• 생약학회지
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• 제34권2호통권133호
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• pp.166-171
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• 2003

The hepatoprotective effects of Liriopis tuber on carbon tetrachloride $(CCl_4)$ intoxicated rats were studied. The water extract prepared from Liriopis tuber were administrated to the pretreatment group orally once a day for successive 7 days, followed by treatment with $CCl_4$ on the seventh day. The post treatment group was treated with $CCl_4$ and then the water extract was administrated. The activities of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactate dehydrogenase (LDH), and contents of total cholesterol and triglyceride in pretreatment rats serum were significantly decreased compared to the only $CCl_4$ treated rats. The level of high density lipoprotein (HDL) cholesterol was considerably increased compared to the only $CCl_4$ treated rats. In the pretreatment group with the water extract of Liriopis tuber tissue destruction was not observed in light microscopic investigation, compared to the only $CCl_4$ treated rats. These results suggest that Liriopis tuber have potent hepatoprotective effect against carbon tetrachloride intoxicated rats.

• 대한본초학회지
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• 제28권2호
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• pp.33-38
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• 2013

Objects : Apoptosis leads to the death of a cell. The mitochondrial pathway of apoptosis, a process regulated by the Bcl-2 family of proteins, plays a key role in various biological processes. The tuber of Liriope platyphylla Wang et TANG (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic, antitussive, expectorant and anti-asthmatic properties. The purpose of this study was to observe the effect of the Liriopis tuber on mitochondrial-mediated apoptosis in PC-12 cells. Method : A cytotoxic test on Liriopis Tuber water extract was conducted and another MTT assay was conducted to observe the cytoprotective effect against 4-HNE 25 ${\mu}M$ that causes oxidative stress in PC-12 cells for 24 hours. In addition, in order to observe the expression of Bcl-2 and Bax protein involved with apoptosis, western blot was conducted. Results : The LT water extract had no toxicity for PC-12 cell. In the cytoprotective effect against 4-HNE, both of the group treated with 50 ${\mu}g/m{\ell}$ and 100 ${\mu}g/m{\ell}$ of LT water extract showed a significant increase in comparison with the control group. In Bax protein expression, all the experimental groups treated with LT water extract showed a decrease in comparison with the control group but had no significance. In Bcl-2 protein expression, all the experimental groups treated with LT water extract showed a significant increase in comparison with the control group. Conclusion : These results suggest that LT is effective in reducing apoptosis.

• 생약학회지
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• 제34권1호통권132호
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• pp.65-69
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• 2003

This study was performed to examine the effects of Liriopis Tuber extracts to high cholesterol diet on lipid metabolism in rats. Male Sprague-Dawley rats of $90{\pm}10\;g$ were divided into six groups; basal diet (normal group), 1% high cholesterol diet (control group), high cholesterol diet supplemented with 3%, 5% water extracts (C3W, C5W group) and high cholesterol diet supplemented with 3%, 5% MeOH extracts (C3M, C5M group). These experimental diets were fed for 4 weeks. The lipid contents on serum, liver, feces were lower Lriopis Tuber extracts groups than control group. Especially, the effects of water extract was better than MeOH extract.

• 한국식품영양과학회지
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• 제30권1호
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• pp.20-24
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• 2001

This study was conducted to analyze the general components and to investigate sensory evaluation of hot water extract prepared by boiling at 10$0^{\circ}C$ for 3 hr with 4-fold water. The contents of total soluble soild, non-reducing sugar, total saponin, ash and total protein were 15.95%, 6.54%, 1.735, 0.33% and 0.40%, respectively. The contents of succinic, malic and acetic acid in the extracts were 111.48 mg%, 23.67mg% and 18.36mg%, respectively. The major free amino acids and minerlas of the extract were hydroxyproline 1,290.0 $\mu\textrm{g}$%, glutamic acid 456.2$\mu\textrm{g}$% and potassium 151.35 mg%, respectively. Bitter taste in the extract was not observed, whereas sweet, sour and astringent taste were observed.

• 대한한방내과학회지
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• 제25권4호
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• pp.93-104
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• 2004

Objectives : The aim was to identify the inhibitory effects of Liriopis Tuber on bleomycin-induced lung fibrosis by analysing the changes of imflammatory cell cytokines and SHI(Semiquantitative Histological Index), Materials and Methods : In this study fibrosis prone C57BL/6J mice were used. Control group was treated with blomycin(0.06mg/0.1 ml) by IT(intratracheal) instillation which is a popular method of inducing lung fibrosis and sample group took Liriopis Tuber water extract(38.0mg/10g body weight) orally for 14 days after IT instillation of blomycin. We measured the total and differential count of WBC, $IFN-{\gamma}$ & IL-4 in mice BALF and SHI(Semiquantitative Histological Index) from lung tissues of mice. BALF and lung tissues of mice were taken 14 days after IT instillation of blomycin. Results : In sample group total WBC count, proportion of neutrophil, SHI and IL-4 significantly(p<0.05) decreased, proportion of macrophage significantly(p<0.05) increased and proportion of lymphocyte, $IFN-{\gamma}$ did not decrease significantly. Conclusions : This study suggests that Liriopis Tuber has an inhibitory effects of pulmonary fibrosis by attenuation of inflammation and Th2 immune response. To determine whether this herbal medicine contribute to cure and prophylaxis of pulmonary fibrosis, further studies on the role of $IFN-{\gamma}$ relating to fibrosis are required.

• 대한한방부인과학회지
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• 제34권3호
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• pp.15-28
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• 2021

Objectives: This study was designed to examine the anti-cancer activity by innate immunomodulating and anti-inflammatory effects of liriopis tuber water extract (LPE). Methods: Cell cytotoxicity was tested with 4T1 mouse mammary carcinoma cells, spleen cells, macrophage, and RAW264.7 cells. To investigate innate immunomodulating effects of LPE on macrophage, we measured tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). To investigate innate immunomodulating effects of LPE on RAW264.7 cell, we measured TNF-α, interleukin-6 (IL-6). In addition, TNF-α and nitric oxide (NO) induced by lipopolysaccharide (LPS) were measured after treating with LPE to observe innate immunomodulating effect of LPE on RAW264.7 cell. Also, mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis. Results: In an in vitro cytotoxicity analysis, LPE affected tumor cell growth above specific concentration. As compared with the control group, the production of TNF-α, IL-12, and IL-10 were increased in macrophage. As compared with the control group, the production of TNF-α and IL-6 were increased in RAW 264.7 cell. The expression of TNF-α and NO induced by LPS after treating LPE was decreased. In addition, treatment of RAW 264.7 cell with LPE increased the phosphorylation levels of p-extracellular signal-regulated kinase (p-ERK), p-Jun N-terminal kinase (p-JNK), and p-p38. Conclusions: LPE might have impact on the anti-cancer effect by activation of innate immune system and inflammation control.

• 대한본초학회지
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• 제35권4호
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• pp.9-16
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• 2020

Objective : To identify the effects of the water extract of Liriope platyphylla tuber (Liriopis tuber, LT) on the activation of astocytes, we investigated the regulatory effects of LT extract on H₂O₂-induced oxidative damage in C6 rat astrocytes. Methods : LT extract was extracted with boiling water. C6 cell line were treated with LT extract at 1, 2, and 3 mg/㎖ or without for 30 min and then stimulated with H₂O₂ at 5 ㎛ for 24 hr. The cell viability was measured by MTT assay. The expression of glial fibrillary acidic protein (GFAP), signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (pSTAT3), cyclooxygenase (COX-2), Nuclear factor-κB (NF-κB), superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1), catalase, Akt, phospho-Akt (p-Akt) phosphoinositide 3-kinases (PI3K), and protein kinase C alpha (PKCα) proteins were determined by Western blot, respectively. GFAP expression was also observed with immunocytochemistry under a fluorescence microscope. Results : LT extract induced cell proliferation in H₂O₂-stimulated C6 cells. LT extract significantly inhibited the expression of GFAP, NF-κB and COX-2 and increased the expression of HO-1 and the phosphorylation of STAT3 in H₂O₂-stimulated C6 cells. LT extract also significantly increased the phosphorylation of Akt and decreased the expression of PKCα in a dose-dependent manner in H₂O₂-stimulated C6 cells. Conclusions : LT extract can regulate H₂O₂-induced activation of astrocytes through inhibiting the expression of NF-κB, COX-2 and regulating Akt / HO-1, STAT3 or PKCα signaling pathway.

• 한국식품저장유통학회지
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• 제11권2호
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• pp.154-159
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• 2004

캔음료용 건강미숫가루를 개발하기 위하여 쌀, 보리 및 콩을 이용하여 기본미숫가루용액을 만든 후 몇 종의 한약재를 선발하여 적정 혼합비율로 혼합 추출, 첨가하여 건강미숫가루용액을 제조하였다. 이때 기본미숫가루용액으로 적당한 재료의 혼합은 물 100mL에 쌀, 보리 및 콩을 각각 5 g씩 혼합한 것이 가장 물성이 좋았다. 건강미숫가루용액 제조를 위하여 첨가한 한약재의 추출물은 맥문동 50, 오미자 15, 인삼 10, 대추 25의 비율로 혼합 후 10배 량의 물을 가하여 75$^{\circ}C$에서 36시간 처리하여 얻었으며 기본미숫가루용액 80에 혼합한 한약재 추출물 20의 비율로 혼합한 것이 기본미숫가루용액보다 기호도가 높았다. 건강미숫가루용액의 장기 저장을 위하여 가압살균처리를 함으로써 물성이 걸쭉한 형태로 변하므로서 살균처리시의 건강미숫가루는 배합비율을 기본미숫가루 60, 한약재혼합추출물 30, 물 10의 비율로 달리할 필요가 있었으나 기호도는 살균처리하지 않은 것보다 낮은 경향이었다. 살균처리하지 않은 건강미숫가루용액은 첨가한 한약재추출물에 의하여 방부 효과를 나타내었으며 살균처리하지 않은 상태로 제품화할 경우 $25^{\circ}C$에서 6일간 저장이 가능하였다.

• 한국식품영양과학회지
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• 제46권2호
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• pp.229-236
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• 2017

본 연구에서는 증숙 맥문동을 첨가한 양갱 제조조건을 반응표면분석법을 이용하여 최적화하여 저칼로리 양갱의 개발 가능성을 검토하고자 하였다. 증숙 맥문동 열수 추출물, 설탕 및 한천의 함량을 독립변수로 하여 16가지의 양갱을 제조하여 pH, 수분 함량, 당도, 색도 및 갈변도 등의 이화학적 품질 특성과 조직감, 관능평가를 수행하였다. 이화학적 품질 특성 중에서 pH 및 갈변도는 증숙 맥문동 열수 추출액, 당도 및 수분 함량은 설탕, 색도는 증숙 맥문동 열수 추출액과 설탕의 양이 품질 특성에 더 많은 영향을 나타냈으며, 조직감에 있어서 씹힘성과 검성은 분말한천의 양에 의존하는 경향을 나타내었다. 또한, 관능평가에 있어서 유의성을 나타낸 외관, 색, 단맛, 단단함, 질감 및 종합적인 기호도의 경우 증숙 맥문동 열수 추출물, 설탕이 증가할수록 기호도가 증가하는 것으로 나타났다. 이러한 결과를 바탕으로 당류의 사용은 최소화하면서 최적의 양갱의 품질 특성 및 기호도를 나타내는 조건은 증숙 맥문동 열수 추출물이 35 mL, 설탕은 55.23 g, 분말한천은 3.39 g으로 최적 첨가량이 예측되었으며, 기본 배합비와 비교한 결과 단맛은 유의적 차이가 나타나지 않고 그 외의 모든 관능평가 항목에서 유의적으로 우수하였다. 따라서 증숙 맥문동을 식품에 활용할 경우 다양한 저칼로리 제품의 개발에 이용이 가능할 것으로 판단된다.

• 약학회지
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• 제36권2호
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• pp.140-149
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• 1992

Anti-allergic action of each water extracts of some crude drugs was investigated in mice and rats. The activity of hyaluronidase which was used in the screening test of anti-allergic action was inhibited significantly by Amomi Semen, Asiasari Radix, Cimicifugae Rhizoma, Cinnamomi Ramulus, Glycyrrhizae Radix and Scutellariae Radix. The 48-hour homologous passive cutaneous anaphylaxis(48-hr PCA) in mouse ear was inhibited significantly by intraperitoneal(i.p.) injection of Amomi Semen, Cimicifugae Rhizoma, and ketotifen, a comparative drug of an anti-allergic action. The increase of vascular permeability induced by histamine or serotonin was inhibited significantly by i.p. injection of Amomi Semen, Cimicifugae Rhizoma, Cinnamomi Ramulus and ketotifen. In rat dorsal skin, the increase of vascular permeability which was induced by histamine, serotonin or compound 48/80 was inhibited significantly by i.p. injection of Amomi Semen, Asiasari Radix, Cimicifugae Rhizoma, Scutellariae Radix and ketotifen. Armeniacae Semen and Liriopis Tuber which had not inhibited hyaluronidase activity did not inhibit 48-hr PCA and the increase of histamine, serotonin or compound 48/80-induced vascular permeability in mice and rats. These results suggest that each water extract of Amomi Semen and Cimicifugae Rhizoma has anti-allergic action.