• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.259-264
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• 1988

Two forms of extracellular inulinase, designated as PI and PII were detected in the crude enzyme preparation from n species of Pseudomonas isolated from soil. PI and PII were purified to homogeneity by ammonium sulfate fractionation, DEAE Sephadex A-50 chromatography, Sephadex G-100 and Sephadex G-200 gel filteration. Both isoenzymes catalyzed specifically and endowise the cleavage of the $\beta$-2,1-fructofranoside linkage of inulin, and displayed no action upon sucrose, raffinose and levan. The optimal pH values for the PI and PII enzyme were pH 5.5 and 6.0, respectively and the highest activity of the two enzymes was observed at 55$^{\circ}C$. The Km values of PI and PII were calculated to be 2$\times$10$^{-3}$M and 5$\times$10$^{-3}$M, respectively.

• Journal of Information Technology Services
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• v.13 no.3
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• pp.343-357
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• 2014

As science and technology infiltrates every aspects of modern society in terms of economic and social growth and development, funding for research and development (R&D) is growing rapidly. Republic of Korea is not an exception in this trend and the R&D funding in Korea has been grown about 10% every year, recently. However, as the scope and size of funding grows exponentially, need for monitoring and managing these R&D projects becoming more and more imminent. Though different types of project management systems were developed by a variety of agencies and departments and used in monitoring and managing, these systems were developed as standalone silo type systems. These systems are not connected to each other while the same researchers may involved in different projects across agencies and department. Also, these management systems are not linked to the banking systems in which real transactions of funding occurs, such as cost reimbursement and financial audit of each R&D accounts. Historically, a few fraud and malappropriation cases were found and indicted. However, as the number of these incidents grows along with the growth of R&D funding, a large scale integration/linking of project management systems and banking systems. Realizing the importance of systems integration among agencies as well as with the banking systems, situational requirements analyses were conducted concerning the current state of R&D management system. As a results, a Real-time Case Management System (RCMS) was proposed as a solution to current problems. In this paper, the collected systems requirements were documents with analyses of the situation, the architecture of the integrated systems with more user-friendly technological alternatives. This large scale linkage requires interface standardization as well as modularization of interfaces. Proposed systems architecture is introduced here with technical details of Jex Framework used,, followed by resulting technical and economic performance of the Realtime Cashflow Management System (RCMS).

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.2
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• pp.83-90
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• 2010

The windshield wiper consists of 4 parts: a blade, an arm, a linkage and a motor. The wiper blade makes contact with the windshield and is designed to be operated normally at an angle of 30~50 degrees to the front glass. If the contact pressure between the wiper blade and windshield surface is too high, noise and wear of the rubber will result. On the other hand, if the contact pressure is too low, the performance will do badly, since foreign substances such as dust and stains will not be removed well. The pressure and friction of the wiper blade has a great influence on its effectiveness in cleaning the front window. This is due to the contact of the rubber with the window. This paper presents the dynamic analysis method to estimate the performance of the flat type blade of the wiper system. The blade has a nonlinear characteristic since the rubber is an incompressible hyper-elastic and visco-elastic material. Thus, Structural dynamic analysis using a complex contact model for the blade is performed to find the characteristics of the blade. The flexible multi-body dynamic model is verified by the comparison between test and analysis result. Also, the optimization using the central composite design table is performed.

• Archives of Pharmacal Research
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• v.21 no.5
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• pp.537-542
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• 1998

to more effectively drive immune responses toward antigen-specific T helper type 2 (Th2) cell-mediated responses, we constructed a mammalian expression vetor (oPVA/IL4) carrying a fused gene in which the ovalbumin (OVA) cDNA was covalently linked to murine interleukin-4 (IL-4) cDNA. A biologically active OVA/IL4 DNA, as demonstrated by Wes tern blotting and cytokine bioassay. In tramuscular injection of BALB/c mice with the pOVA/IL4 DNA increased both the production of OVA-specific IL-4 by CD$4^{+}$ T cells and the ratio of anti-OVA lgG1 to anti-OVA lgG2a isotypes, while the injection with the pOVA DNA alone, or with the mixture of the pOVA and pIL4 DNA did no or little increase. furthermore, the OVA-specific, Th2 cell-mediated immune responses were significantly enhanced by multiple injections with the pOVA/IL4 DNA. These studies indicate that the direct linkage of an OVA gene to an IL-4 gene in the expression plasmid confines the effects of IL-4 to the OVA-specific cells, efficiently driving the immune response toward OVA-specific, Th2 cell-mediated responses.

• The Plant Pathology Journal
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• v.31 no.1
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• pp.33-40
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• 2015

Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN $7_{414}$) and a repulsion phase marker (IABTPPN $7_{983}$) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN $7_{983}$, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN $7_{414}$ did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN $7_{983}$ (P<0.0001) and IABTPPN $7_{414}$ (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in $F_2$ population. The repulsion phase marker, IABTPPN7983, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.

• Journal of Korea Water Resources Association
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• v.37 no.6
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• pp.499-507
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• 2004

In this study, the fully coupled SWAT-MODFLOW model is developed by using the type of embedment MODFLOW in SWAT. Since SWAT model has semi distributed features, its groundwater component can't consider distributed parameters such as hydraulic conductivity, storage coefficient and spatially variable natures such as distribution of groundwater heads and pumping rate and so forth. The main purpose of this study is to overcome these limitations. This linkage is completed considering the interaction between stream network and aquifer to reflect boundary flow. To correspond HRU in SWAT to grid in MODFLOW, HRU-GRID conversion tool using DEM is newly suggested. As groundwater recharge of MODFLOW can be estimated accurately by SWAT model, the reliability of groundwater discharge and total runoff of watershed could be greatly enhanced.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.36-42
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• 2002

Sixteen Atractylodes japonica Koidz. accessions were collected in Jeonbuk, Kyeongnam, Kangwon and Kyeonggi province, and their quantitative characteristics were compared with each other. The relationship of variation between Atractylodes japonica Koidz. accessions was also investigated by means of multivariate analysis. Hamyang 1 accession had the most desirable quantitative characteristics among the accessions: plant height was 86.2cm, number of leaves, 117.2 and number of stems, 34.3. Sudo 1 accession had 12 stems, the most among the accessions. The length of leaf margin serras found in all of them was on average 1.6mm. Most accessions had simple or palmately compound leaf with oval or elliptical type of the leaf shape. The accessions could be classified into three groups by average linkage cluster method. The most remote relationship was found between Group 1 containing Jinan 1, Jinan 2 and Group 3 containing Jeongsu, and Geoje.

• Journal of Korea Water Resources Association
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• v.53 no.spc1
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• pp.773-784
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• 2020

In this study, legislative and policy issues related to advancing the maintenance system of water resource facilities were presented by investigating and analyzing the degree of aging and management status of water resource facilities. Data from the comprehensive Facility Management System (FMS) operated by the Korea Infrastructure Safety Corporation were collected and used to analyze the aging of water resource facilities. Using safety grade data categorized by facility, type, local area, and grade, the ratio of dangerous facilities and the aging rate were calculated to analyze the status of water resource facilities and guidelines and manuals related to water resource facilities were investigated. Through this, four required actions to address legislative and policy issues were presented: 1) Readjusting the scope of water resource facilities and supplementing guidelines and manuals, 2) Establishing a maintenance system considering changes in disaster conditions, 3) Improving the FMS and activating standard linkage services, and 4) Establishing a facility asset management system.

• The Journal of the Korea Contents Association
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• v.18 no.6
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• pp.333-341
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• 2018

Recently, there has been a growing interest in user-centered smart city services. This study examines the spatial information type to derive a smart city service scenario through space - based communication and aims to establish a direction for user - centered experience factor scenario. The results of this study are meaningful for presenting basic data to suggest a space scenario where citizens can experience smart city as walking map and experience route applicable to future smart city test bed. In particular, we simulated the walking scenarios in the smart city test bed through Walking Map, which is tested the service direction based on user requirements. Through this study, we confirmed that smart city service through existing infrastructure can be used multipurpose. The spatial information and the experience factor linkage model and the walking map through the persona model presented in this study can be utilized as a preliminary scenario applicable to the future smart city test bed.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.270-276
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• 2016

Extended-spectrum β-lactamases (ESBLs), particularly those of the CTX-M types, are the predominant resistance determinants of Escherichia coli that are rapidly spreading worldwide. To determine CTX-M types, E. coli isolates were collected from retail chickens (n = 390) and environmental samples from chicken farms (n = 32) and slaughterhouses (n = 67) in Korea. Fifteen strains harboring bla_CTX-M genes were isolated from 358 E. coli isolates. The most common CTX-M type was eight of CTX-M-15, followed by six of CTX-M-1 and one of CTX-M-14. The bla_CTX-M genes were identified in the isolates from retail chickens (n = 9), followed by feces, water pipes, floors, and walls. Conjugations confirmed the transferability of the plasmids carrying bla_CTX-M genes to the recipient E. coli J53 strain. Furthermore, eight addiction systems carried by the replicons in CTX-M types were confirmed. The dominant system was identified as ccdAB, vagCD, and pndAC in donor strains and transconjugants. The clonal relationship between the two strains carrying bla_CTX-M genes indicates that E. coli may transmit from the farm to retail chickens, suggesting a possible public health risk. Our findings demonstrate that the detection of CTX-M types in E. coli isolates is important for tracking ESBL production in animals, and suggest linkage of multiple addiction systems in plasmids bearing bla_CTX-M genes.