• Title/Summary/Keyword: Liplase-100T polyunsaturated fatty acids(PUFAs)

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Investigation of the Hydrolysis Characteristics of Fish Oil by Means of Aspergillus oryzae Lipase Lipolase-100T (Aspergillus oryzae 유래의 리파제 Lipolase-100T에 의한 물고기 기름의 가수분해 특성 규명)

  • 우동진;조귀준;허병기
    • KSBB Journal
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    • v.14 no.3
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    • pp.259-263
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    • 1999

  • Fish oil was hydrolyzed with Aspergillus oryzae lipase, Lipolase-100T. The hydrolysis characteristics of Lipolsae-100T were investigated. Lipolase-100T showed 1,3-positional specificity which hydrolyzed acyl chains combined on the 1 or 3 position of triglyceride into free fatty acids. Lipolase-100T represented another property that the saturated fatty acids composing the triglyceride were hydrolyzed more easily that the polyunsaturated fatty acids(PUFAs). n-3 PUFAs, such as C16:4, C20:5 and C22:6, were hardly hydrolyzed, so that the concentrations of those in the mixture of glycerides were increased according to hydrolysis time. Especially docosahexaenoic acid(DHA), C22:6 showed the highest increase in the concentration. This result explained that n-3 PUFAs were combined on 2-position of triglyceride. When the hydrolysis of fish oil with Lipolase-100T 0.4 wt% was performed for 120 hr, n-3 PUFAs wt% was increased to 50 wt% in the mixture of glycerides. This result was obtained due to the 1,3-positional specificity of Lipolase-100T and positional specificity of n-3 PUFAs.

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