• The Korean Journal of Ecology
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• v.23 no.1
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• pp.33-37
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• 2000

Mass loss and changes of mineral nutrients during decomposition of Lepista nuda for 7 weeks from October 7 to December 28 in 1998 were investigated in an oak stand in Kongju, Korea. Content of nitrogen, phosphorus, potassium, calcium and magnesium of the fresh L. nuda was 67.8, 4.1, 47.3, 0.4 and 1.5 ㎎/g, respectively. Content of nitrogen, phosphorus and potassium in L. nuda were much higher than those in leaf litter. After 7 weeks, remaining mass was 35%. Nitrogen, phos- phorus and potassium increased till 5 weeks and then decreased rapidly, however, calcium and magnesium steadily increased during the experimental period. Nitrogen and phosphorus showed a short period of immobilization, and calcium showed no immobilization period during decomposition. After 7 weeks, remaining N, P, K, Ca and Mg was 26.6, 37.5, 28.5, 35.0 and 91.0% of the initial content, respectively. Nutrients could be relocated spatially during the formation and decomposition of fruiting body of Basidiomycetes.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.105-113
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• 1995

The mycelia of Lepista nuda were isolated from the different basidiocarps, collected from the forests of Robinia pseudo-acaciae and mixed forests of R. pseudo-acaciae and Quercus serrata. On the different woods, the growths of mycelia collected were compared with those transferred from Applied Mycology Division, RDA. Also, the measurements of dry weight loss, as growth of mycelia, were correlated to the productions of carbon dioxide on the different wood substrates. The growth of mycelia was observed to be best on the woods of R. pseudo-acaciae; followed by the growth on the woods of Morus alba. The growth rate of mycelia appeared to be various, depending on the different kinds of wood substrates. The soild-cultures of the mycelia on the sawdust of the R. pseudo-acaciae woods and the grains of sorghum and corn were conducted, but the basidiocarps were not obtained from these. Therefore, this mycelium collected was believed to be important as new mushroom utilizing the different woods resources instead of Q. serrata.

• 한국생태학회:학술대회논문집
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• 1999.05a
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• pp.112.1-112.1
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• 1999

• Journal of Life Science
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• v.11 no.5
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• pp.496-501
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• 2001

This study was carried out to investigate the cultural characteristics and the genetic characteristics in Lepists nuda. The mycelia cultural characteristics which include the specificity for pH, carbon source, nitrogen source and medium were studied using petridish culture and liquid media culture respectively. The genetic characteristics were also investigated using random amplified polymorphic DNA (RAPD) analysis. To find out the mycelial growth rate by various medium, mycelia was cultured for 25 days in PDA, YM, MEA, GPB and Yamanaka. The Yamanaka was superior to the other media in supporting the mycelial growth. pH6 produced the best result in the test of an optimal pH. In experiment for optimal carbon source, starch showa 46.8$\pm$1.7mm of diameter of mycelial colony as the best and for nitrogen source, yeast extract showed a good effect as well. In investigation for genetic characteristics, Lepists nuda was amplified by 7 primers among 10 primers and Pleurotus ostreatus was also amplified by 7 primers. From the RAPD analysis between species, the band patterns were different.

• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.256-262
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• 2011

A 20.9-kDa metalloprotease was isolated from dried fruiting bodies of the wild basidiomycete mushroom Lepista nuda. The N-terminal amino acid sequence of the protease was seen to be ATFVLTAATNTLFTA, thus displaying no similarity with the sequences of previously reported metalloproteases. The protease was purified using a procedure that entailed ion-exchange chromatography on CM-Cellulose, Q-Sepharose, and Mono S, and FPLC-gel filtration on Superdex 75. The protease functioned at an optimum pH of 7.0 and an optimum temperature of $50^{\circ}C$. It was also noted that the protease demonstrated a proteolytic activity of 1,756 U/mg toward casein. The $K_m$ of the purified protease toward casein was 6.36 mg/ml at a pH of 7.0 and with a temperature of $37^{\circ}C$, whereas the $V_{max}$ was 9.11 ${\mu}g\;ml^{-1}\;min^{-1}$. The activity of the protease was adversely affected by EDTA-2Na, suggesting that it is a metalloprotease. PMSF, EGTA, aprotinin, and leupeptin exerted no striking inhibitory effect. The activity of the protease was enhanced by $Fe^{2+}$, but was curtailed by $Cd^{2+}$, $Cu^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ ions. The protease also exhibited inhibitory activity against HIV-1 reverse transcriptase with an $IC_{50}$ value of 4.00 ${\mu}M$. The $IC_{50}$ values toward hepatoma Hep G2 and leukemia L1210 cells in vitro were 4.99 ${\mu}M$ and 3.67 ${\mu}M$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.317-322
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• 2005

This study was carried to investigate the inhibition effects of Lepista nuda mushroom extracts on the growth of the human cancer (HepG2, KATOⅢ, AGS) cells. The fraction Ⅰ of Lepista nuda mushroom extracts strongly inhibited to 71.4-91.8% for the growth of cancer cells. The growth of cancer HepG2, KATOⅢ and AGS cell which treated with 0.5 and 1㎎/mL of hot water fraction I was inhibited to 71.4% and 85.5%, 45.0% and 86.6%, 71.6% and 90.7% respectively. The growth of cancer cell HepG2, KATOⅢ and AGS which treated with 0.5 and 1㎎/mL of microwave fraction I was inhibited to 79.8% and 85.2%, 78.6% and 88.9%, 85.8% and 91.8%, respectively. The inhibition ratios of cancer cell growth were higher by microwave extracts than hot water extracts. And the more fraction were insoluble for water, the more inhibition ratios of cancer cell growth were lower.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1309-1314
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• 2006

This study analyzed the contents of vitamin C, polyphenol compounds, and evaluated the inhibitory activities against xanthine oxidase and tyrosinase of Lepista nuda extract prepared by the reflux and microwave-assisted methods. The vitamin C content of the fresh L. nude was $9.92{\pm}0.02$ mg/100 g. The content of phenolic compounds in the fresh L. nuda was $1.87{\pm}0.02\;mg/g$. The ethanol extracts prepared by reflux method and microwave-assisted method showed the highest phenol contents of $12.06{\pm}0.12\;mg/g\;and\;11.84{\pm}0.17\;mg/g$, respectively. The inhibitory rates on xanthine oxidase and tyrosinase of ethanol extracts prepared by the microwave assisted method showed the highest value of 99.78% and 30.30% at the concentration of $3,000{\mu}g/mL$. The inhibitory activities on xanthine oxidase and tyrosinase were increased depending on the extract concentration.

• The Korean Journal of Mycology
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• v.26 no.4 s.87
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• pp.589-593
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• 1998

Extracts from 65 species of mushrooms in mount Chiak were screened for their fibrinolytic activities. Extracts from Armillariella mellea, Calocybe sp., Lepista nuda and Trichaptum abietinum showed to have almost twice of activity of plasmin 1.5 U/ml, 193%, 213%, 198%, and 193% respectively. Collybia maculata showed 98% of activity, and Coprinus comatus and Lepiota sp. 56% and 58% activities, respectively. Other two, Agrocybe sp. and Stropharia rugosoannulata were less than 10% of activities.

• Mycobiology
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• v.39 no.2
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• pp.133-136
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• 2011

Mycelial growth and survival ratio of ectomycorrhizal fungi were determined after storage at $-70^{\circ}C$ for 1, 3, or 6 mon. Seventeen of 23 ectomycorrhizal fungi did not survive after storage for more than 6 mon, whereas Cenococcum geophilum, Lepista nuda, and some species of Rhizopogon and Suillus did survive.

• Food Science and Preservation
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• v.13 no.3
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• pp.375-381
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• 2006

This study was carried out to analyze the nutritional component of Lepista nuda in order to estimate its nutritional and functional values. The content of moisture were $90.90{\pm}0.09%$ carbohydrate $4.34{\pm}0.07%$, crude protein $3.70{\pm}0.03%$, ash $0.55{\pm}0.04%$, and crude fat $0.47{\pm}0.01%$, respectively The reducing sugar was $636.17{\pm}7.50mg$. Total amount of free sugar was 97.32 mg, which were mainly composed of galactose (51.98 mg) and trehalose (22.48 mg). In the results of mined analysis, the content of K was the highest as $103.10{\pm}0.18mg$, followed by $56.01{\pm}0.20mg$ of Mg. The total content of hydrolyzed amino acid was 310.39 mg which consist of essential amino acids including valine (25.40 mg), leucine (22.52 mg), lysine (21.34 mg) and 198.89 mg of non-essential amino acids including glutamic acid (60.72 mg), aspartic acid (30.17 mg), and alanine (29.72 mg), as the major amino acids. free amino acids were 220.86 mg which were composed of essential amino acids (36.21 mg) inclusive of isoleucine (21.57 mg) and threonine (11.20 mg) and non-essential amino acids (184.65 mg) including glutamic acid (42.78 mg) and aspartic acid (32.44 mg) as major amino acids. Total content of amino acid derivatives was 187.85 me and that of hydroxyproline was highest as 121.50 mg.