• Parasites, Hosts and Diseases
• /
• v.59 no.6
• /
• pp.557-564
• /
• 2021

Macrophages play a key role in chronic inflammation, and are the most abundant immune cells in the tumor microenvironment. We investigated whether an interaction between inflamed prostate cancer cells stimulated with Trichomonas vaginalis and macrophages stimulates the proliferation of the cancer cells. Conditioned medium was prepared from T. vaginalis-infected (TCM) and uninfected (CM) mouse prostate cancer (PCa) cell line (TRAMP-C2 cells). Thereafter conditioned medium was prepared from macrophages (J774A.1 cell line) after incubation with CM (MCM) or TCM (MTCM). When TRAMP-C2 cells were stimulated with T. vaginalis, protein and mRNA levels of CXCL1 and CCL2 increased, and migration of macrophages toward TCM was more extensive than towards CM. Macrophages stimulated with TCM produced higher levels of CCL2, IL-6, TNF-α, their mRNAs than macrophages stimulated with CM. MTCM stimulated the proliferation and invasiveness of TRAMP-C2 cells as well as the expression of cytokine receptors (CCR2, GP130, CXCR2). Importantly, blocking of each cytokine receptors with anti-cytokine receptor antibody significantly reduced the proliferation and invasiveness of TRAMP-C2 cells. We conclude that inflammatory mediators released by TRAMP-C2 cells in response to infection by T. vaginalis stimulate the migration and activation of macrophages and the activated macrophages stimulate the proliferation and invasiveness of the TRAMP-C2 cells via cytokine-cytokine receptor binding. Our results therefore suggested that macrophages contribute to the exacerbation of PCa due to inflammation of prostate cancer cells reacted with T. vaginalis.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.4 no.5
• /
• pp.423-432
• /
• 1999

This paper proposes a 12­pulse diode rectifier system with low kVA components suitable for powering switch mode power supplies or ac/dc converter applications. The proposed 12-pulse system employs a polyphase transformer, a zero sequence blocking transformer (ZSBT) in the dc link, and an interphase transformer. Results produce near equal leakage inductance in series with each diode rectifier bridge ensuring equal current sharing and performance improvements, The utility input currents and the voltage across the ZSBT are analyzed the kVA rating of each component in the proposed system is computed. The 5th , 7th , 17th and 19th harmonics are eliminated in the input line currents resulting in clean input power. The dc link voltage magnitude generated by the proposed rectifier system is nearly identical to a conventional to a conventional 6-pulse system. The proposed system is suitable to retrofit applications as well as in new PWM drive systems. Simulation and experimental results from a 208V , 10kVA system are shown.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.40 no.10
• /
• pp.8-15
• /
• 2003

In this paper, we address how to provide absolute differentiated services for optical burst switching (OBS) networks based on dynamic wavelength assignment. Unlike existing quality of service (QoS), such as buffer-based and offset-time based scheme, our proposed dynamicvirtual lambda partitioning (DVLP) scheme does not mandate any buffer or extra offset time, but can achieve absolute service differentiation between classes. This new DVLP scheme shares wavelength resources based on several different priority of classes in an efficient and QoS guaranteed manner. The performance results show that the proposed scheme effectively guarantees a target blocking probability of each traffic classes both in Poisson and Self-similar traffic environment.

• Proceedings of the Acoustical Society of Korea Conference
• /
• 1998.06e
• /
• pp.223-226
• /
• 1998

In the Input Buffer Switch using the intial stage FIFO memory structure, It has pointed the Throughput limitation to the percent of 58.6 due to HOL(Head of Line) blocking in the DBP(Dedicated Buffer with Pointer) method, During that time, To overcome these problems, The prior papers have proposed the complicated Arbitration algorithms and Non-FIFO memory structures. and These showed the improved Throughput. But, Now, To design high speed ATM Switch which need to the tens of Giga bit/s or the tens of Tera bit/s. It has more difficulty in proceeding the priority of majority and the complicated Cell Scheduling, because of the problem in operating the control speed of the ratio of N to scanning each port and scheduling the Cell. In this paper, To overcome these problems, We could show more the improved performance than the existing DBP Window policy to design high speed ATM Switch.

• Proceedings of the KIEE Conference
• /
• 2006.07a
• /
• pp.163-164
• /
• 2006

A current differential relay has been used for transmission line protection. The relay may maloperate in the case of a failure of the secondary circuit of a current transformer (CT) because the differential current is produced. This paper presents an algorithm to detect a failure of a CT using the zero-sequence current. If the magnitude of the zero-sequence current is the same as the magnitude of the current of the other healthy phases, a failure of a CT is detected and then the blocking signal is activated. The proposed algorithm prohibit the maloperation of a differential relay in the case of a CT failure and thus increase the security of the relay.

• YAKHAK HOEJI
• /
• v.52 no.1
• /
• pp.48-55
• /
• 2008

CD29 $({\beta}1-integrins)$ is one of major adhesion molecules involved in regulating cell adhesion, migration and morphological changes. In this study, we investigated the regulatory role of CD29 in monocytic functions using monocytic cell line U937 cells. CD29 was found to be one of highly expressed membrane proteins in U937 cells, according to flow cytometric analysis. The activation of CD29 by agonistic antibody MEM101A and extracellular matrix protein (ECM) fibronectin strongly induced cell-cell and cell-fibronectin adhesions. However, blocking antibodies to CD98 and CD147 showed different inhibitory features in these two adhesion events. Furthermore, U0126, an ERK inhibitor, only blocked cell-cell adhesion but not cell-fibronectin adhesion, indicating that cell-cell or cell-fibronectin adhesion events may be regulated by different molecular mechanisms. Meanwhile, CD29 activation also enhanced ROS generation but not phagocytic ability, and similarly radical scavenger N-acetyl-L-cysteine strongly blocked CD29-mediated cell-cell adhesion, implying that ROS may play a critical role in up-regulating cell-cell adhesion. Therefore, our data suggest that the activation of CD29 may be critically involved in regulating monocytic cell-mediated cell-cell adhesion and ROS generation.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.1
• /
• pp.495-498
• /
• 2013

Epidermal growth factor receptor (EGFR) is over-expressed in several human cancers. This would suggest that inhibition of EGFR is a reasonable approach for cancer treatment. In this study we investigated EGFR blocking and its effects on the mediated signaling such as MAPK and STATb in HT29 cells. For this aim we used FITC-labeled EGFR antisense oligonucleotides encapsulated with PAMAM nanoparticles to inhibit EGFR expression. Cellular uptake of antisense was investigated by fluorescence microscopy and flow cytometry analysis. The effect of EGFR antisense on the expression of EGFR in HT29 cells was examined by real time PCR and Western blots, which showed that antisense encapsulated with PAMAM decreased the level of EGFR mRNA and protein. In addition, real time PCR results confirmed that EGFR inhibition had an effective role in the reduction of EGFR dependent downstream genes. In conclusion, EGFR antisense encapsulated with PAMAM nanoparticles down regulated EGFR and EGFR-mediated genes.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.49 no.2
• /
• pp.137-145
• /
• 2016

This study screened the biological activity of an acidified gill extract of the Manila clam Ruditapes philippinarum including antimicrobial, hemolytic, membrane permeabilization, and DNA-binding activity, and purified the antimicrobial material. The acidified gill extract showed potent antimicrobial activity against Bacillus subtilis and Escherichia coli without significant hemolytic activity, but showed no membrane permeabilization or DNA-binding ability. An antimicrobial material was purified from the acidified gill extract using C₁₈ reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). Treatment of the purified material with trypsin completely abolished all of the antibacterial activity against Bacillus subtilis, suggesting that the purified material is a proteinaceous antibiotic. The molecular weight of the purified material was 2571.9 Da, but no primary structural information was obtained due to N-terminal blocking. A future study should confirm the primary structure. Our results suggest that the Manila clam gill contains proteinaceous antibiotics that have a role in first-line defense. This information could be used to better understand the Manila clam innate immune system.

• Biomolecules & Therapeutics
• /
• v.22 no.4
• /
• pp.282-287
• /
• 2014

We show that silymarin, a polyphenolic flavonoid isolated from milk thistle (Silybum marianum), inhibits cytokine mixture (CM: TNF-${\alpha}$, IFN-${\gamma}$, and IL-$1{\beta}$)-induced production of nitric oxide (NO) in the pancreatic beta cell line MIN6N8a. Immunostaining and Western blot analysis showed that silymarin inhibits iNOS gene expression. RT-PCR showed that silymarin inhibits iNOS gene expression in a dose-dependent manner. We also showed that silymarin inhibits extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) phosphorylation. A MEK1 inhibitor abrogated CM-induced nitrite production, similar to silymarin. Treatment of MIN6N8a cells with silymarin also inhibited CM-stimulated activation of NF-${\kappa}B$, which is important for iNOS transcription. Collectively, we demonstrate that silymarin inhibits NO production in pancreatic beta cells, and silymarin may represent a useful anti-diabetic agent.

• Journal of the Society of Naval Architects of Korea
• /
• v.47 no.1
• /
• pp.93-98
• /
• 2010

In this paper, PLC which can be installed easily and is stable to transfer datum, is tested for approving its application in a real ship. Internet access service, CCTV monitoring, light control and huge sensor communications are needed for enhancing the convenience and safety of passengers and crew in ships. In order to apply PLC in ships, we surveyed some noises interrupting PLC in ships and investigated the PLC characteristics. The Hannara, a training ship of Korea Maritime University, was used for the test. We measured and analyzed blocking noises using by NI's SCXI-1001. When noises of specific frequency band occurred near the PLC frequency band, PLC transfer capacity was low for mass datum. We developed and verified some methods to apply PLC in a ship under lots of noises.