• 제목, 요약, 키워드: Liquid Semen

검색결과 106건 처리시간 0.046초

돼지 액상정액의 보존액, 보존온도 및 기간이 정액성상과 번식성적에 미치는 영향 (Effect of Extender, Preservation Temperature and Period of liquid Boar Semen on Semen Characteristics and Reproductive Performance)

  • 김인철;이장희;김현종;박창식
    • 한국가축번식학회지
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    • v.26 no.1
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    • pp.9-16
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    • 2002
  • 돼지 인공수정센터에서 사육중인 인공수정용 종모돈을 이용하여 1995년부터 2000년까지 보존액 종류, 보존온도 및 보존기간에 따른 정액성상 변화와 번식성적을 조사하여 돼지 인공수정시 번식성적 향상과 실용화에 기여코자 본 연구를 실시하였다. 1. 돼지 액상정액의 보존액 종류에 따른 보존기간별 활력은 Androhep 보존액은 3일째부터, BTS 및 Modena는 5일째부터 현저하게 감소하는 경향을 보였고 (P<0.05), pH변화는 6.24~7.06 범위에서 변이가 심하게 관찰되었으며 Androhep 보존액이 산도가 낮은 경향을 보였으나 전반적으로 규칙 적 인 경향을 나타내었다. 보존액 종류별 보존기간에 따른 분만율은 BTS, Modena 및 Androhep 보존액 모두 5일 보존까지 차이가 없었다. 보존액별 분만율은 Androhep 보존액이 BTS 보존액과는 차이가 없었으나 1일 보존과 5일 보존에서 Modena보존액 보다 우수하였다 (P<0.05). 산자수는 보존 기간별 보존액간에 차이는 없었으나 Androhep 보존액은 3일 보존부터 산자수가 유의적으로 감소하였다 (P<0.05). 2. 보존온도에 따른 액상정액의 운동성과 정상첨체 비율에서 17$^{\circ}C$ 보존정액의 운동성은 3일째부터, 정상 첨체율은 4일째부터 감소하였으나 5$^{\circ}C$ 보존정액은 4일 보존까지 정액성상의 변화가 크지 않은 것으로 나타났다. 17$^{\circ}C$에 보존한 액상정액이 5$^{\circ}C$에 보존한 것보다 분만율은 현저히 높았으나 산자수는 비슷한 경향을 보였고, 분만율은 보존 2일째부터 산자수는 3일째부터 감소하였다. 5$^{\circ}C$ 보존정액은 4일까지 분만율에 큰 변화가 없었으나 산자수는 다소 감소하는 경향을 보였다.

액상정액을 이용한 인공수정시 품종, 계절, 인공수정 횟수 및 정자농도가 번식성적에 미치는 영향 (Effects of Breeds, Insemination Time, Breeding Season, Sperm Concentration on Reproductive Performance of Sows Inseminated by Liquid Boar Semen)

  • 김인철;박창식;이규승;서길웅;한성욱
    • 농업과학연구
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    • v.28 no.2
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    • pp.85-91
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    • 2001
  • 돼지에서 액상정액을 이용한 인공수정이 번식성적에 미치는 영향을 구명하기 위하여 축산기술연구소 종축개량부 (충남, 성환) 돼지 인공수정센터에서 사육중인 인공수정용 종모돈을 이용하여 1995년부터 2000년까지 액상정액을 이용한 인공수정시 종모돈의 품종, 인공수정 횟수, 계절 및 정자농도가 번식성적에 마치는 영향을 조사하여 돼지 인공수정시 번식성적 향상과 실용화에 기여하고자 본 연구를 실시하였다. 액상정액 인공수정시 정액을 생산한 종모돈의 품종 (Landrace, Yorkshire, Duroc)간 번식성적은 유의차가 인정되지 않았다. 발정당 2회 또는 3회 인공수정 시에도 번식성적에 차이가 없었다. 인공수정을 실시한 계절별로 번식성적을 조사하였으나 계절간 차이점은 발견할 수 없었다. 액상정액 인공수정시 1회 주입정자의 최저농도를 구명코자 활력 70% 이상인 정자수를 기준으로 80 ml 병당 정자농도를 3.0, 2.5, 2.0 및 $1.5{\times}10^9$로 조절하여 인공수정한 결과 수태 율, 분만 율, 총산자수 및 생존산자수에서 통계적인 유의차가 인정되지 않아 1회 주입정자농도는 운동성 정자 기준으로 $1.5{\times}10^9/80ml$ 이상으로 조절하면 인공수정에 실용적으로 사용할 수 있음을 보였다.

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Effects of Different Concentrations of Escherichia coli and Days of Preservation on Boar Sperm Quality

  • Chung, Ki-Hwa;Kim, In-Cheul;Son, Jung-Ho
    • Reproductive and Developmental Biology
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    • v.37 no.4
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    • pp.213-217
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    • 2013
  • The objective of this study was to investigate the effect of bacterial contamination on elapsed time after preservation on boar semen. Known numbers of Escherichia coli (E. coli) were inoculated to freshly ejaculated semen and sperm parameters such as viability, motility, agglutination, acrosome integrity and hypo-osmotic swelling test were performed during 7 days of liquid preservation. Semen samples were prepared using antibiotic free BTS extender and 4 different levels of E. coli were treated to semen with following concentrations; 3,000, 5,000, 7,000, 10,000 CFU/ml of sperms. Semen samples were preserved at $17^{\circ}C$ for 7 days in semen storage until analyzed. Aliquots were subjected to measure the sperm viability, motility and agglutination using computer assisted sperm analysis (CASA) system, acrosome integrity was performed using chlortetracycline (CTC) staining method and hypo-osmotic swelling test was performed using hypotonic solution from day 1 (day of semen collection) to 7. Detrimental effects on sperm motility and viability were observed 3 days after preservation at the level of 5,000 CFU/ml (p<0.05). Percentage of sperm abnormality was higher (p<0.05) in over 5,000 CFU/ml groups. Sperm agglutination rate was also significantly higher (p<0.05) in groups of 5,000 and 7,000 CFU/ml. The rate of acrosome reacted sperm was higher as preservation time goes in all the samples but the pattern was clearly higher among E. coli contaminated groups (p<0.05). The sperm membrane integrity in terms of hypo-osmotic test, E. coli affects little compared to other sperm parameters. The deleterious effects observed due to the bacterial contamination in semen suggest that importance of hygiene protocol to minimize the bacterial contamination during semen collection and processing.

돼지의 자궁내 인공수정기술개발에 관한 연구 (Development of Intrauterine Insemination Technique in Pig)

  • 공일근;정금택;이정우;정수룡;오인석;유대중;이효상;김기수;배인휴
    • 한국수정란이식학회지
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    • v.17 no.1
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    • pp.7-12
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    • 2002
  • 본 연구는 2001년 5월과 7월에 순천지역의 한 농장을 대상으로 종모돈 64두와 미국의 SGI회사로부터 직수입된 동결정액을 가지고 인공수정시 정액의 형태와 방법이 종모돈의 번식성적에 미치는 영향을 구명하고자 실시한 바, 그 얻어진 결과는 다음과 같았다. 1. 일반적인 액상정액을 이용한 인공수정과 자궁내 이식기구를 이용한 동결정액의 이용이 번식성적에 미치는 영향을 조사한 결과는, 분만율에서는 액상정액을 이용한 처리구 (86.4%)가 동결정액을 이용한 처리구 (67%)보다 높게 나타났다. 산자수와 이유두수는 동결정액을 이용한 처리구 (9.7 및 9두)가 액상정액을 이용한 처리구 (9.29 및 8.8두)보다 높은 수치를 보였으나 유의적인 차이는 없었다. 2. 동결정액을 모돈의 산차별로 구분하고 인공수정하여 얻은 분만율에서는 3∼5산차에서 6두를 공시하여 6두 모두 임신에 성공하여 100%의 분만율을 보였으나, 6∼10산차에서는 4두를 공시하여 1두만이 분만되었다. 그리고 산자 수와 이유두수에서 0∼2산차 (11.3 및 9.3두), 6 ∼ 10산차 (8 및 8두)로 산차가 높을수록 전체적인 번식성적이 낮아지는 수치를 보였으나 유의적인 차이는 없었다. 이상의 결과에서 동결정액을 이용한 인공수정시 자궁심부까지 주입하는 자궁내 인공수정 기술을 이용함으로 임신율과 산자수수 및 이유 두수에서 신선정액을 자궁경관에 주입하는 인공수정방법과 유의차를 보이지 않았을 뿐만 아니라 대등한 결과를 얻을 수 있었다. 동결정액은 자궁내 인공수정기술 방법과 함께 이용 할 수 있는 그 가능성을 제시할 수 있다고 판단되었다.

칡소 동결 정액 생산을 위한 스티로폼상자와 액체질소 이용 방법 (The Use of Styrofoam Box for Chikso (Korean Brindled Cattle) Semen Cryopreservation with Liquid Nitrogen)

  • 김성우;고응규;이재영;김찬란;황인설
    • 한국산학기술학회논문지
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    • v.21 no.4
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    • pp.490-496
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    • 2020
  • 가축유전자원으로서 동결정액을 생산하는 가장 쉬운 방법은 스티로폼박스를 이용한 간이동결법으로 알려져 있다. 본 연구에서는 스티로폼 동결박스를 제작하여 가축의 동결정액 생산에 활용하는 방법을 검토하였으며 칡소 동결정액 생산을 최적화 할 수 있는 방법을 제시하고자 박스의 크기, 액체질소와 노출된 정액 스트로와 거리, 노출시간 및 생산량을 검토하였다. 2가지 동결박스를 비교하여 자료를 확보하였으며 내부 크기는 세로×가로×높이가 23.5×30.5×22.5 cm와 25.5×46.5×26.5 cm로 측정되었다. 액체질소를 5cm 높이로 채우고 액체질소 위 2, 5 및 8cm 높이에서 동결하여 융해 후 생존성을 조사하였다. 칡소 정액을 동결할 경우, 액체질소와의 노출시간은 모두 10분이 적절하였으며 25.5×46.5×26.5 cm 크기의 상자가 높은 생존율을 보여주었다(60.4±5.3% 대 67.2±3.1%). 동결 상자의 최적화 공간은 정자 동결에 가장 중요한 요소로 판단되며 1회 동결 시 최대 생산 가능한 칡소 동결정액은 60개 이상으로 증가시킬 수 있었다. 이러한 정보를 활용하면, 축종에 따라 동결 정액 생산량 결정하고 목적에 맞는 용기를 활용하여 효율적인 동결정액 생산이 가능할 것으로 판단된다.

개 정액의 정제화동결법과 Straw 동결법에 관한 비교실험 (A Comparison between Pellet and Straw Methods in Canine Semen Freezing)

  • 이정원;김희은;김남수;최인혁
    • 한국임상수의학회지
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    • v.8 no.2
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    • pp.183-190
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    • 1991
  • Pellet and straw methods in canine semen freezing are compared with respect to motility, viability and acrosome demage of sperm during each of the two major processing steps, to prior-freezing and to frozen-thawing. Senen was extended with a tris-buffered egg yolk contained 4% glycero1 Pellet freezing in the hole of dry ice and straw freezing on the surface of liquid nitrogen were carried out, respectively. The frozen semen 10 days after storage in liquid nitrogen container. wao thawed. In the comparison of two freezing methods, the straw freezing method with 42.7% in motility. 49.2% in viability and 0.186 acrosome score after thawing seems to be superior to the pellet freezing method with 31.2%, 34.5% and 0.314%, respectively. Sperm motility of processing step to frozen-thawing against decrease rate 12.67% to Prior freezing appeared of 33.84% and 49.37% in straw and pellet freezing and increase of 0.02 in acrsomal score to prior freezing appeared of 0.08 and 0.21 in straw and pellet freezing method to frozen-thawing

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Developement of Quantitative Extraction Method of Amygdalin without Enzymatic Hydrolysis from Kyonin(Armeniacae Semen) by High Performance liquid Chromatography

  • Kim, Dong-Min;Hong, Seon-Pyo
    • 대한약학회:학술대회논문집
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    • pp.388.3-389
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    • 2002
  • Kyonin(Armeniacae Semen)is the herb medicine that contains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde. HCN. and glucose by emulsin. a hydrolysis enzyme in kyonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula kyonin was 0.5% from crude powers. 0.7% from small pieces. 1.2% from half pieces and 2.7% from whole pieces. (omitted)

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Effects of Glycerol Concentration on Viability of Frozen-thawed Canine Spermatozoa

  • Shin, Young-Jee;Son, Jung-Min;Lim, Young-Hwan;Kim, Young-Sil;Lee, Doo-Soo;Yoon, Ki-Young;Shin, Sang-Tae;Cho, Jong-Ki
    • 한국수정란이식학회지
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    • v.23 no.2
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    • pp.115-118
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    • 2008
  • Glycerol is the cryoprotectant most frequently used to freeze semen in several of species. The objective of the present study was to compare the effect of three different glycerol concentrations (4, 6 or 8%, v/v) on frozen-thawed dog sperm survival rate. Ejaculates from 9 dogs collected by digital manipulation were pooled and assessed by macroscopic and microscopic criteria. Semen was divided into 3 aliquots, which were centrifuged and the sperm pellets rediluted with first Tris-glucose-citric acid extender. After 1 h cooling at $4^{\circ}C$, second extender containing 4, 6 or 8% glycerol was added, respectively. The semen was loaded into 0.25 ml straws and frozen and stored in liquid nitrogen and thawed. Sperm vigor, live:dead spermatozoa ratio using HOS test, and sperm morphology using $Spermac^{(R)}$ stain were evaluated. After thawing, there were no significant differences among groups in vigor, viability and morphology. In conclusion, the three glycerol concentrations (4, 6 or 8%) can be used successfully in cryopreservation of canine semen. Therefore the use of 4% glycerol in the extender has less toxic effect and reduces of freezing injuries.

가금의 인공수정 (Artificial Insemination in Poultry)

  • Howarth, Birkett
    • 한국가축번식학회지
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    • v.7 no.2
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    • pp.57-71
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    • 1983
  • 1. Diluted chicken semen can be preserved at 2 to 5$^{\circ}C$ for 24 to 48 hr with resultant fertility of greater than 90% of that of fresh semen. Turkey semen can be preserved at 10 to 15$^{\circ}C$ for 6 to 24 hr and provide economical fertility. 2. Frozen chicken semen has given variable results; a 21 to 93% fertility ranges as compared to 92 to 94% expected with fresh semen. Highest fertility levels obtained with frozen turkey semen intravaginally inseminated have been 61 and 63% using DMSO and glycerol, respectively, as cryoprotectants. 3. The use of glycerol as a cryoprotectant reauires that its concentration in semen be reduced to less than 2% either by dialysis or centrifugation after thawing and before intravaginal insemination if optimal fertility is to be obtained. 4. The temperature at which cryoprotectants are added to semen and the time allowed for equilibration are important for subsequent fertility pre- and post-freezing. 5. The type of container used for packaging the semen, freeze or cooling rates, thaw rates and level of cryoprotectant all interact in affecting cell survival. 6. Plastic freeze straws as a packaging device for semen offers the following advantages: easy to handle, require minimal storage space, offer a wide range of freeze and thaw rates, and insemination can be made directly from them upon thawing. 7. Controlled slow cooling rates of 1 to 8$^{\circ}C$/min have thus far provided the best results for cooling chicken semen throught the transition phase change (liquid to solid) or critical temperature range of +5 to -20 or -35$^{\circ}C$. 8. Highest fertilities have been achieved with frozen chicken semen where a slow thaw rate (2。 to 5$^{\circ}C$) has been used regardless of the freeze rate. 9. To maintain a constant high level of fertility throughout a breeding season with frozen semen, a higher absolute number of spermatozoa must be inseminated (2 to 3 times as many) as compared to fresh semen since a, pp.oximately 50% are destroyed during processing and freezing. 10. The quality of semen may vary with season and age of the male. Such changes in sperm quality could be accentuated by storage effects. Thus, the correct number of spermatozoa may very well vary during the course of a breeding period. 11. As to time of insemination, it is best to avoid inseminating chicken hens within 1-2 hr after or 3-5 hr before oviposition; and turkey hens during or 7-10 hr before oviposition. 12. The physiological receptiveness of the oviduct at the time of insemination is a very important biological factor influencing fertility levels throughout the breeding season.

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AndroMed를 이용한 흑우 동결 정액으로 체외수정란 생산 효과 (Effect of Production In Vitro Embryo with Frozen-thawed Semen using AndroMed Extender in Korean Black Cow Semen)

  • 조상래;최선호;최창용;손준규;김재범;김성재;손동수;김현종
    • 한국수정란이식학회지
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    • v.24 no.3
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    • pp.207-212
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    • 2009
  • The aim of present experiment was to examine commercial synthetic extender(AndroMed) for semen cryopreservation of Korean Black Bull. Semen was collected from a Korean Black Bull using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by AndroMed. The pellect was diluted to final sperm concentration of $5{\times}10^5/ml$ by doubling in every 10 minutes at $4^{\circ}C$ cold chamber. The semen was equilibrated for 1 hr at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 minutes and above 10 cm for 10 min. And then the frozen straw was plunged to $LN_2$. The presented straws were examined the viability and motility after thawed at $37^{\circ}C$ water bath. Hanwoo semen was used as KPN (Korea Proven Bull Number) in this experiment. The survival rates was significantly higher in fresh semen than frozen semen ($80{\pm}14%\;and\;43{\pm}11%$). However, the motility rates was similar (80.7% and 66.4%). The survival and motility rates were higher in 5cm, 10 min treatment group than the other two groups in straw-located height and duration above $LN_2$ ($50{\pm}14%$ and 70.7% vs, 33.18% and $65{\pm}7%$ vs, 30.14% and 65.7%, respectively). The development rates to cleavage was higher in Black Cow than Hanwoo semen (62.2%, 64.4%), However, The development rates to blastocyst was higher in Hanwoo than Black cow semen (25.9%, 23.0%). In conclusion. The present results that acceptable fertilization and cryopreservation could be obtained by in vitro fertilization with frozen-thawed semen using a synthetic semen extender (AndroMed).