• Title/Summary/Keyword: Lepus coreanus

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Development and characterization of nine microsatellite loci from the Korean hare (Lepus coreanus) and genetic diversity in South Korea

  • Kim, Sang-In;An, Jung-Hwa;Choi, Sung-Kyoung;Lee, Yun-Sun;Park, Han-Chan;Kimura, Junpei;Kim, Kyung-Seok;Min, Mi-Sook;Lee, Hang
    • Animal cells and systems
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    • v.16 no.3
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    • pp.230-236
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    • 2012
  • The Korean hare, Lepus coreanus, is an important mammal in ecosystem food chains, and is distributed across the entire Korean peninsula and northeastern China. Polymorphic microsatellite loci were developed using the biotinenrichment technique for use in population genetics studies. Five trinucleotide and four dinucleotide microsatellite loci were selected and tested on 22 Korean hare specimens collected from Gangwon Province and Gyeongsangbuk Province in South Korea. The number of alleles across the two sampling regions ranged from three to nine with a mean of 6.1. Mean observed and expected heterozygosities and polymorphic information content were 0.540, 0.627 and 0.579, respectively. Only one locus, Lc06, showed departure from Hardy-Weinberg equilibrium after applying the Bonferroni correction. Four microsatellites, Lc01, Lc03, Lc12, and Lc19, satisfied the criteria to serve as a core set of markers recommended for population genetics studies. These new microsatellite markers will be widely applicable to future genetic studies for management and conservation of the Korean hare and related species, including assessment of the genetic diversity and population structure of L. coreanus.

Molecular Sex Determination Using Sexual Dimorphisms between ZFX and ZFY Genes in Korean Hares(Lepus coreanus Thomas) (한국멧토끼 ZFX와 ZFY 유전자의 성별 이형성과 분자 성판별)

  • Han, Sang-Hyun;Cho, In-Cheol;Lee, Sung-Soo;Oh, Moon-You;Oh, Hong-Shik
    • Journal of Life Science
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    • v.17 no.3 s.83
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    • pp.402-406
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    • 2007
  • This study was performed to develop the molecular marker for sex determination of hare (Lepus coreanus) distributed in Korea which focused on sexual dimorphism between X and Y chromosomal homologous genes, zinc finger-X (ZFX) and -Y (ZFY). The intron 7 regions of ZFX and ZFY genes exhibited differential amplification patterns between male and female hares. The lengths of intron 7 region of ZFX and ZFY genes were 538 and 233-bp, respectively. Especially, the ZFX intron 7 contained a repetitive sequence identified as member of RNA-mediated transposable elements which was similar to CSINE2 commonly found in the rabbit genome. However, it was not present in intron 7 of ZFY gene. The molecular sex typing by polymerase chain reaction (PCR) was also carried out to determine the sex of hare based on difference in lengths between the intron 7 regions of ZFX and ZFY genes. All DNA samples tested had common band amplified from ZFX. However, the male hare DNAs had two distinct bands which amplified from ZFX and ZFY genes, respectively. The results from ZFX-ZFY PCR sex typing were identical to those from phenotypic investigation and from amplification patterns using male-specific sex determining region Y (SRY) gene as well. Finally, this study suggested that the sexual dimorphism between intron 7 regions of ZFX and ZFY could be useful genetic marker to determine sex of hare.

Monitoring and restoration plan after construction of Wildlife Corridor at Mt. Galmachi of Sungnam City (성남시 갈마치 지역의 야생동물이동통로 복원계획 및 조성 후 모니터링)

  • Chun, Seung-Hoon;Lee, Sang-Don;Chae, Soo-Kwon
    • Journal of Environmental Impact Assessment
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    • v.20 no.6
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    • pp.907-914
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    • 2011
  • This study aims at monitoring the efficiency of wildlife corridor in the city of Sungnam, Gyunggi Province. During 2008-2010 for 13 months the CCTV in the city office followed number and population density using the wildlife corridor. Based on the monitoring Korean raccoon dog(Nyctereutes procyonoides) and water deer(Hydropotes inermis), Korean hare(Lepus coreanus), Korean yellow-weasel(Mustela sibirica), Korean squirrels(Sciurus vulgaris) used most often and counting 884 individuals.. This counts over 73% of targeted species and also verifies the project objectives. In future the monitoring should be increased and identify behavioral patterns focusing on ecological networks of previously fragmented habitats.

Differences in Field Sign Abundance of Mammal Species Around the Roads in Baekdudaegan Mountains

  • Hur, Wee-Haeng;Lee, Woo-Shin;Choi, Chang-Yong;Park, Young-Su;Lee, Chang-Bae;Rhim, Shin-Jae
    • Journal of Korean Society of Forest Science
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    • v.94 no.2 s.159
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    • pp.112-116
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    • 2005
  • This study was conducted to obtain the information of distribution, protection and management for mammal species in fragmented forest areas around the road from June 2002 to May 2003 in 9 study sites of Baekdugdaegan mountains, Korea. Field signs of twelve mammals, moles Molera robusta, Korean hares Lepus coreanus, Manchurian chipmunk Tamias sibiricus, red squirrels Sciurus vulgaris, Korean racoon dogs Nyctereutes procyonoides, Siberian weasels Mustela sibirica, badgers Meles meles, otters Lutra lutra, Bengal cats Felis bengalensis, wild boars Sus scrofa, water deer Hydropotes inermis and roe deer Capreolus pygargus were recorded in this study. There were no differences in total number of species between 50 m areas and 50-100 m areas from road in snow and non-snow season. Number of mammals' field signs were different in non-snow season between both areas. Red squirrels and Siberian weasels were more abundant in 50 m areas, and Korean hares and Manchurian chipmunks were in 50-100 m areas. Habitat using pattern of mammal species may be affected by the road. Reasonable road construction and maintenance would be needed for protection and management of wildlife and their habitats.

How Do Landscape and Road Barriers Affect Road Crossing of Multihabitat Mammals (경관과 도로침입 방어막이 범서식지 포유류종의 도로 횡단에 미치는 영향 분석)

  • BYUN, Ye-Seul;KWON, Ji-No;KIM, Jeong-Hwan;SHIN, Moon-Hyun;LEE, Sang-Don
    • Journal of the Korean Association of Geographic Information Studies
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    • v.19 no.3
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    • pp.89-101
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    • 2016
  • This study examined spatial disposition of wildlife highway mortality using road-kill GIS database and Naver panoramic 360 degree views to find out which habitat and road variables most influenced road-kill numbers for each mammal species and how the landscape and road elements are connected on highway. Road-kills on Yeongdong(YD) and Jungbu highway(JB) generally tended to be higher in natural barren, grassland and cropland due to its value of preferred habitats of nocturnal and multihabitat species like water deer(Hydropotes inermis argyropus), raccoon(Nyctereutes procyonoides) and hare(Lepus coreanus). Land cover in YD showed no difference between species (p=0.165) while JB did by species (p=0.001). This may be explained by disparate landscape between mountain and urban or the fact that YD in long term operation might have enabled consistent crossing pattern compared to JB experiencing continuous extension works which may in turn have deviated the road crossing. Although road-kill prevention effect of local topography alone was appreciable, compared to less significant or ineffective fence and guardrail, gentle slope declining in a direction to the road turned out to offset the preventive effect of juxtaposed fence. Furthermore, green patches on road near intersection were deemed a visual stepping stone facilitating wildlife attempted crossing and local roads juxtaposed with a highway were especially left defenceless to road-kill without road barriers.