• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.1
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• pp.1-5
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• 1998

One cervical cancer cell line, C9, carrying human papillomavirus type 18 (HPV18) genes that is one of the major etiologic concoviruses for cervical cancer was characterized. This cell line was further characterized for its capacity related to the epithelial cell proliferation, stratification and differentiation in reconstituted artificial epithelial tissue. The in vitro construction of three dimensional artificial cervical opithelial tissue has been engineered using C9 epithelial cancer cells, human foreskin fibroblasts and a matrix made of type I collagen by organotypic culture of epithelial cells. The morphology of paraffin embedded artificial tissue was examined by histochemical staining. The artificial epithelial tissues were well developed having multilayer. However, the tissue morphology was similar to the cervical tissus having displasia induced by HPV infection. The characteristics of the artificial tissues were examined by determinining the expression of specific marker proteins. In the C9 derived artificial tissues, the expression of EGF receptor, as epithelial proliferation marker proteins for stratum basale was observed up to the stratum spinosum. Another epithelial proliferation marker for stratum spinosum, cytokerations 5/6/18, were observed well over the stratum spinosum. For the differentiation markers, the expression of involucrin and filaggrin were observed while the terminal differentiation marker, cytokeratins 10/13 was not detected at all. Therefore the reconstituted artificial epithelial tissues expressed the same types of differentiation marker proteins that are expressed in normal human cervical epithelial tissues but lacked the final differentiation capacity representing characteristics of C9 cell line as a cancer tissue devived cell line. Expression of HPV18 E6 oncoprotein was also observed in this artifical cervical opithelial tissue though the intensity of the staining was weak. Thus this artificial epithelial tissue could be used as a useful model system to examine the relationship between HPV-induced cervical oncogenesis and epithelial cell differentiation.

• Biomolecules & Therapeutics
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• v.29 no.5
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• pp.498-505
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• 2021

Amyotrophic lateral sclerosis (ALS) is a lethal neurological disorder characterized by the deterioration of motor neurons. The aim of this study was to investigate alteration of cationic amino acid transporter (CAT-1) activity in the transport of lysine and the pretreatment effect of lysine on pro-inflammatory states in an amyotrophic lateral sclerosis cell line. The mRNA expression of cationic amino acid transporter 1 was lower in NSC-34/hSOD1^G93A (MT) than the control cell line (WT), lysine transport is mediated by CAT-1 in NSC-34 cell lines. The uptake of [³H]L-lysine was Na⁺-independent, voltage-sensitive, and strongly inhibited by inhibitors and substrates of cationic amino acid transporter 1 (system y⁺). The transport process involved two saturable processes in both cell lines. In the MT cell line, at a high-affinity site, the affinity was 9.4-fold higher and capacity 24-fold lower than that in the WT; at a low-affinity site, the capacity was 2.3-fold lower than that in the WT cell line. Donepezil and verapamil competitively inhibited [³H]L-lysine uptake in the NSC-34 cell lines. Pretreatment with pro-inflammatory cytokines decreased the uptake of [³H]L-lysine and mRNA expression levels in both cell lines; however, the addition of L-lysine restored the transport activity in the MT cell lines. L-Lysine exhibited neuroprotective effects against pro-inflammatory states in the ALS disease model cell lines. In conclusion, studying the alteration in the expression of transporters and characteristics of lysine transport in ALS can lead to the development of new therapies for neurodegenerative diseases.

• 전기의세계
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• v.18 no.1
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• pp.24-29
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• 1969

This is preliminary study concerning the future construction and operation of 345KV power transmission line in Korea which will be added to the existing 161KV power system by the end of 1970 in order to increase the power carrying capacity between Seoul and Pusan area; 350 kilo meters aparts, in accordance with the ambitious second five years power development schedule of Korea Electric Company. The result of this study says that an intermidiate switching station should be installed at the middle position of the line to improve the transient stability of the system, considerable amount of capacitors or synchronous condenser are to be installed to reduce the voltage drop at receving end of line during the heavy load hours, and also in some measure to avoid the voltage rise by self-excitation of power generators during the light load hours and while energizing the line. This is the first attempt to realize the EHV power transmission line in Korea so that the additional study is necessary on the kind and size of conductors, the necessary number of insulators and the suitable clearance distances between conductor and steel tower or earth from the technical and economical view points. These are necessary steps to be taken by the writer before getting into the calculation on the transient stablity of the power system.

• Progress in Superconductivity and Cryogenics
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• v.7 no.1
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• pp.37-41
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• 2005

This paper describes the construction scheme of new distribution system using HTS(High Temperature Superconducting) power equipments such as cable, transformer and FCL(fault current limiter). At present, one of the most serious problems in distribution power system, especially for metropolitan complex city, is to obtain the ROW for cable line routes, space for downtown substations and satisfy the environmental protection caused by NIMBY phenomena. Unfortunately, it is expected that this situation will get more and more worse. As the HTS technology to apply in power system Is developed, HTS cable utilizing mass-capacity characteristic can be a useful countermeasure to overcome this problem. This paper describes the application methodology of 22.9kV HTS cable with low-voltage, mass-capacity characteristics replacing the 154kV conventional cable. By applying 22.9kV HTS cable, the HTS transformer with higher capacity for the reduction of space and transformer numbers of downtown substation is necessary. Also, if the leakage Impedance of HTS transformer is same as or lower than that of conventional transformer, the fault current of 22.9kV bus will increase because the HTS transformer capacity is larger than that of the conventional transformer. This means the parallel application of HTS-FCL to reduce the fault current in addition to the HTS cable and transformer can be necessary. With the basic construction scheme of new distribution system, this paper describes the future study points to realize this new distribution system using HTS equipments.

• Steel and Composite Structures
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• v.20 no.3
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• pp.487-500
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• 2016

In order to investigate the flexural bearing capacity of panel zone of diaphragm-through joint between concrete filled square steel tubular column and steel beam, four specimens were tested under static tension loads to study the mechanical properties and bearing capacity of diaphragm-through joints with a failure mode of panel zone. Finite element models of these specimens were developed to simulate the test and compare the predicted failure modes, load-displacement curves and bearing capacities with the experimentally observed. It was found that the tensile load from the steel beam flange is mainly shared by the square steel tube and the diaphragm. The diaphragm plastic zone appears along the cross-section lines enclosed by the square steel tube and the influence of steel beam web on the plastic zone of the steel tube is significant and cannot be neglected. Computational models of yield lines on square steel tube and diaphragm are established based on the distribution pattern of the plastic zone, and an analytical method for the evaluation of the bearing capacity of the joint is proposed. The theoretical results and the experimental data are compared and found in good agreement.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.55 no.9
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• pp.478-484
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• 2006

The paper presents methods of determining the capacity and installation positions of regenerative inverters installed in DC 1500V electric railway system. We suggested a method that approximates using parameters related to substations where regenerative inverters are installed, railway lines and operating motor cars, and another that calculates using regenerative power obtained from Train performance Simulation (TPS) and Power Flow Simulation (PFS). We carried out TPS and PFS for Seoul Subway Line $5{\sim}8$, calculating regenerative power and determining substations where regenerative inverters would be installed and the optimal capacity and number of inverters to be installed.

• Journal of the Korean Institute of Telematics and Electronics
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• v.25 no.6
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• pp.591-597
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• 1988

In this paper, we have studied a channel capacity affected by noise which produces burst errors in the ISDN(approx 140Kbps) data transmission using a existing PSTN. First, a digital communication system model to apply a subscriber line to a narrow band ISDN channel is presented. Second, the decision methodology of a channel capacity limitation whether the pdf of the noise generated by a waveform synthesizer is Pisson or Laplacian is described. As a result of the simulation, we have obtained that the Poission distributed noise is approached to the Guassian rather than the Laplacian under the same SNR condition.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.57 no.3
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• pp.302-310
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• 2008

In order to evaluate the nominal rating of breakers in distribution system due to the increased capacity and operating conditions of power transformers in 154 kV substation, the fault currents in distribution system were calculated by the conventional method and simulations of PSCAD/EMTDC program. Consequently, under the condition of the parallel operation of transformers, the fault currents exceed the nominal current of the breakers in some areas. Without NGR at the secondary neutral of the transformer, the current of single line-to-ground fault was bigger than that of 3-phase fault. Therefore, the results clearly show that the measures to limit the fault currents in distribution system are needed when the increased capacity of power transformers is introduced into 154 kV substation.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.27-28
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• 2008

For stable and sustainable energy supply, distributed generator (DG) has become an essential and indispensable element from environmental and energy security perspectives. However, installation of DG in distribution systems may cause negative affects on feeders because power outputs of DG could be changed irregularly. One of major negative affects is variation in voltage profile. In general, voltage regulation devices such as under load tap changer (ULTC) at distribution substation and step voltage regulator (SVR) along feeder in distribution system are used to maintain customers' receiving voltage within a predetermined range. These regulators are controlled by line drop compensation (LDC) method which calls for two parameters; the equivalent impedance and the load center voltage. Therefore, consideration of DG outputs in the LDC parameter design procedure may give large impact on the installable DG capacity. This paper proposes a method that estimates maximum Installable DG capacity considering LDC parameters of ULTC and SVR. The proposed algorithm is tested with model network.

• Environmental Analysis Health and Toxicology
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• v.12 no.3_4
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• pp.75-84
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• 1997

Chromatographic retention behavior and separation of various phenol derivatives on a Partisil 10 ODS 3 column-with mobile phase containing $\alpha$-cyclodextrin-were systematically studied. The decrease in k' values caused by the addition of cyclodextrins in the mobile phase was based on the formation of an inclusion complex, resulting in weakening of the hydrophobic interaction between solutes and the stationary phase. The content of the organic solvent in the mobile phase also influenced k' values of the solutes, and k' values increased with a decrease of the content of organic solvent in the mobile phase. A simple equation has been derived that reveals the hyperbolic dependence of the capacity factor on the total concentration of cyclodextrin. A plot of the reciprocal of the capacity factor against (CD)$_T$ gives a straight line and the dissociation constant, K$_D$, of the inclusion complex can be calculated from the slope. The capacity factor decreased with increasing temperature. The enthalpy was calculated from the slope of van't Hoff plots. Under optimum conditions, some mixtures of phenol derivatives were able to separated successfully.