• Title/Summary/Keyword: Liguid Nitrogen

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Kluyveromyces marxianus var. marxianus IFO 1735에 의한 Inulin Fructotransferase의 생산 및 이용에 관한 연구

  • 김재근;판정척부
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.277-285
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    • 1997
  • Kluyveromyces marxianus var. marxianus isolated as an inulin-assimilating microorganism produces inulin fructotransferase (inulaseII) which catalyses the conversion of inulin into di-D-fructofuranose 1, 2' : 2, 3' dianhydrde (DFAIII). The DFA produced by the organism was isolated by using active carbon column, and identified as DFAIII by high performance liguid chromatography. The culture medium giving maximum inulaseII production was found to consist of 1% sucrose and 0.75% yeast nitrogen base (YNB). The inulasell production was induced by inulin or sucrose as a carbon source and increased by addition of YNB as a nitrogen source. Optimal initial pH of the culture medium, culture temperature and medium volume for the enzyme production were pH 4.7, 30$\circ$C and 140 ml, respectively. Under the optimal conditions described above, the enzyme activity in the culture supematant reached 4.2 units/ml after cultivation for 36 h. The DFAIII was accumulated at 13.25 mg/ml after 48 h of culture in the Jerusalem artichoke tuber medium.

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Cryosurgical Treatment of Ameloblastoma: Case Report (법랑아세포종의 냉동수술 치험례)

  • Choi, Moon-Gi
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.34 no.3
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    • pp.226-232
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    • 2012
  • Although an ameloblastoma is a benign tumor histologically, it may act malignantly. It has locally destructive and recurrent tendencies. Many different strategies have been attempted in order to cure an ameloblastoma including curettage, enucleation, marsupialization, and resection with a safty margin. Curettage, enucleation, and marrsupialization can be classified into a conservative treatment and resection with a safty margin can be classified into a radical treatment. Radical treatment has better results than the conservative treatment. Thus, more radically conservative treatment methods are needed in order to improve the treatment results. The cryosurgery can be applied an ameloblastoam. In particular, with regards to the solid and intramural type, the application of the cryosurgery has its advantages over the conservative treatment. After resection of the diseased area we don't need to discard the diseased segment. Instead, by placing the segment in liguid nitrogen, the diseased segment can use the autogenous tray for packing several bone materials.

Insulation Design and Test of Model Windings for the Development of High Temperature Superconducting Transformer (고온초전도변압기 개발을 위한 모델 권선의 절연 설계 및 평가)

  • Joung, Jong-Man;Baek, Sung-Myeong;Kwak, Dong-Sun;Kim, Sang-Hyun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.08a
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    • pp.19-22
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    • 2003
  • In the response to increasing the demands for electrical energy, much effort aimed to develop and commercialize 1MVA HTS power equipments that is supported by a grant from center for Applied Superconductivity Technology of the 21st Century Frontier R&D Program funded by the Ministry of Science and Technology is going on in Korea. For the development, the cryogenic insulation and winding insulation of it in this paper are discussed. In the first many types of dielectric insulating tests were carried out. In detail Breakdown characteristics of $LN_2$, FRP and turn insulating films, flashover characteristics along the FRP surface in $LN_2$ were verified after distinguishing insulation components in HIS windings. And then model windings were designed and insulation test was conducted. These included a AC withstand voltage test of 50kV rms and a lightning impulse test of 150kV at peak.

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