• Biomolecules & Therapeutics
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• v.11 no.4
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• pp.224-231
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• 2003

The aim of this study was to investigate the effect of resveratrol on the oxidative stress-induced inhibition of gap junctional intercellular communication in HaCaT keratinocytes. Anti-oxidative activity of resveratrol was measured by $\alpha,\alpha$-diphenyl-$\beta$-picrylhydrazyl assay and dichlorodihydrofluorescein diacetate oxidation assay. Gap junctional intercellular communication in HaCaT keratinocytes was assessed using the scrape loading/dye transfer technique. Western blots and reverse transcription-polymerase chain reaction were also analyzed for connexin 43 protein and mRNA expression, respectively. Resveratrol scavenged directly the stable $\alpha,\alpha$-diphenyl-$\beta$-picrylhydrazyl radical over a concentration range of 4 mg/ml ($78.2{\pm}2.7$% of control) to 500 mg/ml ($29.9{\pm}4.2$% of control) and decreased the intracellular reactive oxygen species induced by ultraviolet A (UVA) irradiation ($89.3{\pm}1.1$% of UVA group), ultraviolet B (UVB) irradiation ($70.9{\pm}1.7$% of UVB group) and 12-0-tet-radecanoylphorbol-13-acetate (TPA, $48.3{\pm}1.1$% of TPA group), respectively. UVA irradiation and TPA markedly reduced gap junctional intercellular communication, which was restored by resveratrol. There were no significant differences in the level of connexin 43 protein and mRNA expression among any of the experimental groups. Our data suggests that resveratrol has the protective effect on the oxidative stress-induced inhibition of gap junctional intercellular communication in HaCaT keratinocytes, and this protection is likely due to the scavenging of reactive oxygen species.

• The Korean Journal of Nuclear Medicine
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• v.37 no.1
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• pp.43-52
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• 2003

Microarray technology allows the simultaneous analysis of gene expression patterns of thousands of genes, in a systematic fashion, under a similar set of experimental conditions, thus making the data highly comparable. In some cases arrays are used simply as a primary screen loading to downstream molecular characterization of individual gene candidates. In other cases, the goal of expression profiling is to begin to identify complex regulatory networks underlying developmental processes and disease states. Microarrays were originally used with ceil lines or other simple model systems. More recently, microarrays have been used in the analysis of more complex biological tissues including neural systems and the brain. The application of cDNA arrays in neuropsychiatry has lagged behind other fields for a number of reasons. These include a requirement for a large amount of input probe RNA In fluorescent-glass based array systems and the cellular complexity introduced by multicellular brain and neural tissues. An additional factor that impacts the general use of microarrays in neuropsychiatry is the lack of availability of sequenced clone sets from model systems. While human cDNA clones have been widely available, high qualify rat, mouse, and drosophilae, among others are just becoming widely available. A final factor in the application of cDNA microarrays in neuropsychiatry is cost of commercial arrays. As academic microarray facilitates become more commonplace custom made arrays will become more widely available at a lower cost allowing more widespread applications. in summary, microarray technology is rapidly having an impact on many areas of biomedical research. Radioisotope-nylon based microarrays offer alternatives that may in some cases be more sensitive, flexible, inexpensive, and universal as compared to other array formats, such as fluorescent-glass arrays. In some situations of limited RNA or exotic species, radioactive membrane microarrays may be the most practical experimental approach in studying psychiatric and neurodegenerative disorders, and other complex questions in the brain.

• Applied Chemistry for Engineering
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• v.27 no.2
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• pp.199-204
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• 2016

The adsorption of Rhodamine-B dye using granular activated carbon from aqueous solution was investigated. Adsorption experiments were carried out as a function of the adsorbent dose, pH initial concentration, contact time and temperature. The equilibrium adsorption data showed a good fit to Langmuir isotherm model. Based on the estimated Langmuir separation factor ($R_L$ = 0.0164~0.0314), our adsorption process could be employed as an effective treatment method. The kinetics of adsorption followed the pseudo first order model. Also, the negative values of Gibbs free energy (-4.51~-13.44 kJ/mol) and positive enthalpy (128.97 kJ/mol) indicated that the adsorption was spontaneous and endothermic process. The isosteric heat of adsorption increased with increase in the surface loading indicating lateral interactions between the adsorbed dye molecules.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.1
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• pp.125-129
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• 2010

Stateless computing supports a mobility of computing environment easily. It is becoming a major technology for securing personal user's information on shared computing environment. With the advance of virtualization technology and cloud computing, stateless computing is an essential part of personal computing environment connectivity (user's setting and data is stored in remote server or some storage, and it can be restored at any computing environment) In this paper, we propose uPC player that supports stateless computing execution environment on Windows. uPC player provides Windows operating system to user by using an uPC OS virtualization module. In this paper, we leverage how uPC player is designed and implemented for supporting a stateless computing execution environment. uPC player provides a desktop switch between host-system execution environment and uPC virtual execution environment. And it needs just one second for loading uPC virtual execution environment by using OS virtualization-based technique.

• Journal of the Korea institute for structural maintenance and inspection
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• v.7 no.4
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• pp.191-199
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• 2003

The inelastic response of many structural steel and reinforced concrete structures subject to extreme loadings can be characterized by elastoplastic behaviors. Although excursion beyond the elastic range is usually not permitted under normal conditions of service, the extent of permanent damage a structure may sustain when subjected to extreme conditions, such as severe blast or earthquake loading, is frequently of interest to the engineer. A blast is usually the result of an explosion defined as a "sudden expansion". This paper discusses the basic concept that defines blast loadings on structures and corresponding elastoplastic structural response (displacement, velocity, and acceleration) and try to explain a crack propagation of concrete in sudden expansion. According to nonlinear finite element analysis, the crack forms of static and dynamic states displayed different in RC structural members. This paper also provides useful data for the dynamic fracture analysis of RC frame structures.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.2
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• pp.31-39
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• 2012

Recently new construction and reconstruction of the bridge have been required to minimize traffic congestion, environmental disadvantage, to reduce the period of construction, and to improve the quality and workability during the construction. For this reason, the application of modular bridge system, which is assembly of the structural members, is necessary to prepare for near future. Fall of girders can occur at the moment to connect between precast girders during the construction, so appropriate cross beams should be installed to solve the mentioned problem. In this study, understanding the structural characteristics and domestic and international case of cross beam, alternative cross beam system for modular bridge was developed. To inspect the structural characteristics of the alternative system, specimens were built and static loading test was performed. Afterward, the behavior of cross beam interms of joints and load distribution was observed. Experimental results were analyzed and compared with each data. Therefore, the appropriate cross beam system for modular bridge will be chosen and proposed in this paper.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.4
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• pp.51-61
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• 2003

This research is a park of a research program to develope a new design method for reinforced concrete bridge columns under axial load and cyclic lateral load. The objectives of this paper are to investigate the relationship between curvature ductility and displacement ductility and to propose a correlation equation for designing of reinforced concrete bridge columns under axial load and cyclic lateral load. Computer program NARCC was used for parametric study, which was proved to provide good and conservative analytical result especially for deformation capacity and ductility factor compared with test result. A total of 7,200 spirally reinforced concrete columns were selected considering the main variables such as section diameter, aspect ratio, concrete strength, yielding strength of longitudinal and confinement steel, longitudinal steel ratio, axial load ratio, and confinement steel ratio. A new equation between curvature ductility factor displacement ductility factor with the aspect ratio was proposed by investigation of 21,600 data produced from the selected column models by applying 3 different definitions of yield displacement.

• Advances in nano research
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• v.8 no.1
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• pp.83-94
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• 2020

An analytical formulation and solution process for the buckling analysis of porous magneto-electro-elastic functionally graded (MEE-FG) beam via different thermal loadings and various boundary conditions is suggested in this paper. Magneto electro mechanical coupling properties of FGM beam are taken to vary via the thickness direction of beam. The rule of power-law is changed to consider inclusion of porosity according to even and uneven distribution. Pores possibly occur inside FGMs due the result of technical problems that lead to creation of micro-voids in these materials. Change in pores along the thickness direction stimulates the mechanical and physical properties. Four-variable tangential-exponential refined theory is employed to derive the governing equations and boundary conditions of porous FGM beam under magneto-electrical field via Hamilton's principle. An analytical model procedure is adopted to achieve the non-dimensional buckling load of porous FG beam exposed to magneto-electrical field with various boundary conditions. In order to evaluate the influence of thermal loadings, material graduation exponent, coefficient of porosity, porosity distribution, magnetic potential, electric voltage and boundary conditions on the critical buckling temperature of the beam made of magneto electro elastic FG materials with porosities a parametric study is presented. It is concluded that these parameters play remarkable roles on the buckling behavior of porous MEE-FG beam. The results for simpler states are proved for exactness with known data in the literature. The proposed numerical results can serve as benchmarks for future analyses of MEE-FG beam with porosity phases.

• Structural Engineering and Mechanics
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• v.73 no.5
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• pp.529-542
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• 2020

As limited well-documented experimental data are available for assessing the attributes of different deformation components of flanged walls, few appropriate models have been established for predicting the inelastic responses of flanged walls, especially those of asymmetrical flanged walls. This study presents the experimental results for three large-scale T-shaped reinforced concrete walls and examines the variations in the flexural, shear, and sliding components of deformation with the total deformation over the entire loading process. Based on the observed deformation behavior, a simple model based on moment-curvature analysis is established to estimate flexural deformations, in which the changes in plastic hinge length are considered and the deformations due to strain penetration are modeled individually. Based on the similar gross shapes of the curvature and shear strain distributions over the wall height, a proportional relationship is established between shear displacement and flexural rotation. By integrating the deformations due to flexure, shear, and strain penetration, a new load-deformation analytical model is proposed for flexure-dominant flanged walls. The proposed model provides engineers with a simple, accurate modeling tool appropriate for routine design work that can be applied to flexural walls with arbitrary sections and is capable of determining displacements at any position over the wall height. By further simplifying the analytical model, a simple procedure for estimating the ultimate displacement capacity of flanged walls is proposed, which will be valuable for performance-based seismic designs and seismic capacity evaluations.

• Geomechanics and Engineering
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• v.19 no.2
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• pp.115-126
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• 2019

The long-term settlement characteristics for the cast-in-place bored pile in the deep-thick soft soil are investigated by post-grouting field tests. Six cast-in-place bored engineering piles and three cast-in-place bored test piles are installed to study the long-term settlement characteristics. Three post-grouting methods (i.e., post-tip-grouting, post-side-grouting, and tip and side post-grouting) are designed and carried out by field tests. Results of the local test show that decreased settlements for the post-side-grouted pile, the post-tip-grouted pile and the tip and side post-grouted pile are 22.2%~25.8%, 30.10%~35.98% and 32.40%~35.50%, respectively, compared with non-grouted piles. The side friction resistance for non-grouted piles, post-side-grouted pile, post-tip-grouted pile and the tip and side post-grouted pile undertakes 89.6~91.3%, 94.6%, 92.4%~93.0%, 95.7% of the total loading, respectively. At last, the parameters back analysis method and numerical calculation are adopted to predict the long-term settlement characteristics of the cast-in-place bored pile in the deep-thick soft soil. Determined Bulk modulus (K) and a creep parameter (Ks) are used for the back analysis of the long-term settlement of the post-grouted pile. The settlement difference between the back analysis and the measurement data is about 1.11%-7.41%. Long-term settlement of the post-grouted piles are predicted by the back analysis method, and the predicted results show that the settlement of the post-grouted pile are less than 6 mm and will be stable in 30 days.