technologically. We expect a smart city to have a flexible system that applies technologies of self-monitoring and self-response, thereby being a promising city model towards being a better place to live. Energy demand and supply is a crucial issue concerning our expectations for the flexible system of a smart city because it is indispensable to comfortable living, especially city living. Although it may seem that energy diversification, such as the energy mix of a country, is a matter of overriding concern, the central point is the scale of place to build grids for realizing sustainable urban energy systems. A traditional hard energy path supported by huge centralized energy systems based on fossil and nuclear fuels on a national scale has already faced difficult problems, particularly in terms of energy flexibility/resilience. On the other hand, an alternative soft energy path consisting of small diversified energy systems based on renewable energy sources on a local scale has limitations regarding stability, variability, and supply potential despite the relatively light economic/technological burden that must be assumed to realize it. As another alternative, we can adopt a holonic path incorporating an alternative soft energy path with a traditional hard energy path complimentarily based on load management. This has a high affinity with the flexible system of a smart city. At a system level, the purpose of all of the paths mentioned above is not energy itself but the service it provides. If the expected energy service is fixed, the conclusive factor in choosing a more appropriate system is accessibility to the energy service. Accessibility refers to reliability and affordability; the former encompasses the level of energy self-sufficiency, and the latter encompasses the extent of energy saving. From this point of view, it seems that the small diversified energy systems of a soft energy path have a clear advantage over the huge centralized energy systems of a hard energy path. However, some insuperable limitations still remain, so it is reasonable to consider both energy systems continuing to coexist in a multiplexing energy system employing a holonic path to create and maintain reliable and affordable access to energy services that cover households'/enterprises' basic energy needs. If this is embodied in a smart city concept, this is nothing else but smart energy inclusion. In Japan, following the Fukushima nuclear accident in 2011, a trend towards small diversified energy systems of a soft energy path intensified in order to realize a nuclear-free society. As a result, the Government of Japan proclaimed in its Fifth Strategic Energy Plan that renewable energy must be the main source of power in Japan by 2050. Accordingly, Sony vowed that all the energy it uses would come from renewable sources by 2040. In this situation, it is expected that smart energy inclusion will be achieved by the Japanese version of a smart grid based on the concept of a minimum cost scheme and demand response.