• 한국수산과학회지
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• 제36권5호
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• pp.522-527
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• 2003

Feeding habits of Limanda yokohamae collected from the eelgrass bed in Kwangyang Bay were studied. L. yokohamae (1-16 cm SL) was a carnivore which consumed mainly polychaetes. Its diets included a significant quantity of amphipods (gammarids and caprellids) as well as small quantities of gastropods and ophiuroids. L. yokohamae showed ontogenetic changes in feeding habits. Small individuals less than 4 cm SL preyed mainly on amphipods. However, polychaetes were heavily selected with increasing fish size while the portion of the diet attributable to amphipods decreased sharply. Polychaetes were the major prey organisms for all seasons. Dietary breadth of each size class shows relatively low value, and this means that L. yokohamae depends on only few kinds of food organisms.

• 한국양식학회지
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• 제10권4호
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• pp.381-386
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• 1997

A series of experiments were conducted to compare the effects of various diluents in cold storage on the marbled sole, Limanda yokohamae sperm. Various diluents of glucose, L. yokohamae serum, marine fish Ringer's solution, sodium citrate and Ca2+ free artificial seawater (ASM) (tris-HCI, pH 7.4) containing 3 mM ethylene glycol-bis (2-aminoethyl ether) tetraacetic acid (EGTA) were used to store the sperm at 4℃. The storage effect was evaluated using motility index and survival rate of sperm. Glucose and sodium citrate were found to be better diluents which maintained high motility and survival rate of sperm for a storage period of 10 days. Some morphological changes of spermatozoa were observed during the cold storage with diluents. In particular, a detachment of the nuclear enveloped and of the plasma membrane from the nucleus in spermatozoa was observed. Morphological normality of the stored spermatozoa diluted with 0.3 M glucose was better than that of the stored spermatozoa undiluted or diluted with Ca2+ free ASW containing 3 mM EGTA.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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• pp.477-478
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• 2000

가자미과 (Pleuronectidae)에 속하는 문치가자미 (Limanda yokohamae)는 우리나라, 일본, 대만, 중국 등지에 분포하며 동일 속의 4종과 달리 우리나라 남.서해안에서만 서식하고 (김과 윤, 1994), 우리나라 서해안에서는 북위 37$^{\circ}$를 경계로 하계 및 동계 분포가 뚜렷하게 구분되는 계절회유성 어종으로 알려져 있다(김 등, 1994). (중략)

• Food Science and Biotechnology
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• 제14권4호
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• pp.551-553
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• 2005

Carbonic anhydrase III was found in liver of flounder, Limanda yokohamae. Protein was isolated from cytosolic extracts and identified using SDS-PAGE and isolectric focusing. Specific protein bands with molecular weight of 30 kDa and pIs of 7.0 and 6.5 were detected by Western blotting. This is the first report of identification of carbonic anhydrase III from L. yokohamae.

• Applied Microscopy
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• 제29권4호
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• pp.427-435
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• 1999

문치가자미의 정자형성과정을 미세구조적으로 관찰한 결과 체외수정을 하는 다른 경골어류의 정자형성과정과 유사한 것으로 나타났다. 정자변태과정 동안 염색질은 과립형태에서 구상으로 점차 응축하여 성숙 정자의 두부에서는 치밀하고 균질하게 된다. 정자는 두부와 미부로 구성되며, 첨체는 가지지 않는다. 두부 후방에서는 cytoplasmic collar가 관찰되었으며, cytoplasmic collar는 여덟개의 미토콘드리아를 가진다. 미부에서는 잘 발달된 axonemal lateral fin을 관찰할 수 있다. 미부 편모축사의 횡단면은 "9+2"의 미세소관 구조를 나타내며, 세포질내에 많은 수의 공포를 가진다.

• 한국어병학회지
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• 제14권1호
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• pp.54-58
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• 2001

The seasonal trends of plasma protein and glucose concentrations in marbled soles(Limanda yokohamae) and greenlings(Hexagrammos otakii), and the influence of transport stressor on those levels were investigated. Total plasma protein levels of marbled soles and greenlings in late spring and summer were significantly higher than those in winter(January). Plasma glucose levels were consistently increased according to elevation of water temperature both in marbled soles and greenlings. Transport stressor gave rise to decrease of plasma protein levels and increase of blood glucose levels.

• 한국양식학회지
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• 제12권4호
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• pp.275-281
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• 1999

The utility of RSV-LTR and carp beta-actin promoters was evaluated in a marine flatfish species, Limanda Yokohamae by examining successful expression of transgenic DNA in muscles (transfected by direct injection) and in early embryos (transformed by lipofected sperm). The expressed pattern of injected DNA in skeletal muscles was dependent on the DNA amount injected. The activity reached to maximal level at 48 hours post injection, and persisted up ot 1 month transiently. Gene transfer into early embryo of this species was successfully achieved using lipofected sperm with the efficiency ranging 36.8% to 48.1%. The expression of transgene during embryonic development was shown as stage-specific and transient.

• 환경생물
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• 제27권1호
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• pp.95-99
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• 2009

Transcriptomic changes in the brain of Limanda yokohamae were investigated to understand the environmental condition of Masan Bay, Korea. Differentially expressed genes (DEGs) in the brain of the flat fish from Masan Bay were identified by comparing those from the reference site Gangneung using annealing control primers-based polymerase chain reaction. The results demonstrated that two different kinds of the cytoplasmic ribosomal proteins, 40 s ribosomal protein S27a and ribosomal protein L6, were identified by the BLAST searching followed by sequence analysis. These findings suggest that environmental status of Masan Bay could hinder protein synthesis that is required for maintaining brain functions and thus cause the dysfunction of fish physiology.

• 한국어류학회지
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• 제11권1호
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• pp.46-51
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• 1999

가덕도 조간대 천해역에서 1998년 1월부터 12월까지 매월 갓후리그물(망목: 5 mm)로 채집한 문치가자미(Limanda yokohamae) 유어의 이석에 나타나는 미세구조와 일륜수를 관찰하여 연령 및 초기성장을 분석하였다. 문치가자미의 유어는 2월에서 4월까지 출현하였고 평균체장은 2월에 $22.6{\pm}1.77\;mm$ ($\pm$SD), 3월에는 $23.6{\pm}3.86\;mm$, 4월에는 $38.2{\pm}8.38\;mm$였다. 이석의 핵 직경은 $18{\sim}21\;{\mu}m$이었으며 부화정지선 이후부터는 좁은 동심원상의 성장선이 생성되고, 부화 후 48~56일이 지나면 이차성장핵이 형성되었다. 이 시기에 치어가 변태를 마치고 저서생황을 시작하여 천해역으로 이동하는 것으로 추정되었다. 이후부터는 성장속도가 빨라지고, 특히 전단부와 후단부의 성장이 빨라졌다. 일륜수로부터 역추산한 부화일은 11월 말에서 1월 초까지로 주로 12월에 부화되었다. 체장 (L)은 이석의 장반경 (R)에 대하여 L=0.055 R+5.81 ($r^2=0.88$) 로 회귀되었으며, 체장과 일륜수를 이용한 Gompertz 성장식은 $L_t=3.39e^{4.51(1-e^{-0.0067t})}$ ($r^2=0.81$) 로 추정되었다. 체장의 성장률은 부화 후 70 일 때는 0.35 mm/d에 서 120일 때는 0.55 mm/d로 자라면서 계속 성장률이 증가 추세를 보였다.

• 한국수산과학회지
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• 제39권5호
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• pp.391-397
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• 2006

This study correlated changes in estradiol-l7$\beta$ ($E_2$), testosterone (T), 17$\alpha$,20-dihydroxy-4-pregnen-3-one (DHP), and vitellogenin (VTG) levels with changes in the gonadosomatic index (GSI) and ovarian histology during the annual reproductive cycle of the wild marbled sole, Limanda yokohamae. Synchronous oocyte development occurs in this fish. Ovary maturity was classified into four periods, based on histological observations: the spawning (December to February), post-spawning (February to April), recovery (May to August), and vitellogenic (September to November) periods. Seasonal changes in the GSI were inversely correlated with water temperatures and reflected the degree of ovarian maturity. Plasma VTG levels were correlated with changes in the GSI, which increased from September to a peak in January, and levels remained comparatively high until February. Estradiol-17$\beta$ was at baseline levels (<0.11 ng/mL) during the spring and summer, and peaked rapidly (1.55$\pm$0.445 ng/mL) from October to January. Plasma T and DHP levels had a similar profile; they rose markedly during the spawning period and remained low (or were not detectable) from spring through autumn. These data indicate that changes in plsama steroid hormones and VTG levels are correlated with the annual ovarian activity of the marbled sole. Based on these results and published reports, it appears that in this species DHP is the most important maturation-inducing steroid and that T is also related to final maturation.