• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.461-465
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• 1998

The suspension culture of an anchorage-dependent cell line (Bok-l) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth ($6.0\times10^6cells/m\ell$) and viability (>80%) at 10 rpm were higher than that at 0 rpm ($1.9\times10^6cells/m\ell$, 65~80%) and 30 rpm ($1.8\times10^6cells/m\ell$ 40~76%). The size of cells became smaller at the agitation rate of 30 rpm. The antibacterial activities of cell extracts from suspension cultured cells were confirmed against gram-negative and gram-positive bacteria by the inhibition zone assay and the liquid growth inhibition assay.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2142-2147
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• 2007

Experimental study was conducted to evaluate the performance of a miniature loop heat pipe (MLHP) with non-inverted meniscus type capillary structure. All parts of MLHP in this study were made of copper including the capillary structure and the distilled water was used as a working fluid of MLHP. The outer diameter of evaporator was 9 mm and its length was 119 mm. The effective pore size of the capillary structure was 30 micron and its porosity was 60%. The vapor transport line, the liquid transport line and the condenser were consisted of single 4.0 mm copper tube. The distance between the evaporator and the condenser region was 200 mm and the length of the loop was 969 mm. This MLHP was operated successfully at any orientation but the gravity highly influenced the thermal performance of the MLHP. The maximum thermal load was 130 watts at the bottom heat mode and the 20 watts at the top heat mode.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.7 no.2
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• pp.234-248
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• 1995

A super-heat pump system composed of a suction line heat exchanger, low and high stage economizers, and a screw compressor is simulated to examine the energy performance and design options. CFC12, HCFC22, HFC134a, HCFC22/HCFC142b, HFC32/HFC134a, and HFC125/HFC134a are used as working fluids for comparison. The results indicate that the proposed system charged with appropriate mixtures is up to 33.4% more energy efficient than the normal system with CFC12. The performance of the super-heat pump system charged with mixtures was influenced by such factors as the temperature matching, heat source temperature difference, low stage economizer, and high stage economizer. The fluids with a larger liquid specific heat such as HFC134a would have more benefits when a suction line heat exchanger is installed. 40%HCFC22/60%HCFC142b mixture seems to be a good candidate to replace CFC12. On the other hand, 25%HFC32/75% HFC134a would be a good long term candidate to replace HCFC22.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1617-1622
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• 2004

Two-phase flow of liquid and gas through pipe lines are frequently encountered in nuclear power plant or industrial facility. Pressure waves which can be generated by a valve operation or any other cause in pipe lines propagate through the two-phase flow, often leading to severe noise and vibration problems or fatigue failure of pipe line system. It is of practical importance to predict the propagation characteristics of the pressure waves for the safety design for the pipe line. In the present study, a theoretical analysis is performed to understand the propagation characteristics of a weak shock wave in a bubbly flow. A wave equation is developed using a small perturbation method to analyze the weak shock wave through a bubbly flow with comparably low void fractions. It is known that the elasticity of pipe and void fraction significantly affect the propagation speed of shock wave, but the frequency of relaxation oscillation which is generated behind the shock wave is not strongly influenced by the elasticity of pipe. The present analytical results are in close agreement with existing experimental data.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.3122-3122
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• 2001

Documented quality control plays a vital role I the production of technical “Depth filter” sheets used in industries such as Beverage and pharmaceutical. Depth filter sheets which can be up to several millimeters thick are stacker in plate and frame filter systems. They are the core of stainless steel filter systems which can be up to several meters high. FT-NIR Spectroscopy has many potential applications in the whole production line of filter sheets. Raw materials such as different types of cellulose pads, white powdery fillers (e.g. Kieelgur, Perlite) or liquid chemicals such as wet-strength agents we, with the help of NIR, easy to identify. NIR can also determine physical parameters such as particle size, essential for the filtration behavior. FT-NIR can be used for the quality parameters of the final product. Moisture and permeability can be determined, and with the help of the speed of this NIR method it is possible to correct possible faults quickly in the production process. Waste production can be minimized which is good for both the product profitability and the environment. Further tests have shown that it is also possible to use NIR on-line in the production area, to check the concentrations and the homogeneity of the paper suspension consisting of cellulose fibres, fillers and additives.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1055-1060
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• 2001

Experimental data for the on-line estimation of cell concentration and growth rate are presented. For this purpose, we utilized the on-line calculation of the oxygen uptake rate (OUR), which was derived from a liquid phase dynamic mass balance for the oxygen during the active growth phase in cyclosporin A (CyA) fermentation. The cell yield coefficient, based on the oxygen $(Y_{x/o})$for both suspended and immobilized cells of Tolypocladium inflatum, was estimated as $1.9 gDCW/gO_2$ from a very good linear correlation between the cell mass produced and the total oxygen consumed. The calculated yield showed a good agreement with the value of $(Y_{x/o})$ generated from the correlation between the cell growth rate and the oxygen uptake rate. In addition, further experimental data are given, which were also applied to determine the specific oxygen uptake rate of T. inflatum cells during the exponential phase of CyA fermentation. A theoretical basis for the analysis of these fermentation parameters is also provided.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1995.04a
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• pp.534-544
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• 1995

Many electronic products employ menu-driven interfaces for user-system dialogue. Unlike the software user interfaces, a small single-line display, such as a Liquid Crystal Display, is typically used to present menu items. Since the display can show only a single menu item at a time, more serious navigation problems are expected with single-line display menus(SDM). This study attempts to provide a set of unique guidelines for the design of the SDM based on empirical results. A human factors experiment was conducted to examine the effects of four design variables: menu structure, user experience, navigation aid, and number of targets. The usability of design alternatives was measured quantitatively in four different aspects, which were speed, accuracy, inefficiency of navigation, and subjective user preference. The analysis of variance was used to test the statistical effects of the design variables and their interaction effects. A set of design guidelines was drawn from the results which can be applied to the design of human-system interfaces of a wide variety of electronic consumer products using such displays. Since more generalized guidelines could be provided by constructing prediction models based on the empirical data, some powerful performance models are also required for the SDM. As a preliminary study, a survey was done on the performance models for ordinary computer menus.

• 한국전산유체공학회:학술대회논문집
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• 2010.05a
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• pp.326-334
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• 2010

In this study, we provide a comprehensive review of the CIP(Constrained Interpolation Pro file/Cubic Interpolated Propagation) method with a pressure-based algorithm that is known as a general numerical solver for soled liquid, gas and plasmas. And also we introduce a body-fitted grid system(Soroban grid) for computation of strongly nonlinear marine hydrodynamic problems such as slamming water on deck, wave impact by green water. This grid system can keep the third-order accuracy in time and space with the help of the CIP method. The grid system consists of the straight lines and grid points. In the 2-dimensional grid case, each grid points moving in these lines like abacus - Soroban in Japanese. The length of each line can be different and the number of grid points in each line can be different. Mesh generation and searching of upstream departure point are very simple and possible to mesh-free treatment. To optimize computation of free-surface and multi-fluid flows, We adopt the C-CUP method. In most of the earlier computations, the C-CUP method was used with a staggered-grid approach. Here, because of the mesh free nature of the Soroban grid, we use the C-CUP method with a collocated-grid approach.

• Journal of Energy Engineering
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• v.12 no.2
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• pp.101-108
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• 2003

The purpose of this study is to develop an entrainment model of the Pressurized Water Reactor (PWR) U-tube Steam Generator (SG) for Main Steam Line Break (MSLB) analyses. Generally, the temperature of the inside containment vessel at MSLB is decreased by introducing the liquid entrainment effect. This effect makes a profit on the aspect of integrity evaluation for Equipment Environmental Qualification (EEQ) in the containment. However, the target plant, Kori unit 1 does not have the entrainment data. Therefore, this study has been performed. RETRAN-3D and LOFTRAN computer programs are used for the model development. There are several parameters that are used for the initial benchmark, such as Combustion Engineerings (CE) experimental data and the RETRAN-3D model which describes the test leg. A sensitivity study is then performed with this model in which the model parameters are varied until the calculated results provide reasonable agreement with the measured results for the entire test set. Finally, a multiplication factor has been obtained from the 95/95 values of the calculated (best-estimate) quality data relative to the measured quality data. With this new methodology, an additional temperature margin of about 40$^{\circ}C$ can be obtained. So, the new methodology is found to have an explicit advantage to EQ analyses.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.17 no.1
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• pp.29-34
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• 1981

More recently, unsteady flow in small-diameter pipes plays a major role in liquid propellantrocket systems, hydraulic and pneumatic control system, and elsewhere. And it has shown that line dynamics can have a marked effect on the hydraulic system characteristics. In this paper, transfer function of hydraulic lines with an accumulator and an outlet orifice is' developed and compared with experimental data from frequency response tests at various airvolume(V.) and the location of accumulator(ld1t), so that their performance may be correctly and easily predicted and the design of the systems incorporating them improved. The obtained results are as follows: 1. The dynamic response of hydraulic lines may be analyzed more accurately by use of the viscous term(22) in unsteady laminar flow. 2. There was good agreement between the theoretical and experimental results of this investigation, and hydraulic systems with liines included an accumulator can be analyzed more accurately by use of the pressure transfer function given by eq. (16). 3. For the mitigation of surge in hydraulic lines, it is more effective that the location ofaccumulator is close to the pipe outlet side. 4. According to the gas volume of accumulator is increased(the sealing pressure is close tomean line pressure), the damping effect of pressure wave is improved.