• Korean Journal of Medicinal Crop Science
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• v.9 no.4
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• pp.301-309
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• 2001

This study was conducted to provide the basic information on safflower collections and to identify the variations which could be utilized in safflower breeding programs. The agronomic characteristics was used to clarify the genetic relationships among safflower collections and to classify them into distint genetic groups. There were 21 early maturing collections with less than 80 days in number of days from planting to flowering. The number of primary and secondary branches ranged ${3.8{\sim}14.8\;and\;0{\sim}26.9}$, respectively, and two collections, IT201434 and IT202723, were found to be high branch types. The 101 safflower collections were classified into 11 groups based on the complete linkage cluster analysis using agronomic characteristics. The I, II, III, IV, IX, X and ? groups included the 25%, 33%, 14%, 8%, 2%, 1% and 1% of the collections, respectively. All the collections in the group III were Korean landraces. The collections in group X could be characterized as early emergence, late flowering and high yield components such as the number of capitula per plant, number of seeds per capitula and seed weight per plant. The number of capitula per plant and seed weight per plant, i.e., the two main yield components, had the highly significant positive correlations with stem diameter, number of the primary branches, number of the secondary branches, number of leaves and leaf length.

• Journal of Environmental Impact Assessment
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• v.28 no.2
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• pp.101-112
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• 2019

Over the last few years, air pollution ($PM_{10}$, $PM_{2.5}$) in the Seoul metropolitan area (SMA) has emerged as one of the most concerned and threatening environmental issues among the residents. It brings about various harmful effects on human health, as well as ecosystem and industrial activities. Governments and individuals pay various costs to mitigate the level of air pollutants. This study aims to empirically find the willingness to pays (WTP) among the parents from different socio-cultural groups - international and domestic groups to mitigate air pollution ($PM_{10}$, $PM_{2.5}$) in their residential area. Contingent Valuation Methods (CVM) is used with employing single-bounded dichotomous choice technique to elicit the respondent's WTP. Using tobit (censored regression) and probit models, the monthly mean WTP of the pooled sample for green electricity which contributes to improve air quality in the region was estimated as 3,993 KRW (3.58 USD). However, the mean WTP between the international group and domestic group through a sub-sample analysis shows broad distinction as 3,325KRW (2.98 USD) and 4,449 KRW (3.98 USD) respectively. This is because that socio-cultural characteristics of each group such as socio-economic status, personal experience, trust in institutions and worldview are differently associated with the WTP. Based on the results, the society needs to raise awareness of lay people to find a strong linkage between the current PM issue and green electricity. Also, it needs to improve trust in the government's pollution abatement policy to mobilize more assertive participation of the people from different socio-cultural background.

• Journal of agriculture & life science
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• v.45 no.3
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• pp.25-33
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• 2011

This study was conducted to examine genetic variation on leaf characteristics of Machilus thunbergii populations. Ten populations were subjected to multivariate analysis for 9 characteristics of leaf morphology. Average length of leaf blade, leaf width, petiole length, vein number were 9.8cm, 4.0cm, 1.8cm, 8.4 respectively, while angle of leaf base and leaf apex were $67.9^{\circ}$ and $78^{\circ}$ respectively. The coefficient of variation (C.V.) on leaf characteristics was 20% which indicate similar features among the populations. Nested analysis showed statistically signigicant differences among populations as well as among individuals within populations. Genetic relationship between populations using complete linkage method showed four groups to Euclidean distance 1.2 and did not show a tendency to cluster into the same group. There were three principal components that had a meaningful eigenvalue over 1.0 among the 9 components. The explanatory power of the top three main components on the total variation was 92.8%. The first principal component (PC) was explained about 40.3% which is mainly correlated with maximum leaf width and the second PC was explained about 28.7% which is correlated with leaf blade length. The third PC was explained about 23.8% which is correlated with petiole length ($X_3$). These characters were important factors for analysis of the relationship among natural populations of M. thunbergii.

• Journal of Korean Society of Forest Science
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• v.90 no.4
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• pp.558-564
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• 2001

For the study of morphological variation in winter buds of Q. variabilis $B_L$. natural populations in Korea, 16 populations were selected through the country in consideration of latitude, longitude, altitude, and geographical characters. Thirty trees were randomly selected from each population and 30 terminal and 30 lateral buds were sampled below 1/3 crown length of each tree. Four morphological characters including length and width of terminal and lateral buds were measured. 1. Length and width of terminal and lateral buds were in the ranges 6.9~11.3cm, 3.0~3.7cm, 6.1~8.9cm, and 2.5~3.1cm, respectively. The coefficient of variation were in about 20% in all the characters investigated. 2. All the characters were significantly different among populations as well as among individuals within populations. The degree of contribution of variance among individuals within populations was higher than that among populations. 3. Length of terminal bud showed positive correlation with length and width of lateral bud; and width of lateral bud with width of terminal bud and length of lateral bud. Also, positive correlations were observed between longitude and width of terminal bud, between latitude and length of terminal and lateral bud. 4. Cluster analysis using complete linkage method for winter bud characters showed two groups to Euclidean distance 3.4. They were group I of population 8 and group II of populations 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, and 16. However, group II was divided into two at Euclidean distance 1.5 that are a group including populations 1, 2, 7, 9, and 11(groupII-1) and the other group including populations 3, 4, 5, 6, 10, 12, 13, 14, 15, and 16(groupII-2).

• The korean journal of orthodontics
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• v.33 no.3 s.98
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• pp.185-197
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• 2003

This study was performed to identify the characteristics of the OFC1 gene (locus: chromosome 6p24.3) in Korean patients, which is assumed to be the major gene behind the nonsyndromic cleft lip and palate. The sample consisted of 80 subjects: 40 nonsyndromic cleft lip and palate patients (proband, 20 males and females, mean age 14.2 years); and 40 normal adults (20 males and 20 females, mean age 25.6 years). Using PCR-based assay, the OFC1 gene was amplified, sequenced, and then searched for similar protein structures. Results were as follows: 1. The OFC1 gene contains the microsatellite marker 'CA' repeats. The number of the reference 'CA' repeats was 21 times, and formed as TA(CA)11TA(CA)10. But, in Koreans, the number of tandem 'CA' repeats was varied from 17 to 26 except 18, and 'CA' repeats consisted of TA(CA)n. 2. Nine allelic variants were found. Distribution of the OFC1 allele was similar between the patients and control group. 3. There was a replacement of the base 'T' to 'C' after 11 tandem 'CA' repeats in Koreans compared with Weissenbach's report. However, the difference did not seem to be the ORF prediction results between Koreans and Weissenbach's report. 4. The BLAST search results showed the Telomerase reverse transcriptase (TERT) and the Nucleotide binding protein 2 (NBP2) as similar proteins. The TERT was a protein product by the hTERT gene in the locus 5p15.33 (NCBI Genome Annotation; NT023089) The NBP2 was a protein product by the ABCC3 (ATP-binding cassette, sub-family C) gene in the locus 17q22 (NCBI Genome Annotation; NT010783). 5. In the Pedant-Pro database analysis, the predictable protein structure of the OFC1 gene had at least one transmembrane region and one non-globular region.

• Korean Journal of Biological Psychiatry
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• v.21 no.3
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• pp.99-106
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• 2014

Objectives Previous studies suggest that the cannabinoid receptor 1 (CNR1) gene could be an important candidate gene for schizophrenia. According to linkage studies, this gene is located on chromosome 6q14-q15, which is known to harbor the schizophrenia susceptibility locus (locus 5, SCZ5, OMIM 803175). The pharmacological agent delta-9-tetrahydrocannabinol (${\Delta}$-9-THC) seems to elicit the symptoms of schizophrenia. The association between CNR1 polymorphisms and schizophrenia is actively being investigated, and some studies have linked the AAT-trinucleotide repeats in CNR1 to the onset of schizophrenia. In this study, we have investigated the association between the AAT-trinucleotide repeats in CNR1 and schizophrenia by studying schizophrenia patients and healthy individuals from Korea. Methods DNA was extracted from the blood samples of 394 control subjects and 337 patients diagnosed with schizophrenia (as per the Diagnostic and Statistical Manual of Mental Disorders, fourth edition criteria). After polymerase chain reaction amplification, a logistic regression analysis, with age and gender as the covariates, was performed to study the variations in the AAT-repeat polymorphisms between the two groups. Results In total, 8 types of trinucleotide repeats were identified, each containing 7, 8, 10, 11, 12, 13, 14, and 15 repeats, respectively. $(AAT)_{13}$ allele was most frequently observed, with a frequency of 33.6% and 31.6% in the patient and control groups, respectively. The frequency of the other repeat alleles in the patient group (in the decreasing order) was as follows : $(AAT)_{13}$ 33.6%, $(AAT)_{14}$ 21.6%, $(AAT)_{12}$ 18.5%, and $(AAT)_{7}$ 11.1%. The frequency of the repeat alleles in the control group (in the decreasing order) was as follows : $(AAT)_{13}$ 31.6%, $(AAT)_{14}$ 24.5%, $(AAT)_{12}$ 17.2%, and $(AAT)_{7}$ 11.6%. However, there were no significant differences in the AAT-repeat polymorphisms of the CNR1 gene between the patient group and the control group. Conclusions Although our study revealed no significant association of the AAT-repeat polymorphism of the CNR1 gene with schizophrenia, it will serve as a good reference for future studies designed to examine the cannabinoid hypothesis of schizophrenia.

• Journal of Life Science
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• v.29 no.12
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• pp.1314-1320
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• 2019

This study aimed to identify single nucleotide polymorphisms in exon 8 region of the calmin (CLMN) gene and to evaluate their associations with meat yield and quality traits in Hanwoo (Korean cattle). We compared gene expression pattern of CLMN between high and low marbling score groups with extremely different intramuscular fat content of the longissimus lumborum muscles in Hanwoo. The CLMN gene was highly expressed in the high marbling score group. Total of nine SNPs were identified in the exon 8 region of CLMN gene, genotyping of the SNPs was carried out using direct-sequencing analysis in Hanwoo population (n=300) to evaluate their association with meat yield and quality traits. As a result, g.23249G>C in exon 8 was significantly associated with marbling score. Animals with the CC and GC genotypes had higher marbling score than those with the GG genotype. We constructed haplotypes of CLMN gene by linkage disequilibrium analysis and analyzed association between haplotypes and meat yield and quality traits. Haplotype of CLMN gene was associated with marbling score. As a result, animals with the CC-CC haplotype (g.23249G>C and g.23465T>C SNPs) had higher marbling score than those with CT and GT haplotypes. These findings suggest that the SNPs of bovine CLMN gene may be a useful molecular marker for selection of meat quality traits in Hanwoo.

• Journal of the Korean association of regional geographers
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• v.15 no.5
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• pp.671-687
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• 2009

This paper aims to link the development strategy of a local government(Guri-si, Kyunggi-do) to a spatial decision support system that is in the early stages of implementation and testing. Spatial Decision for a local governmental authority typically involves multiple stakeholders. To any specific regional problem, stakeholders often have various levels of knowledge and experiences about it, that yield conflicting views. In this point of view, the enhanced access to information can bring the decision process to solve the problem throughout more public participation. Recently, Urban development study was required to improve the decision making of public participation form. This study aim to investigate an analysis local government of Guri-si, can be supported as the suggestion of decision making system. Guri-si has been argued that it would improve citizen's life quality intent theme 'by historical tourism in general dimension' promote region identity, environment friendly and transportational strength, by reflecting various plan of urban development and it executed strategy. There are increasing interest in citizen participation and practical using of policy decision making to respond to uncertain environment and systematic exploration of local government. Focused on the contents of public participation decision making, it is important to investigate how to analysis urban development strategy and geographic information relationship for useful. The paper concludes with a discussion of current issues encountered during development and implementation of spatial decision making(or support) system for the local administrative authority.

• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.107-116
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• 1999

For the study on the variation of Pinus densiflora S. et Z. natural populations. 13 stands were selected in Kangwondo region in 1994. They were located in Hyoja-Dong, Chunchon-Si [plot 1]; Sinnam-Myun, lnje-Gun [plot 2]; Buk-Myun, lnje-Gun [plot 3]; Seo-Myun, Yangyang-Gun [plot 4 and 5]; Sonyang-Myun, Yangyang-Gun [plot 6]; Toseong-Myun, Goseong-Gun [plot 7]; Synrim-Myun, Wonju-Si [plot 8]; Dunnae-Myun, Hoengsong-Gun [plot 9]; Jinbu-Myun, Pyungchang-Gun [plot 10]; Sogumgang, Kangneung-Si [plot 11]; Seo-Myun, Ulchin-Gun [plot 12]; and Mitan- Myun, Pyungchang-Gun [plot 13]. The morphological characteristics of needles and cones were investigated for twenty individual trees from each population. The results are summarized as follows: 1. Among needle characteristics, length, the number of serration. and the number of stoma row were significantly different among populations and between individuals within a population. They were 69.3~91.9mm, 25.1~28.7 per 0.5cm, and 4.1~6.2 rows for abaxial side and 2.9~4.6 rows for adaxial side, respectively. 2. Among cone characters surveyed. length and diameter were significantly different among populations and between individuals within a population. They were 31.1~43.7mm and 20.0~24.1mm, respectively. 3. A highly positive correlation was observed between the number of stoma row of abaxial side and that of adaxial side. and between length and diameter of cone. But the number of serration was negatively correlated with all traits. 4. The distance from seashore was positively correlated with needle length and the number of serration, but negatively correlated with the number of stoma row and cone length. However, the altitude was positively correlated with the number of serration, but negatively correlated with the number of stoma row and cone length. 5. Cluster analysis using average linkage method for needle and cone characteristics showed two groups to Euclidean distance 1.0. They were group I consisting of plots 1, 8, 12, and 13 and group II of plots 2, 3, 4, 5, 6, 7, 9, 10, and 11. However, group II was divided again to Euclidean distance 0.8, that is a group including plots 2, 3, 9, and 11 and the other group comprising plots 4, 5, 6, 7, and 10.

• The Korean Journal of Pharmacology
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• v.16 no.2 s.27
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• pp.15-24
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• 1980

[${\alpha}$]-Amylase catalyses the hydrolysis of starch, glycogen, and related poly- and oligosac-charide by random cleavage of ${\alpha}$-D-(l-4) glucan linkage. In man large amounts of amylase are secreted into the digestive tract by the salivary and exocrine pancreatic gland, minimal amount being produced also in other tissues. It has been known that ${\alpha}$-amylase exists in multiple molecular forms, isoenzyme which can be separated from each other because of difference in their physicochemical properties. By using various methods, several groups of investigator have separated the many isoenzyme in serum, saliva and pancreatic juice. Furthermore, changes of the normal serum isoenzyme pattern is diagnostically useful even when the total serum enzyme activity is noninformative, such as the clinical use of isoenzyme of serum lactate dehydrogenase. Procarboxypeptidase-A which is one of the pancreatic enzymes is also present as isoenzymes. Four forms of procarboxypeptidase-A haye been found in the bovine enzyme and three forms of the porcine enzyme. In human pancreatic juice four forms of procarboxypeptidase-A isoenzyme were found by isoelectric focusing method. Recently, the so-called isoamylase analysis was developed for the diagnostic use of amylase in pancreatic diseases. In alcohotic patients, the serum concentration of pancreatic isoamylase is subnormal and this lowered activity provides strong evidence for pancreatic exocrine insufficiency. The purpose of this study was to elucidate the variations of the isoenzyme of amylase and procarboxypeptidase-A in serum, saliva and pancreatic juice of the experimental animals. The results are as follow. 1) Three main forms of isoenzyme of amylase by isoelectric focusing were found in pancreatic juice of normal rabbit. However, many new bands were appeared in the pancreatic juice of cholic acid administered animal intravenously while the infusion of cholic acid or elastase into pancreatic duct produced the decrease of number of the fractions on the isoelectric focusing. In the case of serum isoenzyme from normal animal, two major and a few minor isoamylases were observed. By injecting alcohol intravenousely the fractions of serum isoamylase were significantly decreased and in contrary to the pattern in the pancreatic juice the infusion of cholic acid or elastase into pancreatic duct exhitited a significant decrease of the isoenzyme of amylase fractions. In saliva from normal animal three main isoamylase were produced of the administration of alcohol. 2) In the case of procarboxypeptidase-A isoenzyme, two major fractions which have isoelectric point at 6.2 and 6.4 and other two minor bands were observed in the pancreatic juice of normal rabbit. By the treatment of the juice with trypsin, only one band was produced on the isoelectric focusing. No procarboxypeptidase was appeared on the electrofocusing by the infusion of cholic acid or phospholipase A into the pancreatic duct of rabbit. However, a single major fraction of procarboxypeptidase-A was appeared at 3 hr after simple ligation of the pancreatic duct. No significant changes were observed in the juice of the alcohol or cholic acid administered group.