• Journal of Microbiology
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• v.35 no.3
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• pp.177-180
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• 1997

The purpose of this study was to evaluate the efficiency of Line Probe Assay (LiPA) in detecting the rpoB gene mutation of clinically isolated Mycobacterium tuberculosis (MTB) and to compare the level of resistance to the various rifamycins with their mutation sites. The mutation in the rpoB gene was found in 84 (97.6%) out of 86 rifampicin (RMP) resistant strains as determined by LiPA. No mutation was observed in 2 RMP resistant strains and in any of 38 RMP susceptible strains tested. Only one of 3 strains with .DELTA.5/R5, one of 2 strains with .DELTA.3, and one of 3 strains with .DELTA.2/R2 LiPA profile showed a slightly lower level of resistance to the rifapentine than the other strains. Although we could not find correlations between mutation sites in the rpoB gene and the level of susceptibility to the various rifamycins, the LiPA is recommended as a fast screening tool for detection of RMP resistant MTB.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.11
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• pp.61-68
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• 2004

Recently, there has been an increasing demand for computer-vision based inspection and/or measurement system as a part of factory automation equipment. Existing manual inspection method can inspect only specific samples and has low measuring accuracy as well as it increases working time. Thus, in order to improve the objectivity and reproducibility, computer-aided analysis method is needed. In this paper, front and side profile inspection and/or data transfer system are developed using computer-vision during the inspection process on three kinds of pipes coming from a forming line. Straight line and circle are extracted from profiles obtained from vision using Laplace operator. To reduce inspection time, Hough Transform is used with clustering method for straight line detection and the center points and diameters of inner and outer circle are found to determine eccentricity and whether good or bad. Also, an inspection system has been built that each pipe's data and images of good/bad test are stored as files and transferred to the server so that the center can manage them.

• The KIPS Transactions:PartD
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• v.17D no.6
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• pp.463-470
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• 2010

We presented a very fast and robust method of text line segmentation based on the DCT blocks of color image without decompression and binary transformation processes. Using DC and another three primary AC coefficients from block DCT we created a gray-scale image having reduced size by 8x8. In order to detect and locate white strips between text lines we analyzed horizontal and vertical projection profiles of the image and we applied a direct markov model to recover the missing white strips by estimating hidden periodicity. We presented performance results. The results showed that our method was 40 - 100 times faster than traditional method.

• Journal of the Korean Geophysical Society
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• v.3 no.1
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• pp.67-74
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• 2000

Subsurface structure of the Bomun basin was studied along three survey tracks of Line-1, Line-2, and Line-3 using geomagnetic, radioactive, and seismic refraction methods. Abrupt changes found at 2.55 km west and 1.6 km east in the profile of magnetic anomaly along Line-1 are correlated with geologic boundary of the basin. Profiles of radioactive intensity also represent abrupt changes at 2.55 km west of Line-1 and at 1.9 km of Line-2. Cretaceous basement rock has relatively high magnetic anomaly of $200\;{\sim}\;500\;nT$ while sedimentary rocks of the Bomun basin have relatively low magnetic anomaly of $-100\;{\sim}\;+100\;nT$. Radioactive intensity also represents charateristic differences between Cretaceous basement and sedimentary rocks of the Bomun basin. Rocks of Cretaceous basement have lower radioactive intensity than the rocks of the Bomun basin. Magnetic anomaly of of the Bomun basin represents lowest anomaly in western part and increases gradullay toward east. This phenomenon is interpreted as a half graben structure dipping westward. Black shale known by previous studies near the western boundary has high magnetic anomalies and low radioactive intensity. This phenomenon provide a possibility of volcanic rock rather than black shale near the western boundary of the basin along Line-1. Sedimentary layers having velocities of 455 m/s, 1904 m/s, and 2662 m/s are developed to have westward dipping of $2.3^{\circ}$ in the central area of the Bomun basin. The result is consistent with a half-graben model dipping westward which were derieved from magnetic anomaly data.

• Journal of Environmental Science International
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• v.13 no.3
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• pp.245-250
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• 2004

The SBR(Sequencing Batch Reactor) process is ideally suited to treat high loading wastewater due to its high dilution rate. SBR operates by a cycle of periods consisting of filling, reacting, settling, decanting and idling. The react phases such as aeration or non-aeration, organic oxidation, nitrification, denitrification and other bio-logical reactions can be achieved in a reactor. Although the whole reactions can be achieved in a SBR with time distributing, it is hard to manage the SBR as a normal condition without recognizing a present state. The present state can be observed with nutrient sensors such as ${NH_{4}}^{+}-N$, ${NO_{2}}^{-}-N$, ${NO_{3}}^{-}-N} and ${PO_{4}}^{ 3-}-P.$ However, there is still a disadvantage to use the nutrient sensors because of their high expense and inconvenience to manage. Therefore, it is very useful to use common on-line sensors such as DO, ORP and pH, which are less expensive and more convient. Moreover, the present states and unexpected changes of SBR might be predicted by using of them. This study was conducted to get basic materials for making an inference of SBR process from ORP(oxidation reduction potential) of synthetic wastewater. The profiles of ORP, DO, and pH were under normal nitrification and denitrification were obtained to compare abnormal condition. And also, nitrite and nitrate accumulation were investigated during reaction of SBR. The bending point on ORP profile was not entirely in the low COD/NOx ratio condition. In this case, NOx was not entirely removed, and minimum ORP value was presented over -300mV. Under suitable COD/NOx ratio which complete denitrification was achieved, ORP bending point was observed and minimum ORP value was under -300m V. Under high COD/NOx ratio, ORP bending point was not detected at the first subcycle because of the fast denitrification and minimum ORP value was under -300mV at the time.

• Journal of Acupuncture Research
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• v.24 no.1
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• pp.151-163
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• 2007

Objectives : Juglandis Semen herbal acupuncture solution(JSS) has a broad array of clinical applications in oriental medicine, including treatment of chronic musculoskeletal diseases such as arthritis. This study was performed to investigate the global gene expression profiles using microarray assay in RAW 264.7 cell line treated with JSS and to advance our understanding of the pharmacologic effect of JSS. Methods : Change of the gene expression profile in RAW cell line following treatment with lipopolysaccharide(LPS) alone, or with LPS plus JSS was investigated with a cut-off level of 2 fold change in the expression. Especially, Change of the gene expression by treatment with LPS alone was compared with that by treatment with LPS plus JSS with a cut-off level of 1/2 fold change in the expression. Results: Of the 8170 genes profiled in this study, 51 were upragulated and 21 downregulated following LPS treatment, and 88 were upregulated and 69 downregulated following costimulation of JSS and LPS. Of the 51 genes upregulated following LPS treatment, 10 were downregulated following costimulation of JSS and LPS. Of the 21 genes downregulated following LPS treatment, 3 were upregulated following costimulation of JSS and LPS. Conclusion : JSS treatment induced upregulation of some genes including IL-10 and downregulation of that including MMP13 with its possible implication in an antiinflammatory action of JSS. However, further research on expression profile changes induced by JSS treatment is expected.

• Molecular & Cellular Toxicology
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• v.5 no.1
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• pp.51-57
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• 2009

Quantum Dot (QD) nanoparticles are used in various industrial applications, such as diagnostic, drug delivery, and imaging agents of biomedicine. Although QDs are extensively used in many medical science, several studies have been demonstrated the potential toxicity of nanoparticles. The first objective of this study was to investigate the nanotoxicity of QDs in the HaCaT human keratinocyte cell line by focusing on gene expression pattern. In order to evaluate the effect of QDs on gene expression profile in HaCaT cells, we analyzed the differential genes which related to oxidative stress and antioxidant defense mechanisms by using human cDNA microarray and PCR array. A human cDNA microarray was clone set, which was sorted for a list of genes correlated with cell mechanisms. We tried to confirm results of cDNA microarray by using PCR array, which is pathway-focused gene expression profiling technology using Real-Time PCR. Although we could not find the exactly same genes in both methods, we have screened the effects of QDs on global gene expression profiles in human skin cells. In addition, our results show that QD treatment somehow regulates cellular pathways of oxidative stress and antioxidant defense mechanisms. Therefore, we suggest that this study can enlarge our knowledge of the transcriptional profile and identify new candidate biomarker genes to evaluate the toxicity of nanotoxicology.

• Computers and Concrete
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• v.1 no.4
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• pp.389-400
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• 2004

The examinations carried out have confirmed a relationship existing between the character of fracture surfaces and the composition and structure of (basalt and gravel) concretes. For both concretes investigated, a very good correlation was obtained between the profile line development factor, $R_L$, and the fracture surface development factor, $R_S$. With the increase in the $R_L$ parameter, the fracture surface development factor $R_S$ also increased. Agreement between the proposed relationship of $R_S=f(R_L)$ and the proposal given by Coster and Chermant (1983) was obtained. Stereological examinations carried out along with fractographic examinations made it possible to obtain a statistical model for the determination of $R_L$ (or $R_S$) based on the volume of air voids in concrete, $V_{air}$, the specific surface of air pores, $S_V_{air}$ the specific surface of coarse aggregate, $S_{Vagg.}$, and the volume of mortar, $V_m$. An effect of coarse aggregate type on the obtained values of the profile line development factor, $R_L$, as well as on the relationship $R_S=f(R_L)$ was observed. The increment in the fracture surface development factor $R_S$ with increasing $R_L$ parameter was larger in basalt concretes than in gravel concretes, which was a consequence of the level of complexity of fractures formed, resulting chiefly from the shape of coarse aggregate grains.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.11 s.114
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• pp.1058-1064
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• 2006

In this paper, the channel capacity of a specified wireless indoor multiple-input multiple-output(MIMO) channel is estimated by analyzing spatial characteristics of this channel using the three-dimensional ray tracing method, and a technique for deriving an optimized separation of multi-antenna elements is proposed. At first, the ray paths, the path losses, and the time-delay profile are computed using the three-dimensional ray tracing method in an indoor corridor environment, which has the line of sight(LOS) and non-line of sight(NLOS) regions. The ray tracing method is verified by a comparison between the computation results and the measurements which are obtained with dipole antennas, an amplifier and a network analyzer. Then, an MIMO system is positioned in the indoor channel environment and the ray paths and path losses are computed for four antenna-position combinations and various values of the antenna separation to obtain the channel capacity for the MIMO system. An optimum antenna-separation is derived by averaging the channel capacities of 100 receiver positions with four different antenna combinations.

• The Journal of Korean Obstetrics and Gynecology
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• v.22 no.3
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• pp.117-134
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• 2009

Purpose: In the theory of traditional medicine, Scutellariae Radix (SR) can clear away heat and remove dampness, purge the sthenic fire and remove toxic materials, cool blood and stop bleeding to prevent miscarriage. Recently, SR is known to have anti-cancer activity. For this reason, the present author designed to investigate the effect of SR on proliferation rates of cervical cancer cell line, then effects on genetic profile by SR. Methods: The genetic profile for the effect of SR on human derived cervical cancer cell line, SNU-703, was measured using microarray technique, and the functional analysis on these genes was conducted. Results: Total 519 genes were up-regulated and 606 genes down-regulated in cells treated with SR. Genes induced or suppressed by SR were all mainly concerned with metabolic process, regulation of biological process and protein binding. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as TNFRSF1A, AKT1, MAPK3, and STAT3 that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conclusion: These results suggest possibility of SR as anti-cancer drug and also suggest that related mechanisms are involved in TNFRSF1A, AKT1, MAPK3, and STAT3 related signalling pathways.