• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.221-225
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• 2005

A Staphylococcus sp. EY-3 with the capability of decolorizing Congo Red was isolated from soil at an effluent treatment plant of a textile and dyeing industry. This strain was able to almost completely decolorize a high concentration of Congo Red in 48 h under aerobic conditions. Optimal color removal (more than 96%) was achieved at 30- 40oC, and no noticeable effects of different pH values (5.5- 8.0) on decolorization were observed. This strain also exhibited a remarkable decolorization capability against azo dyes under aerobic conditions, even at a high concentration (dyes 1 g/l) of dye. The metabolic product of Congo Red degradation by this strain was identified by gas chromatography with mass selective detection (GC/MSD) to be an amine derivative benzidine.

• BMB Reports
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• v.46 no.12
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• pp.567-574
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• 2013

Liver plays a major role in maintaining glucose homeostasis in mammals. Under fasting conditions, hepatic glucose production is critical as a source of fuel to maintain the basic functions in other tissues, including skeletal muscle, red blood cells, and the brain. Fasting hormones glucagon and cortisol play major roles during the process, in part by activating the transcription of key enzyme genes in the gluconeogenesis such as phosphoenol pyruvate carboxykinase (PEPCK) and glucose 6 phosphatase catalytic subunit (G6Pase). Conversely, gluconeogenic transcription is repressed by pancreatic insulin under feeding conditions, which effectively inhibits transcriptional activator complexes by either promoting post-translational modifications or activating transcriptional inhibitors in the liver, resulting in the reduction of hepatic glucose output. The transcriptional regulatory machineries have been highlighted as targets for type 2 diabetes drugs to control glycemia, so understanding of the complex regulatory mechanisms for transcription circuits for hepatic gluconeogenesis is critical in the potential development of therapeutic tools for the treatment of this disease. In this review, the current understanding regarding the roles of two key transcriptional activators, CREB and FoxO1, in the regulation of hepatic gluconeogenic program is discussed.

• Journal of Microbiology
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• v.40 no.3
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• pp.245-247
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• 2002

A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp ［(A/T)TTTA(T/C)(A/G)TTT(A/T)］consensus sequence of the budding yeast ARS.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.173-176
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• 2002

Effect of carbon source and culture conditions involved in the concentration of dissolved oxygen on cell growth and the production of pullulan by A. pullulans HP2001 were investigated. Among those carbon sources, glucose was found to be the best carbon source for the production of pullulan by A. pullulans HP2001. Maximal production of pullulan by A. pullulans HP2001 was 26.6 g/ f when concentrations of glucose and yeast extract were 8% (w/v) and 0.25% (w/v), respectively. It was found that aeration rate, agitation speed and inner pressure of a bioreactor, which were some of physiological factors involved in the dissolved oxygen in the medium may affect cell growth and the production of pullulan by A. pullulans HP2001.

• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.53-58
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• 2002

Adverse environmental conditions such as drought, high salt and cold/freezing are major factors that reduces crop productivity worldwide. According to a survey, 50-80% of the maximum potential yield is lost by these "environmental or abiotic stresses", which is approximately ten times higher than the loss by biotic stresses. Thus, improving stress-tolerance of crop plants is an important way to improve agricultural productivity, In order to develop such stress-tolerant crop plants, we set out to identify key stress signaling components that can be used to develop commercially viable crop varieties with enhanced stress tolerance. Our primary focus so far has been on the identification of transcription factors that regulate stress responsive gene expression, especially those involved in ABA-mediated stress response. Be sessile, plants have the unique capability to adapt themselves to the abiotic stresses. This adaptive capability is largely dependent on the plant hormone abscisic acid (ABA), whose level increases under various stress conditions, triggering adaptive response. Central to the response is ABA-regulated gene expression, which ultimately leads to physiological changes at the whole plant level. Thus, once identified, it would be possible to enhance stress tolerance of crop plants by manipulating the expression of the factors that mediate ABA-dependent stress response. Here, we present our work on the isolation and functional characterization of the transcription factors.n factors.

• Journal of Surface Science and Engineering
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• v.49 no.5
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• pp.447-453
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• 2016

In this study, we intended to extend the life of electropolishing solution through the reduction of electric resistance by improving the electrolysis efficiency. The optimum conditions were obtained by half round bus bar and Taguchi method. As the main control factors in the electropolishing process, current density, polishing time, electrolyte temperature and flow rate were selected. The electrolyte temperature was the most significant to the electrolysis efficiency. The optimum conditions for the life extension of electropolishing solution were as follows: current density, $45A/dm^2$; polishing time, 6 min; electrolyte temperature, $70^{\circ}C$; flow rate, 11 L/min. As a results of ANOVA of SN ratios, it was found that the electrolyte temperature was significant factor at the 90% confidence level.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.3
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• pp.133-141
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• 2006

The Low cycle fatigue behavior of 11.7Cr-1.1Mo heat-resisting steel has been investigated under strain-controlled conditions with mean stresses at room temperature and $300^{\circ}C$. For the tensile mean stress test, the initial high tensile mean stress generally relaxed to zero at room temperature, however, at $300^{\circ}C$ initial tensile mean stress relaxed to compressive mean stress. Low cycle fatigue lives under mean stress conditions are usually correlated using modifications to the strain-life approach. Based on the fatigue test results from different stain ratio of -1, 0, 0.5, and 0.75 at room temperature and $300^{\circ}C$, the fatigue damage of the steel was represented by using cyclic strain energy density. Total strain energy density considering mean stress indicated well better than not considering mean stress at $300^{\circ}C$. Predicted fatigue life using Smith-Watson-Topper's parameter correlated fairly well with the experimental life at $300^{\circ}C$.

• Journal of Korea Society of Industrial Information Systems
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• v.12 no.3
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• pp.1-9
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• 2007

This paper deals with manufacturing conditions to increase the working die's life span which are the key factor in the manufacturing process of the coins and medals. Using the boundary element method, the stress diagram for the coining die was obtained, which was used to find the life span of working die. To iud the working die's life span related to the diameter of collar and blank coin, eight different sizes were tested and the optimal condition was obtained. With the adjusted impressing force, the working die's life span was increased up to 60 percent.

• Journal of the Korean Society of Safety
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• v.4 no.1
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• pp.25-39
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• 1989

Cyclic temperature creep tests were carried out an AS 7075 alloy specimens were subjected to a constant load and stepwise temperature cycles in which temperature was fluctuated between 30$0^{\circ}C$ and 25$0^{\circ}C$ with three different cycle ratios. The highest frequency of cycling was 1 cycle per 10 hr and the lowest one was 1 cycle per 12 hr. From the creep experimental results with the above conditions the creep strain under cyclic temperature can be predicted easily by introd ucing the equivalent steady temperature because defined by Eq.(16), but the rupture life is 1.1 time than those of constant temperature because of effect of temperature history at tertiary creep range. Besides thlis result, the results of the creep test under cyclic temperature conditions are respectively compayiea with calculated rupture lives using the life fraction law and Eq.(18). The agreement between the obseried rupture times and calculated ones is fairly good. So creep rupture lives can be respectively predicted using life fraction law and Eq.(18).

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.46 no.5
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• pp.51-60
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• 2014

To find a practical application approach of polyelectrolyte multilayering (PEM) on inorganic filler, we introduced PEM process without washing step and investigated the effect of shear condition on the washless PEM treatment of ground calcium carbonate (GCC). Washless multilayering on GCC was conducted under various shear conditions such as stirring, homogenization, and ultrasonication. Highly charged polyelectrolytes combination of polydiallyldimethylammonium chloride (PDADMAC) and poly sodium 4-styrene sulfonate (PSS) and low charged polyelectrolytes combination with cationic starch and anionic polyacrylamide (PAM) were compared. In the case of highly charged polyelectrolytes combination, shear conditions did not affect the zeta potential and the particle size of treated GCC. However, the modified GCC particles with low charged polyelectrolytes were more dispersed under higher shear condition while maintaining the zeta potential. In addition, GCC was successfully modified through laboratory inline washless polyelectrolyte multilayering system which consists of homogenizers and pumps.