• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.2
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• pp.117-125
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• 2023

In this study, the anti-inflammatory and anti-oxidative activities were compared for the extracts of non-fermented Ligustrum japonicum fruits (LJF) and fermented counterparts. U se of Latilactobacillus curvatus (L. curvatus) and Weissella minor (W. minor), isolated from the Jeju Chromis notata, as fermented strains led to the extracts of LJF-LC and LJF-WM in this experiment. The yield of each fermented extract (LJF-LC and LJF-WM) was 40.5 ~ 46.0%, higher than 29.5% of non-fermented extract (LJF). As a result of an activity experiment using RAW 264.7 macrophages stimulated by lipopolysaccaride (LPS), it was confirmed that LJF-WM, a fermented extract, has an excellent effect of inhibiting NO production in a concentration-dependent manner without cytotoxicity. Upon the screening of DPPH and ABTS⁺ radical scavenging activities, the fermented LJF-LC and LJF-WM showed comparable to the non-fermented LJF. In the study of cell protection effect using HaCaT keratinocytes damaged by hydrogen peroxide (H₂O₂), the fermented LJF-WM indicated protective effect against oxidative stress. In addition, quantitative analysis of a major constituent salidroside by HPLC indicated about 15.6 mg/g for the LJF-LC and 13.9 mg/g for the LJF-WM, which were higher than that of non-fermented LJF (12.0 mg/g). Based on these results, it was suggested that the fermented extract from L. japonicum fruits could be used as a natural cosmetics material with anti-inflammatory and anti-oxidative effects.

• 한국약용작물학회:학술대회논문집
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• 2007.11a
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• pp.321.1-321.1
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• 2007

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.2
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• pp.181-187
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• 2012

Ligustrum lucidum (L. lucidum) contains large quantities of ursolic acid and oleanolic acid. In this study, we evaluated anti-wrinkle effects of three parts of L. lucidum extracts. We found that L. lucidum extracts were not only innocuous to human skin fibroblasts but also significantly decreased the expression of both MMP-1 and MMP-2 and increased the expression of COL1A1. Among the three parts of L. lucidum extracts (i.e., fruit, cane, and leaf extracts), the fruit extract was found to contain the greatest amounts of ursolic acid and oleanolic acid. These three parts of L. lucidum extracts increased the expression of COL1A1 and decreased the expression of MMP-1 and MMP-2 in a dose-dependent manner. Especially, the fruit extract of L. lucidum had the greatest upregulating effect on COL1A1 and the greatest downregulating effect on MMP-1 and MMP-2 in a non-toxic and dose-dependent manner. These results suggest that L. lucidum, especially the fruit, could be used as active ingredients for functional cosmetics.

• Journal of Korean Society of Forest Science
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• v.85 no.4
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• pp.577-585
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• 1996

To seek effective methods for evaluating air pollution and acid rain injury, artificial acid mist(pH 2.5, 3.5 and 4.5) and ground water(pH 6.5) were treated on the potted seedlings of Ligustrum obtusifolium, Cercis chinensis, Hibiscus syriacus and Sophora japonica. Leaf chlorophyll contents, characteristics of leaf-injury, wettability-measurement of diameter of water-droplets on the leaf surface-among treatments were investigated. The results were summarized as follows. 1. Chlorophyll contents of Ligustrum obtusifolium and Hibiscus syriacus measured on June 3 were highest in pH 2.5 plot, but those of Cercis chinensis and Sophora japonica were relatively low level. Chlorophyll contents of Ligustrum obtusifolium measured on August 24 was highest in pH 2.5 plot, but those of Cercis chinensis, Hibiscus syriacus and Sophora japonica were highest in the control. 2. Changes of chlorophyll contents with acid mist treatments were differed among tree species. 3. For all the tested species, leaf injury(injured leaf number and rate, and injured leaf area) increased with decreasing pH levels of acid mist. 4. Leaf tissue injury seemed to be related with the wettability of the leaf surface. Measurement of diameter of water-droplets on the leaf surface might be useful criteria for acid rain or acid mist injury for the glabrous leaved species, such as, Cercis chinensis, Sophora japonica, etc.

• Journal of Ginseng Research
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• v.44 no.2
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• pp.291-299
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• 2020

Background: Ginseng (G) and Ligustrum lucidum Ait (LLA) are core traditional Chinese medicines in treating myelosuppression formula. The present study was designed to profile effect of G and LLA herb pair (G-LLA) on myelosuppressed mice. Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide (Cy). Hematopoietic function of bone marrow was measured by hemopoietic progenitor cell culture and peripheral blood count, and serum hemopoietic factors were tested by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. HPLC was used to measure 20 potential chemical components related to myelosuppression, including ginsenoside Rg₁, Re, Rb₁, Rc, Rb₂, Rb₃, Rd, Rk₃, Rh₄, 20 (S)-Rg₃, 20 (R)-Rg₃, Rk₁, Rg₅, salidroside, and so on. Results: G, LLA, and G-LLA improved the amount of peripheral blood cells and bone marrow cells of myelosuppressed mice (P < 0.01). They significantly increased the colony quantity of colony-forming unit-granulocyte macrophage, burst-forming unit-erythroid, colony-forming unit-erythroid, and colony-forming unit-megakaryocyte and amount of G₂/M and S phase cells (P < 0.01). They also significantly decreased the amount of hematopoiesis-related cytokines (P < 0.01). The content of chemical components in G-LLA changed, and the change of rare saponin was the most obvious. Conclusion: These results show that G-LLA herb pair might produce synergistic or complementary compatibility effects on bone marrow suppression after chemotherapy. It suggests that the substance basis of G-LLA for treating bone marrow suppression may be effective chemical components.

• The Korea Journal of Herbology
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• v.32 no.2
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• pp.25-32
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• 2017

Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, anti-hypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an anti-inflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages. Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) were determined by Western blot analysis. Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$ in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 $NF-{\kappa}B$ in RAW 264.7 macrophages involving suppression of $NF-{\kappa}B$ activation. Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of $NF-{\kappa}B$ activation in LPS-treated RAW 264.7 macrophages.

• Journal of Korean Ophthalmic Optics Society
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• v.10 no.4
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• pp.365-373
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• 2005

Antioxidative activities of methanol extracts of 7 natural products which have been orientally used or studied as ophthalmic drugs were investigated. All of 7 extracts assayed - Rubus coreanus, Dendrobium monile, Eriocaulon sieboldianum, Ligustrum japonicum, Cassia tora, Cicadae Periostacum and Polygonatum odoratum var pluriflorum had some electron-donating ability to OPPH radicals, in which R. coreanus and D. monile showed strong activity as similar as ascorbic acid or BHT. At the concentration of $200{\mu}g/m{\ell}$, methanol extract of L. japonicum showed superoxide dismutase-like activity the same as 0.1 mM ascorbic acid and methanol extract of R. coreanus inhibited effectively the production of ABTS radical produced by $H_2O_2$. Furthermore, methanol extract of C. tora showed the highest nitrite scavenging ability. The inherent antioxidative activities of these natural products appear to be responsible for its ophthalmic effects.

• Journal of Korean Society for Atmospheric Environment
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• v.7 no.3
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• pp.208-218
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• 1991

To compare the resistance of landscape trees by acid rain, simulated acid rain was treated on the seeds of 6 coniferous species (Pinus densiflora, Pinus thunbergii, Pinus koraiensis, Taxus cuspidata, Cedrus deodara, Thuja occidentalis) and 6 broad-leafed species (Ligustrum obtusifolium, Acer Buergerianum, Acer Ginnala, Styrax obassia, Cornus Kousa, Magnolia obovata). The experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agri-Tech Junior College. The seeds of 12 tree species were planted on the nursery soil in the early spring of 1991. The regime of artificial acid rain, in terms of spray frequency per monthly and spray amount at year records. Simulated acid rain (pH 2.0, pH 4.0, pH 5.6 as control) containing sulfuric and nitric acid in the ratio of 3:2 (chemical equivalant basis) diluted ground water, were treated on the experimental plants under condition of cutting off the natural precipitation with vinyl tunnel, during the growing season (April 8 to August 31) in 1991. Seedling establishment, seedling growth and nutrient contents in needles and leaves were measured and compared among the treatment. The results were summerized as follows; 1. In general, coniferous species were more resistant than broad-leafed species in the resistant comparisons of landscape trees by simulated acid rain. But there were resistant tree species to acid rain among the broad-leafed species. 2. Among 6 coniferous species used in this experiment, Pinus densiflora, Pinus koraiensis, Taxus cuspidata, Cedrus deodara and Pinus thunbergii revealed the resistant tree species to acid rain. But Thuja occidentalis was very susceptible to acid rain, because of no germination in pH 2.0 treatment plot of acid rain. 3. Among 6 broad-leafed species used in this study, Magnolia obovata, Styrax obassia and Ligustrum obtusifolium showed relative resistant tree species to acid rain. But Acer Buergerianum, Acer Ginnala and Cornus Kousa were very susceptible to acid rain, because of no germination in pH 2.0 plot of acid rain treatment.

• Asian Journal of Turfgrass Science
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• v.12 no.3
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• pp.195-214
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• 1998

The leaf persisting and seasonal leaf color characteristics of deciduous woody landscape plants were investigated through the field survey in Suwon region. The leaf period was about 270 days from March 20, 1992 when Prunus padus was leaf spreading to December 17, 1992 when Lonicera sempervirens was leaf falling. The plants of leaf period over 240 days were Elaegagnus umbellata var. coreana, Salix pseudo-lasiogyn, Ligustrum obtusi-folium, Rosa multiflora, Lonicera sempervirens and Clematis viticella. The woody landscape plants bearing the red leaf by KBS standard color number before fall foliage coloring were Prunus salicina, Berberis thunbergii 'Atropurpurea', Acer platanoides 'Red King', Acer palmatum var. Pendula 'Crimson Queen', Parthenocissus tricuspidata, Nyssa sylvatica, Acer palmatum var. sanguineum, and the yellow leaf, Berberis thunbergii Variegata 'Aurea', Physocarpus opulifolius 'Luteus', and Forsythia koreana 'Seoul Cold', Ligustrum X vicaryi which is variegation. The fall foliage color period was about 100 days from September 6, 1992 when Euonymus alatus was fall foliage coloring, to December 17, 1992 when Lonicera sempervirens was leaf falling. (Table 24) And in terms of color based on KBS standard color number, 60.7% of fall foliage were yellow, 37.4%, red, respectively.

• Journal of Haehwa Medicine
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• v.25 no.1
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• pp.133-144
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• 2016

Objectives : This study was aimed to investigate the effect of ACLL extract (Artemisia capillaris Thunb, Curcuma longa L, Lycium chinense, Ligustrum lucidum Aiton complex extract) in a dyslipidemia animal model induced by a high-fat diet. Methods : ACLL extracts was administered at 200 mg/kg, and 400 mg/kg to Apoe mice, where arthritis was induced by high cholesterol diet. After mice were treated with GO for 4 weeks, we measured AST, ALT, BUN and lipid levels (total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride), atherogenic index(AI) and cardiac risk factor(CRF). Results : Serum AST, ALT, creatinine, BUN levels were not changed by ACLL extract do not show any toxic effects. Also, ACLL extract groups were found to have atherogenic Index and cardiac risk factor as well as lipid metabolism improvement (total cholesterol, LDL cholesterol and triglyceride decrease). Conclusions : We suggest that ACLL extract may have the control effects of Dyslipidemia by improving lipid metabolism.