• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2000.05a
• /
• pp.446-446
• /
• 2000

Specific polyclonal and/or monoclonal antibodies (MAbs) to immunoglobulins (Igs) and their subunits have proved to be valuable tools in immunological research and in immunological assays. In this study, we developed and characterized MAbs against flounder serum Igs. To obtain the pure flounder serum Igs, mouse IgG (mIgG) was immunized to flounder. Flounder Igs were purified by using mIgG-agarose affinity column chromatography. The structure of purified flounder Ig was observed, on denatured SDS-PAGE, to be composed of two heavy chains (77 and 72 kd) and two light chains (28 and 26 kd). MAbs were produced by fusion of myeloma cells (SP2/0) with Balb/c mouse spleen cells previously primed with the flounder Igs. Finally, three hybridoma clones, FIM 511, FIM 519 and FIM 562 were established to recognize both 2 heavy chains, 26 kd of light chain and 28 kd of light chain, respectively. On the other hand, the flounder immune sera collected on the weekly basis were tested on ELISA and immunoblot analysis whether boosting effect is present in flounder humoral immune system. As a result, the secondary immune response in flounder was ascertained on ELISA, but not on immunoblot analysis. Further, we observed an alteration of serum protein levels following immunization. Our MAbs and basic information on flounder humoral immune system obtained in this study will be helpful to control and monitor the efficiency of fish vaccines and therapeutic process of flounder diseases.

• Carbon letters
• /
• v.13 no.1
• /
• pp.34-38
• /
• 2012

Successful application of graphene requires development of various tools for its chemical modification. In this paper, we present a Raman spectroscopic investigation of the effects of UV light on single layer graphene with and without the presence of $O_2$ molecules. The UV emission from a low pressure Hg lamp photolyzes $O_2$ molecules into O atoms, which are known to form epoxy on the basal plane of graphene. The resulting surface epoxy groups were identified by the disorder-related Raman D band. It was also found that adhesive residues present in the graphene samples prepared by micro-mechanical exfoliation using adhesive tape severely interfere with the O atom reaction with graphene. The UV-induced reaction was also successfully applied to chemical vapor deposition-grown graphene. Since the current method can be readily carried out in ambient air only with UV light, it will be useful in modifying the surfaces of graphene and related materials.

• Korean Journal of Optics and Photonics
• /
• v.24 no.6
• /
• pp.338-341
• /
• 2013

Prothrombin Time (PT) is used as a measure of blood coagulation time. An optical analysis of the signal generating mechanism is described here for the reflection-type arrangement. Thromboplastin added into serum or whole blood initiates the coagulation process. We added a sufficient amount of magnetic particles into the sample before adding thromboplastin and subjected the mixture to a rotating magnetic field. The laser light gets reflected in a periodically modulated fashion with respect to the magnetic field rotation. Analysis of this decaying modulation could produce a reasonable coagulation time. We also introduce a simulation model to explain the signal generating mechanism using LightTools.$^{TM}$

• Korean Journal of Optics and Photonics
• /
• v.18 no.4
• /
• pp.264-269
• /
• 2007

Today's research has been focused on changing the light source from filament to LED for luminarie illumination to overcome the shortcoming of a filament. The purpose of this research is to make an appropriate high power LED package structure for luminarie. We simulated radiation patterns of the various structures by the ray tracing simulator (Light Tools), and also analyzed the radiation patterns using an LED test system (OL770). As we increased an inner reflector angle, the radiation pattern split into two peaks and the angle between two peaks became larger. In addition, when we increased an outer reflector angle, the angle between side peaks gradually decreased, while it increased again when the angle reach $50^{\circ}$. These results could be understood from the ray tracing of the light reflected from two reflectors. We made the high power LED package for luminarie on the condition of the optimized structure which was made by ray tracing simulation results, and we measured the radiation patterns by using an LED test system, and these results were well matched to the simulation results.

• International Journal of Internet, Broadcasting and Communication
• /
• v.12 no.4
• /
• pp.128-136
• /
• 2020

In this paper, we presented a simple hologram recording set for learners to broaden their program choices and experience holographic techniques with lifelong education programs. In order to obtain quality 3D images, the quality of light sources and recording mediums, which are the main elements of hologram recording, must be good. In addition, due to the characteristics of the hologram, the ambient vibrations shall be minimized for accurate interference pattern recording. The simple hologram recording set presented in this paper has not significantly restricted in space and location, and anyone can easily experience it at a reasonable price compared to the existing hologram production method. Also, it can make two different type of hologram (transmission and reflection) for providing easy access to recording and reconstruction of the hologram. Experiments have shown that holographic manufacturing practices are a very useful way to educate the public on optics and photonics technologies.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.23 no.1
• /
• pp.24-28
• /
• 2013

In this paper, a procedure of design and development of a child-sized humanoid robot is described. The design concept for a humanoid robot was proposed and the mechanism of the humanoid robot which is more than 1 meter tall was designed by using 3D design tools. By considering the lightweight of the robot, the hardware for the robot was designed for optimal performance. The frames and links of the robot designed by 3D design tools was manufactured through precision machining with the material which is light and have a good strength. The manufactured child-sized humanoid robot was experimented with basic motions applied inverse kinematics and balance control, and the performance of the motions were verified.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.17 no.2
• /
• pp.351-358
• /
• 1993

Recently a high speed spindle system for machine tools has attracted considerable attention to reduce the machining time, to improve the machining accuracy, to perform the machining of light metals and hard materials and to unite the cutting and grinding processes. In this study, a high speed spindle system is developed by applying the oil-air lubrication method, angular contact ball bearings, injection nozzles with dual orifices and so on. And a lubrication experiment for evaluating the performance of the spindle system is carried out. Especially, in order to establish the lubrication conditions related to the development of a high speed spindle system, the effects of oil supply rate, rotational spindle speed and so on are studied and discussed on the bearing temperature rise, bearing temperature distribution and frictional torque. And the effect of spindle system structure on the bearing temperature distribution is investigated.

• Journal of the Korean Society for Precision Engineering
• /
• v.31 no.3
• /
• pp.189-197
• /
• 2014

Diffractive optical elements (DOEs) - in general a complex pattern of micro- and nano-scale structures - can modulate and transform light in a predetermined way. Their importance is being increased nowadays because they can be designed to handle a number of simultaneous tasks. In view point of machining DOEs, it is a big challenge to fabricate micro- and nano-scale structures on a free-form surfaces. In this paper, the state-of-the-art of the ultra-precision machine tools is reviewed. Also some technical issues which determine the machine tool accuracy are discussed.

• Food Science and Industry
• /
• v.50 no.1
• /
• pp.2-10
• /
• 2017

Human have eaten various traditional fermented foods for a numbers of million years for health benefit as well as survival. The beneficial effects of fermented foods have been resulted from complex microbial communications within the fermented foods. Therefore, the holistic approaches for individual identification and complete microbial profiling involved in their communications have been of interest to food microbiology fields. Microbiome is the ecological community of microorganisms that literally share our environments including foods as well as human body. However, due to the limitation of culture-dependent methods such as simple isolations of just culturable microorganisms, the culture-independent methods have been consistently developed, resulting in new light on the diverse non-culturable and hitherto unknown microorganisms, and even microbial communities in the fermented foods. For the culture-independent approaches, the food microbiome has been deciphered by employing various molecular analysis tools such as fluorescence in situ hybridization, quantitative PCR, and denaturing gradient gel-electrophoresis. More recently, next-generation-sequencing (NGS) platform-based microbiome analysis has been of interest, because NGS is a powerful analytical tool capable of resolving the microbiome in respect to community structures, dynamics, and activities. In this overview, the development status of analysis tools for the fermented food microbiome is covered and research trend for NGS-based food microbiome analysis is also discussed.

• Journal of the Korean Society for Precision Engineering
• /
• v.11 no.1
• /
• pp.123-128
• /
• 1994

A high speed spindle system for machine tools can be used to reduce the machining time, to improve the machining accuracy, to perform the machining of light metals and hard materials, and to unite the cutting and grinding processes. In this study, a high speed spindle system is developed by applying the oil-air lubrication method, angular contact ball bearings, injection nozzles with dual orifices, cooling jacket and so on. And an air cooling experiment for evaluating the performance of the spindle system is carried out. Especially, in ofder to establish the air cooling conditions related to the development of a high speed spindle system, the effects of cooling air pressure, oil supply rate, air supply rate and rotational spindle speed are studied and discussed on the bearing temperature rise and frictional torque. Also the effects of cooling air pressure, rotational spindle speed and spindle system structure is investigated on the bearing temperature distribution. The experiment on the test model reveals the usefulness of the air cooling method.