• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.385-389
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• 2004

The antibody-mix sandwich enzyme-linked immunosorbent assay (Ab-mix sELlS A) system was developed in order to simultaneously detect the extracellular polysaccharide (FPS) of Aspergillus, Penicillium, or Fusarium species using one detection system. The detection limit and detection range of the Ab-mix sELISA towards EPS of Penicilliun citrinum were not changed, and those towards Fusarium moniliforme EPS were changed a little compared to that of individual sandwich ELISA [9, 10]. The fungal culture filtrates of Aspergillus and Penicillium species showed nearly similar reactivity towards Ab-mix sELISA as that of sELISA using the MAb lB8 alone [9]. Also, the fungal culture filtrates of Fusarium species showed nearly the same reactivity towards Ab-mix sELISA as that of sELISA using the MAb lB8 alone [10]. Thus, this ELISA system showed that the three genera of molds, Aspergillus, Penicillium, or Fusarium, which are three major important molds producing mycotoxins in food or agricultural commodities, could be detected at the same time, using one detection system.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.612-619
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• 2004

A competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed for selective and rapid detection of a glycopeptide antibiotic, teicoplanin (TP). TP was conjugated to bovine serum albumin (BSA) for use as an immunogen. Repeated subcutaneous injections of 0.5 mg of the conjugate was effective in generating specific polyclonal antibody (PAb) toward TP in rabbits, as determined by cdELISA. TP-horseradish peroxidase conjugate (TP-HRP) was used as an enzyme marker. The cdELISA was developed based on a competition reaction between TP-BSA PAb and TP-HRP conjugate. The TP-BSA PAb was highly sensitive (detection limit, 0.3 ng/ml and specific toward teicoplanin, showing no cross-reactivity to other glycopeptide antibiotics including vancomycin. There were good correlations ($r^2$=0.84 and 0.76, respectively) between cdELISA and microbiological assay, and high-performance liquid chromatography. The cdELISA system developed in this work is expected to be useful not only for selective and rapid monitoring of TP but also for study of TP pharmacokinetics.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.1145-1146
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• 2008

Failures of equipments are linked directly to extensive damages of human lives or financial losses from the increasing uses of railway signaling equipments utilizing computers. Then safety organizations have to establish for guaranteeing safety during the system life-cycle. In this paper, we examine the relationship between railway signaling standards and other safety standards for safety meaning establishment.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.11
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• pp.1408-1414
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• 2018

Recently, as a solution to the problem of maintaining system reliability, stability, and quality occurring worldwide, such as activation of smart grid and recognition of super grid and rapid grid interconnection of renewable energy sources HVDC(High Voltage Direct Current) will appear on the front of the electric power system. These concepts are also very important concepts in HVDC systems. When the HVDC system is linked to the existing power system, it is composed of AC/DC/AC conversion device, and these conversion devices are composed of many thyristors. These parts(Devices) are connected in a complicated manner, and they belong to the one with a higher failure rate. However, the problem of establishing the concept of failure rate of HVDC parts directly linked to economic efficiency and the understanding accompanying it are still insufficient. Therefore, in this paper, we establish the meaning of reliability in power system and try to develop a model to analyze and verify the failure rate data of HVDC based on this.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.248-253
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• 1997

The paper describes the activation mode and the glycosic linkage of anti-complementary polysaccharide isolated from Cinnamomum cassia. The polysaccharide fractions, CC-IIIa, CC-IIIb, and CC-IIIc, activated C3 component existed in normal human serum and produced C3 cleavage segments, C3a and C3b. The polysaccharide, CC-2-IIIa-3 activated the complement system both in the presence and absence of $Ca^{++}$, suggesting that it involved in both classical and alternative complement pathways. Methylation of CC-2-IIIa-3 was performed with methylsulphinyl carbanion and methyl iodide in DMSO. The methylated products was hydrolyzed, then converted into the partially methylated alditol acetates. Gas chromatography-mass spectrometry(GC-MS) revealed derivatives of terminal $Glc{\rho}$ and $Gal{\rho}$, 1,2-linked $Rha{\rho}$, 1,6-linked $Man{\rho}$, 1,3-linked $Glc{\rho}$, 1,6-linked $Gal{\rho}$ etc.

• Proceedings of the KIPE Conference
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• 2013.11a
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• pp.119-120
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• 2013

Wind turbines as a power distribution system linked to the driving operation according to the characteristics of the distribution system can be adversely affected by a change of circumstances, depending on Distribution Systems with driving characteristics of a wind turbine can be affected. Thus according to the grid-tied wind turbines and transient suppression of the distribution lines and distribution lines outside of the accident in order to maintain stable power supply to supply reactive power efficient system is necessary to keep the voltage constant. In this paper, we substantiated in Jeju Hangwon 12 Unit Level 1MVA STATCOM system configuration, control, configure, and test results will be introduced.

• Food Science of Animal Resources
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• v.30 no.1
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• pp.36-41
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• 2010

The Hen's egg is widely used in many processed foods as an ingredient and is one of the most prevalent food allergens in children. To detect egg proteins in processed foods, we developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) using an anti-ovomucoid (OM) antibody, which was produced by immunization of rabbits with OM, the most heat-stable component of the egg proteins. The detection limit of this quantitative assay system was 30 ng/mL. Cross-reactivity of the anti-OM antibody toward OM, ovalbumin, skim milk, casein, whey protein isolate, and isolated soy protein was 100, 0.4, 0.2, 0.04, 0, and 0%, respectively. In the spike test of egg white powder in milk replacer, commercial sausage, and in-house sausage, the assay recoveries ($mean{\pm}SD$) were $129{\pm}13.7%$, $73.9{\pm}12.5%$, and $65.5{\pm}13.6%$, respectively. When egg white in a commercial crab meat analog and sausage was determined by ciELISA, the assay recovery was found to be 108% and 127%, respectively. The combined results of this study indicate that this novel ciELISA for OM detection could be applied for the quantification of hen's egg proteins in processed foods.

• KEPCO Journal on Electric Power and Energy
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• v.8 no.1
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• pp.15-19
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• 2022

The radial distribution systems (RDS) commonly used around the world has the following disadvantages. First, when the DL is operated on a radial system, the line utilization rate is usually kept low. Second, if a fault occurs in the radial DL, a power outage of 3 to 5 minutes is occurring depending on the operator's proficiency and fault situation until the fault section is separated and the normal section is replaced. To solve this problem, Various methods have been proposed at domestic and foreign to solve this problem, and in Korea, research is underway on the advanced system of operating multiple linked DL always. A system that is electrically linked always, and that is built to enable high-speed communication during the protection coordination is named networked distribution system (NDS). Because the load shares the DL, the line utilization rate can be improved, and even if the line faults, the normal section does not need to be cut off, so the normal section does not experience a power outage. However, since it is impossible to predict in which direction the fault current will flow when a failure occurs in the NDS, a communication-based protection coordination is used, but there is no backup protection coordination method in case of communication failure. Therefore, in this paper, we propose a protective cooperation method to apply as a backup method when communication fails in NDS. The new method is to change TCC by location of CB using voltage drop in case of fault.

• BMB Reports
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• v.34 no.1
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• pp.80-84
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• 2001

The human papillomavirus E7 protein can form a specific complex with a retinoblastoma tumor suppressor gene product (p105-Rb) that results in the release of the E2F transcription factor, which is critical for the growth-deregulation and transforming properties of the viral E7 oncoprotein. In an attempt to apply interaction between the E7 oncoprotein and a target cellular protein Rb for an in vitro screening system for drugs against human papillomavirus infection, we primarily investigated the E7Rb binding through a pull down assay and enzyme-linked immunosorbent assay. The pull down assay showed that both glutathione S-transferase-tagged E7 and His-tagged E7 immobilized on resins specifically produced complexes with bacterially expressed Rb in a dose-dependent manner, as determined by immunoblot analyses. This result coincided with that of an enzyme-linked immunosorbent assay, which is a useful system for the mass screening of potential drugs. Taken together, this screening system (based on the interaction between E7 and Rb) can be a promising system in the development of drugs against cervical cancers caused by human papillomavirus infection.

• Toxicological Research
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• v.17
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• pp.197-199
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• 2001

Enhancement of antigen-specific IgE is a hallmark of allergic hyperresponsiveness, therefore it is necessary to adopt or develop a highly sensitive and specific assay for determination of allergen-specific IgE levels in vivo. In this presentation, we introduce an ELISA (enzyme linked immunosorbent assay) system developed to measure the levels of chicken egg ovalbumin (OVA)-specific IgE in serum. The ELISA method uses a commercially available purified rat anti-mouse IgE as a capture Ab and biotinylated OVA as a detection reagent. Avidin-peroxidase with its substrate is used for color development resulting in optical density measurement at 405 nm. The ELISA system produces a highly sensitive dose-response relation-ship between optical density levels and the dilution titer of the OVA-IgE standard serum but no cross-reaction with unrelated IgE or IgG. It is believed that the system is an Efficient tool to delineate an adjuvant effect of environmental pollutants on development of asthmatic and atopic responses.