• Title/Summary/Keyword: Limit of Detection

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SPACE-BASED OCEAN SURVEILLANCE AND SUPPORT CAPABILITY

  • Yang Chan-Su
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.253-256
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    • 2005
  • The use of satellite remote sensing in maritime safety and security can aid in the detection of illegal fishing activities and provide more efficient use of limited aircraft or patrol craft resources. In the area of vessel traffic monitoring for commercial vessels, Vessel Traffic Service (VTS) which use the ground-based radar system have some difficulties in detecting moving ships due to the limited detection range. A virtual vessel traffic control system is introduced to contribute to prevent a marine accident such as collision and stranding from happening. Existing VTS has its limit. The virtual vessel traffic control system consists of both data acquisition by satellite remote sensing and a simulation of traffic environment stress based on the satellite data, remotely sensed data. And it could be used to provide timely and detailed information about the marine safety, including the location, speed and direction of ships, and help us operate vessels safely and efficiently. If environmental stress values are simulated for the ship information derived from satellite data, proper actions can be taken to prevent accidents. Since optical sensor has a high spatial resolution, JERS satellite data are used to track ships and extract their information. We present an algorithm of automatic identification of ship size and velocity. This paper lastly introduce the field testing results of ship detection by RADARSAT SAR imagery, and propose a new approach for a Vessel Monitoring System(VMS), including VTS, and SAR combination service.

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Detection of Pathogenic Salmonella Using a Surface Plasmon Resonance Biosensor (표면플라즈몬공명 바이오센서를 이용한 살모넬라 검출)

  • Cho, Han-Keun;Kim, Gi-Young;Kim, Woon-Ho;Sung, Min-Sun
    • Journal of Biosystems Engineering
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    • v.35 no.2
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    • pp.116-123
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    • 2010
  • Rapid detection of foodborne pathogens has been a major challenge for the food industry. Salmonella contamination is well known in all foods including pasteurised milk. The possibility of specific detection of Salmonella Enteritidis by surface plasmon resonance (SPR) biosensor was explored using a commercially available portable SPR sensor. Self assembly technique was adopted to immobilize anti-Salmonella antibodies on the gold sensing surface of the SPR sensor. The concentration of polyclonal antibody for use in the SPR biosensor was chosen to 1.0 mg/mL. Experiments were conducted at near real-time with results obtained for one SPR biosensor assay within 1 hour. The limit of detection for Salmonella Enteritidis was determined to be $10^6$ CFU/mL in both PBS buffer and milk samples. The assay sensitivity was not significantly affected by milk matrix. Our results showed that it would be possible for employing the SPR biosensor to detect Salmonella Enteritidis in near real-time.

Moving Vehicle Detection from Single-pass Worldview-3 Imagery Using Spatial Correlation Map

  • Song, Yongjun;Chung, Minkyung;Kim, Yongil
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.40 no.5
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    • pp.439-448
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    • 2022
  • MV (Moving Vehicle) detection using satellite imagery is important for traffic monitoring and provides a wide range of observations. Specifically, MV detection methods utilizing the time lag in single-pass optical satellite images have been studied for detecting MVs from a single set of images. Because of limitations in detecting MVs outside of roads, most previous studies required road information to limit the moving object to cars on the road. However, it is difficult to obtain road information from inaccessible areas. Therefore, this study proposed a new method for detecting MVs regardless of their locations from single-pass optical satellite images without using additional data. WV-3 (Worldview-3) satellite images were used, and a spatial correlation coefficient map was proposed to detect spatial displacement which denotes MVs across two WV-3 MS images. Finally, evaluation was performed through quantitative metrics and visual inspection. The evaluation results revealed that the proposed method can detect MV movements from the single-pass satellite images. On the contrary, misdetected or undetected MVs due to radiometric differences between the images could be identified by visual inspection. The performance of the proposed method can be improved by minimizing radiometric variations and adding conditions that are robust to radiometric differences between the images.

Real-Time Eye Detection and Tracking Under Various Light Conditions

  • Park Ho Sik;Nam Kee Hwan;Seol Jeung Bo;Cho Hyeon Seob;Ra Sang Dong;Bae Cheol Soo
    • Proceedings of the IEEK Conference
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    • 2004.08c
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    • pp.862-866
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    • 2004
  • Non-intrusive methods based on active remote IR illumination for eye tracking is important for many applications of vision-based man-machine interaction. One problem that has plagued those methods is their sensitivity to lighting condition change. This tends to significantly limit their scope of application. In this paper, we present a new real-time eye detection and tracking methodology that works under variable and realistic lighting conditions. Based on combining the bright-pupil effect resulted from IR light and the conventional appearance-based object recognition technique, our method can robustly track eyes when the pupils are not very bright due to significant external illumination interferences. The appearance model is incorporated in both eyes detection and tracking via the use of support vector machine and the mean shift tracking. Additional improvement is achieved from modifying the image acquisition apparatus including the illuminator and the camera.

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Development of the rapid detection kit for Salmonella spp. using immunochromatographic assay (면역크로마토그라피 기법을 이용한 Salmonella 속균 신속 검출킷트 개발)

  • Jung, Byeong-yeal;Jung, Suk-chan
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.191-197
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    • 2005
  • An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth. And then it was 10-fold diluted from $5.2{\times}10^{8}CFU/ml$ to 5.2 CFU/ml. The IC strip could detect $5.2{\times}10^{6}CFU/ml$ before enrichment. However, the lowest limit of detection was 5.2 CFU/ml after overnight incubation. The results indicated that the IC assay was a rapid, economical and simple method with high specificity and sensitivity for the detection of Salmonella spp. without using any equipment.

Fluorimetric Determination of Dichloroacetamide by RPLC with Postcolumn Detection

  • Choi, Yong-Wook;Reckhow, David A.
    • Bulletin of the Korean Chemical Society
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    • v.25 no.6
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    • pp.900-906
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    • 2004
  • An RPLC-postcolumn detection method has been developed for the fluorimetric determination of dichloroacetamide (DCAD) in water. After ammonia and DCAD were separated on a $C_{18}$ nonpolar stationary phase with 2.5% methanol-0.02 M phosphate buffer at pH 3, the column eluant was reacted with post column reagents, o-phthaldialdehyde (OPA) and sulfite ion at pH 11.5, to produce a highly fluorescent isoindole fluorophore, which was measured with a fluorescence detector ( ${\lambda}_{ex}$ = 363 nm, ${\lambda}_{em}$ = 425 nm). With the optimized conditions for RPLC and the postcolumn derivatization, the calibration curve was found to be linear in the concentration ranges of 0.5 and 20 ${\mu}$M for DCAD, and the detection limit for DCAD was 0.18 ${\mu}$M (23${\mu}$g/L). This corresponded to 18 pmol per 100 ${\mu}$L injection volume for a signal-to-noise ratio of 3, and the repeatability and reproducibility of this method were 1.0% and 2.5% for five replicate analyzes of 2 ${\mu}$M DCAD, respectively. The degradation yields DCAD to ammonia were 94 and 99%, and the percent recoveries of DCAD from 4 and 6 ${\mu}$M DCAD-spiked tap water were shown mean more than 97%.

Improving compensation method of target detection area difference between Electro-optical tracking system and radar (전자광학추적장비와 레이더 사이의 표적탐지영역의 차이보상방법 개선)

  • Yoo, Hyeong-Gon;Kwon, Kang-Hoon;Kim, Young-Kil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.12
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    • pp.3023-3029
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    • 2013
  • This is an example we generally have a variety of equipment that can detect and track the targets and track them quickly and accurately through the information exchange among each piece of equipment. These equipment have similar detection areas (FOV), but some are different due to the limit of the resolution of the equipment. In this paper, we studied the method of reducing detection time and tracking the targets automatically.

Novel Detection Protocol for Erwinia amylovora in Orchard Soil after Removal of Infected Trees

  • Sujin Song;Byeori Kim;Kwang-Pyo Kim;Eunjung Roh
    • The Plant Pathology Journal
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    • v.40 no.3
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    • pp.282-289
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    • 2024
  • Fire blight is a bacterial disease caused by Erwinia amylovora. In Korea, fire blight was first reported in 2015 in an orchard. If the infection is confirmed, all trees in the orchard must be removed and the orchard must remain closed for 3 years. Since 2020, if the number of trees infected with fire blight is less than 5% of the total trees in the orchard, only the infected tree and adjacent trees are removed in Korea. Three years after removal, the trees can be replanted after confirming that the orchard soil is free from E. amylovora. In this study, a protocol was established for detecting E. amylovora in soil via selective enrichment, using tryptic soy broth with 0.05% bile salts and 50 ㎍/ml cycloheximide, and real-time polymerase chain reaction. This protocol resulted in a 1,000-times improved detection limit for E. amylovora in soil samples compared to that in unenriched samples. Soil monitoring was performed for orchards where fire blight-infected trees had been removed 3-27 months prior; the selected orchards were monitored every 3 months. Monitoring confirmed that E. amylovora was not present in the soil at any site in any of the orchards. A new detection protocol facilitates the monitoring of E. amylovora in soil and could help permit the replanting of trees in orchards. Also monitoring results provide evidence that trees can be planted earlier.

Fabrication and characteristic evaluation of microfluidics chip integrated OLED for the light sources (OLED광원이 집적화된 마이크로 플루이딕칩의 제작 및 특성 평가)

  • Kim, Young-Hwan;Han, Jin-Woo;Kim, Jong-Yeon;Kim, Byoung-Yong;Seo, Dae-Shik
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.06a
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    • pp.377-377
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    • 2007
  • A simplified integration process including packaging is presented, which enables the realization of the portable fluorescence detection system. A fluorescence detection microchip system consisting of an integrated PIN photodiode, an organic light emitting diode (OLED) as the light source, an interference filter, and a microchannel was developed. The on-chip fluorescence detector fabricated by poly(dimethylsiloxane) (PDMS)-based packaging had thin-film structure. A silicon-based integrated PIN photo diode combined with an optical filter removed the background noise, which was produced by an excitation source, on the same substrate. The active area of the finger-type PIN photo diode was extended to obtain a higher detection sensitivity of fluorescence. The sensitivity and the limit of detection (LOD S/N = 3) of the system were $0.198\;nA/{\mu}M$ and $10\;{\mu}M$, respectively.

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A Study on Improved Detection Signature System in Hacking Response of One-Line Games (온라인 게임 해킹대응에서 Signature 기반 탐지방법 개선에 관한 연구)

  • Lee, Chang Seon;Yoo, Jinho
    • The Journal of Society for e-Business Studies
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    • v.21 no.1
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    • pp.105-118
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    • 2016
  • Game companies are frequently attacked by attackers while the companies are servicing their own games. This paper analyzes the limit of the Signature detection method, which is a way of detecting hacking modules in online games, and then this paper proposes the Scoring Signature detection scheme to make up for these problems derived from the limits. The Scoring Signature detection scheme enabled us to detect unknown hacking attacks, and this new scheme turned out to have more than twenty times of success than the existing signature detection methods. If we apply this Scoring Signature detection scheme and the existing detection methods at the same time, it seems to minimize the inconvenient situations to collect hacking modules. And also it is expected to greatly reduce the amount of using hacking modules in games which had not been detected yet.