• 제목/요약/키워드: Level of enrichment

검색결과 225건 처리시간 0.025초

Tryptophan 및 Tyrosine 보강 식이가 Stress로 인한 혈청 Cortisol, Glucose 및 Free Fatty Acid의 농도 변화에 미치는 영향 (The Effects of Tryptophan and Tyrosine-Enriched Diet on the Serum Cortisol, Glucose and Free Fatty Acid Levels of Stressed Rats)

  • 최정희
    • Journal of Nutrition and Health
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    • 제23권4호
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    • pp.229-236
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    • 1990
  • Sprague-Dawley 숫쥐에게 3.6%의 tryptophan 또는 tyrosine 보강 식이를 4일간 먹여 스트레스로 인한 혈청 cortisol, glucose, free gatty acid(ffa)수준 변화를 조사하였다. Trp-보강 식이를 먹은 쥐들은 혈청 및 뇌의 trp농도가 유의적으로(p<0.05) 증가하였고 cortisol과 ffa 수준은 정상 식이군과 같았으나 glucose는 유의적으로 낮았다. Trp- 보강 식이군이 stress를 받았을 때 혈청 cortisol 수준은 유의적으로 증가하였으나 정상식이군의 stress 반응 정도에는 미치치 못하는 유의적으로 낮은 증가 추세를 보였다. 또한 trp-보강 식이군의 혈청 glucose와 ffa 수준은 정상식이군과는 달리 스트레스로 인한 증가 현상이 나타나지 않았다. 이로서 Trp-보강 식이는 스트레스로 인한 대사적 교란(metabolic disturbance)를 방지하고, 뇌하수체-시상하부-부신 피질계를 통한 호르몬 반응의 강도를 줄이는 효과를 나타내었다. 한편 try-보강 식이를 먹은 쥐들은 혈청 try함량이 증가하였고 유의적이지는 않으나 전반적으로 낮은 cortisol 수준을 보였다. 이들의 giucose와 ffa농도는 정상 식이군과 비교해 차이가 없었다. Try-보강 식이군이 스트레스를 받으면 혈청 cortisol 수준에는 유의적인 변화가 없는 반면 glucose와 ffa함량은 정상 식이군과 동일하게 증가하였다. 즉 try보강 식이는 스트레스로 인한 대사적 교란을 방지할 수는 없었으나 시상하부-뇌하수체-부신 피질게를 통해 이루어지는 hormone 반응을 말소시킬 수 있는 것으로 보여진다.

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Combination of Hydrophobic Filtration and Enrichment Methods for Detecting Bacillus cereus in Fresh-Cut Cabbage

  • Lee, Sujung;Choi, Yukyung;Lee, Heeyoung;Kim, Sejeong;Lee, Jeeyeon;Ha, Jimyeong;Oh, Hyemin;Lee, Yewon;Kim, Yujin;Yoon, Yohan;Lee, Soomin
    • 한국식품위생안전성학회지
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    • 제33권5호
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    • pp.325-329
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    • 2018
  • 본 연구에서는 신선편이 양배추 내 Bacillus cereus의 최적 증균 온도를 선정하고 증균배양액에 소수성필터를 적용하여 multiplex PCR의 검출률을 확인하였다. B. cereus 증균온도는 B. cereus 균주 5 개를 혼합하여 1 Log CFU/mL이 되도록 증균배지에 접종하고 $30^{\circ}C$, $37^{\circ}C$, $42^{\circ}C$에서 증균한 뒤 3시간 간격으로 MYP agar에 도말한 후 계수하여 선정하였다. 소수성필터 미적용 그룹은 B. cereus 균주 5 개를 혼합하여 신선편이 양배추에 접종한 뒤 최적 증균온도에서 증균하였으며, 증균배양액을 가열하여 DNA를 추출한 뒤 multiplex PCR을 진행하였다. 소수성필터 적용 그룹은 증균배양액을 소수성 필터에 적용하고 필터에 있는 균을 멸균증류수로 현탁한 뒤 가열하여 추출된 DNA로 multiplex PCR을 진행하였다. 증균온도 확인 결과, 6시간 증균 시 $42^{\circ}C$에서 증균된 샘플($5.4{\pm}0.3Log\;CFU/mL$)과 $30^{\circ}C$에서 증균된 샘플($4.6{\pm}0.6Log\;CFU/mL$) 간 유의차가 확인되었다(p < 0.05). 소수성필터 적용 유무에 따른 multiplex PCR 결과, 1 Log CFU/g 접종된 샘플의 검출률이 소수성 필터 적용 전 60%(3/5)에서 100%(5/5)로 향상되었다. 2 Log CFU/g 접종 샘플은 소수성필터 적용 전 80%(4/5)에서 소수성 필터 적용 후 100%(5/5)로 검출률이 증가하였으나, 3 Log CFU/g 접종 샘플은 소수성 필터 적용 전후 모두 100%(5/5)로 확인되었다. 이상의 결과를 통해 신선편이 양배추 내 B. cereus 검출 시 증균배양액에 소수성필터를 적용하고 multiplex PCR을 적용했을 때 신속하고 효율적인 검출이 가능할 것으로 판단된다.

의식주생활의 문화예술적 측면과 경험을 중심으로 한 가정과교사 직무연수 프로그램의 개발 및 적용 (Development and Application of a Home Economics Teacher Training Program Based on the Cultural & Artistic Aspects of Clothing, Food, and Housing Life and Experience)

  • 배현영
    • 한국의류학회지
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    • 제36권11호
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    • pp.1208-1221
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    • 2012
  • This study was conducted with the budgetary support of the Gyeonggi Provincial Government Department of Education as curriculum preparation (frequently revised since 2007) for the enhancement of teacher professionalism in Clothing, Food, and Housing Life education. This study promotes changes in student learning through the enrichment of Home Economics content and the classroom-learning environment through the reinforcement of teacher professionalism and attitudes towards Clothing, Food, and Housing Life education. This study enhances the comprehension of Home Economics and understanding by educators on the importance of Home Economics. The training program was evaluated through an analysis of the motive of application, level of satisfaction with the program, change in the view of Clothing Life education, and level of expectation and contributions of the program towards the curriculum development of teaching. The trainees were motivated by the opportunity to practice, uniqueness of the curriculum of the training program, and expectations for professional enhancement. The level of satisfaction is very high. Regardless of subjects, trainees recognized the necessity of practical exercise, cultural & artistic approach, and integrated teacher training in Clothing Life education. The teachers of other subjects recognized the importance of Home Economics and the historical background of Clothing, Food, and Housing Life.

Empowering Leadership and Individual Creativity: The Mediation Role of Psychological Empowerment in Facing Covid-19 Pandemic

  • SISWANTI, Yuni;MUAFI, Muafi
    • The Journal of Asian Finance, Economics and Business
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    • 제7권11호
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    • pp.809-816
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    • 2020
  • This study tests and analyzes the influence of empowering leadership on employee creativity which is mediated by psychological empowerment. Researchers conduct survey method on state-owned bank employees (ABC Bank) in Pacitan, East Java, Indonesia. Purposive sampling is selected with Likert scale as measurement. The population of this study is 166 respondents, who are permanent employees of the ABC Bank. The data analysis technique used is SPSS 24. The results of this study find that: (1) there is an influence of empowering leadership on psychological empowerment, (2) there is an influence of empowering leadership on individual creativity, (3) there is an influence of psychological empowerment on individual creativity, and (4) the influence of empowering leadership on individual creativity is mediated by psychological empowerment. This study ignores other types of leadership. Further study is needed to examine other types of leadership besides empowering leadership. The level of analysis of this study is individual, so further study could examine empowering leadership conceptualized at the group level because followers will be influenced not only by the dyadic relationship with the leader, but also by the dynamics of the members of the empowered group. This study provides theoretical enrichment from the aspect of empowering leadership, especially at the individual level. Leaders can increase employee creativity by empowering them to do their jobs. Additionally, by creating a work environment that ensures employees feel empowered psychologically.

RESRAD-RECYCLE 전산코드를 활용한 금속폐기물 내 우라늄 자체처분 허용농도 예비 평가 (Preliminary Evaluation of Clearance Level of Uranium in Metal Waste Using the RESRAD-RECYCLE Code)

  • 이선우;홍정환;박정석;김광표
    • 방사선산업학회지
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    • 제17권4호
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    • pp.457-469
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    • 2023
  • The clearance level by nuclide is announced by the Nuclear Safety and Security Commission. However, the clearance level of uranium existing in nature has not been announced, and research is needed. Therefore, the purpose of this study was to evaluate the clearance level of uranium nuclides appropriate to domestic conditions preliminary. For this purpose, this study selected major processes for recycling metal wastes and analyzed the exposure scenarios and major input factors by investigating the characteristics of each process. Then, the radiation dose to the general public and workers was evaluated according to the selected scenarios. Finally, the results of the radiation dose per unit radioactivity for each scenario were analyzed to derive the clearance level of uranium in metal waste. The results of the radiation dose assessment for both the general public and workers per unit radioactivity of uranium isotopes were shown to meet the allowable dose (individual dose of 10 µSv y-1 and collective dose of 1 Man-Sv y-1) regulated by the Nuclear Safety and Security Commission. The most conservative scenarios for volumetric and surface contamination were evaluated for the handling of the slag generated after the melting of the metal waste and the direct reuse of the contaminated metal waste into the building without further disposal. For each of these scenarios, the radioactivity concentration by uranium isotope was calculated, and the clearance level of uranium in metal waste was calculated through the radioactivity ratio by enrichment. The results of this study can be used as a basic data for defining the clearance level of uranium-contaminated radioactive waste.

보은지역 흑색셰일 분포지역에서의 암석-토양-식물계내 잠재적 독성원소들의 분산과 이동 (Dispersion and Migration of Potentially Toxic Elements in the Rock-Soil-Plant System from the Boeun Area Underlain by Black Shales, Korea)

  • 이진수;전효택;김경웅
    • 자원환경지질
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    • 제30권6호
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    • pp.587-601
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    • 1997
  • This study had three purposes: (1) to investigate the enrichment levels and dispersion patterns of potentially toxic elements in the rock-soil-plant system; (2) to evaluate the uptake ratios of heavy metals from soils into plants and (3) to assess the chemical speciation of heavy metals in soils. Rock, surface soil and plant samples were collected in the Boeun area underlain by black shales of the Okchon Zone. These samples were analyzed for multi-elements using INAA, ICP-AES and AAS. The maximum abundance of U in black shales is 16 mg/kg and radioactivity counts up to 300 cpm. In particular, Mo, V, Ba, Cd, Pb and U are enriched in black shales. Most of soils derived from black shales show high concentrations of U, As, Mo, Ba, Cu, Cd, Pb, Zn and mean concentrations of As and Mo in soils (20 mg/kg of As and 6.6 mg/kg of Mo) are higher than the permissible level suggested by Kloke (1979). Enrichment index values of soils are calculated and higher than 1.0 in the black shale area with the highest value of 6.4. Mean concentration of Cd in plants is higher than those of Cu, Pb and Zn. The concentration of Cd in plant species decreases in the order of Chinese cabbage > red pepper > soybean=sesame > rice stalk > com > rice grain. The biological absorption coefficients (BAC) in plants are in the order of Cd > Zn=Cu > Pb, which suggests that Cd is more bioavailable to plants than Cu, Pb and Zn. From the results of sequential extraction analysis of soils, relatively high proportion of Cu, Pb and Zn are present as residual fractions whereas that of Cd as non-residual fractions. Cadmuim occurs predominantly as exchangeable/water-acid soluble phase in soils, and Cd is more mobile and bioavailable than Cu, Pb and Zn.

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Modified sorbitol MacConkey agar for the rapid isolation of Escherichia coli O157:H7

  • Jung, Byeong-yeal;Jung, Suk-chan;Lee, Na-kyung;Cho, Seong-kun;Cho, Dong-hee;Her, Moon;Yoon, Yong-dhuk;Kim, Bong-hwan
    • 대한수의학회지
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    • 제39권4호
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    • pp.765-771
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    • 1999
  • Unlike most Escherichia coli strains, E coli O157 : H7 didn't ferment sorbitol within 24h of incubation and showed a negative reaction for $\beta$-glucuronidase. We developed a new medium for the rapid isolation of E coli O157 : H7 using sorbitol MacConkey agar with cefixime, potassium tellurite and 4-methylumbelliferyl-${\beta}$-D-glucuronide (MUG) as a primary plating medium. The addition of $20{\mu}g/ml$ of vancomycin in enrichment broth for E coli O157 : H7 inhibited lots of Gram positive bacteria. Three strains (10.3%) of 29 non-O157 E coli strains and 3 strains (8.3%) of 36 Salmonella spp were inhibited at the $0.05{\mu}g/ml$ of cefixime and 23 strains (79.3%) of 29 non-O157 E coli strains and 12 strains (33.3%) of 36 Salmonella spp were inhibited at the $2.0{\mu}g/ml$ of potassium tellurite. But none of the E coli O157 : H7 was affected at these concentration. The addition of MUG at $100{\mu}g/ml$ level to sorbitol MacConkey agar with cefixime and potassium tellurite (CTM-SMAC) aided in the rapid isolation of E coli O157 : H7 from samples by checking sorbitol-negative and $\beta$-glucuronidase negative phenotypes simultaneously. In conclusion, inoculation of a positive in the O157 screening test from enrichment broth on CTM-SMAC appeared to be a rapid, cost-effective and sensitive method for the isolation of E coli O157 : H7.

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곡류제품의 철 강화 시나리오 작성 및 평가 (Estimated Iron Intakes from Simulated Fortification to Selected Grain Products in the Korea Food Supply)

  • 정해랑;홍민지;;오세영
    • 대한지역사회영양학회지
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    • 제11권6호
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    • pp.808-813
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    • 2006
  • Until now, South Korea does not have either fortification or enrichment program as intervention tools although the addition of micronutrients to foods is for the most part not regulated. The aim of this study was to determine which scenario would most effectively reduce the proportion of the population with low iron intake while not putting other population groups at risk of excessive intakes. In order to investigate potential dietary consequences of iron fortification we analyzed 2 day dietary record data (n=3,955) from the 2001 National Nutrition Surveys. The Proportion of the population consuming dietary iron less than the estimated average requirement (EAR) ranged from $12.4{\sim}87.5%$ depending upon gender and age group. Iron fortification at the level of 100% of Recommended Intake (RI) per 100g to breads and instant o. dried noodles was estimated to result in a 15% decrease of proportion of those with iron less than EAR, while putting 1.4 % of the population greater than the Upper Limit (UL). Iron fortification appeared to be the most effective for the $15{\sim}19$ year old age group, showing 39% reduction of iron intake insufficiency. The results suggest that carefully designed fortification or enrichment to staple foods may contribute to increase dietary iron intakes of Koreans, especially for the young population with a high prevalence of iron inadequacy. As the estimation in this study was based solely upon dietary intake data, iron intake from supplements should be considered in further studies.

Interaction effects of pen environment and sex on behavior, skin lesions and physiology of Windsnyer pigs

  • Mkwanazi, Mbusiseni Vusumuzi;Kanengoni, Arnold Tapera;Chimonyo, Michael
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권3호
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    • pp.452-458
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    • 2019
  • Objective: The study was carried to determine the interaction effects of pen enrichment and sex on behavioral activities, skin lesions and physiology of Windsnyer pigs. Methods: Forty-eight growing Windsnyer pigs of both sex, with an average initial body weight of 21.6 (${\pm}9.01$) kg were used. Four pigs were randomly assigned to either enriched or barren pens at a stocking density of $0.35m^2/pig$. Enriched pens contained 2 L bottles filled with stones and suspended at head level on ropes stretching across the pens. In addition, two plastic balls (90 mm in diameter) and 500 mL bottles (235 mm long) were placed on the floor of each enriched pen. Results: Pigs in barren environments had higher heart rates (p<0.001) than those in enriched pens. There was an interaction of pen environment and sex on rectal temperature (p<0.001). Females in enriched pens had higher rectal temperatures (p<0.05) than females in barren pens. There was no interaction of pen environment and sex on time spent eating and drinking (p>0.05). Time spent bullying was influenced (p<0.05) by pen environment and sex. Female pigs in barren environment spent more time on bullying than females in enriched pens. There was an interaction of pen environment and sex on time spent lying down and walking (p<0.05). Female pigs in enriched pens spent more time lying down than females in barren pens. Males in barren pens spent more time walking than males in enriched pens while no effect of pen environment was observed in females. There was an interaction of pen environment and sex on the number of skin lesions in the head, neck and shoulder region and other parts of the body (p<0.05). Conclusion: It was concluded that pen enrichment reduced the number of skin lesions and anti-social behaviors, especially for female pigs. There is a need, therefore of housing indigenous pigs under confinement.

토지이용실태에 따른 하남산업단지 토양오염물질 분포 특성에 관한 연구 (Distribution Characteristics of Soil Contaminants in Hanam Industrial Complex, Gwangju by land use)

  • 공화진;위환;김승호;박옥현;장길식;정희윤;배석진;정숙경;조영관
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제23권2호
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    • pp.30-39
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    • 2018
  • Soil contamination survey was conducted during March - July, 2017 to obtain soil contamination profile of 16 organic and inorganic contaminants in Hanam industrial complex located in Gwangju, Korea. The concentrations of all surveyed contaminants except Cd showed were within 0.3~1.5 times of their natural background levels. Cd showed concentrations as high as 6.9 times of the background level, signifying the influence of the metal processing facilities in the complex. The concentrations of Zn, Pb and Hg in areas nearby industrial facilities were 1.3~5.5 times higher than those within the facility and green area. The concentration of Cu in the green area was 1.4~2.9 times higher than in other areas. The Soil Pollution Index (SPI) analysis revealed 54% of the total area belong to first-grade soil, 43% to second-grade, and 3% to third-grade. The Enrichment Factor (EF) of Zn, Pb, and Cd were 9.2, 15.6, and 88.5, respectively, indicating high accumulation and contamination of the soil with Cd.