• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.329-333
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• 2005

This study was designed to investigate the stability of a lipase fused with a cellulose­binding domain (CBD) to cellulase. The fusion protein was derived from a gene cluster of a CBD fragment of a cellulase gene in Trichoderma hazianum and a lipase gene in Bacillus stearother­mophilus L1. Due to the CBD, this lipase can be immobilized to a cellulose material. Factors affecting the lipase stability were divided into the reaction-independent factors (RIF), and the re­action-dependent factors (RDF). RIF includes the reaction conditions such as pH and tempera­ture, whereas substrate limitation and product inhibition are examples of RDF. As pH 10 and $50^{\circ}C$ were found to be optimum reaction conditions for oil hydrolysis by this lipase, the stability of the free and the immobilized lipase was studied under these conditions. Avicel (microcrystal­line cellulose) was used as a support for lipase immobilization. The effects of both RIF and RDF on the enzyme activity were less for the immobilized lipase than for the free lipase. Due to the irreversible binding of CBD to Avicel and the high stability of the immobilized lipase, the enzyme activity after five times of use was over $70\%$ of the initial activity.

• Korean Institute of Interior Design Journal
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• no.15
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• pp.69-78
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• 1998

Purchasing should be considered as an exciting entertainment by itself in these days. Customers can be satisfied with making their own choices and dreaming of their better lives through the action of buying,. However the customers are not rushing into stores without any special reason except when the store has something attracts them. Generally speaking the customers will willingly spend their time and effort in shopping only when they are attracted by well-displyed goods and reasonably planned layout. Based upon these concepts interior design as a creative marketing tool plays a great role in enhancing customer's circulation and the productivity of a store. This study is to propose the layouts which are increasing the customer's circulation by contacting the customers with commodities as many as possible. This study is primarily revolved around the parts affecting customer's circulation among the factors of store's layouts. This study was consist of theoretical consideration about the store's layouts on the foundation of conference and existing works and several proposals. The results of this research are as below: 1) The entrance and exit of a retail store should be apart from each other to increase customers' circulation. It is regarded as ideal to locate the entrance on the right side of front to control moving line of customers. 2) A shop should be surrounded with the main aisles which is straightened inward from the main entrance. 3) Placing magnets which can be anything that attracts customers' attention at the corner of store can lead customers inward and increase customers' circulation.

• Journal of the Ergonomics Society of Korea
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• v.29 no.3
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• pp.321-329
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• 2010

Unlike production line works, construction work is non-repetitive in nature. In this study, a checklist was developed to characterize the ergonomic hazards of construction work. Eight existing checklists including OWAS, RULA, and REBA were integrated, and a new version was designed to include more postures of lower extremities. The analysis results obtained using the new checklist showed that manual materials handling (MMH) is the most common risk factor. About 62% of the MMH was done below the knee level or above the elbow level, and 22% was carried out with the trunk twisted more than $60^{\circ}$. The next risk factors were the works done with the knee bent or squatted, or with the shoulders abducted. It can be concluded that the new checklist is successful as a quick and easy tool for screening risk factors of construction works, although it is not capable of determining action levels. Further studies on validation of the checklist are expected.

• BMB Reports
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• v.40 no.6
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• pp.944-951
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• 2007

3-Hydrogenkwadaphnin (3-HK) is a daphnane-type diterpene ester isolated from Dendrostellera lessertii (Thymelaeaceae) with high differentiation and apoptotic potency in leukemic cells without any measurable adverse effects on normal cells (Moosavi et al., 2005b). In this study, we report that 3-HK (12 nM) has the ability to cease proliferation, induce differentiation and apoptosis in chronic myelogenous leukemia (CML) K562 cell line. The treated cells lost erythroid properties and differentiated along the megakaryocytic lineage based on the morphological features apparent after Wright-Giemsa staining, DNA content analysis and the expression of cell surface marker glycoprotein IIb as analyzed by flow cytometry. Moreover, using Hoechst 33258 and Annexin V double staining indicated the occurrence of apoptosis among the treated cells. On the other hand, restoration of the depleted GTP pool size by exogenous addition of guanosine ($50{\mu}M$) reduced the effect of the drug regarding the extent of differentiation while no further enhancement of 3-HK effect was obtained by addition of exogenous hypoxanthine ($100{\mu}M$). These interesting results necessitate further investigation regarding the mechanism of action of this unique anti-leukemic agent.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.354-361
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• 2005

Vibrio fluvialis, an enteropathogenic bacterium, produces a phospholipase which is thought to be an important factor in the pathogenesis of disease. In this study, the phospholipase gene (vfp) was identified from V fluvialis (KCTC 2473) and its sequence was determined. The entire open reading frame was composed of 1,689 nuc1eotides and 563 amino acids. The phospholipase gene (vfp) was overexpressed in Escherichia coli as a his-tag fused protein. This recombinant protein (rVFP58) was solubilized with 6 M urea and purified by Ni-NTA affinity chromatography. The action mode of rVFP58 was determined by TLC and GC-MS, and it showed phospholipase B activity, which had both phospholipase A and lysophospholipase activities. The rVFP58 showed a maximum activity at pH around 9- 10 and temperature of about 40OC, and it was stable under alkaline condition over pH 9. The cytotoxicity of rVFP58 was evaluated, using a fish cell line, CHSE-2l4, and was found to cause significant cell death after 14 h of exposure to 250 $\mu$g of the protein.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.360-367
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• 1994

The effect of various nutrients for the production of the anti-complementary polysaccha- ride by Flammulina velutipes, was examined and the partial purification of the polysaccharide was carried out. The culture conditions and medium compositions considerably influenced the anti-complementary activity of the polysaccharides. When a culture was carried out at 23$\circ$C and pH 5.5 for 6 days in the synthetic medium supplemented with galactose and NaNO$_{3}$, the production of the anti-complementary polysaccharide was maximized. The crude polysaccharide, FV-1 was isolated from the culture broth and partially purified into two fractions, FV-1-II and FV-1-III by gel filtration using Sephadex G-100. FV-1-II was mainly consisted of xylose, mannose, galactose and glucose, and rhamnose, mannose and galactose, for FV-1-III. Also, the anti-complementary activity of FV-1-III was reduced partially in the absence of the Ca ion. When crossed immunoelect- rophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the FV-1-III in the Ca ion free condition, a cleavage of C3 precipitin line was observed. These results indicate that the mode of complement activation by polysaccharide from Flammulina velutipes is via not only the classical pathway but also the alternative pathway.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.47
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• pp.239-260
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• 2010

The purpose of this study would like to investigate the critical success factors of the e-procurement which means on-line purchase of goods and services for organizations. This action represents one of the largest expense items in a firm's cost structure. In order to draw CSFs of e-procurement system, we have surveyed questionnaire to the related firms. The basic variables used in this survey are taken from many precedent researches in relation to the e-procurement. On the other hand, the research data is collected from companies which had used the MRO e-marketplace. Through factor analysis of those data, four-factor resulted as follows: (1) factor one suggests the rationalization of the firm's management of its suppliers; (2) factor two calls for redesigning affected business processes and influencing end-user/employee procurement-related behaviors; (3) factor three refers to carefully orchestrating an e-procurement technology planning process with one's suppliers and using intelligence in designing the software and mining the data it produces; and (4) factor four relates to selecting an e-procurement solution and/or simultaneously participating in a number of electronic environments supporting e-procurement. In conclusion, these four factors are regarded as critical success factors for the application of e-procurement system and then suppliers should take care the effective planning of e-procurement structure.

• Structural Engineering and Mechanics
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• v.17 no.5
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• pp.655-670
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• 2004

This paper aims to evaluate the effect of typhoons on fatigue damage accumulation in steel decks of long-span suspension bridges. The strain-time histories at critical locations of deck sections of long-span bridges during different typhoons passing the bridge area are investigated by using on-line strain data acquired from the structural health monitoring system installed on the bridge. The fatigue damage models based on Miner's Law and Continuum Damage Mechanics (CDM) are applied to calculate the increment of fatigue damage due to the action of a typhoon. Accumulated fatigue damage during the typhoon is also calculated and compared between Miner's Law and the CDM method. It is found that for the Tsing Ma Bridge case, the stress spectrum generated by a typhoon is significantly different than that generated by normal traffic and its histogram shapes can be described approximately as a Rayleigh distribution. The influence of typhoon loading on accumulative fatigue damage is more significant than that due to normal traffic loading. The increment of fatigue damage generated by hourly stress spectrum for the maximum typhoon loading may be much greater than those for normal traffic loading. It is, therefore, concluded that it is necessary to evaluate typhoon induced fatigue damage for the purpose of accurately evaluating accumulative fatigue damage for long-span bridges located within typhoon prone regions.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.4
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• pp.129-135
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• 1996

The area of gear vibration and noise, has recently been the focus of many studies. The proper kinematic and geometric design of gears, the mathematical modeling of gear system are essential for a good design. This work present a gear disign for reducing noise, and practical approaches used for machinery noise reduction slong with the summary of methods available for predicting gear noise in terms of the transmis- sion error, and show a comparative study with other methods. A new tooth profile modification is proposed for reducing vibration and noise of involute gears. The method is based on the use of cubic spline curves. The tooth profile is constrained to assume an involute shape during the loaded operation. Thus the new gear profile assures conjugate motion at all points along the line of action. The new profile is found to result in a more uniform static transmission error compared to not only standard involute profile but also modificated profile therby contributing to the improvement of vibration and noise characteristics of the gear.

• Safety and Health at Work
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• v.6 no.2
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• pp.146-150
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• 2015

Occupational health and safety (OHS) is a broad field of professional practice, which involves specialists from different disciplines including but not limited to engineers, occupational health physicians, physical and biological scientists, economists, and statisticians. The preventive systems required to ensure workers are protected from injuries and illnesses dwell heavily on engineers; however, the extent to which the engineer can go regarding planning and implementing preventive measures is dependent on specific legal requirements, leadership commitment from the company, organization, and nation. The objective of this paper is to identify the areas of opportunities for improvements in OHS management in Ghana with regard to the nation's legal requirements, commitment of the Ghana government, and Ghanaian leadership as well as appropriate structuring of Ghanaian institutions responsible for monitoring and managing OHS in Ghana. This paper identified Ghana's fragmented legal requirements concerning OHS, which are under different jurisdictions with unclear responsibilities and accountabilities. The paper also highlights the training needs of Ghanaian academic institutions regarding OHS. Among other recommendations made including structuring of Ghanaian institutions to manage OHS in line with the ILO-OSH 2001, this paper aligns the recommendations with the articles and elements of International Labour Organization convention number 155 and OHSAS 18001 elements.