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Antioxidative and Antimicrobial Activities of Lilium Species Extracts Prepared from Different Aerial Parts (부위별 식용백합 추출물의 항산화 및 향균효과)

  • Joung, Yong-Myeon;Park, Soo-Jin;Lee, Ki-Young;Lee, Ji-Yong;Suh, Jeung-Keun;Hwang, Seong-Yun;Park, Kyoung-Eun;Kang, Myung-Hwa
    • Korean Journal of Food Science and Technology
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    • v.39 no.4
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    • pp.452-457
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    • 2007
  • In this study, Lilium sp. were separated into bulbs, leaves, and flowers. Then, total polyphenol contents, electron donating ability (EDA), superoxide dismutase (SOD)-like activity, hydroxyl radical scavenging activity, and antimicrobial activity were measured from the extracts of each of the three aforementioned parts. The examination of physiologically active substances in the three parts revealed that Lilium davidii leaves had high total polyphenol contents, SOD-like activity, hydroxyl radical scavenging activity, and EDA, while the flowers of L. lancifolium showed high SOD-like activity, hydroxyl radical scavenging activity, and EDA, as well as a high level of total polyphenols in the bulb. Measurements of the antimicrobial activities of the extracts against Gram positive bacteria revealed that the leaves and flowers of L. davidii and L. lancifolium caused Bacillus subtilis and Salmonella enteritidis to form clear zones greater than 10 mm. Furthermore, the flowers of L. lancifolium showed particularly high antimicrobial activity against B. subtilis, and the flowers of L. davidii had high activity against S. enteritidis. For the Gram negative bacteria, the leaves and flowers of L. davidii and L. lancifolium caused Listeria monocytogenes and Escherichia coli to form clear zones greater than 10 mm, and finally, the flowers of L. davidii and L. lancifolium showed high antibacterial activity, with inhibition exceeding 12 mm.

Changes in physicochemical components of Astragalus membranaceus fermented with Phellinus linteus (상황버섯균 접종 황기의 배양 중 이화학적 성분변화)

  • Jang, Yeon-Jeong;Kim, Eun-Ju;Kim, So Young;Lee, Yun Hye;Park, Shin-Young
    • Food Science and Preservation
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    • v.23 no.5
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    • pp.680-688
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    • 2016
  • This study analyzed the changes in physicochemical components of Astragalus membranaceus (AM) fermented with Phellinus linteus. Moisture content, pH, total acidity, total reducing sugar content, extraction yield, free sugar content, free amino acid and isoflavonoid (calycosin, formononetin) were investigated. The moisture content was increased during fermentation with Phellinus linteus. The pH level increased while the total acidity significantly decreased during fermentation. The reducing sugar content were in the range of 0.32~0.61%. The extraction yield using water was higher than that using 80% ethanol. The major free sugars were identified as glucose, fructose, sucrose and the content of free sugars decreased through fermentation. However, the glucose and sucrose contents of the water extracts were increased. In addition, the free amino acid increased significantly during fermentation. Finally, calycosin and formononetins contents in water extracts of after 30 days of AM fermentaion with Phellinus linteus were (3.91 mg/100 g) and (1.38 mg/100 g), respectively. These results suggest that fermentation with Phellinus linteus could be used to increase the bioactivity of AM. The mycelium-fermented AM could be a valuable source of functional material and edible resource for industry.

Economic Effects of Eliminating Trade Barriers under Imperfect Competition (불완전경쟁하(不完全競爭下)에서의 무역장벽(貿易障壁) 완화효과(緩和效果))

  • Lee, Hong-gue
    • KDI Journal of Economic Policy
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    • v.14 no.2
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    • pp.29-54
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    • 1992
  • Recent studies on the economic effects of trade liberalization and economic integration have emphasized the significant gains associated with product differentiation and scale economies. Securing access to markets in other countries will make it possible to increase product variety and capture scale economies, thus, expanding the gains from trade. Liberalization is also expected to introduce foreign competition into the previously closed market. Concurrently, the liberalization will improve the competitive market environment for firms selling in the domestic market. Firms will be pressed to either exit or reduce cost. The output per firm, then, will increase due to the exit of rival firms, and the average total cost will decline due to the economies of scale. 'Rationalization' of the production process will eventually follow. This paper addresses the economic effects of (counterfactual) bilateral tariff elimination between Korea and Japan. It computationally assesses the gains from liberalization as well as the resource allocations and welfare effects associated with the tariff reduction. The endogenous determination of the key parameters distinguishes this paper from others. The firm's perceived elasticity of demand and elasticity of substitution in the present model are calibrated to be consistent with the base year data. Korea, Japan, and the rest of the world are modeled explicitly. The sectoral coverage of the model includes twenty-three tradable product categories based on three-digit SITC industries and seven nontradable categories based on one-digit SITC industries. Product categories are also classified into perfectly competitive and imperfectly competitive ones. In the imperfectly competitive industries, product differentiation exists at the firm level, while the perfectly competitive industries are characterized by national product differentiation. The simulation results of bilateral tariff reduction are reported. Tariff elimination tends to increase intra-industry trade flows so that the total amount of exports and imports of both countries expand. Yet, Japan is expected to increase the bilateral trade surplus in the wake of the mutual tariff reduction. Terms-of-trade for Korea will not change, while for Japan it will deteriorate. Equivalent variations reflecting the change in consumer surplus (welfare) will favor Korean consumers. Total output, however, will not change substantially, recording 0.5 and 0.6% for Japan and Korea, respectively. An interesting finding in the analysis is that the gains from increased competition and scale efficiency are not as prevailing as expected in theory.

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Effects of Dietary Supplementation of Coffee Meal on Intestinal Enzyme Activity, Biochemical Profiles and Microbial Population in Broiler Chicks (커피박 첨가가 육계의 소장 효소 활성도, 생화학 지표 및 장내 미생물 균총에 미치는 영향)

  • Ko, Young-Hyun;Yun, Seo-Hyun;Song, Min-Hae;Kim, Se-Yun;Kim, Jong-Sun;Kim, Hyoun-Wook;Jang, In-Surk
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.105-113
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    • 2014
  • The current study was performed to investigate the effects of dietary supplementation of dried coffee meal (CM) on growth performance, intestinal and blood biochemical index, intestinal enzymes, and cecal microbial populations. A total of 162, 3-day-old male broiler chicks were randomly allocated into three dietary groups: control group (CON), basal diet added with 0.5% CM (CM I), and basal diet added with 1.0% CM (CM II). Dietary supplementation of CM did not change bird performance and the relative weight of intestinal mucosal tissues. The birds fed the diet supplemented with CM (0.5 and 1.0%) significantly decreased mucosal glucose concentration (P<0.05) without affecting blood glucose level compared with those fed control diet. The level of blood aspartate aminotransferase (AST) significantly increased in CM II group (P<0.05) without affecting ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GTP) compared with that in the CON group. The specific activity of intestinal maltase, leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) were not affected by dietary supplementation of CM, whereas sucrase activity in birds fed the diet supplemented with CM was decreased (P<0.05) compared to that in the control birds. The colony forming units (CFU) of E. coli in the cecum of CM-fed birds was significantly decreased (P<0.05) compared with that of control birds without changing the CFU of Lactobacillus. In conclusion, dietary supplementation of lower level of CM (0.5%) can be used as a beneficial feed resource without liver toxicity in broiler chicks.

Antioxidant Effects and Tyrosinase Inhibition Activity of Oriental Melon (Cucumis melo L. var makuwa Makino) Extracts. (참외 추출물의 항산화 효과 및 tyrosinase 저해활성)

  • Shin, Yong-Seub;Lee, Ji-Eun;Yeon, Il-Kweon;Do, Han-Woo;Cheung, Jong-Do;Kang, Chan-Ku;Choi, Seng-Yong;Youn, Sun-Joo;Cho, Jun-Gu;Kwoen, Dae-Jun
    • Journal of Life Science
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    • v.18 no.7
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    • pp.963-967
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    • 2008
  • The biological activities of water extracts from different fruit parts, such as peel, flesh, and placenta, of oriental melon were investigated. The concentrations of total phenolic in fruit extracts were $816.37\;{\mu}g/ml$, $385.23\;{\mu}g/ml$, and $925.56\;{\mu}g/ml$, respectively. Whereas the total flavonoid content in the peel extracts was $231.21\;{\mu}g/ml$, those in the extract of flesh and placenta were $8.16\;{\mu}g/ml$ and $36.07\;{\mu}g/ml$, respectively. The DPPH free radical scavenging activity of each fruit extract at 10,000 ppm was 34.84% for peel, 10.70% for placenta and 9.26% flesh. The ABTS radical cation decolorizing activity of each fruit extract at 10,000 ppm was in fruit extracts were 72.92% for peel, 48.0% for flesh and 74.31% for placenta. In addition, xanthine oxidase inhibitory activity, ${\alpha}-Glucosidase$ inhibition activity, and tyrosinase inhibition activity of the peel extracts appeared to be higher than those of placenta and flesh. Taken together, these results indicated that the peel part of oriental melon contained higher level of total flavonoid content, and several physiological activities including antioxidation, ${\alpha}-Glucosidase$ inhibition activity, and tyrosinase inhibition activity than did the flesh and placenta parts, and suggested that the peel might have a potential to be applicable as a source for functional foods.

Anti-inflammatory Effect of Lactuca sativa L. Extract in Human Umbilical Vein Endothelial Cells and Improvement of Lipid Levels in Mice Fed a High-fat Diet (상추 추출물(Lactuca sativa L.)의 혈관내피세포에서 항염증 작용과 고지방 식이 생쥐에서 혈중 지질농도 개선에 미치는 영향)

  • Hwang-Bo, Jeon;Jang, Kyung Ok;Chung, Hayoung;Park, Jong-Hwa;Lee, Tae Hoon;Kim, Jiyoung;Chung, In Sik
    • The Korean Journal of Food And Nutrition
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    • v.29 no.6
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    • pp.998-1007
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    • 2016
  • The objective of this study was to investigate the effects of a lettuce (Lactuca sativa L.) extract on the inflammation of human umbilical vein endothelial cell (HUVEC) and blood lipid improvement in hypercholesterolemic mice fed a high cholesterol diet. The lettuce extract (100% ethanol extract) inhibited the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in HUVEC treated with tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$). The lettuce extract suppressed the adhesion of THP-1 to TNF-${\alpha}$-treated HUVEC. The lettuce extract decreased the TNF-${\alpha}$-stimulated production of proinflammatory cytokine interleukin-6, interleukin-8 and chemokine monocyte chemotactic protein 1. In hypercholesterolemic mice, the lettuce extract reduced serum total cholesterol, triglyceride, and low-density lipoprotein-cholesterol level, while the lettuce extract elevated high-density lipoprotein-cholesterol level, resulting in the decrease of atherogenic index and cardiac risk factor level. These results suggested that lettuce extract can be an useful resource to show an anti-inflammatory effect and improve lipid metabolism.

AICAR (5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside) Decreases Protein Synthesis in C2C12 Myotubes Cultured in High Glucose Media (근육세포 내 Glucose 농도와 AICAR에 의한 단백질 합성 저해)

  • Park, Chang-Seok;Kim, Jae-Hwan;Oh, Young-Kyoon;Kim, Kyoung-Hoon;Choi, Chang-Weon;Cho, Eun-Seok;Jeong, Yong-Dae;Park, Sung-Kwon
    • Journal of Animal Science and Technology
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    • v.54 no.5
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    • pp.369-373
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    • 2012
  • AMP-activated protein kinase (AMPK) maintains energy homeostasis in skeletal muscle. Nonetheless, its functional role on protein synthesis with different nutrient availability has not been elucidated. Therefore, the purpose of this study is to examine the effect of AMPK activity on protein content in C2C12 myotubes incubated with low (5 mM; LG) or high (25 mM; HG) glucose media. LG stimulated (p<0.05) AMPK and acetyl CoA carboxylase (ACC) activity compare to those in HG group. Total protein content was higher in myotubes cultured with HG than in those cultured with LG and further increased by AICAR (5-amino-1-${\beta}$-D-ribofuranosyl-imidazole-4-carboxamide). Myotubes cultured with HG showed 7.5% lower UbFL (Ubiquitin Firefly Luciferase)-to-SV40 (Simian virus40) ratio compared to those in LG. Glucose level did not change the phosphorylation level of mammalian target of rapamycin (mTOR). Interestingly, administration of AICAR significantly increased phosphorylation level of mTOR in myotubes cultured with LG but not in those with HG. Overall, this data indicate that AMPK activity and protein turnover are finely regulated in response to different glucose concentration.

Effect of Feeding Aspergillus oryzae Inoculant Food-waste Diets on Performance, $NH_3$ Emission and Fecal Microflora in Broiler Chickens (Aspergilius Oryzae 접종 남은 음식물 사료가 육계의 생산성, $NH_3$ 발생량 및 분내 미생물 성상에 미치는 영향)

  • Hwangbo J.;Hong E. C.;Lee B. S.;Bae H. D.;Kim W.;Nho W. G.;Kim J. H.;Kim I. H.
    • Korean Journal of Poultry Science
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    • v.32 no.4
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    • pp.281-289
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    • 2005
  • This study investigated the effect of dried food-waste diets(FW) fermented by Aspergillus oryzae(AO), on broiler growth performance, $NH_3$, emission and fecal microflora. Three hundreds broilers, two week old Hubbard strain, were randomly allotted to 4 experiments and fed with standards early boiler diet replaced with FW and AFW. In experiment 1, eighty four broilers were distributed into 7 treatments with 4 pens at 3 birds per replicate(pen). The dietary treatments ; T1 was com-soy bean meal based broiler diet(Control), T2, T3, T4 were for basal diet replaced with dried food waste without AO(FW) at the level of 20, 40 and $60\%$, respectively and T5, T6 and T7 followed the same levels for the basal diet but using Aspergillus oryzae inoculate food-waste(AFW). For experiments 2, 3, 4, seventy two broilers were distributed into 6 treatments with 4 pens at 3 birds per replicate(pen), respectively. The dietary treatments were the com-soy bean meal based broiler diet replacement with different combinations of FW and AFW, 1:0, 3:1, 1:1, 1:3, 0:1. at level of 20, 40 and $60\%$, respectively. In Exp. 1, it tended to be decreased in weight gain, however, there were no statistical differences among treatments except FW $60\%$ level of replacement(p<0.05). Feed intake and feed efficiency was not different among treatments. Total bacterial counts were not different between the control and FW diet, but E. coli decreased as the AFW levels of replacement were increased(p<0.05). There were no differences in weight gain, feed intake and feed efficiency among treatments in Exp. 2 and weight gains were lower fur FW diet compared with the control and AFW diet in Exp. 3(p<0.05). In Exp. 4, there were no differences in feed intakes among treatments, but lower in weight gain and feed efficiency in FW diet than that the control. In experiment 3, the $NH_3$ emission was the highest among treatments in FW/AFW 1:0 diet(p<0.05). From these results, it seems that FW would be supplemented up to $20\%$ in broiler diets and AO culture extract could improve FW value as feed supplements.

Relationships between Community Unit and Environment Factor in Forest Vegetation of Mt. Dutasan, Pyeongchang-gun (평창 두타산 산림식생의 군집유형과 입지환경요인의 상관관계)

  • Lee, Jeong Eun;Shin, Jae Kwon;Kim, Dong Gap;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.106 no.3
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    • pp.275-287
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    • 2017
  • The purpose of this study was to analyze forest vegetation type classification and relationships between the type and environment factor in Mt. Dutasan. Data were collected by total of forty six plots using Z-M phytosociological method from June to October, 2016, and analyzed by vegetation classification, canopy layer structure and relationships between vegetation unit and environment factor using coincidence methods. As a result of vegetation type classification, Quercus mongolica community group was classified at a top level of vegetation hierarchy that was classified into Rhododendron schlippenbachii community and Betula costata community. R. schlippenbachii community was divided into Lychnis cognata group and R. schlippenbachii typical group. L. cognata group was subdivided into Veratrum oxysepalum subgroup and L. cognata typical subgroup. B. costata community was divided into Fraxinus mandshurica group and Betula schmidtii group. F. mandshurica group was subdivided into Weigela subsessilis subgroup and Cimicifuga heracleifolia subgroup. Therefore the forest vegetation was composed of six vegetation units with two kinds of bisected species groups and fourteen species groups. As the result of an analysis of canopy layer structure, there were two kinds of structures with monotonous structures V. oxysepalum subgroup (vegetation units 1), L. cognata typical subgroup (vegetation units 2), W. subsessilis subgroup (vegetation units 4) and complicated structures R. schlippenbachii typical group (vegetation units 3), C. heracleifolia subgroup (vegetation units 5), Betula schmidtii group (vegetation units 6). The vertical layer structure of vegetation unit 5 was the most developed and vegetation unit 6 had the lowest coverage of herb layer. According to the correlation between vegetation unit and environmental factor, R. schlippenbachii community (vegetation units 1~3) and B. costata community (vegetation units 4~6) were classified based on 1,100 m of altitude, middle slope, twenty of slope degree, twenty percents of bare rock and thirty centimeters of DBH in tree layer. R. schlippenbachii community (vegetation units 1~3) showed positive correlation with altitude, topography and B. costata community (vegetation units 4~6) showed negative correlation tendency with them.

An Economic Factor Analysis of Air Pollutants Emission Using Index Decomposition Methods (대기오염 배출량 변화의 경제적 요인 분해)

  • Park, Dae Moon;Kim, Ki Heung
    • Environmental and Resource Economics Review
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    • v.14 no.1
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    • pp.167-199
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    • 2005
  • The following policy implications can be drawn from this study: 1) The Air Pollution Emission Amount Report published by the Ministry of Environment since 1991 classifies industries into 4 sectors, i. e., heating, manufacturing, transportation and power generation. Currently, the usability of report is very low and extra efforts should be given to refine the current statistics and to improve the industrial classification. 2) Big pollution industries are as follows - s7, s17 and s20. The current air pollution control policy for these sectors compared to other sectors are found to be inefficient. This finding should be noted in the implementation of future air pollution policy. 3) s10 and s17 are found to be a big polluting industrial sector and its pollution reduction effect is also significant. 4) The effect of emission coefficient (${\Delta}f$) has the biggest impact on the reduction of emission amount change and the effect of economic growth coefficient (${\Delta}y$) has the biggest impact on the increase of emission volume. The effect of production technology factor (${\Delta}D$) and the effect of the change of the final demand structure (${\Delta}u$) are insignificant in terms of the change of emission volume. 5) Further studies on emission estimation techniques on each industry sector and the economic analysis are required to promote effective enforcement of the total volume control system of air pollutants, the differential management of pollution causing industrial sectors and the integration of environment and economy. 6) Korea's economic growth in 1990 is not pollution-driven in terms of the Barry Commoner's hypothesis, even though the overall industrial structure and the demand structure are not environmentally friendly. It indicates that environmental policies for the improvement of air quality depend mainly on the government initiatives and systematic national level consideration of industrial structures and the development of green technologies are not fully incorporated.

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