• Journal of Pharmaceutical Investigation
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• 제37권5호
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• pp.315-321
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• 2007

The purpose of the present study was to evaluate the bioequivalence of two lercanidipine hydrochloride tablets, Zanidip tablet (LG Life Sciences Ltd., Korea, reference drug) and Samchundang Lercanidipine tablet 10 mg (Sam Chun Dang Pharm. Co. Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (amlodipine maleate) to human serum, serum samples were extracted using hexan-isoamyl alcohol (100:1, v/v). Compounds were analyzed by liquid chromatography/tandem mass spectrometry. This method showed linear response over the concentration range of 0.05-20 ng/mL with correlation coefficient of 0.9999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ng/mL which was sensitive enough for pharmacokinetic studies. Thirty healthy male Korean volunteers received each medicine at the lercanidipine hydrochloride dose of 20 mg in a $2\;{\times}\;2$ crossover study. There was a one-week washout period between the doses. Serum concentrations of lercanidipine were monitored by an LC/MS/MS fer over a period of 24 hr after the administration. $AUC_t$ (the area under the serum concentration-time curve from time 0 to 24 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (the maximum serum drug concentration) and $T_{max}$ (the time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters, indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Samchundang Lercanidipine/Zanidip were log 0.9505-log 1.2258 and log 0.9987-log 1.2013, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Samchundang Lercanidipine tablet 10 mg and Zanidip tablet are bioequivalent.

• 생태와환경
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• 제45권4호
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• pp.420-443
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• 2012

An Individual-Based Model (IBM) was developed by employing natural and toxic survival rates of individuals to elucidate the community responses of benthic macroin-vertebrates to anthropogenic disturbance in the streams. Experimental models (dose-response and relative sensitivity) and mathematical models (power law and negative exponential distribution) were applied to determinate the individual survival rates due to acute toxicity in stressful conditions. A power law was additionally used to present the natural survival rate. Life events, covering movement, exposure to contaminants, death and reproduction, were simulated in the IBM at the individual level in small (1 m) and short (1 week) scales to produce species abundance distributions (SADs) at the community level in large (5 km) and long (1~2 years) scales. Consequently, the SADs, such as geometric series, log-series, and log-normal distribution, were accordingly observed at severely (Biological Monitoring Working Party (BMWP<10), intermediately (BMWP<40) and weakly (BMWP${\geq}50$) polluted sites. The results from a power law and negative exponential distribution were suitably fitted to the field data across the different levels of pollution, according to the Kolmogorov-Smirnov test. The IBMs incorporating natural and toxic survival rates in individuals were useful for presenting community responses to disturbances and could be utilized as an integrative tool to elucidate community establishment processes in benthic macroin-vertebrates in the streams.

• 농촌지도와개발
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• 제23권1호
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• pp.27-37
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• 2016

This paper estimated the demand function of the cuts of pork by using the consumer panel and the weather data from the Korea Meteorological Administration with a log-log model and AIDS model. The results show that the price elasticity of demand for cuts of pork is high in the sequence of arm shoulder, leg, special cuts, tenderloin, blade shoulder. In contrast, spare rib, belly and loin are classified as low price elasticity of demand. The income elasticities of demand for leg and blade shoulder are 11% and 7.6% respectively. The loin is classified as inferior goods, because demand decreases when income increases. The results also demonstrate show that the demand increases highly in the sequence of loin, arm shoulder, spare rib, belly if housewife is older, and the demand of belly increases when family number increases. Belly substitutes every cut except spare rib, and tenderloin substitutes special cuts. Spare rib, on the other hand, does not substitute other cuts. In addition, job status, family member, husband job, purchasing place, consumer characteristic, eating-out times, purchasing time, and weather are statistically significant.

• Preventive Nutrition and Food Science
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• 제13권2호
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• pp.104-111
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• 2008

As a way to account for the variability of the primary model parameters in the secondary modeling of microbial growth, three different regression approaches were compared in determining the confidence interval of the temperature-dependent primary model parameters and the estimated microbial growth during storage: bootstrapped regression with all the individual primary model parameter values; bootstrapped regression with average values at each temperature; and simple regression with regression lines of 2.5% and 97.5% percentile values. Temperature dependences of converted parameters (log $q_o$, ${\mu}_{max}^{1/2}$, log $N_{max}$) of hypothetical initial physiological state, maximum specific growth rate, and maximum cell density in Baranyi's model were subjected to the regression by quadratic, linear, and linear function, respectively. With an advantage of extracting the primary model parameters instantaneously at any temperature by using mathematical functions, regression lines of 2.5% and 97.5% percentile values were capable of accounting for variation in experimental data of microbial growth under constant and fluctuating temperature conditions.

• 동아시아식생활학회지
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• 제27권4호
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• pp.473-476
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• 2017

사회관계망서비스는 많은 분야에서 활용이 되고 있으며, 특히 식품접객업소를 이용한 소비자들이 맛이나 품질, 위생상태에 대한 평가를 사회관계망서비스에 많이 남기고, 이를 바탕으로 다른 소비자들이 이용할 식품접객업소를 선택하는 경향이 뚜렷해지고 있다. 하지만, 이러한 사회관계망서비스상의 식품접객업소에 대한 소비자의 평가와 실질적인 위생상태의 연관성에 대한 평가는 아직 이루어지지 않았다. 따라서, 본 연구는 사회관계망서비스 상의 식품접객업소에 대한 평가와 실제 식품접객업소의 음식 위생 상태와의 연관성을 규명하기 위하여 수행되었다. 국내 대표 포털사이트를 이용하여 식품접객업소를 선정하였으며, 동일 업종에 따라 대중의 평가가 좋은 업소 6곳과 좋지 않은 업소 6곳을 최종 선정하였다. 각 업소에서 제공하는 주 요리 및 반찬에 대한 일반 세균수 및 위생지표세균(대장균군, 대장균)에 대한 오염도 조사를 실시하였다. 일반세균의 검출률과 세균수는 소비자 평가에 따른 차이가 없었다. 하지만, 대장균군의 경우, 소비자 평가가 좋은 업소에서 평균 2.3 log CFU/g(검출률 62.5%), 평가가 좋지 않은 업소에서 평균 3.2 log CFU/g(검출률 70.8%)으로 나타나, 평가가 나쁜 업소에서 오염도가 높은 것을 알 수 있었다. 대장균의 경우도 평가가 나쁜 업소들이 검출률(25%)과 평균 세균수(0.8 log CFU/g)가 평가가 좋은 업소들(검출률: 8.3%, 평균 세균수: 0.5 log CFU/g)보다 높게 나타났다. 종합적으로, 본 연구를 통해 사회관계망서비스 상의 소비자 평가와 식품접객업소의 위생상태가 연관성이 있음을 알 수 있으므로, 이러한 결과를 바탕으로 국내 식품접객업소의 위생상태 점검 및 관리 시 사회관계망서비스 상의 소비자 평가 자료를 활용한다면 좀 더 효율적으로 위생상태 점검 및 관리가 이루어질 수 있을 것으로 판단된다.

• Journal of the Korean Data and Information Science Society
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• 제21권4호
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• pp.747-756
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• 2010

최근의 전 세계적인 경기 침체에 따라 산업계 전반에 관한 연구가 활발히 진행되고 있다. 본 연구에서는 신용보증기금에 등록된 중소기업들의 자료에 대하여 생존 분석을 이용하여 생존율을 추정하였다. 또한 중소기업의 자산규모와 업종에 따라 건설업과 타 업종으로 구분하여 생존율에 관한 동향을 비교분석하였다. 이때 생존율은 생명표에 의해 구하였으며, 업종별 생존율의 차이는 로그순위 검정과 윌콕슨의 검정통계량을 사용하여 분석하였다. 실험 결과 중소기업의 자산규모가 10억 이상이 가장 높았으며, 1억 미만, 1억에서 10억 미만은 비슷한 생존율을 보였다. 업종별로는 도소매업과 서비스업이 경공업과 중공업, 건설업에 비하여 생존율이 높았으며 건설업의 경우 생존율이 가장 낮음을 알 수 있었다. 또한 대부분의 중소기업들은 시간이 지날수록 위험률이 상승하는 추세를 보였다.

• 한국축산식품학회지
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• 제29권1호
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• pp.47-54
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• 2009

Predictive modeling was applied to study the growth of microorganisms related to spoilage in frankfurter sausage containing various levels of dietary fiber (0, 1, 2, and 3%) from rice bran and to estimate its shelf-life. Using the Baranyi model, total viable cells, anaerobic and psychrotrophic bacteria were measured during 35 days of cold storage ($<4{\pm}1^{\circ}C$). The lag times (LT) demonstrated by control and treatment groups were 6.28, 623, 6.24, and 6.25 days, respectively. The growth rate of total viable cells in each group were 0.95, 0.91, 0.92, and 0.91 (Log CFU/g/day), respectively. The anaerobic and psychrotrophic bacteria had lower initial ($y_0$) and maximal bacterial counts ($y_{max}$) than total viable cells. Also, the anaerobic and psychrotrophic bacteria possessed lower growth rate and longer lag time than total viable cells. The estimated shelf-life of frankfurter containing rice bran fiber by the growth rate of total viable cells was 7.8, 7.9, 7.9, and 7.7 days, respectively. There were no significant differences in shelf-life as a function of fiber content. In other words, the addition of dietary fiber in sausage did not show the critically hazardous results in growth of microorganism. The 12 predictive models were then characterized by high $R^2$, and small RMSE. Furthermore, $B_f$ and $A_f$ values showed a very close relationship between the predictive and observed data.

• 한국인터넷방송통신학회논문지
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• 제15권1호
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• pp.229-235
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• 2015

ICT 힐링 플랫폼은 생체신호 및 생활습관 등의 정보를 기반으로 한 질환조기 경보를 목표로 하는 개념으로서 만성질환 예방을 목적으로 한다. ICT(Information & Communication Technology) 힐링플랫폼의 목표는 개인이 주도하는 건강관리를 위해 여러 건강 관련 서비스 기관들(병원, 피트니스센터, 건강검진센터, 개인건강장치 등)에 산재되어 있는 개인 건강 정보를 개방화하여 개인 단말장치로 모으고, 분석 플랫폼 및 Open API를 제공하여 다양한 부가 서비스들을 활성화하는데 있다. 본 논문에서 제안하는 개인화된 힐링 데이터 접근을 위한 개방형 게이트웨이 프레임워크인 HPAdapter (Healing Platform Adaptor)는 ICT 힐링플랫폼의 데이터 중계를 위해 EMR(Electronic health record), 한방의학, 라이프로그, 웰니스, 만성질활, 피트니스 등 다양한 개인 건강관련 데이터 공급자와 서비스 공급자 사이를 연결해 개인화된 힐링 데이터를 중계하는 소프트웨어 엔진을 뜻한다. 설계된 HPAdaptor는 데이터 및 서비스 공급자 연동을 통해 힐링 레코드 저장소, 모바일 플랫폼 및 분석 플랫폼 등 데이터를 필요로 하는 서비스 혹은 플랫폼의 중계참조 모델로 활용할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1604-1613
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• 2018

Lactobacillus rhamnosus GG (LGG) is a probiotic commonly used in fermented dairy products. In this study, RNA-sequencing was performed to unravel the effects of acid stress on LGG. The transcriptomic data revealed that the exposure of LGG to acid at pH 4.5 (resembling the final pH of fermented dairy products) for 1 h or 24 h provoked a stringent-type transcriptomic response wherein stress response- and glycolysis-related genes were upregulated, whereas genes involved in gluconeogenesis, amino acid metabolism, and nucleotide metabolism were suppressed. Notably, the pilus-specific adhesion genes, spaC, and spaF were significantly upregulated upon exposure to acid-stress. The transcriptomic results were further confirmed via quantitative polymerase chain reaction analysis. Moreover, acid-stressed LGG demonstrated an enhanced mucin-binding ability in vitro, with 1 log more LGG cells (p < 0.05) bound to a mucin layer in a 96-well culture plate as compared to the control. The enhanced intestinal binding ability of acid-stressed LGG was confirmed in an animal study, wherein significantly more viable LGG cells (${\geq}2log\;CFU/g$) were observed in the ileum, caecum, and colon of acid-stressed LGG-treated mice as compared with a non-acid-stressed LGG-treated control group. To our knowledge, this is the first report showing that acid stress enhanced the intestine-binding ability of LGG through the induction of pili-related genes.

• 대한예방한의학회지
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• 제27권1호
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• pp.89-98
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• 2023

Objectives : This study collected sleep information by wearable device in the Korean medicine Daejeon citizen cohort (KDCC). It was measured based on the sleep record information measured by wearing a Fitbit, and the possibility of clinical use was examined for compliance with objective sleep collection. Based on compliance, the possibility of clinical use was examined. Methods : After surveying personal information and PSQI(Pittsburgh Sleep Quality Index), sleep information was collected by Fitbit for 14 days. Compliance was measured based on sleep record information by Fitbit. Compliance was analyzed by sex, age, BMI(Body Mass Index), and sleep group(deep/poor). Results : The number of participants was 730, and the compliance was 94.3%, and the compliance group was 675(92.5%). The age of the participants varied from 30 to 60 years old, and the average age was 46±6.7 years. There were 218 males and 512 females. Young people have high compliance. Males are more compliance than females. As the BMI score decreased in the 30s, the compliance was higher. The underweight group in all age groups had 100 compliance. The underweight group was all female. The low compliance groups were that 30 years males (obesity level2), 50 years females (overweight group), and 50 years females (obesity level2). There was no significant difference in compliance between deep sleep group and poor sleep group. In deep sleep group, females showed higher compliance. In poor sleep group, males showed higher compliance. The average duration of Fitbit usage among participants was 20.1 days. The compliant group wore the device for an average of 21.3 days, while the non-compliant group wore it for only 5.2 days. Of the compliant group, 86.9% (73.8% of all participants) continued to wear the Fitbit after the recommended 14-day period, and 50.8% wore it for more than 20 days. Conclusions : This study showed the possibility of adaptation for wearing a Fitbit for collecting objective sleep information. It is judged that the compliance is high because it was worn for more than 13.2 days out of the 14 days required. It is considered meaningful because the compliance was measured based on the sleep information by Fitbit, not the questionnaire. As the data on objective sleep time is collected automatically, we believe that the burden on participants after the study period is not significant for a certain period. Compliance may be even higher for cohorts related to illnesses and with doctor's orders, rather than for the general population.