• KSBB Journal
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• v.15 no.2
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• pp.214-218
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• 2000

A hepatitis B Surface Antigen(HBsAg)-screening kit using immunochromatographic assay(ICA) method was developed by e employing two kinds of antibodies. One is mouse monoclonal anti-HBs for tracer antibody and the other is goat p이yclonal a anti-HBs for capture antibody. This capture antibody was immobilized on the surface of nitroceliulose(NC) membrane and the t tracer antibody was conjugated with g미d particles. When serum sample was added to the sample well, the $\infty$njugates d deposited in a dry state on the surface of glass fiber filter were reconstituted and then combined with HBsAg in serum. In 5 5 min after adding, the assay result was visible through the window, that is, the complexes composed of HBsAg and the c conjugates appeared as maroon line on the lower part of the NC membrane. The detection limit of the ICA kit was 2 ng/ml w when being tested with the reference HBsAg.

• Journal of the Korean Society of Visualization
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• v.8 no.1
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• pp.46-52
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• 2010

In the present study, we employed the confocal laser scanning microscopy (CLSM) system to visualize the blood flow field with $1{\times}1{\mu}m^2$ spatial resolution. Based on the confocal microscopic image of red blood cells (RBCs), we performed the velocity vector field measurement and evaluated characteristics of cell migration from the cell depleted layer thickness calculation. The rat and mouse's blood were supplied into a micro glass tubes in vitro. The line scanning rate of confocal microscopy was 15 kHz for a $500{\times}500$ pixels image. As a result, the red blood cell itself can be used as a tracer directly without any kind of invasive tracer particle to get the velocity vector field of blood flow by performing particle image velocimetry (PIV) technique.

• Analytical Science and Technology
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• v.8 no.4
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• pp.427-434
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• 1995

Inductively coupled plasma mass spectrometry combined with the isotope dilution method is used for the determination of lithium. The isotope dilution method is based on the addition of a known amount of enriched isotope (spike) to a sample. The analyte concentration is obtained by measuring the altered isotope ratio. The spike solution is calibrated through so called reverse isotope dilution with a primary standard. The spike calibration is an important step to minimize error in the determined concentration. It has been found essential to add spike to a sample and the primary standard so that the two isotope ratios should be as dose as possible. Since lithium is neither corrosive nor toxic, lithium is used as a chemical tracer in the nuclear power plants to measure feedwater flow rate. 99.9% $^7Li$ was injected into a feedwater line of an experimental system and sample were taken downstream to be spiked with 95% $^6Li$ for the isotope dilution measurements. Effects of uncertainties in the spike enrichment and isotope ratio measurement error at various spike-to-sample ratios are presented together with the flow rate measurement results in comparison with a vortex flow meter.

• Journal of The Korean Astronomical Society
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• v.39 no.1
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• pp.19-24
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• 2006

We carried out CO survey toward IR-excess clouds using SRAO 6-m telescope in search of molecular $H_2$. These clouds, which show far-infrared excess over what is expected from HI column density, are considered to be candidates of molecular clouds. In order to find new high Galactic latitude clouds, we made mapping observations for 14 IR-excess clouds selected from Reach et al.(1998) in $^{12}CO$ J = 1 - 0 line, supplementing the similar survey in southern hemisphere (Onishi et al. 2001). $^{12}CO$ emission is detected from three IR-excess clouds among 14 objects. Three newly detected clouds exhibit somewhat clumpy morphology and column densities amount to ${\sim}10^{21}\;cm^{-2}$. One of three clouds, DIR120-28, show discrepancy between IR-excess center and CO emission center. It seems that IR-excess may not be an effective tracer of molecular gas. Instead, optical depth$(\tau)$ excess, i.e., IR-excess corrected for temperature dependence, may be more effective tracer of molecular clouds, since, by combining statistics from both hemispheres, we found that the detection rate is higher for IR-excess clouds with lower dust temperature.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.75-78
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• 2015

A growing body of evidence has been supporting the existence of so-called "dark molecular gas" (DMG), which is invisible in the most common tracer of molecular gas, i.e., CO rotational emission. DMG is believed to be the main gas component of the intermediate extinction region from Av~0.05-2, roughly corresponding to the self-shielding threshold of $H_2$ and $^{13}CO$. To quantify DMG relative to $H{\small{I}}$ and CO, we are pursuing three observational techniques; $H{\small{I}}$ self-absorption, OH absorption, and THz $C^+$ emission. In this paper, we focus on preliminary results from a CO and OH absorption survey of DMG candidates. Our analysis shows that the OH excitation temperature is close to that of the Galactic continuum background and that OH is a good DMG tracer co-existing with molecular hydrogen in regions without CO. Through systematic "absorption mapping" by the Square Kilometer Array (SKA) and ALMA, we will have unprecedented, comprehensive knowledge of the ISM components including DMG in terms of their temperature and density, which will impact our understanding of galaxy evolution and star formation profoundly.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.1
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• pp.133-138
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• 2022

In this paper we present the system we produced to help workers at logistic centers and prevent accidents, in which they could get hurt. As a base we use the main control device, named Arduino uno, which measures the weight by using load cells. When operating with the system, by placing object on it, the system measures the weight of the object and keeps the highest point at the same height by lowering its board. This improves the convenience while placing and removing objects from the board. If the weight of the placed object is exceeding the set value the board will also lower itself to secure the safety. By using a line tracer system, the objects are being moved only on a set route with the goal to make it even more comfortable to use.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.410-416
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• 2001

An algorithm of 3-D particle image velocimetry(3D-PIV) was developed for the measurement of 3-D velocity field of complex flows. The measurement system consists of two or three CCD camera and one RGB image grabber. In this study, stereo photogrammetty was applied for the 3-D matching of tracer particles. Epipolar line was used to decect the stereo pair. 3-D CFD data was used to estimate algorithm. 3-D position data of the first frame and the second frame was used to find velocity vector. Continuity equation was applied to extract error vector. The algorithm result involved error vecotor of about 0.13 %. In Pentium III 450MHz processor, the calculation time of cross-correlation for 1500 particles needed about 1 minute.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.97.1-97.1
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• 2012

NIR Emission lines from singly-ionized Iron, in particular [Fe II] $1.64{\mu}m$, are good tracer of dense atomic gas in star-forming regions, around evolved stars, and in supernova remnants. We are imaging about 180 square degrees along the Galactic Plane ($6^{\circ}$ < l < $65^{\circ}$;$-1.5^{\circ}$ < b < $+1.5^{\circ}$) with the narrow band filter centered on the [Fe II] $1.64{\mu}m$ line using WFCAM at UKIRT. The observations will complement the UWISH2 survey, which have imaged the same area with the narrow band filter centered on the molecular hydrogen 1-0 S(1) emission line at $2.12{\mu}m$, and probe a dynamically active component of ISM. We present the goals and preliminary results of our survey.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.68.1-68.1
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• 2015

Gravitational collapse is a dynamical process associated with star formation. One observational evidence of such infall motion is so called "blue asymmetry" profile, which is the optically thick line profile with the intensity peak skewed blueward relative to the intensity peak of optically thin lines. We analyzed both HCN J=1-0 and HNC J=1-0 line profiles to study the inflow motion in different evolutionary stages of massive star formation; Infrared dark clouds (IRDCs), High-mass protostellar object (HMPOs), and Ultra-compact HII regions (UCHIIs). The infall asymmetry in the HCN spectra seems to be more prevalent than the HNC spectra throughout all the three evolutionary phases. The prevalence of the blue profile in the HCN spectra is found in every evolutionary stage, with IRDCs showing the largest blue excess. In the case of the HNC spectra, only IRDCs show the blue excess statistically significant. These results suggest that HCN may be a better infall tracer in massive star forming region. In addition, even though the characteristics of the blue profile largely depend on the suitable combination of optical depth and critical density, our analyses also indicate that IRDCs may have the most active infall process compared to other evolutionary phases.

• Journal of the Korean Society of Visualization
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• v.3 no.1
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• pp.3-19
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• 2005

Novel optical techniques are presented for three-dimensional tracking of nanoparticles; Optical Serial Sectioning Microscopy (OSSM) and Ratiometric Total Internal Reflection Fluorescent Microscopy (R-TIRFM). OSSM measures optically diffracted particle images, the so-called Point Spread Function (PSF), and dotermines the defocusing or line-of-sight location of the imaged particle measured from the focal plane. The line-of-sight Brownian motion detection using the OSSM technique is proposed in lieu of the more cumbersome two-dimensional Brownian motion tracking on the imaging plane as a potentially more effective tool to nonintrusively map the temperature fields for nanoparticle suspension fluids. On the other hand, R-TIRFM is presented to experimentally examine the classic theory on the near-wall hindered Brownian diffusive motion. An evanescent wave field from the total internal reflection of a 488-nm bandwidth of an argon-ion laser is used to provide a thin illumination field of an order of a few hundred nanometers from the wall. The experimental results show good agreement with the lateral hindrance theory, but show discrepancies from the normal hindrance theory. It is conjectured that the discrepancies can be attributed to the additional hindering effects, including electrostatic and electro-osmotic interactions between the negatively charged tracer particles and the glass surface.