• Title/Summary/Keyword: Light dependent

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대 . 소맥에 있어서 저온 및 수분부족에 의한 유리 Prolie의 축적 (THE ACCUMULATION OF FREE PROLINE AT LOW TEMPERA TURES AND WATER STRESS IN BARLEY AND WHEAT)

  • 최원열;아스피날돈
    • 한국작물학회지
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    • 제24권1호
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    • pp.57-65
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    • 1979
  • 대.소맥에 대한 유리 Proline 축적의 온도범위, 장기저온처리의 경향, 저온과 수분부족에 있어서 광의재생의 여부 및 수종의 식물에 대한 종간 및 종내의 변이 등 일련의 반응생리와 축적기작에 대한 실험결과를 요약하면 다음과 같다. 1. 대맥은 8$^{\circ}C$나 그 이하의 온도에서 축적되나 소맥은 12$^{\circ}C$나 그 이하의 온도에서 Proline을 축적하였다. 2. 대.소맥에 있어서 신장억제온도와 Proline을 축적하기 시작하는 온도는 거의 일치하였다. 3. 엽절편을 5$^{\circ}C$의 저온에 둔 것은 29일 생존하였으며 그동안 Proline을 축적하였으나 2$0^{\circ}C$에 둔 것은 13일 밖에 생존하지 못하였으며 그 동안 축적은 없었다. 4. 완전식물체엽과 엽절편 공히 5$^{\circ}C$의 저온에 서의 축적은 광의재생이였다. 5. 광의재생은 Proline의 생합성을 위한 전구물질의 급여로는 대체될 수 없었다. 6. 수분부족에 의한 Proline 축적은 2$0^{\circ}C$에서는 광의재생이 아니였으나 5$^{\circ}C$에서는 광의재생이였다. 7. 저온과 수분부족에 대한 종간 및 종내의 축적변이는 높은 편이였다. 그러나 수분부족이 저온에 대한 변이보다 높았다. 8. 저온과 수분부족에 의한 Proline 축적의 기작은 상당히 상이한 것 같다.

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인삼 틸라코이드에서 광계II의 LHCP 인산화와 형광 Quenching (LHCP phosphorylation and Chlorophyll-Fluorescence Quenching of PSII in Ginseng Thylakoid Members)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • 제16권2호
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    • pp.124-128
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    • 1992
  • 인삼의 엽소현상과 광계 II(photosystem II)의 광수확 엽록소-단백질 복합체(LHCP II)의 광에너지 분배 기작과의 관계를 구명코저 LHCP II의 인산화에 따른 형광 quenching과 광량별 인산화 정도, 그리고 단백질 조성을 조사하였다. 인삼은 DCMU의 존재 하에서 photosystem II의 형광발생량이 양지식물인 콩에 비해 많았으며, 인간화에 따른 형광 quenching율도 현저히 높았다. 또한, 강광(25k1ux 이상)에서 인삼은 인산화에 따른 형광 quenching율이 콩에 비해서 2배정도 높다는 사실을 확인하였다. 엽록소-단백질 복합체(CP-complex)의 조성비율 및 LHCP II를 구성하고 있는 단백질의 앙과 수적인 면에서 비교식물과 큰 차이를 나타내었는데, 24~29kD 범위에서 인삼은 25, 26, 27kD의 major 밴드와 24, 25.3, 28.3kD의 minor밴드로 구성되어 있었으며 광량에 의존적으로 인산화가 증가하는 인삼의 LHCP II 단백질은 24kD 이었다.

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Ardipusilloside-I stimulates gastrointestinal motility and phosphorylation of smooth muscle myosin by myosin light chain kinase

  • Xu, Zhili;Liang, Hanye;Zhang, Mingbo;Tao, Xiaojun;Dou, Deqiang;Hu, Liping;Kang, Tingguo
    • The Korean Journal of Physiology and Pharmacology
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    • 제21권6호
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    • pp.609-616
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    • 2017
  • Ardipusilloside-I is a natural triterpenoid saponin, which was isolated from Ardisia pusilla A. DC. The aim of the study was to evaluate the stimulation of ardipusilloside-I on gastrointestinal motility in vitro and in vivo. The experiment of smooth muscle contraction directly monitored the contractions of the isolated jejunal segment (IJS) in different contractile states, and the effects of ardipusilloside-I on myosin were measured in the presence of $Ca^{2+}$-calmodulin using the activities of 20 kDa myosin light chain ($MLC_{20}$) phosphorylation and myosin $Mg^{2+}$-ATPase. The effects of ardipusilloside-I on gastro emptying and intestinal transit in constipation-predominant rats were observed, and the MLCK expression in jejuna of constipated rats was determined by western blot. The results showed that, ardipusilloside-I increased the contractility of IJS in a dose-dependent manner and reversed the low contractile state (LCS) of IJS induced by low $Ca^{2+}$, adrenaline, and atropine respectively. There were synergistic effects on contractivity of IJS between ardipusilloside-I and ACh, high $Ca^{2+}$, and histamine, respectively. Ardipusilloside-I could stimulate the phosphorylation of $MLC_{20}$ and $Mg^{2+}$-ATPase activities of $Ca^{2+}$- dependent phosphorylated myosin. Ardipusilloside-I also stimulated the gastric emptying and intestinal transit in normal and constipated rats in vivo, respectively, and increased the MLCK expression in the jejuna of constipation-predominant rats. Briefly, the findings demonstrated that ardipusilloside-I could effectively excite gastrointestinal motility in vitro and in vivo.

전극 변화에 따른 유기 발광 소자의 내장 전압 (Built-in voltage depending on electrode in organic light-emitting diodes)

  • 윤희명;이은혜;이원재;정동회;오용철;김태완
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 춘계학술대회 논문집
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    • pp.14-16
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    • 2008
  • Built-in voltage in organic light-emitting diodes was studied using modulated photocurrent technique ambient conditions. From the bias voltage-dependent photocurrent, built-in voltage of the device is determined. The applied bias voltage when the magnitude of modulated photocurrent is zero corresponds to a built-in voltage. Built-in voltage in the device is generated due to a difference of work function of the anode and cathode. A device was made with a structure of anode/$Alq_3$/cathode to study a built-in voltage. ITO was used as an anode, and Al and LiAl were used as a cathode. A layer thickness of Al and LiAl were 100nm. Obtained built-in voltage is about 1.0V in the Al layer was used as a cathode. The obatined built-in voltage is about 1.6V in the LiAl layer was used as a cathode. The result of built-in voltage is dependent of cathode. We can see that the built-in voltage increase up to 0.4V when the LiAl layer was used as the cathode. These results correspond to the work function of LiAl which is lower than that of Al. As a result, the barrier height for an electron injection from the cathode to the organic layer could be lowered when the LiAl was used as a cathode.

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Aloe-Emodin-Mediated Photodynamic Therapy Attenuates Sepsis-Associated Toxins in Selected Gram-Positive Bacteria In Vitro

  • Otieno, Woodvine;Liu, Chengcheng;Ji, Yanhong
    • Journal of Microbiology and Biotechnology
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    • 제31권9호
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    • pp.1200-1209
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    • 2021
  • Sepsis is an acute inflammatory response that leads to life-threatening complications if not quickly and adequately treated. Cytolysin, hemolysin, and pneumolysin are toxins produced by gram-positive bacteria and are responsible for resistance to antimicrobial drugs, cause virulence and lead to sepsis. This work assessed the effects of aloe-emodin (AE) and photodynamic therapy (PDT) on sepsis-associated gram-positive bacterial toxins. Standard and antibiotic-resistant Enterococcus faecalis, Staphylococcus aureus, and Streptococcus pneumonia bacterial strains were cultured in the dark with varying AE concentrations and later irradiated with 72 J/cm-2 light. Colony and biofilm formation was determined. CCK-8, Griess reagent reaction, and ELISA assays were done on bacteria-infected RAW264.7 cells to determine the cell viability, NO, and IL-1β and IL-6 pro-inflammatory cytokines responses, respectively. Hemolysis and western blot assays were done to determine the effect of treatment on hemolysis activity and sepsis-associated toxins expressions. AE-mediated PDT reduced bacterial survival in a dose-dependent manner with 32 ㎍/ml of AE almost eliminating their survival. Cell proliferation, NO, IL-1β, and IL-6 cytokines production were also significantly downregulated. Further, the hemolytic activities and expressions of cytolysin, hemolysin, and pneumolysin were significantly reduced following AE-mediated PDT. In conclusion, combined use of AE and light (435 ± 10 nm) inactivates MRSA, S. aureus (ATCC 29213), S. pneumoniae (ATCC 49619), MDR-S. pneumoniae, E. faecalis (ATCC 29212), and VRE (ATCC 51299) in an AE-dose dependent manner. AE and light are also effective in reducing biofilm formations, suppressing pro-inflammatory cytokines, hemolytic activities, and inhibiting the expressions of toxins that cause sepsis.

Photo-sintering of Silaver Nanoparticles using UV-LED

  • Lee, Jaehyeong;Kim, Minha;Kim, Donguk
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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    • pp.88.1-88.1
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    • 2015
  • In recent printed electronics technology, Photo-Sintering, a technique for sintering materials using a light source, has attracted attention as an alternative to time-consuming high-temperature thermal processes. The key principle of this technique is the selective heating of a strongly absorbent thin film, while preventing the heating of the transparent substrate by the light source. Many recent studies have used a flash lamp as the light source, and investigated the material-dependent effect of the width or intensity of the pulsed light. However, the flash lamp for sintering is not suitable for industry yet, because of needing too high power to sinter for a large scale. In energy-saving and large-scale sintering, LED technologies would be very useful in the near future. In this work, we investigated a sintering process for silver nanoparticles using UV-LED array. Silver nanoparticles in ink were inkjet-printed on a $1{\times}1cm$ area of a PET film and photo-sintered by 365 nm UV-LED module. A sheet resistance value as low as $72.6m{\Omega}/sq$ (2.3 - 4.5 times that of bulk silver) was obtained from the UV-LED sintering at 300 mW/cm2 for 50 min.

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PCM 혼입 경량기포콘크리트 패널 개발을 위한 기초적 연구 ((An) experimental study on the development of lightweight concrete using the PCM)

  • 임명관;오돈투야;김영호;최동욱
    • KIEAE Journal
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    • 제14권4호
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    • pp.133-138
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    • 2014
  • The present study was carried out to assess the basic material properties and thermal behavior of light-weight foamed concrete panel mixed with PCM (Phase Changing Material). To do so, this study fabricated light-weight foamed concrete (1.0kg/m3) in pre-foaming method and mixed it with PCM micro capsule of 1-dodecanol and melamine to examine its physical and thermal properties. The results confirmed strength reinforcement effect by proper replacement ratio of fly-ash, which is an industrial by-product, and PCM. In addition, it found out that PCM-mixed light-weight foamed concrete had time delay and temperature reduction effect within the range of PCM phase transition according to the rise of outdoor temperature. It was also observed that the insulation performance of PCM-mixed light-weight foamed concrete was more dependent upon thickness than PCM replacement ratio.

A Chemometric Aided UV/Vis Spectroscopic Method for Kinetic Study of Additive Adsorption in Cellulose Fibers

  • Chal, Xin-Sheng;Zhou, Jinghong;Zhu, Hongxiang;Huang, Xiannan
    • 한국펄프종이공학회:학술대회논문집
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    • 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.1
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    • pp.137-140
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    • 2006
  • This paper describes a technique combining chemometrics with UV spectroscopy for the determination of the concentra tions of two tissue additives (i.e., wet strength and softening agents) in a cellulose fiber containing solution. In single as ent solutions, the concentration of the additive can be measured by UV spectroscopy at the wavelength where the species having absorption. For a binary (i.e., containing two additives) solution system, the spectral characterization is very complicated. However, if aided by a chemometrical calibration technique, each additive in the binary solution can be quantified simultaneously. The present method is very rapid and simple, it can easily perform a continuous measurement in the changes in the additives' concentration after fiber addition, and therefore this becomes a valuable tool for the adsorption kinetics study of chemical additives onto the cellulose fibers. The time-dependent adsorption behaviors of the wet-strength, softening agent, and their both on fiber were also presented.

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Light intensity controlled wrinkling patterns in photo-thermal sensitive hydrogels

  • Toh, William;Ding, Zhiwei;Ng, Teng Yong;Liu, Zishun
    • Coupled systems mechanics
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    • 제5권4호
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    • pp.315-327
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    • 2016
  • Undergoing large volumetric changes upon incremental environmental stimulation, hydrogels are interesting materials which hold immense potentials for utilization in a wide array of applications in diverse industries. Owing to the large magnitudes of deformation it undergoes, swelling induced instability is a commonly observed sight in all types of gels. In this work, we investigate the instability of photo-thermal sensitive hydrogels, produced by impregnating light absorbing nano-particles into the polymer network of a temperature sensitive hydrogel, such as PNIPAM. Earlier works have shown that by using lights of different intensities, these hydrogels follow different swelling trends. We investigate the possibility of utilizing this fact for remote switching applications. The analysis is built on a thermodynamic framework of inhomogeneous large deformation of hydrogels and implemented via commercial finite element software, ABAQUS. Various examples of swelling induced instabilities, and its corresponding dependence on light intensity, will be investigated. We show that the instabilities that arise have their morphologies dependent on the light intensity.

벤젠제거에 대한 광촉매 효율의 여기광원 의존성 (Excitation Light Source Dependence of Photo-catalytic Efficiency for Benzene Removal)

  • 최용석;김성진;한영헌;유순재;이은아;김학수;김송강
    • 한국전기전자재료학회논문지
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    • 제18권6호
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    • pp.510-514
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    • 2005
  • We have investigated the excitation-light source dependence of photo-catalytic efficiency for the benzene removal. The photo-catalytic module for the benzene removal is fabricated by a combination of GaN-based ultraviolet light-emitting diode (UV GaN-LED) and $TiO_2$ thin film coated on an aluminum plate. The benzene reduction rates of 365 nm and 375 nm modules at 60 mA junction current are approximately $8.95\;\%/Hr$ and $9.2\;\%/Hr$, respectively, which indicates that 365 nm GaN-LED is more effective than 375 nm GaN-LED. The benzene reduction efficiency is also noticeably dependent on the excitation wavelength and excitation-light power, as well as it is increased with the shorter wavelength and higher excitation power. This result exhibits that UV GaN-LED is useful to remove the volatile organic compounds (VOCs) existing in the environment.