• Title/Summary/Keyword: Lobelia chinensis

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Micropropagation of Lobelia chinensis Lour.: Influence of Medium Parameters on Plant Regeneration, Antioxidant Activity, and Secondary Metabolite Accumulation

  • Xinlei Bai;Han-Sol Lee;Hosakatte Niranjana Murthy;Hyuk-Joon Kwon;Soo-Ho Yeon;Jae-Yeong Ju;So-Young Park
    • Korean Journal of Plant Resources
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    • v.37 no.3
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    • pp.225-234
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    • 2024
  • Chinese lobelia (Lobelia chinensis Lour.) is an important medicinal plant that is used in traditional Chinese, Korean, and Japanese medicine. The goal of the current study was to develop an in vitro propagation technique for Lobelia chinensis. We have examined the effects of different media compositions on the regeneration of shoots from nodal cultures of Lobelia chinensis, including Murashige and Skoog (MS), Gamborg (B5), Schenk and Hildebrandt (SH), Woody plant (WPM), Chu (N6), and Nitsch and Nitsch (NLN) media. Similar to this, shoot regeneration was examined using MS medium of double (2.0), full (1.0), half (0.5), and quarter (0.25) strengths. The regeneration of shoots was also examined with additions of 0, 1, 3, 5, and 7% (w/v) sucrose to MS media. For axillary shoot regeneration, full-strength MS medium supplemented with 3% (w/v) sucrose was shown to be the most effective of all the evaluated factors. On this medium, nodal explants optimally regenerated 4.5 shoots per explant and subsequently shoots involved in rooting on the same medium. The regenerated plants possessed abundant phenolics, flavonoids, and DPPH, ABTS, and FRAP antioxidant activities. High performance liquid chromatographic examination (HPLC) of the regenerated plants revealed an accumulation of myricetin and catechin in higher amounts.

Inhibition of Interleukin-4 and β-Hexosaminidase Release in RBL-2H3 Cells by Compounds Isolated from Lobelia chinensis

  • Kim, Tae Young;Jo, Beom-Geun;Park, No-Jun;Park, Young-Hun;Kim, Su-Nam;Yang, Min Hye
    • Natural Product Sciences
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    • v.27 no.4
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    • pp.251-256
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    • 2021
  • Lobelia chinensis Lour. has commonly been used in traditional Chinese medicine for the treatment of antidote, diuretic, diarrhea, and inflammation. This study aimed to identify the active compounds in an aqueous extract of L. chinensis responsible for its anti-atopic effect in vitro using RBL-2H3 cells. A chemical investigation of secondary metabolites in an aqueous extract of L. chinensis led to the isolation of nine chemical constituents, which included the four marker compounds, and these were evaluated for their inhibitory effects on IL-4 mRNA expression and the release of β-hexosaminidase in propidium iodide-induced RBL-2H3 cells. We found diosmetin and fraxidin inhibited cellular IL-4 mRNA expression, and that diosmetin and 6,8-dimethoxycoumarin inhibited DNP-specific IgE-induced degranulation in these cells. Our study suggests that diosmetin, fraxidin, and 6,8-dimethoxycoumarin are potential candidates for the treatment of atopic diseases.

Chemical Control of Weed for Rapes ( Brassica napus L. ) (제초제에 의한 유채밭 잡초방제)

  • 안계수;권병선;김상곤;정동희
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.3
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    • pp.186-191
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    • 1995
  • This study was conducted to evaluate the effect of herbicides on weed control, growth characteristics and yield in rapes, after direct seeding it to the field. All herbicides treated had no effect on the emergence period, bolting rate of rapes. The major weeds were Cerastium holosteoides var. hallaisanense, Stellaria media Villars, Larnium ampleicaule L., Lobelia chinensis Lour., Geranium wilfordii Maxim. and Capsellu bursa-pastoris (L.) Medicus. Rapes yield were increased somewhat more with alachlor-G, herbicide than the other hehicides and by hand weeding. Alachlor-G and alachlor-Ec were had no i j u r y but butachlor- G and simajin-Wp were slightly harmful for the rapes with recommended concentration. On the other hand all hehicides were harmful in the double dosage level.

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Studios on the Host Range of Cucumber Mosaic Virus in Korea (한국에서의 오이모자익 바이러스의 기주범위에 관한 연구)

  • Chung B. J.;Park H. C.;Lee S. H.
    • Korean journal of applied entomology
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    • v.14 no.4 s.25
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    • pp.185-192
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    • 1975
  • Various plant species, as many as 145 species in 43 families, were tested for susceptibility to the ordinary strain of the cucumber nosaic virus for two years from 1973 to 1974. Inoculations were made by mechanical method using carborundum. Plants in 71 species belonging to 27 families were infected. Of these species, systemic mosaic developed on the new leaves of plants in 57 species belonging to 24 families. Twenty-four species of plants, previously not reported as hosts of the CMV, were found to be infected in this experiment. These are Stellaria aquatica, Achyrauthes japonica, Agerratum houstonianum, Centipeda minima, Gillardia pulchella, Henisteptalyrate, Ixeris dentata, Saussurea uchiyamana, Brassica campestris, Lepidiumapetalum, Lobelia chinensis, Chenopodium bryoniaefolium, Carex neofilipes, Acalypha austalis, Amphicarpaea edgeworthii, Lotus corniculatus var japonicus, Phaseolus angularis, Sedum aizoom var heterodontum, Mosla punctulata, Perilla frutescens var japonica, Teucrium japonicum,. Linum usitatissimum, Mazus japonicus, Verbena hybrida. Twenty-three species reported to be susceptible by previous workers, but negative results were obtained in our experiment with Allium cepa, Celosia cristat, Daucus carota var. sativa, Artemisia asiatica, Callistenphus chinensis, Erigeron canadensis, Helianthusannuus, Tagetes eracta, Impatiens balsamina, Raphanus sativus, Ipomea batatas, Glycine max, Phaseolus vulgaris, Lilium longifolium, Papaver gomniferum, Sorghum vulgare, Triticum aestivum, Zea mays, Rumex coreanus, Potulaca grandiflora.

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The Study on Flora and Distribution Characteristics of Orchidaceae on Moor

  • Rho, Jae-Hyun
    • Journal of Environmental Science International
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    • v.12 no.2
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    • pp.179-184
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    • 2003
  • Total 7 moors, Yong-neup on Mt. Daeam, Chilbo Mt. moor, Dogapsa-neup on Mt. Wolchul, Wangdeungiae-neup on Mt. Jiri, Mujechi-neup on Mt. Jungjok, Buljae-neup on Mt. Kyungak and Geoinri-neup on Dongsang, were investigated by literatures and survey. Distribution, conditions, and similarity of dominant species and Orchidaceae on moor were analyzed. The results are follows; There are total 46 wetland plant species observed more than twice from 7 moors. The plants classified as constancy class V, which are appeared more than 80%, are Iris ensata var. spontane, Juncus effusus var. decipiens, Sanguisorba tenuifolia var. alba, Habenaria linearifolia, Sium ninsi, Lobelia sessilifolia, Lastrea theiyptoris, and Eupatorium chinensis var. simplicifolium. Highly appearing Orchidaceae, which was classified to constancy class IV plant, are Habenaria linearifolia, Pogonia japonica, Eipactis thunbergii, and Platanthera mandarinorum. They are the typical index plants of the moors. Habitual environment of Habenaria radiate and Platanthera hologlottis can be affected by environmental aspects such as latitude, altitude and the water quality. However those of Habenaria linearifolia, Epipactis thunbergii, and Pogonia japonica are not affected by latitude and altitude, but may be determined by water condition of wetland. As the result of similarity index analysis for appearance of Orchidaceae identifying each moors in the view of species composition, swamps at Dogapsa-neup and Buljae-neup are very similar. However Yong-neup is totally different from those of Dogapsa-neup and Buljae-neup. Yong-neup had shown the high similarity indices of 0.85 and 0.75 with Wangdeungiae-neup and Mujechi-neup, respectively. Growth properties of wild Orchidaceae at wetland were almost similar. Platanthera mandarinorum and Liparis kumokiri had characteristics of good adaptability. Most of them need light. Pogonia was the only long-day plant. The flowering time of these Orchidaceae was from May to September.

Conservation Management Methods and Classification Type of Flora in the Bongamsa(Temple) Area, Mungyeong (문경 봉암사 일대의 식물상 유형별 분류 및 보전관리방안)

  • Oh, Hyun-Kyung;Han, Yun-Hee;Choi, Song-Hyun
    • Korean Journal of Environment and Ecology
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    • v.25 no.4
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    • pp.447-469
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    • 2011
  • The flora of Bongamsa (Temple) area, Mungyeong were listed 552 taxa; 99 families, 303 genera, 468 species, 1 subspecies, 70 varieties and 13 forms. Based on the rare plants (IUCN), total 12 taxa; Crypsinus hastatus (Polypodiaceae), Eranthis stellata (Ranunculaceae), Cnidium tachiroei (Umbelliferae), Monotropa hypopithys (Pyrolaceae), Rhododendron micranthum (Ericaceae), Salvia chanroenica (Labiatae), Gastrodia elata (Orchidaceae), etc. Based on the endemic plants, total 11 taxa; Asarum chungbuensis, Aconitum pseudolaeve, Corydalis maculata, Lonicera subsessilis, Aster koraiensis, Saussurea seoulensis, etc. Based on the specific plants by floral region were total 48 taxa (8.7% of all 552 taxa of vascular plants); 2 taxa in class V, 5 taxa (Cimicifuga heracleifolia, Wistaria floribunda, Elsholtzia angustifolia, etc.) in class IV, 10 taxa (Betula davurica, Spiraea chinensis, Acer barbinerve, Vaccinium koreanum, Syringa reticulata var. mandshurica, etc.) in class III, 7 taxa (Potentilla dickinsii, Viola orientalis, Ostericum melanotilingia, Melica nutans, Veratrum parulum, etc.) in class II, 24 taxa (Camptosorus sibiricus, Cephalotaxus koreana, Betula schmidtii, Ilex macropoda, Tilia amurensis, Triadenum japonica, Lobelia sessilifolia, etc.) in class I. Based on the naturalized plants, total 21 taxa (Silene armeria, Oenothera lamarckiana, Symphytum officinale, Erechtites hieracifolia, etc.) and naturalization rate was 3.8% of all 552 taxa of vascular plants. The flora of importance in the Bongamsa, rare plants were Crypsinus hastatus, Eranthis stellata, Gastrodia elata, Monotropa uniflora, Monotropa hypopithys, Rhododendron micranthum and Cnidium tachiroei, Iris ensata var. spontanea in intermediate marsh and alien species classify into planted species of Bongamsa, area for investigation of afforestation and artificial forests, introduced of naturalized plants.