• Title/Summary/Keyword: Linked Treatment

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Vorinostat-induced acetylation of RUNX3 reshapes transcriptional profile through long-range enhancer-promoter interactions in natural killer cells

  • Eun-Chong Lee;Kyungwoo Kim;Woong-Jae Jung;Hyoung-Pyo Kim
    • BMB Reports
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    • v.56 no.7
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    • pp.398-403
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    • 2023
  • Natural killer (NK) cells are an essential part of the innate immune system that helps control infections and tumors. Recent studies have shown that Vorinostat, a histone deacetylase (HDAC) inhibitor, can cause significant changes in gene expression and signaling pathways in NK cells. Since gene expression in eukaryotic cells is closely linked to the complex three-dimensional (3D) chromatin architecture, an integrative analysis of the transcriptome, histone profiling, chromatin accessibility, and 3D genome organization is needed to gain a more comprehensive understanding of how Vorinostat impacts transcription regulation of NK cells from a chromatin-based perspective. The results demonstrate that Vorinostat treatment reprograms the enhancer landscapes of the human NK-92 NK cell line while overall 3D genome organization remains largely stable. Moreover, we identified that the Vorinostat-induced RUNX3 acetylation is linked to the increased enhancer activity, leading to elevated expression of immune response-related genes via long-range enhancer-promoter chromatin interactions. In summary, these findings have important implications in the development of new therapies for cancer and immune-related diseases by shedding light on the mechanisms underlying Vorinostat's impact on transcriptional regulation in NK cells within the context of 3D enhancer network.

Enhancement in the Thermal Stability of Microporous UHMWPE Membrane by LED-UV Crosslinking (LED-UV 가교에 의한 UHMWPE 미세다공막의 열 안정성 향상)

  • Jeong-Hwan Kim;Jinho Jang
    • Textile Coloration and Finishing
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    • v.35 no.4
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    • pp.231-238
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    • 2023
  • Microporous gel films of 29 m were prepared by the gel drawing of UHMWPE/soybean oil blend (4:6, w/w) up to 800%. The stretched films containing the optimal photoinitiator concentration, depending on the film thickness, was cross-linked under 365 nm LED-UV irradiation and the oil was extracted with n-hexane, resulting in a gel fraction of 95 % or more. With the formation of the cross-linked structure, the melt-down temperature and melt-down elongation increased by 16 ℃ and by 63% respectively. Also the thermal stability of the crosslinked UHMWPE was proved by the area shrinkage under the heat treatment decreased to 3.8 % compared to 17.4 % for the pristine film, and by the reductions in the combustion heat. The enhanced thermal stability of the crosslinked UHMWPE microporous membranes can be used for various industrial applications such as filters, electric vehicles and energy storage systems.

Effects of Folic Acid on the Level of Nicotine-Induced Plasma Homocysteine in Rats;In a view of applying the results to Oriental Medicine-Formula for controlling withdrawal symptoms after stopping smoking (엽산 투여에 의한 니코틴-유도 혈장 homocysteine 농도에 대한 영향과 금단증상 완화를 위한 한의학적 처방에 대한 고찰)

  • Park, Yeong-Chul;Shin, Heon-Tae;Park, Hae-Mo;Lee, Sun-Dong
    • Journal of Society of Preventive Korean Medicine
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    • v.11 no.1
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    • pp.45-53
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    • 2007
  • Homocysteine is a sulfur amino-acid produced during the metabolism of the essential amino acid methionine. Moderately increased plasma total homocysteine concentration have been implicated as a risk factor for occlusive vascular disease. Smoking is known to be one of the most significant factors leading to elevated plasma homocysteine concentration. However, the main component of a cigarette, nicotine has been not studied whether it is linked directly to the increase of homocysteine concentration in blood. The metabolism of homocysteine is closely linked to that of its cofactors, folate. Here, the effects of nicotine and folic acid on amount of plasma homocysteine were studied. The concentration of homocysteine was increased by about 70% in rat plasma after nicotine treatment for one month. This increased concentration of homocysteine was reduced by about 60% at 6 hours later after folate treatment. Thus, nicotine should be directly involved in increasing the concentration of plasma homocysteine. Also it is suggested that these results can be and applied and used for controlling withdrawal symptoms after stopping smoking as one of oriental medicine formulas.

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Changes in the Antigenicity of Chicken Egg White by the Treatments of Protease, Trifluoromethanesulfonic Acid, Heat, and NaOH (효소, Trifluoromethanesulfonic Acid, 열, 및 NaOH 처리에 의한 계란 난백의 항원성 변화)

  • Ryu, Ju-Hyune;Lee, Jong-Mee;Shon, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.32 no.3
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    • pp.720-725
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    • 2000
  • To reduce the antigenicity of egg white (EW), EW was treated with several proteolytic enzymes, trifluoromethanesulfonic acid (TFMS), heat, and NaOH. Competitive indirect enzyme-linked immunosorbent assay (ciELISA), using rabbit anti-EW antibody, was performed to examine the antigenicity of the treated EW's. Enzymatic hydrolysis gave no good effect on the reduction of the antigenicity of EW. Neither did the pretreatment with ${\gamma}-irradiation$ before the hydrolysis reduce the antigenicity. TFMS treatment removed the antigenicity of EW. The antigenicity of EW heated at $120^{\circ}C$ for 30 min or treated with NaOH at 0.3% (w/v) and more, decreased to less than 1/10,000 as compared with that of native EW. The combinatory treatment with NaOH, followed by heat at $70^{\circ}C$ for 15 min had a synergic effect on the reduction of the antigenicity of EW.

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Assessment of Estuary Reservoir Water Quality According to Upstream Pollutant Management Using Watershed-Reservoir Linkage Model (유역-호소 연계모형을 이용한 상류 오염원 관리에 따른 담수호 수질영향평가)

  • Kim, Seokhyeon;Hwang, Soonho;Kim, Sinae;Lee, Hyunji;Jun, Sang Min;Kang, Moon Seong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.64 no.6
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    • pp.1-12
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    • 2022
  • Estuary reservoirs were artificial reservoir with seawalls built at the exit points of rivers. Although many water resources can be saved, it is difficult to manage due to the large influx of pollutants. To manage this, it is necessary to analyze watersheds and reservoirs through accurate modeling. Therefore, in this study, we linked the Hydrological Simulation Program-FORTRAN (HSPF), Environmental Fluid Dynamics Code (EFDC), and Water quality Analysis Simulation Program (WASP) models to simulate the hydrology and water quality of the watershed and the water level and quality of estuary lakes. As a result of applying the linked model in stream, R2 0.7 or more was satisfied for the watershed runoff except for one point. In addition, the water quality satisfies all within 15% of PBIAS. In reservoir, R2 0.72 was satisfied for water level and the water quality was within 15% of T-N and T-P. Through the modeling system, We applied upstream pollutant management scenarios to analyze changes in water quality in estuary reservoirs. Three pollution source management were applied as scenarios, the improvement of effluent water quality from the sewage treatment plant and the livestock waste treatment plant was effective in improving the quality of the reservoir water, while the artificial wetland had little effect. Water quality improvement was confirmed as a measure against upstream pollutants, but it was insufficient to achieve agricultural water quality, so additional reservoir management is required.

Effects of mixture extract on allergic contact dermatitis and anti-inflammatory (한방 복합추출물이 알레르기성 접촉피부염과 항염증에 미치는 영향)

  • Jang, Young-Ah;Lee, Jin-Tae
    • The Korea Journal of Herbology
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    • v.29 no.2
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    • pp.55-60
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    • 2014
  • Objectives : The aim of this study was to evaluate whether herb mixture extract (HME) would affect allergic contact dermatitis induced by 1-chloro-2,4-dinitro-chlorobenzene (DNCB) in mice. For this, the level of blood IgE was identified. To evaluate anti-inflammatory effect of HME, we tested HMC-1 cells stimulated by PMA+A231867. Methods : In order to evaluate allergic contact dermatitis effects we observed HME on contact-hypersensitive skin of Balb/cmice induced by 0.5% DNCB and measured concentration of IgE in blood of Balb/c mice. In order to evaluate anti-inflammatory effect of cytokine expression we used the method of enzyme-linked immunosorbent assay. Results : We confirmed deep wounds, erosions, progress of keratinization and drop out of dead skin cells from Balb/c mice induced by 0.5% DNCB. We also observed a remarkable decrease in symptoms of atopic dermatitis in the group that received injection of 200mg/kg HME. In addition, in the measurement outcome for IgE concentration in blood, we confirmed that IgE concentration was increased by treatment with DNCB only, while it was markedly decreased by treatment with HME. We confirmed that cytokine expression decreased through enzyme-linked immunosorbent assay and pERK, pJNK, and pp38 also decreased through western blot test Conclusions : According to the above results, HME has some effect on alleviating symptoms of allergic contact dermatitis and has anti-inflammatory effects.

TGF-β1 protects colon tumor cells from apoptosis through XAF1 suppression

  • JUNG ROCK MOON;SHIN JU OH;CHANG KYUN LEE;SUNG GIL CHI;HYO JONG KIM
    • International Journal of Oncology
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    • v.54 no.6
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    • pp.2117-2126
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    • 2019
  • Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine that functions as a growth suppressor in normal epithelial cells and early stage tumors, but acts as a tumor promoter during malignant progression. However, the molecular basis underlying the conversion of TGF-β1 function remains largely undefined. X-linked inhibitor of apoptosis-associated factor 1 (XAF1) is a pro-apoptotic tumor suppressor that frequently displays epigenetic inactivation in various types of human malignancies, including colorectal cancer. The present study explored whether the anti-apoptotic effect of TGF-β1 is linked to its regulatory effect on XAF1 induction in human colon cancer cells under stressful conditions. The results revealed that TGF-β1 treatment protected tumor cells from various apoptotic stresses, including 5-fluorouracil, etoposide and γ-irradiation. XAF1 expression was activated at the transcriptional level by these apoptotic stresses and TGF-β1 blocked the stress-mediated activation of the XAF1 promoter. The study also demonstrated that mitogen-activated protein kinase kinase inhibition or extracellular signal-activated kinase (Erk)1/2 depletion induced XAF1 induction, while the activation of K-Ras (G12C) led to its reduction. In addition, TGF-β1 blocked the stress-mediated XAF1 promoter activation and induction of apoptosis. This effect was abrogated if Erk1/2 was depleted, indicating that TGF-β1 represses XAF1 transcription through Erk activation, thereby protecting tumor cells from apoptotic stresses. These findings point to a novel molecular mechanism underlying the tumor-promoting function of TGF-β1, which may be utilized in the development of a novel therapeutic strategy for the treatment of colorectal cancer.

Clinical characteristics of COVID-19 (코로나바이러스감염증-19의 임상적 특징)

  • Yoo, Jung Rae;Heo, Sang Taek
    • Journal of Medicine and Life Science
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    • v.17 no.2
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    • pp.33-40
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    • 2020
  • Coronavirus disease 2019 (COVID-19) is a respiratory tract infection caused by a newly emergent coronavirus, that was first recognized in Wuhan, China, in December 2019. Genetic sequencing of the virus suggests that it is a beta coronavirus closely linked to the SARS virus. This disease has non-specific symptoms such as fever, dry cough, sore throat, and gastrointestinal symptoms. This virus can transmit via aerosol and need to droplet precaution to prevent spreading in public areas. Most people with COVID-19 develop only mild or uncomplicated illness. However, about 20% patients require hospitalization, oxygen supply, and intensive care. There is no currently effective treatment available for COVID-19 unresponsive to supportive care. This is review about the recently published epidemiologic, and clinical features, diagnosis, treatment and prevention of COVID-19.

Eosinophil disorders (호산구이상증)

  • Kim, Sun Young
    • Clinical and Experimental Pediatrics
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    • v.52 no.6
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    • pp.643-648
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    • 2009
  • Blood eosinophilia can be classified as either familial or acquired. Familial eosinophilia is a rare autosomal dominant disorder characterized by a stable eosinophil count. Acquired eosinophilia is classified further into a primary or secondary phenomenon depending on whether eosinophils are considered integral to the underlying disease. Primary eosinophilia is considered clonal in the presence of either a cytogenetic abnormality or bone marrow histological evidence of classified hematologic malignancies. Causes of secondary eosinophilia include infections, allergic or immunologic disorders, and drugs. Idiopathic eosinophilia belongs to a category of primary eosinophilia, and this is a diagnosis of exclusion. Cases with eosinophilia that lack evidence of clonality may be diagnosed as idiopathic hypereosinophilic syndrome after all causes of reactive eosinophilia have been eliminated. Genetic mutations involving the platelet-derived growth receptor genes (PDGFRA and PDGFRB) have been pathogenetically linked to clonal eosinophilia, and their presence predicts the treatment response to imatinib. In this review, I will present a clinical summary of both familial and acquired eosinophilia with emphasis on recent developments in molecular pathogenesis and treatment.

Current Insights on Cholangiocarcinoma Research: a Brief Review

  • Mathema, Vivek Bhakta;Na-Bangchang, Kesara
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.4
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    • pp.1307-1313
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    • 2015
  • Colangiocarcinoma (CCA) is a progressively fatal disease which generally occurs due to malignant transformation of hepatic biliary cholangiocytes. The incidence of CCA has been increasing worldwide and there is an urgent requirement for effective diagnosis and treatment strategies against this devastating disease. Different factors including liver-fluke infestation, viral hepatitis, exogenous nitrosamine-mediated DNA damage, and chronic inflammation have been linked to CCA genesis. However, the risk factors and underlying complex mechanisms leading to development of CCA are not sufficiently understood to devise an effective targeted treatment therapy. In this review, we summarize currently known epidemiological and pathological aspects of the disease and briefly describe various potential biomarkers and experimental anticancer phytochemicals related to CCA research. In addition, we also sum up recent findings that link chronic inflammation of hepatic biliary cholangiocytes with CCA. The collective information concisely presented in this article would provide useful insights into the current understanding of this cancer.