• Journal of Applied Biological Chemistry
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• 제66권
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• pp.29-38
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• 2023

Recent studies have highlighted the link between diseases and inflammation across our lifespan. Our sedentary lifestyle, high-calorie diet, chronic stress, chronic infections, and exposure to pollutants and xenobiotics, collectively intensify the course and recurrence of infections and inflammation in our bodies, promoting the prevalence of chronic diseases and aging. Given such phenomena and considering additional factors such as the frequency of prescription, and easy access to over-the-counter drugs, the need for anti-inflammatory therapeutics is ever-increasing. However, the readily available anti-inflammatory treatment option comes with a greater risk of side effects or high cost (biologics). Therefore in this growing competition of discovering and developing new potent anti-inflammatory drugs, we focused on utilizing the established knowledge of traditional medicine to find lead compounds. Since lead optimization is an indispensable step toward drug development, we applied this concept for the production of potent anti-inflammatory compounds achieved by structural modification of flavonoids. The derivative obtained through acetylation of myricetin, 3,3',4',5,5',7-hexaacetate myricetin, showed a greater inhibitory effect in the production of pro-inflammatory mediators such as nitric oxide, Prostaglandin E₂, and pro-inflammatory cytokines like interleukin-6, interleukin1β, in lipopolysaccharide-stimulated RAW264.7 mouse macrophage cells compared to myricetin. The increased potency of inhibition was in conjunction with an increased inhibitory effect on inducible nitric oxide synthase and cyclooxygenase-2 proteins. Through such measures, this study supports lead optimization for well-established lead compounds from traditional medicine using a simpler and greener chemistry approach for the purpose of designing and developing potent anti-inflammatory therapeutics with possibly fewer side effects and increased bioavailability.

• Clinical and Experimental Pediatrics
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• 제51권7호
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• pp.771-774
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• 2008

가족성 저칼륨성 주기성 마비는 골격근에 존재하는 ion channel의 장애로 인해 저칼륨혈증과 연관되어 나타나는 주기성 이완성 마비를 보이는 드문 유전 질환이다. 이 질환의 발생에 관여하는 유전자로는 골격근육의 1q31-32 염색체에 위치하는 칼슘 채널과 나트륨 채널의 alpha subunit을 encoding하는 CACNA1S gene과 SCN4A gene이 밝혀져 있다. 국내에서도 소아의 가족성 저칼륨성 주기성 마비가 수 예 보고된 바 있지만, 유전자 분석을 통해 변이가 확인된 예는 드물다. 이에 우리는 CACNA1S의 Arg1239His 변이에 의한 저칼륨성 주기성 마비로 진단된 12세 환아의 증례를 보고하는 바이다. 이 변이는 현재까지 알려진 CACNA1S의 변이 중 비교적 흔한 것으로 알려져 있으나, 국내에서는 보고된 바가 없다. 저자들은 경구 acetazolamide와 칼륨 복용, 유발인자를 회피할 것을 교육함으로써 이 환아를 치료했으며, 현재 환아는 주기성 마비의 빈도와 중증도의 개선을 보이며 삶의 질 역시 향상되었다.

• Journal of Nutrition and Health
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• 제40권7호
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• pp.630-638
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• 2007

This study was performed to examine the combined effects of L-carnitine and isoflavone supplementation on weight reduction and body fat distribution in overweight women. Overweight/obese women (body mass index > $23kg/m^2$) who were not diagnosed any type of diseases were included in this study and sixty subjects ($41.1{\pm}1.5$ years, $25.9{\pm}0.3kg/m^2)$ were randomly assigned to a placebo (n=30) or a supplement group (n=30, L-carnitine 300 mg+isoflavone 40 mg/day). We measured anthropometric parameters, abdominal fat distribution by computerizd tomography and blood components before and after the 12 week intervention period. After the 12 weeks of supplementation, subjects in L-carnitine and isoflavone supplement group showed a significant reduction of body weight (p < 0.001), body fat % (p < 0.05), and waist to hip ratio (p < 0.01) whereas placebo group did not show any changes. In a CT-scanned results, total fat area at L4 level was significantly reduced by 8.1% (p < 0.01) with the reduction of visceral fat area (-11.1%, p < 0.001) and subcutaneous fat area (-7.0%, p < 0.05) in the supplement group. The supplementation of L-carnitine and isoflavone showed the significant improvement of HDL-C (p < 0.01) and apoB (p < 0.05) concentrations, however, change values in those markers were not significant compared with those of the placebo group. In addition, a significant increase of adiponectin level (p<0.001) was observed in the supplement group after the intervention. The result of present study demonstrated that supplementation of 300 mg L-carnitine and 40 mg isoflavone per day fur 12 weeks can give beneficial effects on weight reduction and visceral fat accumulation. These potential antiobesity supplement can produce more favorable effects when combined with lifestyle modification.

• 한국임상약학회지
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• 제22권4호
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• pp.330-339
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• 2012

Background : Hypertension is treated with both lifestyle modification and pharmacotherapy. The Seventh Report of the Joint National Committee on Detection, Evaluation, and Treatment of High Blood Pressure (JNC-7), published in 2003, provides a streamlined management approach to hypertension for the primary care physician. The JNC-7 is the gold standard also in Korea. According to the JNC-7, special therapeutic considerations are recommended for high-risk individuals with compelling indications. The presence of compelling indications in any given patient should be considered when selecting specific pharmacotherapy to treat hypertension. However, in patients with compelling indications, it is unknown that hepatotoxicity is caused by Calcium Channel Blocker (CCB), one of 1st anti-hypertensive drugs. Now, the CCB is the most used 1st anti-hypertensive drug in Korea Therefore, we evaluated the changes in blood liver function parameters (ALT, AST, Total bilirubin, serum albumin) for the study group. Methods : We randomly collected and retrospectively analyzed Electronic Medical Record data (n=28,788) of patients, and who took calcium channel blockers(non-dihydropyridines; diltiazem, verapamil, dihydropyridines; amlodipine, barnidipine, benidipine, clinidipine, efonidipine, felodipine, isradipine, lacidipine, lercanidipine, nicardipine, nifedipine, nimodipine), with having liver function tests (LFTs) from July 1st 2009 to June 30th 2010 at the Seoul National University Hospital in Korea. Control groups are two antihypertensive agents: RAS blockade (ARB; candesartan, irbesartan, losartan, olmesartan, telmisartan, valsartan, ACE-I; cilazapril, enalapril, fosinopril, imidapril, perindopril, ramipril) and, Diuretics (loop; furosemide, torsemide, thiazide; hydrochlorothiazide[HCTZ], indapamide). Patients not having LFT results at these three standard points of time(baseline, during, medication, and after finishing medication) were excluded. The collected data were analyzed by using the SPSS (Version12.0) and Microsoft Excel (Version2007). Results : 711 patients who were treated CCB (297), RAS blockade (232) or Diuretics (182) monotherapy were selected for the study. In selected patients, liver damage degree(changes of each LFTs value) was higher in diuretics group than other groups, followed by RAS blockade and CCB. In diuretics group's was loop-diuretics group was higher than thiazide-diuretics group. In CCB group, Nondihydropyridine-CCB's damage degree was higher than Dihydropyrine-CCB's that. Conclusions : Despite the limitations due to the retrospective study, among patients with abnormal LFTs, the use of CCBs led to a less liver damage than other 1st anti-hypertensive agents. It can be recommended CCBs as one of the initial treatments of hypertension in patients with liver disease.

• 대한한의학회지
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• 제32권2호
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• pp.92-101
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• 2011

Purpose: To evaluate the effects on vision care program including traditional Korean treatment for elementary school students. Methods: 172 eyes of eighty-six older elementary students underwent a vision care program, including traditional Korean treatment, for 3 months between March 2010 and June 2010. The vision care program included health education, such as lifestyle modification, vision strengthening exercises and a course of treatment with traditional Korean methods. Traditional Korean treatment covered periocular acupuncture point stimulation, periauricular acupuncture point stimulation and herbal drug therapy. All patients were examined for visual acuity and auto-refraction and filled out a satisfactory questionnaire about ocular discomforts and quality of life before and after treatments. Results: Patients with mild myopia (-0.75~-2.00) were 61 (35.5%), and 46 (26.7%) were emetropia (${\pm}0.50$ or less). After treatment, uncorrected visual acuity in both eyes was $0.77{\pm}0.46$, which was lower than that before treatment ($0.82{\pm}0.51$), but corrected visual acuity was $0.98{\pm}0.21$, which was higher than that before treatment ($0.93{\pm}0.28 $). About the refractive change, the emetropia group showed a slightly myopic shift while the mild myopia group intended to reduce the myopic error. However, this was not statistically significant. The scales of eyeball pain or discomfort decreased significantly from $17.59{\pm}22.5$ before treatment to $10.28{\pm}17.80$ after treatment, and the scales of the quality of life increased from $1.85{\pm}1.25$ before treatment to $2.25{\pm}1.30$ after treatment. Most patients (90.7%) were satisfied with their treatment results. Conclusions: The vision care program including traditional Korean treatment for elementary school students was effective to improve corrected visual acuity in both eyes and delay the progression of school myopia. In addition, it helped to reduce eyeball pain and discomfort and ultimately to enhance quality of life. Long-term follow-up studies will be needed.

• 한국농촌간호학회지
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• 제7권1호
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• pp.5-17
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• 2012

Purpose: The objectives of this study were to investigate the prevalence of the metabolic syndrome (MetS) and to identify associated factors with MetS among rural residents. Methods: Data were collected from 1,196 subjects over aged 30 years by a self-administered questionnaire, physical measurement, and blood test in a rural area. The prevalence of MetS was determined by the criteria of the American Heart Association/National Heart, Lung, and Blood Institute (AHA/NHLBI) and 2005 the Korean society for the study of obesity. Results: The prevalence of MetS was 40.5% for men, 49.2% for women. The prevalence of risk factors of MetS was 57.4% for elevated blood pressure, 49.0% for low HDL-cholesterol, and 48.6% for abdominal obesity. Unemployment and higher Body mass index (BMI) were associated factors for MetS regardless of gender. And higher age and physical inactivity in women only increased the odds of the MetS. Especially, BMI was a strong risk factor of MetS in both men and women. Conclusion: The prevalence of metabolic syndrome was higher in a rural area. Therefore, health care providers should develop lifestyle modification program to increase physical activity level and to prevent the obesity among rural residents in order to decrease the prevalence of MetS.

• 대한지역사회작업치료학회지
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• 제8권3호
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• pp.1-12
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• 2018

목적 : 본 연구는 커뮤니티 케어 제도 내에서 생활기능향상 중심의 작업치료 모델을 개발하고자 기초 연구를 실시하였다. 연구방법 : 2018년 6월부터 동년 7월까지 생활기능향상 중심의 작업치료 모델을 개발하기 위하여 일본의 생활행위향상 매니지먼트(Management Tool for Daily Life Performance; MTDLP) 뿐만 아니라 스웨덴, 영국, 독일 등의 사례와 국내의 S 노인주간보호센터 및 I 보건소의 사례를 중심으로 커뮤니티 케어 관련 문헌을 조사하였다. 본 내용을 토대로 전문가 집단 회의를 거쳐 커뮤니티 케어 제도 내 작업치료 모델을 개발하였다. 결과 : 작업치료를 위한 평가를 시행하기 위하여 작업을 통한 건강 증진표, 흥미체크리스트, 생활기능향상을 위한 작업 목표, 생활기능 평가지, 일상생활시간 조사서(평일과 주말), 작업치료 종료 시 필요한 생활기능 인계지 등의 평가지를 개발하였다. 또한 작업치료사들이 이미 시행하고 있는 작업치료 중재 중 생활기능향상 프로그램, ICF모델 기반의 활동분석, 라이프 스타일 재설계 프로그램, 인지운동치료 프로그램, The Lee Silverman Voice Treatment (LSVT), 호스피스 완화 프로그램, 가정환경수정 프로그램 등을 중심으로 정리하였다. 결론 : 본 연구는 커뮤니티 케어 제도 내 작업치료의 실행을 위한 구체적 방안과 모델을 제시하였다. 작업치료가 클라이언트에게 반드시 필요한 하나의 전문 영역으로 자리매김하는데 필요한 한국형 생활기능향상 모델구축에 본 연구가 기초로 활용이 가능할 것으로 사료된다.

• 대한한의학회지
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• 제43권3호
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• pp.122-138
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• 2022

Objectives: This study was conducted to develop a Korean Medicine(KM) Clinical Practice Guidelines(CPG) of female infertility. We conducted this questionnaire survey to reflect the experiences of Korean Medicine doctors(KMD) and clinical field in Korea. Method: We sent a questionnaire survey to KMD belonging to the Association of Korean Medicine by e-mail. We received 665 responds, and analyzed the answers. Results: 51.2% of respondents knew the previously developed CPG of female infertility, and 18.3% actually used. 83.3% agreed about the necessity of CPG of female infertility, and 80.3% had practical use plan. 90.2% of respondents treated less than 5 infertility woman for a month. 22.7% of respondents treated 50% of patients with collaborative treatment of KM and Western medical treatments. The main age group of patients was '35~40 years'(54.7%), and the most common cause was unexplained infertility(61.7%). The most common pattern identification of female infertility patients was Kidney deficiency(55.4%). KMD used 'a combination of decoction of herbal medicine, acupuncture and moxibustion treatment' the most(43%), and 'a decoction of herbal medicine treatment alone' was next(35%). 84.2% conducted lifestyle modification education about diet, stress, exercise etc. Conclusion: We figured out Korean Medicine doctors' recognition about CPG of female infertility, preference of treatments, and also characteristics of patients visiting Korean Medical clinics to make a practical CPG reflecting clinical situation.

• 동아시아식생활학회지
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• 제17권3호
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• pp.351-363
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• 2007

Previously, we analyzed for nutrition knowledge and the use of nutrition knowledge in the everyday lives of students in order to develop nutrition education programs that focus on desirable behavior change. From this, we found that female students desired to participate in nutrition education more than male students, and regarding their concerns for nutrition education, 73.2% of the females and 50.0% of the males displayed interest in 'obesity and the regulation of body weight'. Therefore, this study showed female students give more attention to the obesity and the regulation of body weight than male students(p=.000<.001). In addition, female students had higher interests($8.63{\pm}1.67$) than male students ($7.45{\pm}2.03$) in nutrition knowledge(p=.000<.001). By investigating the use of nutrition knowledge in everyday life, our research indicated that the actual use of nutrition knowledge was less. To encourage students to persue dietary lives addressing the concerns confirmed above, the following needed to occur. 1) Provide them nutrition information for the main processed foodstuffs encountered when dining out(breads, cakes, cookies, and carbonated beverage). 2) Teach them to read food nutrition labels. 3) Help them find a lifestyle connection through lasting self-management methods and the generation of social support. Accordingly, this required developing effective and practical nutrition education programs that considered regional characteristics and gender differences. The most important factors considered during nutrition education program development were the need for motivation and ongoing education by stage of change, rather than temporal education through specific problem analysis, in order that those being educated may bring about a change of behavior by themselves. Therefore, from this study, we have suggested the use of multilateral operating strategies for successful nutrition education. In addition the phase model of behavior change should be applied. Our programs were aimed at self owned nutritional management so that students could master their own methods for acquiring skills and enjoying dietary life. The research may be summed up as follows. First, the purpose of education at the recognition stage of change was to attempt motivation for nutrition improvement, by analyzing the problems such as food buying habit and the main purchasing viewpoints when dining out. Second, the purpose of education at the action stage of change was to help students acquire of concrete methods for behavior modification by linking the program to their home as well as to teachers with various activities that suited the situation at school. This was done by analyzing the processes and decisions pertaining to dining out the main processed foodstuffs and principal components, etc. through data and experimental practice. Third, the purpose of education for changing of habits and values, or the maintenance stage, was to investigate the various reasons that undesirable behaviors were induced, and then determine a lasting self-management method as well as how to generate social support. If the nutrition education program developed in this study is utilized on site, someone in the primary role as the nutrition educator and trained specifically in nutrition, can help induce the health promotion in the community as well as lasting dietary management, by executing a link with families in parallel with educating teaching staff and students' parents. In addition, this program can playa role in the government policies related to the health promotion for our youth who are the foundation of our nation and who can enhance our national competitive power.

• 생명과학회지
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• 제31권8호
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• pp.699-709
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• 2021

생활습관 개선을 통한 근육량향상은 대사 증후군의 위험을 줄이는 것으로 보고되었다. 본 연구에서는 갈조류인 고리매(Scytosiphon lomentaria) 에탄올 추출물이 골격근 성장억제조절 단백질인 마이오스타틴 신호전달을 억제하는지와 고 급식으로 유도된 비만 제브라피시의 대사 항상성에 대한 효과를 확인하였다. 고리매 에탄올 추출물(10 ㎍/ml)은 pGL3- (CAGA) 12-루시퍼라제 분석에서 마이오스타틴(1 nM/ml) 신호를 완전히 차단하였다. 또한 웨스턴 블롯 분석에서 마이오스타틴 신호를 차단하여 Smad2 인산화가 억제되는 것을 확인하였다. 제브라피쉬의 치어에 대한 연구는 고 급식 대조군 그룹의 체내 포도당 농도는 정상 급식 대조군 그룹보다 유의하게 높았지만, 12.5 ug의 고리매 에탄올을 처리한 고 급식 그룹과 18.75 ug의 고리매 에탄올로 처리한 고 급식 그룹의 체내 포도당 수준은 정상 급식 대조군 그룹과 유사하였다. 고 급식 그룹의 GLUT2 유전자의 mRNA 발현 수준은 정상 급식 대조군 그룹에 비해 현저히 낮았다. 하지만, 고리매 에탄올 추출물을 처리한 실험군 그룹의 GLUT2 유전자의 발현은 정상 급식 대조군 그룹의 GLUT2 유전자의 발현과 거의 유사하게 나타났다. 그러므로 고리매 에탄올 추출물은 GLUT2유전자의 발현 조절을 통하여 체내 포도당 조절이 가능함을 보여준다. 본 연구의 결과는 고리매 에탄올 추출물이 대사 증후군 치료에 도움을 주는 소재 및 마이오스타틴 억제제로서의 가능성을 시사합니다.