• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1996.04a
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• pp.25-25
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• 1996

;Jindo Hongju is an unique red-colored traditional distilled wine of Korea. The unique attractive color of Jindo Hongju is due to the pigments of gromwell (Lithospermum erythrorhizon) root, derivatives of naphtoquinone such as shikonin and acetylshikonin. Which are extracted during the distillation process. The attractive color of the gromwell pigments is easily changed to dark red or to brown causing deterioration of the Quality of Jindo Hongju. Due to the discoloration of the pigments and to the limited supply of gromwell roots, some brewers manufacture spurious Jindo Hongju using artificial colorants. This study was performed to devise a simple method of detecting spurious Jindo Hongju products. The color of the gromwell pigments was greatly affected by pH change and the change could be demonstrated by the change of the absorption spectrum. At pH 4.0 the normal pH of Jindo Hongju, the absorption spectra of gromwell pigments and genuine Hongju products showed an absorption maximum of 520 nm. The absorption maximum was shifted to 570 nm and to 616 nm as the pH was raised to 7.0 and 11.0 respectively. This transition due to the pH change was also demonstrated on em chromaticity diagram. The characteristic transition due to pH change of gromwell pigment solution was not observed with an artificial colorant (red No.2) which was suspected to be used in the manufacture of imitation products. The absorption spectra of most of the Jindo Hongju collected from the market were similar to that of the gromwell pigments and showed the characteristic transition due to pH change with the addition of NaOH. However, with a few of the products, the absorption spectra was similar to that of the artificial colorant and the characteristic transition due to pH change was not observed, indicating these products might have been forged. The result of study suggests that the transition of the absorption spectrum and the change of the color due to pH change be used for the detection of imitation products. Farther more, since, at pH above 9.0, the color of the gromwell pigments and genuine Jindo Hongju could be visually differentiated from that of the artificial colorant and forged products, it might be possible that the forged products be easily detected by raising the pH to above 9.0 and visually comparing the color with that of the gromwell pigment at the same pH.me pH.

• Journal of the Korean Wood Science and Technology
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• v.35 no.3
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• pp.70-78
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• 2007

This study explored the efficient dyeing method of aged chestnut wood using the natural dyes extracted from Gardenia jasminoides for, grandiflora, Carthamus tinctorius L., Rhus javanica L., Lithospermum erythrorhizon S. et Z., Caesalpinia sappan L. and Castanea crenata S. et Z.. The color variation of the dyed chestnut woods was also quantitatively evaluated. The wood specimens revealed abundant colors through different dyeing conditions such as dyeing temperature, dye pH, soaking time, and brushing frequency. The chroma and stain concentration of colored woods were calculated with $L^*$, $a^*$ and $b^*$ in order to make color tone measurement. Brushing treatment for colored wood was confirmed as a more economical dyeing method than soaking treatment requiring more time when natural dyeing was done. Furthermore, the desirable tone and shade of color was easily obtained by repetitive brushing treatment using low color tone. During natural dyeing, good color expression was made with high temperature and acidic condition of a dyeing liquor.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.1
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• pp.91-96
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• 1992

Twenty kinds of medicinal herbs were extracted by water and 95% ethanol and then antimicrobial activity of the extracts was investigated against various kinds of microorganisms. Water extracts of Gardeniae fructus (Gardenia jasminoides), Lycii fructus (Lycium chinense) and Schizandrae fructus (Schizandra chinensis) showed inhibitory effects on the growth of most of the bacteria. In the case of ethanol extracts, the 3 kinds of the samples such as Gardeniae fructus, Schizandrae fructus and Lithospermi radix (Lithospermum erythrorhizon) showed inhibitory effects on the growth of almost all bacteria. In particular, ethanol extract from Phellodendri cortex (Phellodendron amurense) showed the best inhibitory effect on the growth of S. aureus in the concentration of 0.01%. By the way, inhibitory effects of water extracts from these medicinal herbs were not so good on the growth of fungi but those of ethanol extracts were better and ethanol extracts of Phellodendri cortex showed best. Antimicrobial activity was variable according to the used extracting solvent. For example, inhibitory effets of ethanol ext-racts were $2{\sim}100$ times better than those of water extracts. Ethanol extract of Lithospermi radix was the most effective not only bactericidal effects but also sensory evaluation tests for tastes.

• Korean Journal of Environment and Ecology
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• v.35 no.1
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• pp.1-23
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• 2021

This study conducted a survey on flora at Mt. susdolbon(508m), Mt. seongjubong (625.3m), and Mt. sulibong(596.2m), located in Yanggu-gun, Gangwon-do. A total of 403 taxa, including 6 forms, 43 varieties, 4 subspecies, 350 species, 255 genera, and 84 families, were surveyed in the three mountain areas. 18 taxa from 5 families of ferns, 3 taxa from 1 family of gymnosperms, and the dicotyledonous plants as 302 taxa from 69 families of the dicotyledonous plants were identified. The remarkable plants included 11 taxa of the Korean endemic plants, such as Populus tomentiglandulosa, Salix koriyanagi, Cimicifuga heracleifolia, Aconitum pseudolaeve, Clematis trichotoma, Echinosophora koreensis, Vicia chosenensis, Salvia chanryoenica, and Hemerocallis hakuunensis. The rare plants designated by the Korea Forest Service were 4 taxa, including Echinosophora koreensis as endangered species (EC), Cimicifuga heracleifolia, Epimedium koreanum, and Lilium cernuum. The Least Concern species (LC) were 13 taxa, including Persicaria orientalis, Exochorda serratifolia, Viola diamantiaca, Lithospermum erythrorhizon, and Trigonotis icumae. The invasive alien plants were 28 taxa, including Persicaria orientalis, Rumex crispus, Phytolacca americana, Cerastium glomeratum, Chenopodium album, Chenopodium ficifolium, Amorpha fruticosa, Robinia pseudoacacia, Trifolium pratense, Trifolium repens, Oxalis dillenii, Euphorbia supina, and Oenothera biennis, accounting for 6.9 percent of the 403 taxa of the surveyed plants.

• Journal of the Korean Wood Science and Technology
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• v.32 no.5
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• pp.75-85
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• 2004

The natural dyes from Gardenia jasminoides, Carthamus tinctorius L., Rhus javanica, Lithospermum erythrorhizon, Caesalpinia sappan L., and Castanea crenata were extracted under different pH in distilled water, As the pH in distilled water went from acid to alkali, the much deeper colors in the same color tone were generated from the individual plant species. Before dyeing, wood species were treated by different mordants including AI, Cu, Cr and Fe for color-fixing between wood and the natural dyes. Each mordant could develop independent color on the surface of the woods. The wood species dyed by the natural dyes created deep-tone colors according to higher pH and temperature of the dyeing solution, leading to deeper penetration of the dyes into the wood tissues. Finally through the computer modelling of natural-dyed wood furniture, it was confirmed that the colored furniture can adequately be compatible with the current interior spaces of diverse colors.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.30-55
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• 2022

This study conducted a flora survey in the areas of Mt. Sambong (282m) and Mt. Jaung (263m), located in Paju-si, Gyeonggi-do. A total of 434 taxa, including 6 forms, 34 varieties, 11 subspecies, 383 species, 271 genera, and 94 families, were surveyed in the two mountain areas. The ferns were identified as 24 taxa from 10 families, the gymnosperms as 8 taxa from 2 families, the dicotyledonous plants as 322 taxa from 73 families, and the monocotyledonous plants as 80 taxa from 9 families. Five taxa, including Salix koriyanagi, Cimicifuga heracleifolia, Forsythia koreana, Paulownia coreana, and Hemerocallis hakuunensi, were identified as the Korean endemic plants. Eight taxa, including Cimicifuga heracleifolia, Tylophora floribunda, and Iris minutiaurea as Vulnerable species, andAristolochia contorta, Viola diamantiaca, Lithospermum erythrorhizon, Lilium distichum, andArisaema heterophyllumas Least Concern species, were designated as rare and endangered plants designated by the Korea Forest Service. The invasive alien plants were 40 taxa, including Rumex crispus, Phytolacca americana, Cerastium glomeratum, Silene armeria, Chenopodium album, Chenopodium ficifolium, Magnolia obovata, Potentilla supina, Amorpha fruticosa, Medicago sativa, Robinia pseudoacacia, Trifolium repens, Euphorbia supina, Hibiscus trionum, and Oenothera biennisaccounting for 9.2 percent of the 434 taxa of the surveyed plants. As this area is a mountainous area close to settlement facilities, it is believed that ecological preservation measures and measures of specialized plants should be implemented.

• Journal of Life Science
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• v.19 no.11
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• pp.1637-1643
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• 2009

Shikonin, a component of Lithospermum erythrorhizon Sieb. et Zucc, exerts various characteristics such as anti-inflammatory, anti-cancer and anti-obesity functions. To elucidate the molecular mechanism of shikonin-induced inhibition of adipogenesis, we analyzed the mRNA expression level of various adipogenesis-related factors including C/EBPs (CCAAT/enhancerbinding proteins) and $PPAR{\gamma}$ (peroxisome proliferator-activated receptor $\gamma$). The data showed that mRNA expressions of C/$EBP{\beta}$ and C/$EPB{\delta}$ were only slightly changed by shikonin treatment, but mRNA expressions of $PPAR{\gamma}$ and C/$EPB{\alpha}$ were significantly down-regulated. Then, we tested whether upstream regulators of C/$EBP{\beta}$ and $PPAR{\gamma}$ were involved in anti-adipogenesis of shikonin. C/$EBP{\gamma}$ and CHOP (C/EBP homologous protein), which are upstream regulators of C/$EBP{\beta}$, were not affected by shikonin treatment. On the contrary, the mRNA level of KROX20 was markedly down-regulated by shikonin treatment. These results suggest that KROX20 might regulate downstream factors of adipogenesis through C/$EBP{\beta}$-independent pathway. The expression of KLF15 (Kruppel-like factor15), which is a member of KLF family and is a upstream regulator of $PPAR{\gamma}$, was dramatically decreased by shikonin treatment, but KLF2 was not changed. Shikonin had no impact on the expression of KLF5 in the early stage of adipogenesis, but shikonin increased expression of KLF5 in the late stage of adipogenesis. Even though mRNA expression of KLF5 was moderately changed by shikonin treatment, its effect may be small compared with the effect of KLF15, which was markedly inhibited. Taken together, these results suggest that shikonin inhibits adipogenesis through the down-regulation of $PPAR{\gamma}$ and C/$EPB{\alpha}$, which is mediated by the down-regulation of two pro-adipogenic factors, KROX20 and KLF15.