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Detailed Mode of Action of Arabinan-Debranching α-ʟ-Arabinofuranosidase GH51 from Bacillus velezensis

  • Oh, Gyo Won;Kang, Yewon;Choi, Chang-Yun;Kang, So-Yeong;Kang, Jung-Hyun;Lee, Min-Jae;Han, Nam Soo;Kim, Tae-Jip
    • Journal of Microbiology and Biotechnology
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    • 제29권1호
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    • pp.37-43
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    • 2019
  • The gene encoding an ${\alpha}-{\text\tiny{L}}-arabinofuranosidase$ (BvAF) GH51 from Bacillus velezensis FZB42 was cloned and expressed in Escherichia coli. The corresponding open reading frame consists of 1,491 nucleotides which encode 496 amino acids with the molecular mass of 56.9 kDa. BvAF showed the highest activity against sugar beet (branched) arabinan in 50 mM sodium acetate buffer (pH 6.0) at $45^{\circ}C$. However, it could hardly hydrolyze debranched arabinan and arabinoxylans. The time-course hydrolyses of branched arabinan and arabinooligosaccharides (AOS) revealed that BvAF is a unique exo-hydrolase producing exclusively ${\text\tiny{L}}-arabinose$. BvAF could cleave ${\alpha}-(1,2)-$ and/or ${\alpha}-(1,3)-{\text\tiny{L}}-arabinofuranosidic$ linkages of the branched substrates to produce the debranched forms of arabinan and AOS. Although the excessive amount of BvAF could liberate ${\text\tiny{L}}-arabinose$ from linear AOS, it was extremely lower than that on branched AOS. In conclusion, BvAF is the arabinan-specific exo-acting ${\alpha}-{\text\tiny{L}}-arabinofuranosidase$ possessing high debranching activity towards ${\alpha}-(1,2)-$ and/or ${\alpha}-(1,3)-linked$ branches of arabinan, which can facilitate the successive degradation of arabinan by $endo-{\alpha}-(1,5)-{\text\tiny{L}}-arabinanase$.

Description-Based Multimedia Clipart Retrieval in WWW

  • Kim, Hion-Gun;Sin, Bong-Kee;Song, Ju-Won
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송공학회 1998년도 Proceedings of International Workshop on Advanced Image Technology
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    • pp.111-115
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    • 1998
  • The Internet today is teemed with not only text data but also other media such as sound, still and moving images in a variety of formats. Unlike text, however, that can be retrieved easily with the help of numerous search engines, there has been few way to access data of other media unless the exact location or the URL is known. Multimedia data in the WWW are contained in or linked via anchors in the hyper-documents. They can most reliably be retrieved by analyzing the binary data content, which is far from being practical yet by the current state of the art. Instead we present another technique of searching based on textual descriptions which are found at or around the multimedia objects. The textual description used in this research includes file name (URL), anchor text and its context, alternative descriptions found in ALT HTML tage. These are actually the clues assumedly relevant to the contents. Although not without a possibility of missing or misinterpreting images and sounds, the description-based search is highly practical in terms of computation. The prototype search engine will soon be deployed to the public service through the prestige search engine, InfoDetective, in Korea.

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텍스트 네트워크 분석을 활용한 고령친화 분야의 연구동향 분석 : 「보건의료산업학회지」 게재논문(2007~2018)을 중심으로 (A Study on Research Trends of Age-Friendly Using Text Network Analysis : Focusing on 「The Korean Journal of Health Service Management」 (2007-2018))

  • 고민석
    • 보건의료산업학회지
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    • 제13권4호
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    • pp.19-31
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    • 2019
  • Objectives: The purpose of this study was to analyze research trends in age-friendly research and suggest directions for future research. Methods: For this study, 112 articles related to age-friendly research were selected, from 605 published articles in The Korean Journal of Health Service Management (2007-2018). Content analysis and text network analysis were conducted using SPSS 23.0 and NetMiner 4. Results: First, 2 authors (30.4%) and 4 keywords (45.5%) were the most studied. Most of the studies used quantitative research (93.8%). Primary data (61.9%) and SPSS (77.7%) were the most used for analysis. Second, there were seven common keywords in the top 10 in all the centralities. They were Elderly, Geriatric Hospital, Depression, Care Workers, Long-Term Care Facilities, Experience, and Attitude. Conclusions: This study shows the need for diversity of research topics, subjects, research methods, and analytical tools in future age-friendly related studies. In addition, it suggests activating convergence research in this field linked to various industries and services.

재난약자의 스마트폰 재난정보 활용 욕구에 관한 연구 : 영등포전통시장 사례 중심으로 (A Study on Disaster Vulnerable People who Desire to Use Smartphone for Disaster Information : Focusing on Yeongdeungpo Traditional Market Case)

  • 박상길;최재붕
    • 한국IT서비스학회지
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    • 제18권3호
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    • pp.1-15
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    • 2019
  • Disadvantaged from disaster response training and disaster safety education who people are vulnerable to disasters when faced with disasters. The vulnerability of these disaster response will be linked to the desire for disaster information. Therefore, using smartphone to provide timely disaster information will be an effective disaster response service that enables disaster vulnerable people to avoid disaster efficiently. In particular, the importance of using smartphone as a part of the body will become more and more important in everyday situations when information is acquired through smartphone. In this study, Through a survey of traditional market traders, I can find out that the desire of elderly women, who are weak in disaster, to receive disaster information through smartphone text service is relatively strong. Based on the results of this study, I expect that the opportunity to improve disaster response capability through smartphone text service will be provided to elderly women who are weak in disaster.

OryzaGP: rice gene and protein dataset for named-entity recognition

  • Larmande, Pierre;Do, Huy;Wang, Yue
    • Genomics & Informatics
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    • 제17권2호
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    • pp.17.1-17.3
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    • 2019
  • Text mining has become an important research method in biology, with its original purpose to extract biological entities, such as genes, proteins and phenotypic traits, to extend knowledge from scientific papers. However, few thorough studies on text mining and application development, for plant molecular biology data, have been performed, especially for rice, resulting in a lack of datasets available to solve named-entity recognition tasks for this species. Since there are rare benchmarks available for rice, we faced various difficulties in exploiting advanced machine learning methods for accurate analysis of the rice literature. To evaluate several approaches to automatically extract information from gene/protein entities, we built a new dataset for rice as a benchmark. This dataset is composed of a set of titles and abstracts, extracted from scientific papers focusing on the rice species, and is downloaded from PubMed. During the 5th Biomedical Linked Annotation Hackathon, a portion of the dataset was uploaded to PubAnnotation for sharing. Our ultimate goal is to offer a shared task of rice gene/protein name recognition through the BioNLP Open Shared Tasks framework using the dataset, to facilitate an open comparison and evaluation of different approaches to the task.

COVID-19 recommender system based on an annotated multilingual corpus

  • Barros, Marcia;Ruas, Pedro;Sousa, Diana;Bangash, Ali Haider;Couto, Francisco M.
    • Genomics & Informatics
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    • 제19권3호
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    • pp.24.1-24.7
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    • 2021
  • Tracking the most recent advances in Coronavirus disease 2019 (COVID-19)-related research is essential, given the disease's novelty and its impact on society. However, with the publication pace speeding up, researchers and clinicians require automatic approaches to keep up with the incoming information regarding this disease. A solution to this problem requires the development of text mining pipelines; the efficiency of which strongly depends on the availability of curated corpora. However, there is a lack of COVID-19-related corpora, even more, if considering other languages besides English. This project's main contribution was the annotation of a multilingual parallel corpus and the generation of a recommendation dataset (EN-PT and EN-ES) regarding relevant entities, their relations, and recommendation, providing this resource to the community to improve the text mining research on COVID-19-related literature. This work was developed during the 7th Biomedical Linked Annotation Hackathon (BLAH7).

"단편소설집의 사이클"로서 단 리의 『옐로우』 연구 (Reading Don Lee's Yellow as a Short Story Cycle)

  • 이수미
    • 영어영문학
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    • 제57권5호
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    • pp.727-755
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    • 2011
  • In this paper, I'll try to read Don Lee's Yellow intertextually with a more canonical text, Sherwood Anderson's Winesburg, Ohio, in order to see what kind of traditions and techniques Yellow references and/or rewrites as a way of tracking this production. Yellow's formal properties as a short story cycle are established through its use of particular conventions. For instance, Yellow follows the short story cycle model that includes the assemblage of recurring characters into one locale. Yellow's characters are all connected to and at some point located in the fictional small town of Rosarita Bay, California. The text form aligns it with established literary conventions and traditions and suggests the author's reliance upon or trust in those modes. Yellow's setting in a small town alludes to and has often been compared to Anderson's Winesburg, Ohio, which is perhaps one of the most well-known and extensively discussed short story cycles in American literature. Also following convention is Lee's construction of Rosarita Bay and the text's third person narrator as a member of that town. Both Rosarita Bay and the narrator become important figures through the related-tale nature of the text. The method of story-telling is similar to how the town Winesburg and its "seemingly sympathetic and non-overtly judgmental" narrator are operational in Anderson's text. In sum, Yellow is opportune for intertextual reading largely because it is a collection of stories that create a linked series.

텍스트 마이닝과 소셜 네트워크 분석 기법을 활용한 소비자의 의복 맞음새(Fit)평가에 영향을 미치는 특성 (Using Text Mining and Social Network Analysis to Identify Determinant Characteristics Affecting Consumers' Evaluation of Clothing Fit)

  • 황수현;박주연
    • 감성과학
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    • 제26권1호
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    • pp.101-114
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    • 2023
  • 본 연구의 목적은 텍스트 마이닝과 소셜 네트워크 분석을 활용한 소비자 맞음새 평가의 주요 특징을 규명하는 것이다. 이를 위해 SNS에서 수집된 소비자의 2,000여건의 의복 맞음새 평가 후기로부터 의복 맞음새 관련된 텍스트 데이터를 추출하고 의미연결망 분석과 CONCOR 분석을 수행하였다. 연구 결과, '팬츠'와 '스커트'가 많은 맞음새평가어를 공유하며 다양한 형태로 평가되는 것을 확인하였고 의복의 길이가 가장 많이 평가되었다. 인체부위 중 '허리'는 다양한 의복의 맞음새를 평가하는 가장 중요한 부분이며 의복 맞음새평가어 중 '넓은', '큰', '와이드한', '긴' 등이 가장 많이 사용되는 것으로 나타났다. 본 연구는 소비자 맞음새 평가에 사용된 언어의 구조적 관계와 의미를 구체적으로 규명하고 의복 맞음새의 향상을 위한 실증적 기초 자료를 제공하는데 의의가 있다.

공통의 1차 구조를 가진 우유 지방구막 구성단백질의 당쇄 구조에 관한 생화학적 연구 (Biochemical Studies on the Sugar Chain Structure of Glycoproteins with the Same Protein Core of Bovine Milk Fat Globule Membrane)

  • 석진석
    • Journal of Dairy Science and Biotechnology
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    • 제21권2호
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    • pp.138-147
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    • 2003
  • We here analyzed and proposed the structures of the N-linked sugar chains of PAS-7 from bovine milk fat globule membrane. The N-linked sugar chains were liberated from PAS-7 by hydrazinolysis and, after modifying the reducing ends with 2-aminopyridine (PA), were separated into one neutral (7N,55%) and two acidic (7M mono-, 43%; 7D, di-, 2%) sugar chain roups. The latter were converted into neutral groups (7MN and 7DN) by sialidase digestion. The structure of each of these PA-neutral sugar chains was determined by sugar analysis, sequential exoglycosidase digestion, partial acetolysis, and 1H-NMR spectroscopy. The results show that the 10 sugar chains were of the biantennary complex type with and without fucose. The structure of 7N2A one of the major sugar chains, was proposed as; [structure: see text] A structural comparison between PAS-6 and -7 indicated that although they shared the same protein core, their sugar moiety was markedly different, involving the existence of a different pathway during the post-transcriptional modification.

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