• 펄프종이기술
• /
• 제43권3호
• /
• pp.35-42
• /
• 2011

In this study, ground calcium carbonate (GCC) was modified by Layer-by-Layer (LbL) multilayering with polyelectrolytes. Cationic polyacrylamide (C-PAM) and poly sodium 4-styrene sulfonate (PSS) were used as cationic and anionic polyelectrolytes to modify GCC. The characteristics of the modified GCC were examined in terms of zeta potential and particle size with the addition level of polyelectrolyte and layer number. The GCC could form an assembly of cationic and anionic polyelectrolytes through consecutive adsorption process. The zeta potential of the modified GCC moved toward the cationicity and reached the plateau with the increase of the addition level of C-PAM. With layering of anionic PSS, the GCC had the negative charge. The particle size was dependent on the zeta potential. It was also observed by optical microscope. As the PSS was in the presence of the outermost layer, the GCC showed the better dispersability. It indicated that the surface charge and particle size can be controlled by adjusting the addition level of polyelectrolyte and the layer number.

• Molecules and Cells
• /
• 제25권4호
• /
• pp.566-571
• /
• 2008

Calcineurin (Cn) is a calcium/calmodulin-dependent serine/threonine protein phosphatase that has diverse functions in different cell types and organisms. We screened proteins interacting with the C. elegans CnA homolog, TAX-6, by the yeast two-hybrid system. CNP-3 (Calcineurin interacting protein-3) is a novel protein that physically interacts with the catalytic domain of TAX-6. It is strongly expressed in the nuclei of intestine, hypodermis, dorsal uterine regions and spermatheca. Expression begins around the 60-cell stage and proceeds during all larval stages and the adult. To elucidate the biological function of cnp-3 we isolated a cnp-3 deletion mutant. Since CNP-3 binds CnA, we looked at factors associated with calcineurin loss-of-function mutants, such as brood size, body size, serotonin- and levamisole-mediated egg-laying behavior. The cnp-3(jh145) single mutant had no gross defects compared to wild-type animal. However, the phenotypes of the double mutants, tax-6(p675);cnp-3(jh145) and cnb-1(jh103);cnp-3(jh145), were more severe in terms of brood size, body size and serotonin-mediated egg-laying defects than tax-6(p675) and cnb-1(jh103), respectively. These results suggest that dysfunction of cnp-3 enhances certain calcineurin loss-of-function phenotypes in C. elegans.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권8호
• /
• pp.1110-1118
• /
• 2018

Objective: This study was intended to identify genes positively selected in Thoroughbred horses (THBs) that potentially contribute to their running performances. Methods: The genomes of THB and Jeju horses (JH, Korean native horse) were compared to identify genes positively selected in THB. We performed cross-population extended haplotype homozygosity (XP-EHH) and cross-population composite likelihood ratio test (XP-CLR) statistical methods for our analysis using whole genome resequencing data of 14 THB and 6 JH. Results: We identified 98 (XP-EHH) and 200 (XP-CLR) genes that are under positive selection in THB. Gene enrichment analysis identified 72 gene ontology biological process (GO BP) terms. The genes and GO BP terms explained some of THB's characteristics such as immunity, energy metabolism and eye size and function related to running performances. GO BP terms that play key roles in several cell signaling mechanisms, which affected ocular size and visual functions were identified. GO BP term Eye photoreceptor cell differentiation is among the terms annotated presumed to affect eye size. Conclusion: Our analysis revealed some positively selected candidate genes in THB related to their racing performances. The genes detected are related to the immunity, ocular size and function, and energy metabolism.

• 동아시아식생활학회지
• /
• 제20권1호
• /
• pp.187-192
• /
• 2010

The study investigated the effect of rice particle size on the physicochemical properties of soybean porridge prepared at different pH. Redness, yellowness and viscosity of soybean porridge increased according to the rice particle size. Protein content of soybean porridge was increased 2-fold, compared to that of rice porridges. Total amino acid content of soybean porridge was 1,821.2 mg/100 g and that of rice was 1,719.1 mg/100 g. Lysine and threonine contents of limited amino acid of soybean porridge were increased. Half grain rice soybean porridge exhibited the highest sensory scores. The results indicated that half grain size soybean porridge is preferred, both physicochemically and nutritionally.

• 펄프종이기술
• /
• 제40권3호
• /
• pp.59-64
• /
• 2008

The mechanical properties of WPC(wood plastic composite) should effected with the size of wood particle size and also characteristics of wood particles. In this paper, WPC were prepared with various size of wood particles and coupling agent and the mechanical properties were evaluated. The smaller size of wood particle were used for WPC, the higher properties of WPC in tensile strength and breaking elongation were obtained. The smaller amount of wood particle were used for WPC, the higher properties of WPC in tensile strength and breaking elongation were obtained.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권3호
• /
• pp.420-427
• /
• 2015

It is necessary to understand the cellular uptake and cytotoxicity of food-grade delivery systems, such as ${\beta}$-lactoglobulin (${\beta}$-lg) nanoparticles, for the application of bioactive compounds to functional foods. The objectives of this study were to investigate the relationships between the physicochemical properties of ${\beta}$-lg nanoparticles, such as particle size and zeta-potential value, and their cellular uptakes and cytotoxicity in Caco-2 cells. Physicochemical properties of ${\beta}$-lg nanoparticles were evaluated using particle size analyzer. Flow cytometry and confocal laser scanning microscopy were used to investigate cellular uptake and cytotoxicity of ${\beta}$-lg nanoparticles. The ${\beta}$-lg nanoparticles with various particle sizes (98 to 192 nm) and zeta-potential values (-14.8 to -17.6 mV) were successfully formed. A decrease in heating temperature from $70^{\circ}C$ to $60^{\circ}C$ resulted in a decrease in the particle size and an increase in the zeta-potential value of ${\beta}$-lg nanoparticles. Non-cytotoxicity was observed in Caco-2 cells treated with ${\beta}$-lg nanoparticles. There was an increase in cellular uptake of ${\beta}$-lg nanoparticles with a decrease in particle size and an increase in zeta-potential value. Cellular uptake ${\beta}$-lg nanoparticles was negatively correlated with particle size and positively correlated with zeta-potential value. Therefore, these results suggest that the particle size and zeta-potential value of ${\beta}$-lg nanoparticles play an important role in the cellular uptake. The ${\beta}$-lg nanoparticles can be used as a delivery system in foods due to its high cellular uptake and non-cytotoxicity.

• Journal of Microbiology
• /
• 제44권5호
• /
• pp.530-536
• /
• 2006

Bacteriophage P4, a satellite phage of coliphage P2, is a very useful experimental tool for the study of viral capsid assembly and cos-cleavage. For an in vitro cos-cleavage reaction study of the P2-P4 system, new shortened and selectable markers containing P4 derivative plasm ids were designed as a substrate molecules. They were constructed by swapping the non-essential segment of P4 DNA for either the kanamycin resistance (kmr) gene or the ampicillin resistance (apr) gene. The size of the genomes of the resulting markers were 82% (P4 ash8 delRI:: kmr) and 79% (P4 ash8 delRI:: apr) of the wild type P4 genome. To determine the lower limit of genome size that could be packaged into the small P4-size bead, these shortened P4 plasmids were converted to phage particles with infection of the helper phage P2. The conversion of plasmid P4 derivatives to bacteriophage particles was verified by the heat stability test and the burst size determination experiment. CsCl buoyant equilibrium density gradient experiments confirmed not only the genome size of the viable phage form of shortened P4 derivatives, but also their packaging into the small P4-size head. P4 ash8 delRI:: apr turned out to be the smallest P4 genome that can be packaged into P4-sized head.

• 동아시아식생활학회지
• /
• 제7권2호
• /
• pp.159-165
• /
• 1997

The primary objective of this study was to learn the effect of various sugar particle size and level on cookie spread, The effect of sugar particle size and level on sugarsnap cookie spread was studied. Three different particle sized sugars; powered sugar, granulated sugar and sanding sugar, were used for the cookie test baking with five different sugar levels; 30, 45, 60, 75 and 90% based on the weight of flour. In mixing process, 5 minutes of creaming time was used for cream making and then the specific gravity of cream was measured on the basis of each different sugar particle size and level. In the result, the specific gravity of cream was influenced by sugar particle size and sugar level. However, the specific gravity of cream had no influence on cookie dough specific gravity. Cookie spread was influenced by sugar particle size and sugar level. Greater cookie spread was obtained by decreasing sugar particle size and increasing sugar level resulted in increased spread. Therefore, selection of suitable sugar particle size and its use level can be factors in controlling spread and imparting desired packaging characteristics.

• 생명과학회지
• /
• 제7권4호
• /
• pp.358-360
• /
• 1997

대두 종자크기에 대한 QTL의 consistency을 알아보기 위하여 F$_{2}$, F$_{3}$ 세대에서의 분석결과를 요약하면 댜음과 같다. 세 개의 마크 OTL09a, OPM07a, OPAC12 가 F$_{2} 세대에서 종자 크기에 대한 QTL과 높은 유의성을 나타내었고, F$_{3}$ 세애데는 네 개의 마크 OTL09a, OPG19a, OPL09b, OPP11가 유의성을 나타내었다. 두 개의 마크 OPL09a, OPL09b 가 두 세대에서 유의성을 나타애어 종자 크기에 대한 QTL의 consistency가 인정되었다.

• 대한가정학회지
• /
• 제35권3호
• /
• pp.221-230
• /
• 1997

This study attempts to examine the average number of stored items of apartment residents composed of 4 family members and to compare the quantitative differences of the stored items according to the apartment size and the stage of life-cycle. The finding shows that the number of items stored in apartments increases as the size of apartments and the stag of life-cycle of husband and wife living in the apartments of more than 180$m^2$ increase approcimately twice, compared to those in the apartments of less than 83$m^2$. Therefore, the storage space in apartments should be designed more effectively considering the change of the stored items in relation to of the apartment size and their stage of life-cycle. This study suggests the specific design of the built-in closets for clothings, sheets, accessories and shoes based on the number of the items.