• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.58-62
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• 2006

Rapid assay of enzyme is a primary requirement for successful application of directed evolution technology. Halo generation on a turbid plate would be a method of choice for high throughput screening of enzymes in this context. Here we report a new approach to prepare turbid plates, by controlling the crystallization of tyrosine to form needle-like particles. In the presence of tyrosine phenol-lyase (TPL), the needle-like tyrosine crystals were converted to soluble phenol rapidly than the usual rectangular tyrosine crystals. When an error-prone PCR library of Citrobacter freundii TPL was spread on the turbid plate, approximately 10% of the colonies displayed recognizable halos after 24 hours of incubation at $37^{\circ}C$. Representative positives from the turbid plates were transferred to LB-medium in 96-wellplates, cultivated overnight, and assayed for the enzyme activity with L-tyrosine as the substrate. The assay results were approximated to be proportional to the halo size on turbid plates, suggesting the screening system is directly applicable to the directed evolution of TPL. Actually, two best mutants on the turbid plates were identified to be $2{\sim}2.5$ and 1.5-fold improved in the activity.

• Journal of Conservation Science
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• v.36 no.2
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• pp.73-81
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• 2020

Considering the difficulties associated with the creation of deterioration maps for stone cultural heritages, quantitative determination of chemical and biological contaminants in them is still challenging. Hyperspectral image analysis has been proposed to overcome this drawback. In this study, hyperspectral imaging was performed on Stone Buddhas Temple in Four Directions at Gulbulsa Temple Site(Treasure 121), and several surface contaminants were observed. Based on the color and shape, these chemical and biological contaminants were classified into ten categories. Additionally, a method for establishing each class as a reference image was suggested. Simultaneously, with the help of Spectral Angle Mapper algorithm, two classification methods were used to classify the surface contaminants. Method A focused on the region of interest, while method B involved the application of the spectral library prepared from the image. Comparison of the classified images with the reference image revealed that the accuracies and kappa coefficients of methods A and B were 52.07% and 63.61%, and 0.43 and 0.55, respectively. Additionally, misclassified pixels were distributed in the same contamination series.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.4
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• pp.43-54
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• 2007

This paper presents design and verification of a face detection hardware for real time application. Face detection algorithm detects rough face position based on already acquired feature parameter data. The hardware is composed of five main modules: Integral Image Calculator, Feature Coordinate Calculator, Feature Difference Calculator, Cascade Calculator, and Window Detection. It also includes on-chip Integral Image memory and Feature Parameter memory. The face detection hardware was verified by using S3C2440A CPU of Samsung Electronics, Virtex4LX100 FPGA of Xilinx, and a CCD Camera module. Our design uses 3,251 LUTs of Xilinx FPGA and takes about 1.96${\sim}$0.13 sec for face detection depending on sliding-window step size, when synthesized for Virtex4LX100 FPGA. When synthesized on Magnachip 0.25um ASIC library, it uses about 410,000 gates (Combinational area about 345,000 gates, Noncombinational area about 65,000 gates) and takes less than 0.5 sec for face realtime detection. This size and performance shows that it is adequate to use for embedded system applications. It has been fabricated as a real chip as a part of XF1201 chip and proven to work.

• The Journal of Korean Academy of Sensory Integration
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• v.12 no.2
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• pp.25-36
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• 2014

Objective : The purpose of this study is learning the characteristics of literature applying single subject researches in the field of sensory integration therapy of Korean occupational therapy and evaluating the quality level of them. Methods : Analyzed the characteristics of 17 single subject research papers (independent variable, study design, study subject, total number of session, study period, intervention time, intervention place, dependent variable, measuring tool and result) published between 2002 and 2013 using the document delivery service of Korean Society of Occupational Therapy, National Discovery for Science Leaders (NDSL), Nuri Media (DBpia), Research Information Sharing Service (RISS), Korean Studies Information Service System (KISS) and National Assembly Library and evaluated the literatures using quality evaluation scale. Results : According to the analysis result on the literature characteristics, reversal design was the most used study method. Total number of session was 10 sessions to 34 sessions. Study period was 4 weeks to 16 weeks. Intervention time was 8 minutes to 70 minutes and most interventions were done in occupational therapy room or sensory integration therapy room. According to the result of quality level evaluation, 4 papers out of 17 papers were on high level and remaining papers were on intermediate level. However, there was no study which included intervention blind. Conclusion : Suggested standards that need to be supplemented and discussions on qualitative improvement including repetitive research on the mediation effect during the application of the single subject research methodology, the securement of adequate data sections, and processing of a mediation blind. It is expected to be used as basic data for conducting a better qualitative research in the future.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.279-282
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• 2017

Kinect depth sensor, a depth camera developed by Microsoft as a natural user interface for game appeared as a very useful tool in computer vision field. In this paper, due to kinect's depth sensor and its high frame rate, we developed a distance measurement system using Kinect camera to test it for unmanned vehicles which need vision systems to perceive the surrounding environment like human do in order to detect objects in their path. Therefore, kinect depth sensor is used to detect objects in its field of view and enhance the distance measurement system from objects to the vision sensor. Detected object is identified in accuracy way to determine if it is a real object or a pixel nose to reduce the processing time by ignoring pixels which are not a part of a real object. Using depth segmentation techniques along with Open CV library for image processing, we can identify present objects within Kinect camera's field of view and measure the distance from them to the sensor. Tests show promising results that this system can be used as well for autonomous vehicles equipped with low-cost range sensor, Kinect camera, for further processing depending on the application type when they reach a certain distance far from detected objects.

• Journal of Digital Convergence
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• v.16 no.7
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• pp.35-45
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• 2018

Research on the establishment of new governance aimed at efficient policy planning and the implementation thereof by the government has been conducted in response to social changes. Nonetheless, governance is comprehensive and encompasses different meanings; it takes various forms in the process of its actual application. Therefore, systematic classification of research on governance and analysis on its research trend are required. Accordingly, three researchers who majored in policy sciences, business informatics, and library and information science, respectively, searched for theses related to governance published since 2016 from Research Information Sharing Service and conducted a social network analysis (SNA) on them. According to their research results, the main research topics were largely classified into collaborative governance and local governance. Keywords throughout the topics included network, participation, conflict, and trust in line with the characteristics of governance. Representative subjects of governance included education, urban regeneration, and the environment. Further, measurement of betweenness centrality showed local governance was a main topic for convergent research. This study will lead to a greater understanding of research on governance and help activate such research. One limitation of this study is that it analyzed only theses with the keywords but not all theses on governance. Follow-up research should analyze all theses on governance and statistically verify them with SNA indexes.

• Journal of Industrial Convergence
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• v.18 no.5
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• pp.42-53
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• 2020

This study approached the elderly in Korea with a systematic review to find out the effect of virtual reality program arbitration on balance, which the evidence for the virtual reality program is provided. Total of 94 papers were searched through the database Nuri Media (DBpia), Scholarship (earticle), Korean Studies Information (KISS), National Digital Science Library (NDSL), the Korea Educational Research and Information Service (RISS), Kyobo Book Scholar (RISS), and Hakjisa New Thesis on Literature Selection using PRISMA flow-chart from January 2005 to May 2020 based on the final literature selection process and analysis. The quality level of the literature was found to be three volumes (50.0%) of the base level I, one (16.7%) of the II, and two of the III (33.3%). The most common type of virtual reality program was Wii-fit balance of 4 (66.7%), and the effect of virtual reality program arbitration was significant overall through evaluation tools for balance and walking ability. This is expected to effectively apply the virtual reality program to the elderly. In addition, since clinical application basis has been provided, further studies applying various virtual reality program interventions need to be addressed.

• The KIPS Transactions:PartD
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• v.8D no.6
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• pp.705-714
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• 2001

The computing systems of today expanded business trade and distributed business process Internet. More and more systems are developed from components with exactly reusability, independency, and portability. Component based development is focused on advanced concepts rater than passive manipulation or source code in class library. The primary component construction in CBD. However, lead to an additional cost for reconstructing the new component with CBD model. It also difficult to serve component information with rapidly and exactly, which normalization model are not established, frequency user logging in Web caused overload. A lot of difficult issues and aspects of Component Based Development have to be investigated to develop good component-based products. There is no established normalization model which will guarantee a proper treatment of components. This paper elaborates on some of those aspects of web application to adapt user requirement with exactly and rapidly. Distributed components in this paper are used in the most tiny size on network and suggest the network-addressable interface based on business domain. We also discuss the internal and external specifications for grasping component internal and external relations of user requirements to be analyzed. The specifications are stored on Servlets after dividing the information between session and entity as an EJB (Enterprise JavaBeans) that are reusable unit size in business domain. The reusable units are used in business component through query to get business component. As a major contribution, we propose a systems model for registration, auto-arrange, search, test, and download component, which covers component reusability and component customization.

• The KIPS Transactions:PartD
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• v.13D no.3 s.106
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• pp.405-414
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• 2006

These days, business environments are rapidly change and companies are taking the pressure about a customer value enhance to the continuance from the market. Therefore companies propel various innovative and strategic initiatives for a customer value enhancement. Also Information Technology is rapidly becoming part of our everyday lives due to its fast development, the functions and responsibilities of CSC(Consolidated Service Center) are undergoing revolutionary change. The function of sales organization based on the manpower and human networks are gradually shrinking, and CSC is slowly establishing itself as the company's core parts as its function expands and its operations are in the spotlights. Among these, many organizations are currently introducing ITSM concept for primary processes as a means of achieving synergy in operational excellence, reducing costs and improving service quality. In this thesis, we propose an extended CSC, which is applied ITIL Process to traditional BMP. It also needs to manage more effectively business process model, to modeling rapidly changing business process, and to developing its business application programs whenever and whatever it is changed. The ITSM(IT Service Management), which is an ITIL tool, effectively managing for dynamically changing the business process.

• Journal of Energy Engineering
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• v.16 no.4
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• pp.155-163
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• 2007

To insure nuclear reactor safety, the reactivity of control rods should be calculated by measuring the criticality of reactor core and it is regularly performed during the annual physics test period. Also, the core criticality should be monitored during the start-up operation to avoid reactivity induced accidents. Many research works on control rod reactivity measurement and subcriticality measurement have been accomplished throughout the world for decades and recently a new method named "Modified Neutron Source Multiplication Method (MNSM)" was proposed in Japan which is known to be improved overcoming limitations of traditional Neutron Source Multiplication Method (NSM). In this study, MNSM was tested in calculation of subcriticalities and in evaluation of application validity using the educational reactor in Kyung Hee University, AGN-201. For this study, a revised nuclear data library and a neutron transport code system TRANSX - PARTISN were established. Correction factors for various control rod positions were produced using the k-effective values and the corresponding flux distributions and adjoint flux distributions. Experimental values of the core criticality were obtained using the neutron count rates of the BF3 proportional counters. The results showed that the expected reactivity worth of control rods by MNSM agreed well with the theoretical values and the correction factors contributed much for this purpose.