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Egg Development and Early Life History of Korean Spined Loach, $Iksookimia$ $koreensis$ (Pisces: Cobitidae) (참종개 $Iksookimia$ $koreensis$의 난발생 및 초기생활사)

  • Ko, Myeong-Hun;Park, Sang-Yong;Bang, In-Chul
    • Korean Journal of Ecology and Environment
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    • v.45 no.1
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    • pp.93-101
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    • 2012
  • In the present study, egg development and early life history of Korean spined loach, $Iksookimia$ $koreensis$, were observed. Adult fish were sampled using spoon nets in Okgye-ri, Hoengseong-gun, Gangwon-do, Korea in July 2010. Eggs were obtained after injecting females with Ovarprim. Eggs were then artificially fertilized in the laboratory using the dry method. Mature eggs were slightly adhesive and transparent with a light yellowish color, and measured $1.40{\pm}0.04mm$ (mean${\pm}$SD) in diameter. Hatching of the embryo occurred approximately 50 h after fertilization in the water at $23^{\circ}C$, and newly hatched larvae were averaged $4.7{\pm}0.21mm$ in total length. 5 days after hatching, the averaged total length of larvae was $7.1{\pm}0.25mm$ and their yolk sacs had been completely absorbed. 17 days after hatching, fish started to enter the juvenile stage and reached $12.2{\pm}1.10mm$ in total length. 80 days after hatching, the band patterns and external form of juvenile fish were similar to those of adults, and they averaged $31.0{\pm}3.98mm$ in total length.

Ultrastructural Description on Oogenesis of the Melania Snail, Semisulcospira libertina libertina (Gastropoda: Pleuroceridae) (다슬기, Semisulcospira libertina libertina의 난자형성과정에 관한 미세구조적 기재)

  • Kim, Eun-Kyoung;Lee, Jung-Sick
    • The Korean Journal of Malacology
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    • v.25 no.2
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    • pp.145-151
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    • 2009
  • The ultrastructural changes in germ cells during oogenesis of the melania snail, Semisulcospira libertina libertina have been investigated by light and electron microscopy. The ovary is located on the surface of the hepatopancreas in the spiral posterior region. The ovary exhibited greenish color in the gonadal mature season. The ovary was composed of a number of oogenic follicles. Oogenesis was divided into five stages with histological features: (1) oogonia, (2) previtellogenic, (3) initial vitellogenic, (4) active vitellogenic, and (5) mature stages. Oogonia were oval in shape, $4-6\;{\mu}m$ in diameter, and had a large nucleus. Previtellogenic oocytes were about $20\;{\mu}m$ in diameter and the cytoplasm reacted with hematoxylin in H-E satin. Initial vitellogenic stage, oocytes were $60-80\;{\mu}m$ in diameter, and small yolk granules of low electron density are scattered in the cytoplasm. Oocytes in the initial vitellogenic stage were connected with ovarian follicle by egg stalk. Active vitellogenic oocyte were $100-120\;{\mu}m$ in diameter. Electron density, size and quantity of yolk granules that are distributed in the cytoplasm have increased from the previous stage. Result of TEM observations, the oocyte contains well-developed Golgi complex, endoplasmic reticula and tubular mitochondria in the cytoplasm. Cytoplasm of mature oocyte was filled with proteinaceous yolk globules of high electron density. In this stage, the length of microvilli in the egg envelope was approximately $1.1\;{\mu}m$.

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Studies on Early Life History of the Korean Mandarin Fish, Siniperca scherzeri(Perciformes: Centropomidae) (쏘가리 Siniperca scherzeri 의 초기 생활사에 관한 연구)

  • Lee, Wan-Ok;Jang, Seon-Il;Lee, Jong-Yun
    • Korean Journal of Ichthyology
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    • v.10 no.1
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    • pp.1-10
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    • 1998
  • The early history of Korean mandarin fish, Siniperca scherzeri was studied to obtain some information required in aquaculture and reinforcement of natural population. During the period from June 1996 to July 1997, the mature adults of Siniperca scherzeri were collected from the middle Soyang Lake at Puksan - myon, Chunchon - shi, Kangwon - do, Korea. The eggs from females were obtained by injecting HCG or/and GnRH - a and fertilized by dry method in the laboratory. The fertilized eggs, measuring 1.70~2.10 mm in diameter and expanded to 2.20~2.66mm after absorption of water, were globosity, light orange yellow, separative, submergence and had one large oil globules of 0.5~0.7 mm. The blastodisc was formed in 1 hour and cleavage started in 1 hour 30 min. after fertilization, and the intervals of each stage of cleavage was about 50 min. at the water temperature of $21{\sim}24^{\circ}C$. Hatching occurred 131 hours 30 min. after fertilization and newly hatched larvae were 5.86~6.85 mm in total length(TL) and numerous stellate melanophores were distributed on the yolk and abdomen of caudal peduncle. The yolk was almost absorbed and the teeth development. 3 days after hatching, at 6.98~7.60 mm TL. The head spines and the teeth were largely developed and all fins were completely formed and became postlarva stage 15 days after hatching, at 10.10~12.90 mm TL. The body shape and the color pattern were similar to adult, 25 days after hatching, at 15.3~23.8 mm TL. In 5 months after hatching were reached at 154.10~175.02 mm TL and 49.32~82.67 g in body weight.

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Breeding of ‘Gommany’ Cultivar of Gomchwi with Disease Resistant, High Quality, and Yield (고품질 내병 다수성 곰취 신품종 ‘곰마니’ 육성)

  • Suh, Jong Taek;Yoo, Dong Lim;Kim, Ki Deog;Lee, Jong Nam;Kwon, Young Seok;Kim, Won Bae
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.625-629
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    • 2016
  • A new Gomchwi cultivar ‘Gommany’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. Petiole trichome existed, but light of leaf back did not. Density of leaf vein was 5 degree in a newly developed cultivar ‘Gommany’. Plant height, leaf length, leaf width and petiole length were 47.7, 16.3, 21.2, and 31.4 ㎝, respectively in the 3 year of growth characteristics. Plant size was also higher than that of ‘Gondalbi’. Bolting and flowering time were Aug. 5 and Sept. 10, respectively. In contrast, Bolting time of ‘Gondalbi’ showed 25 days quite earlier bolting time compared to ‘Gommany’, Which led to earlier flowering time. ‘Gommany’ showed higher number of leaves (138) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was 97% higher in ‘Gommany’ (1,575 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Gommany’ showed higher leaf thickness (0.69㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (24.3 ㎏/㎠) compared to ‘Gondalbi’ (23.0㎏/㎠). ‘Gommany’ showed higher resistance in the susceptibility of powdery mildew compared to ‘Gondalbi’. ‘Gommany’ cultivar was registered as a novel variety with number 90 in The Minister of the Korea Forest Service on December 2015.

A Hybrid Approach of Efficient Facial Feature Detection and Tracking for Real-time Face Direction Estimation (실시간 얼굴 방향성 추정을 위한 효율적인 얼굴 특성 검출과 추적의 결합방법)

  • Kim, Woonggi;Chun, Junchul
    • Journal of Internet Computing and Services
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    • v.14 no.6
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    • pp.117-124
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    • 2013
  • In this paper, we present a new method which efficiently estimates a face direction from a sequences of input video images in real time fashion. For this work, the proposed method performs detecting the facial region and major facial features such as both eyes, nose and mouth by using the Haar-like feature, which is relatively not sensitive against light variation, from the detected facial area. Then, it becomes able to track the feature points from every frame using optical flow in real time fashion, and determine the direction of the face based on the feature points tracked. Further, in order to prevent the erroneously recognizing the false positions of the facial features when if the coordinates of the features are lost during the tracking by using optical flow, the proposed method determines the validity of locations of the facial features using the template matching of detected facial features in real time. Depending on the correlation rate of re-considering the detection of the features by the template matching, the face direction estimation process is divided into detecting the facial features again or tracking features while determining the direction of the face. The template matching initially saves the location information of 4 facial features such as the left and right eye, the end of nose and mouse in facial feature detection phase and reevaluated these information when the similarity measure between the stored information and the traced facial information by optical flow is exceed a certain level of threshold by detecting the new facial features from the input image. The proposed approach automatically combines the phase of detecting facial features and the phase of tracking features reciprocally and enables to estimate face pose stably in a real-time fashion. From the experiment, we can prove that the proposed method efficiently estimates face direction.

A Quality-control Experiment Involving an Optical Televiewer Using a Fractured Borehole Model (균열모형시추공을 이용한 광학영상화검층 품질관리 시험)

  • Jeong, Seungho;Shin, Jehyun;Hwang, Seho;Kim, Ji-Soo
    • The Journal of Engineering Geology
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    • v.30 no.1
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    • pp.17-30
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    • 2020
  • An optical televiewer is a geophysical logging device that produces continuous high-resolution full-azimuth images of a borehole wall using a light-emitting-diode and a complementary metal-oxide semiconductor image sensor to provide valuable information on subsurface discontinuities. Recently, borehole imaging logging has been applied in many fields, including ground subsidence monitoring, rock mass integrity evaluation, stress-induced fracture detection, and glacial annual-layer measurements in polar regions. Widely used commercial borehole imaging logging systems typically have limitations depending on equipment specifications, meaning that it is necessary to clearly verify the scope of applications while maintaining appropriate quality control for various borehole conditions. However, it is difficult to directly check the accuracy, implementation, and reliability for outcomes, as images derived from an optical televiewer constitute in situ data. In this study, we designed and constructed a modular fractured borehole model having similar conditions to a borehole environment to report unprecedented results regarding reliable data acquisition and processing. We investigate sonde magnetometer accuracy, color realization, and fracture resolution, and suggest data processing methods to obtain accurate aperture measurements. The experiment involving the fractured borehole model should enhance not only measurement quality but also interpretations of high-resolution and reliable optical imaging logs.

Induction and RAPD Analysis of Mutant Plants by Chemical Mutagens in Gentiana axillariflora Leveille (큰용담 (Gentiana axillarifloa L,) 기내배양에서 화학돌연변이원 처리에 의한 돌연변이주 유기 및 RAPD 분석)

  • 임정대;김명조;유창연
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.2
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    • pp.89-94
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    • 2000
  • In order to induce the mutants of Gentiana axillariflora Leveille, nodes were cultured on Schenk and Hilderbrandt (SH) medium containing TDZ 2 mg/L, BAP 2mg/L, GA3 0.5 mg/L and 0.1 mg/L NAA and each mutagen of ethylmethanesulfate (EMS), colchicine, N-methyl-N-nitrosourea (MNU), and sodium azide (NaN$_3$) through filtration. Comparision of morphological characteristics and survival rate in each mutant plants differed depending on mutagen sources and their concentrations. When EMS were treated on nodes, the regenerated plants was thin and albino, regenerated shoots appeared 'erectoides type' and get twisted. The case of colchicine were treated on nodes, the survival rate was from 84% to 97% at ail concentration after 30days but the rate of survival was decreased about 50% at 200 $\mu$M after 60days. The treatment of NaN$_3$200 $\mu$M was not survived. The survival rate was extremely decreased in MNU treatment at 500$\mu$M, according to concentrations two types of leaf characteristic were obtained. Type I of leaf characteristic was modified from oblanceolate to oboid at leaf shape and type II of leaf characteristic was modified from light green to dark violet at leaf color. RAPD analysis was carried out to check the genetic modification of regenerated plants by mutagen treatments. Three polymorphic DNA fragments out of thirty-seven obtained by RAPDs were observed in regenerated plants using 5 decamer primers.

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Transmittance Properties of Fishing Lamp in Stick-held Dip Net Fishing Vessel for Pacific Saury (꽁치봉수망 집어등 불빛의 수중 투과 특성)

  • Jo, Hyun-Su;Kim, Doo-Nam;Cho, Young-Bok;Lee, Ju-Hee;Kim, Hyung-Seok;Yang, Won-Seok;An, Heui-Chun;Han, Jin-Seok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.40 no.1
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    • pp.23-28
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    • 2004
  • The transmittance properties of fishing lamp in stick-held dip net fishing vessel for Pacific saury was investigated during nighttime operations in the North Pacific on May 19 and 24, 2003. The incandescent lamps of red color (750W${\times}$100) and halogen lamps (750W${\times}$521) were used as a fishing lamp for gathering Pacific saury. The relative irradiance of red incandescent lamp and halogen lamp in the air showed peak in 1,052nm of wave length. However, the irradiance of halogen lamp below 600nm of wave length was higher than that of incandescent lamp. The relationship between underwater illuminance (Y) and water depth (X) of sunlight in the observation areas A (37$^{\circ}$ 11'N, 178$^{\circ}$ 46'W) and B (31$^{\circ}$ 11'N, 178$^{\circ}$ 01'E) is represented as follows; $Y=2572.2{\cdot}e^{-0.0721X},\;R^2=0.9915$ $Y=3312.4{\cdot}e^{-0.0619X},\;R^2=0.9837$ The distribution of underwater illuminance of observation areas A and B showed low value of 0.31x and 0.61x in 50m depth, respectively. In the fishing grounds of Pacific saury, the light intensity of distribution depth was above 0.51x.

Bermuda Grass Hay or Sorghum Silage with or without Yeast Addition on Performance and Carcass Characteristics of Crossbred Young Bulls Finished in Feedlot

  • Maggioni, Daniele;De Araujo, Jair Marques;Perotto, Daniel;Rotta, Polyana Pizzi;Ducatti, Taciana;Matsushita, Makoto;Silva, Roberio Rodrigues;Prado, Ivanor Nunes do
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.2
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    • pp.206-215
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    • 2009
  • This experiment was carried out to evaluate performance and carcass characteristics of 40 crossbred young bulls ($Zebu{\times}European$) finished in a feedlot under two roughage sources (Bermuda grass hay or sorghum silage) with or without the addition of yeast (Saccharomyces cerevisae). The bulls were 20 months old, their initial average weight was 356 kg and they were allocated into four groups of ten animals. The experimental diets were Bermuda grass, Bermuda grass+yeast, sorghum silage and sorghum silage +yeast. Animal performance and carcass characteristics were not influenced by roughage source or yeast addition. The average daily weight gain was 1.50 kg, dry matter intake (DMI) was 11.1 kg/d, DMI as percentage of liveweight was 2.60% and feed dry matter conversion was 7.70. The mean dressing percentage was 52.0% and hot carcass weight was 268 kg. Carcass conformation was classified between good-minus to good. Carcass length (137 cm), leg length (72.9 cm) and cushion thickness (26.6 cm) were not influenced by treatments. The average fat thickness was 3.80 mm and the Longissimus muscle area was 66.9 $cm^{2}$. The classification of color, texture and marbling were slightly dark red to red, fine and slight-minus to light-typical, respectively. The mean percentage of bone, muscle and fat in the carcass was 15.5%, 62.3% and 22.5%, respectively. Yeast addition increased ${\gamma}$-linolenic fatty acid (0.15 vs. 0.11%) deposition. Bermuda grass hay increased deposition of ${\alpha}$-linolenic (0.49 vs. 0.41%), arachidonic (2.30 vs. 1.57%), eicosapentaenoic (0.41 vs. 0.29%), docosapentaenoic (0.80 vs. 0.62%), docosahexaenoic (0.11 vs. 0.06%) and n-3 fatty acids, and reduced n-6: n-3 ratio in meat, when compared to sorghum silage treatments. The treatments had no effect on saturated fatty acids (49.5%), polyunsaturated fatty acids (11.8%), n-6 fatty acids (9.87%), n-3 (1.61%) and PUFA:SFA ratio (0.24). Monounsaturated fatty acid levels were higher on sorghum silage (40.7 vs. 37.7%). The addition of yeast caused higher n-6: n-3 ratio (7.28 vs. 5.70) than treatments without yeast.

Metamorphism of Anorthositic Rocks with Respect to Amphiboles in Hadong Area (각섬석류를 활용한 하동지역 회장암체의 변성작용에 관한 연구)

  • Kwak, Ji Young;Choi, Jin Beom
    • Journal of the Mineralogical Society of Korea
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    • v.28 no.3
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    • pp.221-231
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    • 2015
  • Precambrian Jirisan gneiss complex suffered retrograde metamorphism ranging from granulite facies to the amphibolite facies and/or greenschist facies. Intrusive anorthositic rocks in gneiss complex are influenced by late metamorphism. Mafic mineral in anorthositic rock composed mainly of amphiboles, which can anticipate the information about metamorphic conditions and metamorphic facies. Amphiboles from anorthositic rock show subhedral to anhedral in shape and mostly blueish green and/or green in colour in plane polarized light. Some of brownish amphiboles show zonal texture with brownish to blueish green in color from core to rim. Reaction parts in clinopyroxene which exchange with amphibole. It suggests retrograde metamorphism and/or alteration. Amphiboles composing anorthositic rocks can be classified into two types depending on the size and occurrence of amphibole. The first type is microcrystalline amphibole occurring matrix [Group I: ferrohornblende]. The second type is amphibole with 1 mm or larger in size, which is usually occurred in the boundary between opaque mineral and plagioclase [Group II: ferropargasite]. Electron microscopic analyses base on the $Al^{vi}$ composition in amphiboles suggest that the metamorphic pressure of anorthositic rock was low with 5 kbar or less. Ti compositional range in amphibole and representing hornblende+ plagioclase+garnet+biotite+chlorite mineral assemblage suggest that metamorphic facies of anorthositic rock is in amphibolite facies.