• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.45-53
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• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.

• Journal of IKEEE
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• v.23 no.4
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• pp.1208-1217
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• 2019

The life-cycle prediction of the sub-module which is the unit system of MMC is very important from the viewpoint of maintenance and economic feasibility of HVDC system. However, the life-cycle prediction that considers only the type, number and combination of parts is a generalized result that does not take into account the operating condition of the sub-module, and may significantly differ from the life-cycle of the actual one. Therefore, we design a fault tree for the purpose of reflecting the operation characteristics of the full-bridge sub-module and apply the MIL-HDBK-217F to the failure rate of the basic event to predict the life-cycle of the full-bridge sub-module. It compares the life-cycle expectancy of the conventional failure rate analysis with the proposed fault-tree analysis and compares the lifetime according to whether the redundancy of the full-bridge sub-module is considered.

• The Journal of the Korea Contents Association
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• v.12 no.5
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• pp.70-80
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• 2012

The marine life culture contents added with fun and learning factors are created in a 3D space, and the development of augmented reality contents concerning marine life resources in islands and the utilization method of experience-based learning are proposed. As a WYSIWYG-based authoring tool, an augmented reality authoring tool was made to easily use a authoring tool through a node structure and drag & drop. Marine life contents add the animation effect through a marker and event factors such as the change of modeling data, and also, they support real experience-based learning with the narration of marine life. Based on around 50 species of marine animals augmented reality contents, a marine animal AR book can be utilized as a textbook for elementary school classes, and as a 3D image education utilizing augmented reality, it enhances a learning effect by allowing realistic observation, various ways of thinking, and the maximum flow.

• The Journal of the Korea Contents Association
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• v.22 no.5
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• pp.503-517
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• 2022

This study was to investigate the social workers' psycho·social experiences and roles during the end-of-life care process in elderly long-term care facilities. As a result of data analysis through in-depth interviews, social workers experienced great exhaustion and burnout due to frequent death experiences during the end-of-life care process, and expressed regret for not being able to provide better service for the deceased and longing for loved ones. And in the event of a sudden death, social workers would undergo criticism and complaints. The main roles of social workers during end-of-life care were to contact and communicate with family members when signs of death appeared and support them after the death. The necessity of standardized manuals and education for end-of-life care, a recharging program and support group to prevent burnout of social workers, and a legal safety net for emergency preparedness and emergency measures were suggested. The necessity of death preparation education, hospice care, and advance medical directive was also emphasized for the dignified death of the elderly.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.5
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• pp.1123-1129
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• 2015

Macro(automatic hunting) of mobile game is a program that touch the screen by defined rules like a game bot in PC online games, and it is used by make various ways like android application or windows application program. This gives honest users deprivation and make to lose their interest. Finally they would leave the game and gradually game life would be shorten. Although many studies to prevent these problems in PC online game are conducted, applying mobile game to PC's way is difficult because mobile games are limited to use the network and device performance is different with PC. In this paper, we propose a framework for macro detection by using the touch event information. A touch event on the mobile game is a necessary control command to the game. Because macro touches the screen with the same pattern, there is a difference between normal user's behavior and macro's operation. In mobile games that casual games are mostly, Touch event is the best difference that identify normal user against macro for a short period of time. As a result of detecting macros used in real mobile game by using the proposed framework it showed 100% accuracy and 0% false positive rate.

• Korean Journal of Biological Psychiatry
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• v.8 no.2
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• pp.251-257
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• 2001

Backgrounds : Gamma band oscillatory activity is considered to be related to cognitive functions and illustrates that the concept of event-related oscillations bridges the gap between single neurons and neural assemblies. An event-related gamma oscillation is the time-locked responses of specific frequency, and can be identified by computing the amplitude frequency characteristics of the averaged event-related potentials(ERPs) after stimulation. Objectives : We purposed to present experimental paradigm to investigate ${\gamma}$-band oscillation activities from the recording of ERPs by using auditory oddball paradigm and investigate the difference of ${\gamma}$-band activity between schizophrenia and normal controls. Methods : The ERPs resulting from auditory stimuli with oddball paradigm in a group of schizophrenics(n=11), and also a group of age-, sex-, and handedness matched normal controls, were recorded by 128 channel EEG. The ${\gamma}$-band oscillatory activities were calculated by using time-frequency wavelet decomposition of the signal between 20 and 80Hz. The ${\gamma}$-band oscillatory activities of both groups were compared by t-test. Results : The ${\gamma}$-band oscillatory of the leads Fz, Cz, and Pz of both groups were represented well in the time-frequency maps. Significant increases of the ${\gamma}$-band activity in normal controls compared with schizophrenics were observed around 160 msec, 350 msec, and 800 msec after stimulation. Conclusions : Our results suggested that the increment in ${\gamma}$-band oscillatory activity during cognitive operations and decreased ${\gamma}$-band activity in schizophrenics may be associated with the cognitive dysfunctions and the pathophysiology of the schizophrenia.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.5
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• pp.1311-1321
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• 2010

Sensor network is used to obtain sensing data in various area. The interval to sense the events depends on the type of target application and the amounts of data generated by sensor nodes are not constant. Many applications exploit long sensing interval to enhance the life time of network but there are specific applications that requires very short interval to obtain fine-grained, high-precision sensing data. If the number of nodes in the network is increased and the interval to sense data is shortened, the amounts of generated data are greatly increased and this leads to increased amount of packets to transfer to the network. To transfer large amount of packets fast, it is necessary that the delay between successive packet transmissions should be minimized as possible. In this paper, we propose SET(SendDoneEventbasedTransmission Technique)which reduces the delay between successive packet transmissions by using SendDone Event which informs that a packet transmission has been completed. In SET, the delay between successive packet transmissions is shortened very much since the transmission of next packet starts at the time when the transmission of previous packet has completed, irrespective of the transmission time. Therefore SET could provide high packet transmission rate given large amount of packets.

• 한국체육학회지인문사회과학편
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• v.55 no.5
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• pp.447-457
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• 2016

The purpose of this study is to check awareness of 'the phenomenon of women's alienation in Yon-Ko-Jeon' to women students, and improve the gender equality for Yon-Ko-Jeon through IPA(Importance Performance Analysis). For this study, 322 women undergraduates at Yonsei University and Korea University, from sophomore to senior students, are selected and asked to be surveyed. At first, a majority of women students from two universities didn't recognize the lack of women sports and athletes in Yon-Ko-Jeon. Moreover, they didn't think about women's alienation in Yon-Ko-Jeon as well as improvement for the issue seriously. It shows the life style of gender in the sport. Second, as a result of IPA, improvement plan of Yon-Ko-Jeon on gender equality shows it needs to reflect member's opinion and introduce women sports clubs first. Recently, the improvement for the Yon-Ko-Joen is the top priority because the event doesn't reflect member's opinion. Furthermore, it shows that Yon-Ko-Jeon needs to introduce the competition of women's club sports. These are what the event has to improve.

• Korean Journal of Social Welfare Studies
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• v.41 no.3
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• pp.349-378
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• 2010

The Life-Course perspective is a theoretical orientation that views the life-course as the age structure embedded in social institutions and history and understands the life-course of individual and group in the contextual perspective. The life-course perspective studies were developed in Germany and North America. They study social and historical effects and the effect of institutions and the state on the life-course, the pathway of the work career and differences of labour outcomes, and the inequality that is developed in the life-course. In Korea, the life-course perspective studies were tried in various fields and cumulated. However, it didn't established as a theoretical orientation. For applying the life-course perspective to connect the individual life-course with social and historical event, I describe the historical location of individuals born between 1930 and 1979 and analyse the change of their transitions to adulthood exploratorily. On results, the extension of education made the structural change of their life-course, and in young cohort the timing of leaving school, entering workforce, marriage and childbirth was delayed and transitions were made in narrower spread. It means the standardization of the life-course as appears in modern society. The 1970s birth cohort has the differenciated life-course pattern, however I cannot verify that change because of the right censoring.

• Korean Journal of Child Studies
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• v.26 no.1
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• pp.329-345
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• 2005

Gender and intelligence of children, parenting practices and socioeconomic status of parents were studied to investigate whether they functioned as protective factors for resilient children. Total of 556 children of 12 years of age were contacted for the study. Teachers were asked to rate the children's interpersonal competence. Children reported their experience of stressful life events on a checklist. Based on the competence scores and the experience of stressful life events, 107 children were classified into adaptive, resilient, and maladaptive group. IQ scores were assessed using an intelligence test for the 107 children. Mothers reported their parenting practices and their socioeconomic status through a questionnaire. Descriptive statistics, chi-squares, t-tests, and one-way ANOVAs were performed to analyze the data. There was no difference in their experience of stressful life events between boys and girls. Girls were rated as being more competent by their teachers. There were more girls in the adaptive and the resilient group and more boys in the maladaptive group. Children in adaptive and the resilient group had higher intelligence scores than the maladaptive group. Mothers of resilient children replied as being warm and acceptive and more refusing and controlling than mothers of the adaptive children Group differences in socioeconomic status of the family were not found. Thus, being a girl and having higher IQ scores were the protective factors for the resilient children.