• Title/Summary/Keyword: Liver and Kidney

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Effects of Dietary Folate Intake on Plasma and Tissue Folate Concentrations in Rats (식이 엽산수준이 흰쥐의 혈장과 조직의 엽산함량에 미치는 영향)

  • 장남수
    • Journal of Nutrition and Health
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    • v.31 no.3
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    • pp.271-278
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    • 1998
  • Folate coenzymes are involved in one-carbon transfer reactions needed for the synthesis of nucleic acids, amino acids, and proteins which are very important for cell proliferation and differentiation. To investigate the effects of dietary folate content on plasma and tissue folate concentrations and on folate excretions in urine and feces, male Sprague-Dawley rats were raised for 4-10 weeks on semi-purified experimental diets containing 0mg, 2 mg, 8mg folate/kg diet. Folate concentrations were determined microbiologically using Lactobacillius casei (ATCC 7469). When compared to the folate adequate diet, the folate deficient diet decreased folate levels in plasma, liver and kidney , and the values were further decreased with experimental period. In rats reviving folate supplemented diets, plasma , liver and kidney folate adequate or supplemented diets, folate concentrations weer increased compared to animals on the folate adequate diet. In the folate adequate or supplemented diets, folate concentrations in the plasma and kidney were maintained at essentially the same level for 10 weeks . Folate concentrations in the liver, however, continued to increase with experimental period. Dietary folate intake seems to influence plasma and liver folate concentrations more than kidney folate concentrations. Folate excretions unrine and feces were significantly increased with dietary foalte intakes and experimental period. Folate excreted via urine was consideerably greater than that via feces. These resutls indicated that the foate supplemented diet improved plasma and tissue foalte status. Whether folate supplmentation improves foalte-dependent reactions remains to be researched.

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A Study on Distribution of Heavy Metals in Normal Korean Tissues: Lead, Cadmium and Copper Contents (한국인(韓國人)의 각장기조직중(各臟器組織中)의 미량중금속(微量重金屬) 원소분포(元素分布) : 연(鉛) 카드뮴 및 동(銅)의 함량(含量))

  • Jang, Seong-Gil;Moon, Bynug-Yul;Chung, Kyou-Chull
    • Journal of Preventive Medicine and Public Health
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    • v.15 no.1
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    • pp.95-110
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    • 1982
  • For the purpose of investigation to identify the quantities of heavy metals contained in the tissues of the Korean people, a series of analyses was conducted with atomic absorption spectrophotometry to measure the amount of lead, cadmium and copper distributed in various tissues, such as brain, liver, kidney, heart, lung, spleen, large intestine, hair, muscle, fat, stomach, costal cartilage, blood and urine, obtained from 30 cadaverous bodies who were believed not exposed to the said heavy metals during their life time either occupationally or therapeutically. 1. Lead content: Inter-individual difference was noted in lead contents in each tissue, vis., the average content of lead in hair was the highest with $14.90{\pm}9.74ppm$. The next was in costal cartilage that contained $5.56{\pm}3.86ppm$. The average contents of lead in liver, kidney and muscle were the lowest in value, showing $1.11{\pm}0.92ppm,\;0.73{\pm}0.48ppm\;and\;0.06{\pm}0.06ppm$, respectively, The lead contents in tissues of children under the age of 10 were significantly lower than those of adults, and the higher values were shown in males than in females in general. The lead contents in most of the tissues such as hair, costal cartilage, kidney, lung, fat, stomach, large intestine, heart, muscle and urine were well correlated with age. 2. Cadmium content: The average content of cadmium in kidney appeared to be the highest of other tissues showing $20.72{\pm}9.82ppm$, and liver came next with the value of $1.17{\pm}0.99ppm$. It was estimated that 83.9% of the total cadmium absorbed into the body was stored in kidney, 4.796 in liver, and the remaining 11.4% was distributed in the rest of the tissues. Cadmium contents in tissues showed difference between both sexes showing higher values in the females than in the males, which was quite contrary to the lead content. Cadmium contents in tissues steadily increased in amount with age, showing a significant correlation with age in all tissues. 3. Copper content: The average content of copper in hair was the highest with $10.36{\pm}2.21ppm$, and liver came next with $6.31{\pm}1.24ppm$. The copper that was absorbed into the body was distributed in each tissue: 29.9% in hair, 18.2% in liver, $5{\sim}11.0%$ in brain, heart and kidney, and $3.0{\sim}3.5%$ in stomach, blood, and lung. The copper contents in tissues of children under the age of 10 showed significantly higher values in liver, kidney, heart and large intestine than those of the adults. The copper contents in brain, costal cartilage and fat were well correlated with age showing the highest correlation coefficient of 0.870 (p<0.01) in brain tissue. There was no difference in copper contents in tissues between both sexes, and the values were, in general, lower than those for Japanese.

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Kinetic of Copper Accumulation and Elimination in Rockfish (Sebastes schlegeli) Tissues Exposed to Dietary Copper

  • Kim Seong-Gil;Jee Jung-Hoon;Kim Sang-Gyu;Kang Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.6 no.4
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    • pp.213-219
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    • 2003
  • Experiments were carried out to investigate the accumulation and elimination changes in the tissue of juvenile rockfish (Sebastes schlegeli) after sub-chronic dietary Cu (0, 50, 125, 250 and 500 mg/kg) exposure for 60 days and depuration for 30 days. The profile of Cu accumulation in the tissue of rockfish was dependent on the exposure periods and Cu concentration. Liver of rockfish is a more important storage tissue than other tissues, and the order of Cu accumulation in tissues was liver > intestine > kidney > gill > muscle. The accumulation factors were increased with the exposure period in gill, intestine, liver, kidney and muscle. An inverse relationship was observed between the accumulation factor and the exposure concentrations in the gill, kidney and muscle. Cu elimination in tissues of rockfish were decreased with periods for the 30 days of depuration except kidney and muscle. The order of Cu elimination in organs during depuration was intestine > liver > gill.

Studies on Effect of Dietary Zine on Tissue Trace Elements in the Rat (식이아연(食餌亞鉛)이 흰쥐의 조직중(組織中) 미양금속(微量金屬)에 미치는 영향(影響))

  • Suk, Young-Gun
    • Journal of Nutrition and Health
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    • v.5 no.2
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    • pp.91-103
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    • 1972
  • Zinc is one of the essential trace elements in the living organism for growth and health. The first identified metalloenzyme, carbonic anhydrase, is a zinc compound and several others have been described since. Among zinc deficiency syndromes in animals porcine parakeratosis has been successfully treated with zinc supplements, and in man a syndrome of anemia, hypogonadism, hepatosplenomegaly, and dwarfism, prevalent in parts of Iran and Egypt, has been ascribed to lack of zinc in the diet. Dietary zinc excess in the rat is manifested by a hypochromic, microcytic anemia, poor growth, reduction in liver catalase and cytochrome oxidase. The present study is an attempt to delineate the changes of tissue contents of trace elements, especially of iron, copper and zinc in liver and kidneys of the rats. Weanling albino rats, weighing 60 to 80gm. were used in this experiments. The rats were housed in cages with aluminum floors and received feed and distilled water ad libitum. Animals were divided into three groups, control, low zinc diet and high zinc diet groups. The high zinc diet group was subdivided into 0.5% Zn and 0.7%Zn groups. The supplementary copper or iron was added to the high dietary zinc groups. The animals were sacrificed and the tissues were washed several times with deionized water. The wet digested samples were analyzed by Hitachi Model 207 atomic absorption spectro-photometer for the determination of iron, copper and zinc in the liver and kidney. Hemoglobin level in the blood was measured by cyanmethemoglobin method. The results of this study are as follows: 1) All rats fed high zinc diets and low zinc diets gained less weight than control. Weight gain was not improved by the supplementary copper or iron and both. 2) Hemoglobin concentration was decreased significantly in the rats fed high zinc diets and less in the low zinc diet. Supplementary copper and iron to the higher zinc diet appeared to give some improvement of anemia. 3) The iron contents of the liver and kidneys were significantly decreased in the high zinc groups and the reduction was more significantly in the rats receiving higher zinc diet (0.7%). The supplementary copper caused a further depression of liver iron. On the other hand, the iron, added to the high zinc diet lessoned the severity of the decrease in liver iron and caused kidney iron to be maintained almost at the level found in the rats fed by zinc and supplementary copper diet. 4) High zinc diets did not change copper content of the liver and kidney. Supplementary copper elevated the concentration in the liver and kidney and added iron had no effect on the accumulation of copper in the liver and kidneys. 5) The high zinc diets caused marked increases of zinc content in the liver and kidney. Supplementary iron to the high zinc diet caused increases of zinc contents of liver and kidneys.

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Effects of Oenanthe javanica Extracts on Mercury Accumulation in Organs of the Mouse (미나리 추출물이 마우스의 장기내 수은 축적에 미치는 영향)

  • 조현욱;김명훈;황규영;민병운;박종철;김종홍
    • Toxicological Research
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    • v.15 no.1
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    • pp.1-8
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    • 1999
  • This study was performed to investigate the antitoxic effect of Oenanthe javanica extracts on orally administered mercury compound. Adult male ICR mice were exposed to methylmercuric chloride (CH3HgCl)through drinking water. The control, mercury treated and Oenanthe javanica treated groups not showed significant differences in mean body and organ weights of mice. The distribution of mercury in the cerebellum, kidney, liver and spleen of the mouse were examined according to a histochemical mathod. Grains of mercury traces were located in the purkinje cell and granular layers of the cerebellum and cortex of kidney respectively. Lesser staining of the grains was seen in the collecting tubules of medulla. in the liver, mercury accumulations were present primarily in the hepatocytes around portal area containing interlobular bile duct, artery and portal vein. Also grains of mercury traces were accumulated in the white pulp of the spleen. In the group of Oenanthe javanica extracts, staining intensity of mercury was decreased in the Purkinje cell layer of cerebellum and in the portal area of liver respectively. Staining patterns in kidney and spleen of extracts group were similar to that of only mercury treated group.

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Retrospective Study for Safety of Xie-xia Fa (瀉下法) by Euphorbia kansui Radix: Analysis of Clinical Features, Liver and Kidney Functions (감수(甘遂)를 이용한 사하법(瀉下法)의 안전성에 대한 후향적 연구: 임상반응, 간기능, 신기능을 중심으로 한 평가)

  • Lee, Seung-Wook;Kwon, Oh-Jun;Na, Hyun-Young;Ahn, Young-Min;Lee, Byung-Cheol;Ahn, Se-Young
    • The Journal of Internal Korean Medicine
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    • v.35 no.4
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    • pp.472-482
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    • 2014
  • Objectives: The purpose of this study was to report the safety of diarrhea-inducing treatment (Xie-xia Fa, 瀉下法) by Euphorbia kansui Radix, especially in the respect of clinical features, liver and kidney functions. Methods: Patients who were treated with Euphorbia kansui Radix powder between September 2013 and September 2014 were assessed retrospectively. Results: Less than 6 grams dosage of Euphorbia kansui Radix can induce several mild intestinal reactions but have no harmful effects on liver and kidney functions. Conclusions: Through this study, diarrhea-inducing treatment by less than 6 grams of Euphorbia kansui Radix can considered safe enough to be used in local clinics, but a cautious approach is needed.

A study on the ancient Five Viscera theory consisted of Liver, Heart, Stomach, Lung, Kidney (간심위폐신 오장 학설에 관한 고찰)

  • Park, Jaemin;Kim, Kiwang
    • Journal of Korean Medical classics
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    • v.28 no.1
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    • pp.195-206
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    • 2015
  • Objectives : Before Liver, Heart, Spleen, Lung, Kidney was widely accepted as Five Viscera, there had been several former types of Viscera system in pre-Western Han era. Above them, the Five Viscera system consisted of Liver, Heart, Stomach, Lung, Kidney was not yet hardly studied. So we tried to prove it's existence and tried to find it's historical context. Material and Methods : Via preceding research analysis, 6 books were selected as research material, and some other pre-Eastern Han dynasty classics were also analyzed. Results : We found direct evidence of above Five Viscera system in Zhuixingxun (墜形訓) in Huainanzi (淮南子), Wusemaizhen (五色脈診), and Western Han dynasty lacquer figure with meridian-points (excavated in Laoguanshan, Chengdu, China). Other clues showing existence of above Five Viscera system was also found in Yinyangshiyimaijiujing (陰陽十一脈灸經), Pianquecanggongliechuan (扁鵲倉公列傳). The rising of above Five Viscera system was related to physiological viewpoint transition of viscera in Western Han dynasty. Conclusions : In Western Han dynasty there was another Viscera System consisted of Liver, Heart, Stomach, Lung, Kidney.

Effect of Acute Heat Stress on Heat Shock Protein 70 and Its Corresponding mRNA Expression in the Heart, Liver, and Kidney of Broilers

  • Yu, Jimian;Bao, Endong
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.8
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    • pp.1116-1126
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    • 2008
  • The objective of this study was to investigate the expression and localization of heat shock protein 70 (Hsp70) and its mRNA in the heart, liver, and kidney of acutely heat-stressed broilers at various stressing times. Male AA broilers (n = 100) were randomly divided into 5 groups of 20 birds per group. After 30 d of adaptive feeding at ambient temperature, 80 experimental broilers were suddenly heat stressed by increasing the environmental temperature from $22{\pm}1^{\circ}C$ to $37{\pm}1^{\circ}C$. The 4 groups were heat stressed for 2, 3, 5, and 10 h, respectively. The localizations of Hsp70 protein and mRNA, determined by immunohistochemical staining and in situ hybridization, respectively, were demonstrated to be tissue dependent, implying that different tissues have differential sensibilities to heat stress. Intense Hsp70 staining was identified in the vascular endothelial cell of heart, liver and kidney, suggesting an association between expression of Hsp70 in vascular endothelial cell and functional recovery of blood vessels after heat shock treatment. Ante-mortem heat stress had a significant effect on the expression of Hsp70 protein and mRNA. The quantitation of Hsp70 protein and mRNA were both time and tissue dependent. During the exposure to heat stress, the heart, liver and kidney of broiler chickens exhibited increased amounts of Hsp70 protein and mRNA. The expression of hsp70 mRNA in the heart, liver and kidney of heat-stressed broilers increased significantly and attained the highest level after a 2-h exposure to elevated temperatures. However, significant elevations in Hsp70 protein occurred after 2, 5, and 3 h of heat stressing, respectively, indicating that the stress-induced responses vary among different tissues.

The Effect of Dietary Protein and Calcium Levels on the Cadmium Detoxication on Rats (식이 단백질과 Ca 수준이 흰쥐의 Cd 해독에 미치는 영향)

  • 권오란
    • Journal of the Korean Home Economics Association
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    • v.30 no.1
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    • pp.99-113
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    • 1992
  • This study was performed to investigate the effect of dietary protein and calcium levels on cadmium detoxication in rats. Seventy male Sprague-Dawley rats weighing 208 $\pm$ 19 g were blocked into 10 groups of 7 animals according to body weight. Five groups were fed 15% protein-0.6% calcium diet with 100ppm cadmium in drinking water for first 15days and the other 5groups fed same diet without cadmium in drinking water for same period and served as controls. After this 15-day intoxication period, each one of cadmium intoxication and control groups were fed each of 4 kinds of detoxifying diets different with protein(40%, 15%) and calcium(1.3%, 0.6%) levels without cadmimum in drinking water for following 15 days of detoxifying period. Results were summarized as follows: 1) Food intake, body weight gain, F.E.R. and weights of liver, kidney and femur were increased by detoxifying diets and high protein diet was most effective in weight gains of liver and kidney. 2) When cadmium and metallothionein contents of initial intoxication group and those of all detoxication groups were compared, cadmium and metallothionein contents in the liver were not changed, but those in kidney increased, and those in intestine decreased markedly. 3) Only dietary protein level affected cadmium and metallothionein distribution among organs, and cadmium contents of whole blood, liver, kidney and femur were lower in high protein diet, but metallothionein contents in liver and kidney were higher in high protein diet. 4) Gel filtration chromatogram showed that most of cadmium in the cytosol was bound to metallothionein fractions in high protein-high calcium group. Results obtained indicated that high protein diet was effective in cadmium detoxication by increasing the induction of metallothionein synthesis. But high calcium diet did not play a role in cadmium detoxication.

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Relationships Between Cadmium, Copper, Mercury, Zinc Levels and Metallothionein in the Liver and Kidney Cortex of Korean (한국인 간장 및 신장피질에 함유된 카드뮴, 구리, 수은, 아연 함량과 메탈로치오나인과의 관계)

  • Lee Sang Ki;Yoo Young Chan;Yun Yeo Pyo;Yang Ja YouL;Oh Seung Min;Chung Kyu Hyuck
    • Environmental Analysis Health and Toxicology
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    • v.19 no.4
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    • pp.383-388
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    • 2004
  • In order to elucidate the relationships between cadmium, copper, mercury, zinc levels and metallothionein in the liver and kidney cortex of Korean, the levels of Cd, Zn, Hg, Cu and metallothionein (MT) were determined in the kidney cortex and liver of 50 subjects deceased in the period of January-November, 2001 in the area of Seoul and Gyeonggi Province of Korea. The mean age of the population studied was 36.3+/-12.3 years. The tissues were digested with microwave digestion system and the elements were determined by inductively coupled plasma atomic emission spectrometry. MT was determined by the Cd-hemoglobin affinity assay. The determined levels (mean+/-SD) were: 33.9+/-18.9 micrograms Cd/g wet weight; 47.5+/-12.6 micrograms Zn/g wet weight; 2.5+/-0.57 microgram Cu/g wet weight; 0.26+/-0.31 micrograms Hg/g wet weight, 4.0+/-3.1 mg MT/g wet weight in renal cortex and 2.5+/-1.9 micrograms Cd/g wet weight; 46.9+/-15.0 micrograms Zn/g wet weight; 6.2+/-2.5 micrograms Cu/g wet weight; 0.10+/-0.15 micrograms Hg/g wet weight, 0.92+/-0.57 mg MT/g wet weight in the liver. Positive relationships between Cd and MT, sum of four divalent metal and MT in the kidney cortex were observed. No other correlation was found between Cu and MT, Hg and MT, Zn and MT in either organs.