• 생명과학회지
• /
• 제14권5호
• /
• pp.770-777
• /
• 2004

a-Tropomyosin (TM)의 아미노(N) 말단 구조의 중요성을 확인하기 위하여 N 말단에 알라닌 아미노산 잔기 하나를 첨가한 재조합 Ala-TM을 제조하였다. Ala-TM을 대장균에서 대량발현 시켜 정제한 후, N 말단이 아세틸화된 근육TM및 N말단에 알라닌-세린 잔기를 첨가한 AS-TM과 N말단이 비아세틸화된 TM등의 재조합 TM과 기능을 비교하였다. Ala-TM은 비아세틸화된 TM보다 액틴친화력이 현저히 증가했으나, 근육 및 AS-TM 보다는 약 3배정도 약하게 액틴에 결합하였다. 근육 TM, AS-TM,그리고 Ala-TM모두가 myosin 51의 농도가 낮을 때 ATPase 활성을 억제하였고 농도가 높을 때 촉진하였으나, 억제와 촉진의 정도는 서로 차이가 있었으며 비아세틸화된 TM은 억제하지 않았다. 이들 결과는 N말단 구조가 TM의 기능을 결정하는 중요한 요소임을 나타내며 TM의 온전한 기능을 위해서는 아세틸화된 N 말단이 필요하다는 것을 의미한다.

• Archives of Pharmacal Research
• /
• 제10권3호
• /
• pp.184-187
• /
• 1987

ln order to develope anti-inflammatory glucocorticoids for local use without systemic side-effects, ester and amide derivatives of 20$\xi$-dihydroprednisolonic acid have been prepared. When binding affinities of these compounds to glucocorticoid receptor of rat liver cytosol were compared, all a-isomer at C-20 showed higher binding affinities than the corres¬ponding $\beta$-isomer. The size of the substituents at C-21 had significant influences on binding affinities, which were related with their lipophilicity.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제2권2호
• /
• pp.93-101
• /
• 2002

We have proposed and fabricated two lateral type field emission diodes, poly-Si emitter by utilizing the local oxidation of silicon (LOCOS) and GaN emitter using metal organic chemical vapor deposition (MOCVD) process. The fabricated poly-Si diode exhibited excellent electrical characteristics such as a very low turn-on voltage of 2 V and a high emission current of $300{\;}\bu\textrm{A}/tip$ at the anode-to-cathode voltage of 25 V. These superior field emission characteristics was speculated as a result of strong surface modification inducing a quasi-negative electron affinity and the increase of emitting sites due to local sharp protrusions by an appropriate activation treatment. In respect, two kinds of procedures were proposed for the fabrication of the lateral type GaN emitter: a selective etching method with electron cyclotron resonance-reactive ion etching (ECR-RIE) or a simple selective growth by utilizing $Si_3N_4$ film as a masking layer. The fabricated device using the ECR-RIE exhibited electrical characteristics such as a turn-on voltage of 35 V for $7\bu\textrm{m}$ gap and an emission current of~580 nA/l0tips at anode-to-cathode voltage of 100 V. These new field emission characteristics of GaN tips are believed to be due to a low electron affinity as well as the shorter inter-electrode distance. Compared to lateral type GaN field emission diode using ECR-RIE, re-grown GaN emitters shows sharper shape tips and shorter inter-electrode distance.

• ETRI Journal
• /
• 제35권2호
• /
• pp.311-320
• /
• 2013

In most spectral clustering approaches, the Gaussian kernel-based similarity measure is used to construct the affinity matrix. However, such a similarity measure does not work well on a dataset with a nonlinear and elongated structure. In this paper, we present a new similarity measure to deal with the nonlinearity issue. The maximum flow between data points is computed as the new similarity, which can satisfy the requirement for similarity in the clustering method. Additionally, the new similarity carries the global and local relations between data. We apply it to spectral clustering and compare the proposed similarity measure with other state-of-the-art methods on both synthetic and real-world data. The experiment results show the superiority of the new similarity: 1) The max-flow-based similarity measure can significantly improve the performance of spectral clustering; 2) It is robust and not sensitive to the parameters.

• Journal of Electrical Engineering and Technology
• /
• 제13권6호
• /
• pp.2511-2520
• /
• 2018

In this study, Multi-Radial Basis Function Support Vector Machine (Multi-RBF SVM) classifier is introduced based on a composite kernel function. In the proposed multi-RBF support vector machine classifier, the input space is divided into several local subsets considered for extremely nonlinear classification tasks. Each local subset is expressed as nonlinear classification subspace and mapped into feature space by using kernel function. The composite kernel function employs the dual RBF structure. By capturing the nonlinear distribution knowledge of local subsets, the training data is mapped into higher feature space, then Multi-SVM classifier is realized by using the composite kernel function through optimization procedure similar to conventional SVM classifier. The original training data set is partitioned by using some unsupervised learning methods such as clustering methods. In this study, three types of clustering method are considered such as Affinity propagation (AP), Hard C-Mean (HCM) and Iterative Self-Organizing Data Analysis Technique Algorithm (ISODATA). Experimental results on benchmark machine learning datasets show that the proposed method improves the classification performance efficiently.

• 문화기술의 융합
• /
• 제10권3호
• /
• pp.497-502
• /
• 2024

지역문화는 지역의 전통과 현대의 변화 속에서 가치를 함축하여 브랜드화에 관점으로 접근하여 시민들의 문화 역량을 높이는 역할을 하고 있다. 따라서 본 연구는 지역문화 특성이 문화 브랜드 자산 구성요인에 영향을 분석하기 위하여 지역문화 특성의 지역문화 고유성, 지역문화 정체성, 지역문화 장소성, 지역문화 연대성을 독립변수로, 문화브랜드 자산 구성요인의 브랜드 충성도, 브랜드 재인, 지각된 품질, 브랜드 이미지를 종속변수로 하여 연구모형을 설정하였다. 연구가설을 검정하여 분석한 결과 지역문화 특성이 문화브랜드 자산 구성요인에 영향을 미치고 있어 지역문화에 대한 인식변화와 전통문화의 홍보가 필요한 것으로 나타났다. 그리고 여주시는 세계문화유산 여주 영릉이 브랜드의 재인과 브랜드 이미지를 위한 콘텐츠의 융합과 문화정책의 전문화를 통하여 전통과 지역문화를 융합한 축제를 계획할 필요가 있으며 시민과 함께하는 방향으로 전환하는 것이 시급하다. 그리고 지역문화 애호도에 따른 문화브랜드 자산의 형성에 관한 연구를 확대하는 것이 바람직한 것으로 나타났다.

• International Journal of Oral Biology
• /
• 제30권1호
• /
• pp.31-37
• /
• 2005

Treponema maltophilum, a Group IV oral spirochete, is associated with periodontitis and endodontic infections. In this study we analyzed the functional role of the major surface protein of this organism (MspA) in human gingival fibroblasts (HGFs). The full-length gene encoding MspA was cloned and expressed in Escherichia coli by using the expression vector pQE-30. The recombinant protein (rMspA) was purified by affinity chromatography with nickel-nitrilotriacetic acid agarose and possible contamination of E. coli endotoxin in rMspA was removed by using polymyxin B-agarose. rMspA significantly induced the expression of pro inflammatory cytokines like IL-6 and IL-8 and intercellular adhesion molecule (ICAM)-1 in HGFs, when analyzed by reverse transcription-PCR, flow cytometry, and enzyme-linked immunosorbent assay. Our results indicate that MspA of T. maltophilum may play an important role in amplifying the local immune response by upregulating the expression of proinflammatory cytokines and ICAM-1.

• International Journal of Computer Science & Network Security
• /
• 제23권2호
• /
• pp.31-38
• /
• 2023

In the current Internet system, there are many problems using anonymity of the network communication such as personal information leaks and crimes using the Internet system. This is why TCP/IP protocol used in Internet system does not have the user identification information on the communication data, and it is difficult to supervise the user performing the above acts immediately. As a study for solving the above problem, there is the study of Policy Based Network Management (PBNM). This is the scheme for managing a whole Local Area Network (LAN) through communication control for every user. In this PBNM, two types of schemes exist. As one scheme, we have studied theoretically about the Destination Addressing Control System (DACS) Scheme with affinity with existing internet. By applying this DACS Scheme to Internet system management, we will realize the policy-based Internet system management. In this paper, required functions in the PBNM Scheme for multiple domains as cyber physical system that utilizes data science and AI is examined.

• International Journal of Computer Science & Network Security
• /
• 제23권1호
• /
• pp.71-77
• /
• 2023

In the current Internet system, there are many problems using anonymity of the network communication such as personal information leaks and crimes using the Internet system. This is why TCP/IP protocol used in Internet system does not have the user identification information on the communication data, and it is difficult to supervise the user performing the above acts immediately. As a study for solving the above problem, there is the study of Policy Based Network Management (PBNM). This is the scheme for managing a whole Local Area Network (LAN) through communication control for every user. In this PBNM, two types of schemes exist. As one scheme, we have studied theoretically about the Destination Addressing Control System (DACS) Scheme with affinity with existing internet. By applying this DACS Scheme to Internet system management, we will realize the policy-based Internet system management. In this paper, to realize it, concept of the Internet PBNM Scheme is proposed as the final step.

• International Journal of Computer Science & Network Security
• /
• 제23권11호
• /
• pp.1-7
• /
• 2023

In the current Internet system, there are many problems using anonymity of the network communication such as personal information leaks and crimes using the Internet system. This is why TCP/IP protocol used in Internet system does not have the user identification information on the communication data, and it is difficult to supervise the user performing the above acts immediately. As a study for solving the above problem, there is the study of Policy Based Network Management (PBNM). This is the scheme for managing a whole Local Area Network (LAN) through communication control for every user. In this PBNM, two types of schemes exist. As one scheme, we have studied theoretically about the Destination Addressing Control System (DACS) Scheme with affinity with existing internet. By applying this DACS Scheme to Internet system management, we will realize the policy-based Internet system management. In this paper, basic system design for PBNM scheme for multi-domain management utilizing data science and AI is proposed.