• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.

• Journal of the Korean Society for Library and Information Science
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• v.32 no.4
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• pp.249-274
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• 1998

It is the analyses of replies showed n the questionnaire consisted of four kinds of matters to see level of knowledge among SELIS (SEoul Women's University Library and Information System) OPAC users of keyword/boolean search. The result of this analyses is : in SELIS search, users who prefer keyword search than any other, who satisfy work of retrieval by means of boolean operator, and who think it easier, show lusher level of knowledge than those who deny it in the questionnaire. Knowledges Presented in the survey are ; characteristics of keyword search, single or double keys, using boolean operator in keyword, knowledge of index, knowledge of stop list, uncontrolled term. keyword search technique, right truncation, correct application of boolean logic operator, and selecting major subject in keyword browsing. The above mentioned knowledges will work as important factors n keyword/boolean search, OPAC. For successful search it requires conceptional knowledge of information retrieval processing, or inquiry word transformation how to search required information, and semantic ability to get result questioned In the given system, when and how to apply the characteristics of the system, and scientific record for user's inquiry, or fundamental computer technology and syntax knowledge to make search word in detail. But so far now important knowledge considered as user's online index search, has been emphasized on knowledge of scientific record, and has been lag of semantic and conceptional knowledge. So, it is recommendable for online index user to train to concentrate semantic knowledge, syntax ability, and conceptional knowledge, rather than scientific technique too much.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.40 no.1
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• pp.20-27
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• 2003

In this paper, we implemented a content-based image retrieval system that user can choose a wanted query region of object and retrieve similar object from image database. Query image is induced to wavelet transformation after divided into hue components and gray components that hue features is extracted through color autocorrelogram and dispersion in hue components. Texture feature is extracted through autocorrelogram and GLCM in gray components also. Using features of two components, retrieval is processed to compare each similarity with database image. In here, weight value is applied to each similarity value. We make up for each defect by deriving features from two components beside one that elevations of recall and precision are verified in experiment results. Moreover, retrieval efficiency is improved by weight value. And various features of database images are indexed automatically in feature library that make possible to rapid image retrieval.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.05a
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• pp.257-260
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• 2002

This paper describes a design of cryptographic processor that implements the AES (Advanced Encryption Standard) block cipher algorithm“Rijndael”. To achieve high throughput rate, a sub-pipeline stage is inserted into the round transformation block, resulting that the second half of current round function and the first half of next round function are being simultaneously operated. For area-efficient and low-power implementation the round transformation block is designed to share the hardware resources in encryption and decryption. An efficient scheme for on-the-fly key scheduling, which supports the three master-key lengths of 128-b/192-b/256-b, is devised to generate round keys in the first sub-pipeline stage of each round processing. The cryptoprocessor designed in Verilog-HDL was verified using Xilinx FPGA board and test system. The core synthesized using 0.35-${\mu}{\textrm}{m}$ CMOS cell library consists of about 25,000 gates. Simulation results show that it has a throughput of about 520-Mbits/sec with 220-MHz clock frequency at 2.5-V supply.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.39 no.11
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• pp.33-43
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• 2002

This paper describes a design of cryptographic processor that implements the AES (Advanced Encryption Standard) block cipher algorithm "Rijndael". To achieve high throughput rate, a sub-pipeline stage is inserted into a round transformation block, resulting that two consecutive round functions are simultaneously operated. For area-efficient and low-power implementation, the round transformation block is designed to share the hardware resources for encryption and decryption. An efficient on-the-fly key scheduler is devised to supports the three master-key lengths of 128-b/192-b/256-b, and it generates round keys in the first sub-pipeline stage of each round processing. The Verilog-HDL model of the cryptoprocessor was verified using Xilinx FPGA board and test system. The core synthesized using 0.35-${\mu}m$ CMOS cell library consists of about 25,000 gates. Simulation results show that it has a throughput of about 520-Mbits/sec with 220-MHz clock frequency at 2.5-V supply.

• The KIPS Transactions:PartD
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• v.9D no.4
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• pp.619-628
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• 2002

Reusable software component is an ultimate goal for the software development. Component based development is focused on advanced concepts rather than passive manipulation or class library with source codes. However, the primary component construction in component based development lead to an additional development cost and effort for reconstructing the new software component within a component model. Java application provides several features based on component model. But, we only have an opportunity to develop the smallest reuse units or the restricted set of GUI components. It cannot contributed as a component and only used in the specific domain component with high cost and efforts. In this paper, we apply java component model to the existing java application and extract javabeans through extending the component scalability. We also discuss the algorithm for transformation mechanism from legacy class to javabeans with a partial of business logic.

• Journal of Korean Library and Information Science Society
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• v.20
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• pp.241-284
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• 1993

Through the restoration, we can find that the register of houses, inhabitants and their ancestry, which is in the Sunsung Kim family registers, was fundamentally the census register of Kim Roe including more ancestry transformed into the lists of house, inhabitants and their ancestry of Kim Roe, Kim Bang-Seek, Kim Sung-Sae and Kim Hee-Bo. And we can find also the original forms were considerably damaged. That is, in the course of transformation, considerable parts of the contents of ancestry were not needed, so they were removed, the recordings of brotherandsisters, sons and their servants were also removed. By comparing of the restored census register of Kim Roe and the Sunsung Kim family registers, we can know the fact that when they published the family registers, the contents of the founder to the ninth descendants were totally depended on the census register of Kim Roe. The Census Register of King Taejo of Choson Dynasty(National Treasure No. 131) which has been recorded almost same periods as the census register of Kim Roe was remained as an original state. Therefore, it was greatly helpful for restoring the census register of Kim Roe. There were few materials which we can know the way of ordinary life in Koryo period. But through the census register of Kim Roe and the census Register of King Taejo of Choson Dynasty, we have a glimpse of their life history. Nevertheless we can find some demerits in the census register of Kim Roe as followers : First, it is not an original but a transformed one, while the Census Register of King Taejo of Choson Dynasty is first materials. Second, it was recorded the only one family. Finally, it was omitted the parts of his brotherandsisters, children and servants who lived with their master. According to these demerits its worth of materials for history will be descended more or less. Therefore, when we use this material, we should treat it more considerably.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.26 no.1
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• pp.217-231
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• 2015

This study aims to infer the gene-protein 'brings_about' chains of pancreatic cancer which were referred to in the pancreatic cancer related researches by constructing the gene-protein interaction network of pancreatic cancer. The chains can help us uncover publicly unknown knowledge that would develop as empirical studies for investigating the cause of pancreatic cancer. In this study, we applied a novel approach that grafts text mining and the main path analysis into Swanson's ABC model for expanding intermediate concepts to multi-levels and extracting the most significant path. We carried out text mining analysis on the full texts of the pancreatic cancer research papers published during the last ten-year period and extracted the gene-protein entities and relations. The 'brings_about' network was established with bio relations represented by bio verbs. We also applied main path analysis to the network. We found the main direct 'brings_about' path of pancreatic cancer which includes 14 nodes and 13 arcs. 9 arcs were confirmed as the actual relations emerged on the related researches while the other 4 arcs were arisen in the network transformation process for main path analysis. We believe that our approach to combining text mining analysis with main path analysis can be a useful tool for inferring undiscovered knowledge in the situation where either a starting or an ending point is unknown.

• Journal of Plant Biotechnology
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• v.1 no.1
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• pp.46-55
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• 1999

The expressed sequence tags(ESTs) from immature seed of rice, Oryza sativa cv Milyang 23, were partially sequenced and analyzed by homology. As of 1998, the partial sequences of about 6,600 cDNA clones were analyzed from normal and normalized immature seed cDNA libraries. About 2,200 ESTs were putatively identified by BLASTX deduced amino acid sequence homology analysis. About 20% of them were putatively identified as storage proteins. Also the clones were highly homologous to genes involved particularly in starch biosynthesis, glycolysis, signal transduction and defenses. Compared to 35% of redundancy in the ESTs of normal cDNA library, that from the substracted library was 15%. The Korea Rice Genome Network is maintained to provide the updated information of sequences, their homologies and sequence alignments of ESTs. For the stable expression of transgene in rice, diverse vectors were developed for overexpression, targeting and gene dosage effect with transit peptides (Tp) and matrix attachment region (MAR) sequence from chicken lysozyme locus. The rice calli were transformed via Agrobacterium tumefaciens LBA4404(pSB1) with the triparental mating technique and selected by herbicide resistance. The green fluorescent protein(GFP) gene in expression vector under the control of rbcS promoter-Tp was overexpressed upto 10 % of the total soluble protein. In addition, the Tp-sGFP fusion protein was properly processed during translocation into chloroplast. The expression of sGFP in the presence of MAR sequences was analyzed with Northern and immunoblot analysis. All the lines in which sGFP transgene with MAR sequence, showed position independent and copy number-dependent expression, while the lines without MAR showed the varied level of expression with the integration site. Thus the MAR sequence significantly reduced the variation in transgene expression between independent transformants.

• Journal of the Korean Society for Library and Information Science
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• v.49 no.3
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• pp.371-392
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• 2015

This research attempted to provide new philosophical perspective to be taken in the public libraries as an institution which should support the social inclusion in the multicultural society. As deepening on dichotomous system of culture in modern society, It is referred to the need for an intercultural discourse on mulitcultural services in the public libraries. The process of social Integration suggested by Esser indicated the interactive transformation between actors and society. The conception serves the standard perspective on immigrants. Accordingly, This research analyzed a multicultural program of public libraries based on the classification of policy about immigrants by Castles. And it is based on the intercultural philosophy of Mall, It suggested the roles of public libraries in social integration and the process to support immigrants through the framework by Esser.