• Korean Journal of Medicinal Crop Science
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• v.19 no.1
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• pp.9-15
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• 2011

This study was conducted to establish the optimal condition for conservation of genetic resources and micropropagation of Lilium cernum. Induction of bulbet of L. cernum was highly effective (9.2 bulb/explant) on 1/2 SH (Schenk and Hildebrandt) medium supplemented with 1.0 mg/L TDZ (Thidiazuron) and 0.1 mg/L NAA (Naphthaleneacetic acid). The treatment of 0.1 mg/L NAA increased root development (6.4 root/explant) under the in vitro condition. In addition, treatments of AC (Activated Charcoal) and ventilation were enhanced to develop number of shoots and to elongate length of leaf, bulb and root. Futhermore, the process of short-term soil acclimatization was promoted to strengthen the plantlets induced under the in vitro condition.

• Journal of Plant Biotechnology
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• v.40 no.2
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• pp.65-71
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• 2013

Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, $3.0mg{\cdot}L^{-1}$ kinetin and 0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$ NAA or 2,4-D for callus induction. In media with $0.5{\sim}3.0mg{\cdot}L^{-1}$ kinetin and $0.1{\sim}1.0mg{\cdot}L^{-1}$ NAA and 2,4-D, 95~100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and $1.0mg{\cdot}L^{-1}$) in combination with BA (0, 1.0 and $2.0mg{\cdot}L^{-1}$) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with $50g{\cdot}L^{-1}$ sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Journal of Plant Biology
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• v.21 no.1_4
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• pp.29-32
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• 1978

The karyotype of Lilium cernum has been analysed by means of C-banding technique. Most of clones observed were 2n=24 chromosomes which consist of two pairs of submetacentric and ten pairs of subtelocentric chromosomes, among which two pairs of chromosomes(B and E) showed secondary constriction in the short arm. In addition to these chromosomes a small supernumerary telocentric chromosome was seen in the eight clones. Sixtyeight bands were observed in the twentytwo chromosomes of complement and one band in the supernumerary chromosome. A pair of chromosome (L) did not show any band. The bnads on the chromosome. A pair of chromosome (L) did not show any band. The bands on the chromosomes were distributed in the centromere, secondary constriction and intercalary regions of arms. Of the twelve pairs of chromosomes ten pairs showed symmetric banding patterns in each, but two pairs (I and K) showed asymmetric banding patterns.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.104-105
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.82-83
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• 2000

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.151-152
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• 2010

• Journal of Environmental Science International
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• v.12 no.2
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• pp.125-132
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• 2003

This study was executed by China along with the Tumen river, which formed border of North Korea and China. As a result 91 family, 282 genera, 363 species, 57 variety 4 species, total 424 taxa of vascular plants were identified. The examination area was include the region that had various vegetation such as upper swampy land, seashore dune, a river, a high mountain grassy plain, deciduous tree zone, evergreen coniferous forest zone. The followings are representative plants, which found in an examination area but not in South Korea. Picea koraiensis, Larix gmelini var. principis-ruprechtii, Betula playphylla, Ulmus pumila, Populus koreana, Ledum palustre var. diversipilosum, Rhododendron parvifolium, Salix brachypoda, Phacelurus latifolius, Carex gotoi, Carex capricornis, Calla palustris, Aquilegia flabellata var. pumila, Lilium davuricum, Astragalus membranaceus, Trifolium lupinaster, Ledebourieila seselodeds, Polemonium racemosum, Scutellaria baicalensis, Linnaea borealis, Thladiantha dubia, Inula helenium, Trollius hondoensis, Hypocaeris ciliata, Aconitum kirinense, Aconitum arcuatum, Potentilla viscosa var. macrophylla. And there are 21 rare and endangered species, which Korean Forest Sevice designated, such as Aconitum koreanum, Acorus calamus var. angustatus, Polygonatum stenophyllum, Drosera rotundifolia, Iris setosa, Jeffersonia dubia, Lilium davuricum, Lilium cernum, Paeonia obovata, Penthorum chinense, Phacelurus latifolius, Rosa marretii, Sparganium stoloniferum, Thymus quinquecostatus, Trillium tschonoskii, Utricularia japonica, Tripterospermum japonicum, Vaccinium uliginosum, Vaccinium vitis-idaea, etc. Especially, plants like, Acanthopanax senticosus, Jeffersonia dubia and Lillum cernum which have high values of ornamental, and medical use are in an extinction crisis. High swampy land was formed to upper stream of Tumen river. It showed representative vegitation, like Carex sp., Scirpus radirans, Eriophorum latifolium. Wetlands plant and a dune plant were mixed in down stream area of Tumen river. On a dune, seashore plants, such as Carex kobomugi, Salsola collina, Raphanus sativus var. raphanistroides, Rosa rugosa, Scutellaria strigillosa, Linaria japonica, Phacelurus latifolius were appeared frequently.

• Korean Journal of Environment and Ecology
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• v.18 no.2
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• pp.107-123
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• 2004

The vascular plants of Mt. Deog-yu area in the Baekdudaegan were recorded as 411 taxa; 91 families, 243 genera, 353 species, 53 varieties and 5 forma. From the floristic point of view, the Baekdudaegan, a main mountain range of the Korean peninsula, in the region of Mt. Deog-yu, belongs to the southern province in Korea, as the species like Cephalotaxus koreana, Lindera obtusiloba, Ilex macropoda and Stewartia koreana. are ranged in this region. But the species of the Northern element such as Betula costata, Betula ermani, Heloniopsis orientalis and Disporum ovale are also recorded in the region. Above the sea level from 1,000m, many sub alpine plant species are recorded. Rare and endangered plant species which are categorized by the Office of Forestry were 10 species such as Lilium cernum, Paeonia obovata, Abies koreana, Disporum ovale, Lilium distichum, Tricyrtis dilatata, Clematis chiisanensis, Bupleurum euphorbioides, Rhododendron schonoskii and Iris ensata var. spontanea. The protected plant species which are categorized by the Ministry of Environment were recorded only Lilium cernum. The Korean endemic plant species were recorded as 22 taxa; 12 families, 21 genera, 20 species 1 varieties, and 1 forma. Among them the plant species such as Cephalotaxus koreana, Abies koreana, Hosta capitata, Polygonatum lasianthum var. coreanum and Tricyrtis dilatata are representative ones in the region.

• Korean Journal of Environment and Ecology
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• v.19 no.2
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• pp.112-118
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• 2005

Based on the specific plant species for environmental assesment by the Ministry of Environment(MoE), a total of 68 taxa were identified; 29 taxa for the floristic degree(FD) I, 12 taxa for the FD II, 11 taxa for the FD III, 9 taxa for the FD IV and 6 taxa for the FD V. The endangered plant species, in Woraksan National Park, such as Lilium cernum and Berchemia berchemiaefolia are categorized as the Conservation Degree (CD) II which is designated by the MoE. The rare and endangered species such as Crypsinus hastatus, Lilium cernum, Berchemia berchemiaefolia, Lilium callossum, Gastrodia elata, Aristolochia contorta, Koelreuteria paniculata, Rhododendron tschonoskii, Scopolia japonica, Cypripedium macranthum, Paeonia japonica and Thymus quinquecostatus including 12 plant taxa are categorized as Rare and Endangered Plant Species by the Korea Forest Service.

• Korean Journal of Environment and Ecology
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• v.12 no.3
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• pp.191-211
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• 1998

The flora of the Kayasan National Park was surveyed from March to September, 1997. This area has very rich and diverse vascular plants; 613 taxa with 102 families, 323 genera, 522 species, 1 subspecies, 83 varieties, 5 forms and 2 hybrids. The rare and endangered plants species such as Lilium cernum Kom., Thymus quiquecostatus Celak., Cypripedium macranthum Sw., Tricyrtis dilatata Nakai, Abies koreana Wils., etc. were recorded. The plant species of 100 taxa such as Typha orientalis Presl, Corydalis maculata B. Oh et Y. Kim, Ajuga spectabilis Nakai, etc. were recorded for the first time in the area. The introduced plant species were found along the traffic road sides. The plant species of 14 taxa such as Lilium cernum Kom., Thymus quiquecostatus Celak., Abies koreana Wils., etc. which categorized as rare and endangered plants by the Office of Forestry were also recorded in the area(This species were mainly distributed in the habitats where easily threatened by human's activities).