• Molecules and Cells
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• 제28권4호
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• pp.347-363
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• 2009

The 15,389-bp long complete mitogenome of the endangered red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined in this study. The start codon for the COI gene in insects has been extensively discussed, and has long remained a matter of some controversy. Herein, we propose that the CGA (arginine) sequence functions as the start codon for the COI gene in lepidopteran insects, on the basis of complete mitogenome sequences of lepidopteran insects, including P. bremeri, as well as additional sequences of the COI start region from a diverse taxonomic range of lepidopteran species (a total of 53 species from 15 families). In our extensive search for a tRNA-like structure in the A+T-rich region, one $tRNA^{Trp}$-like sequence and one $tRNA^{Leu}(UUR)$-like sequence were detected in the P. bremeri A+T-rich region, and one or more tRNA-like structures were detected in the A+T-rich region of the majority of other sequenced lepidopteran insects, thereby indicating that such features occur frequently in the lepidopteran mitogenomes. Phylogenetic analysis using the concatenated 13 amino acid sequences and nucleotide sequences of PCGs of the four macrolepidopteran superfamilies together with the Tortricoidea and Pyraloidea resulted in the successful recovery of a monophyly of Papilionoidea and a monophyly of Bombycoidea. However, the Geometroidea were unexpectedly identified as a sister group of the Bombycoidea, rather than the Papilionoidea.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.193-197
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• 2014

To investigate the degree of individuals' likability of insects, a survey was conducted with students and adults living in Korea. Overall, for insect-related questions, positive responses were higher than negative ones. Among the positive responses, the personal rearing of insects scored lower than others. The unconditional dislike of insects scored highest in the negative responses. A significant differences and correlations were found between males and females, students and adults, people experienced in insect-related events and those who were not. In particular, previous experience in insect-related events significantly influenced insect likability. Subjects preferred coleopteran and lepidopteran insects to others. However, this preference of insects differed by gender: Male participants preferred coleopteran insects to lepidopteran insects, while female participants preferred lepidopteran insects to coleopteran ones. No significant differences in the preference of insects were found by participants' job, residence, or experience in insect-related events. These findings suggest that gender is the most important factor to consider in selecting insects to be used as therapeutic tools in mental health care.

• 한국응용곤충학회지
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• 제61권3호
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• pp.481-496
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• 2022

주요 생활권 수목인 벚나무류, 단풍나무류, 느티나무, 철쭉류, 무궁화에 대한 효과적인 방제 전략 수립에 필요한 기초 자료 확보를 위해, 해당 수종별 나비목 곤충의 발생 양상을 2019년부터 2021년까지 조사하였고, 2020년 곤충의 발생 시기와 밀도를 조사하였다. 확인된 곤충으로는 벚나무류에서 14과 76종, 단풍나무류에서 8과 40종, 느티나무에서 10과 43종, 철쭉류에서 7과 28종, 무궁화에서 4과 10종이 발생하고 있음을 확인하였다. 그중 미국흰불나방은 느티나무를 제외한 모든 수목에서, 매미나방은 무궁화를 제외한 모든 수목에서 발생이 확인되었다. 발생밀도 조사 결과, 왕벚나무는 미국흰불나방, 갈색뿔나방이 많았으며, 느티나무는 느릅애기잎말이나방이, 무궁화는 점노랑들명나방의 발생이 가장 많았다. 미국흰불나방과 매미나방은 경남지역보다 경기지역에서 밀도가 높게 조사되어 지역별로 방제 전략을 달리해야 할 것으로 생각된다. 왕벚나무는 확인된 나비목 곤충 종수가 가장 많고, 광식성인 종이 많아 주의가 필요한 반면, 단풍나무는 나비목 곤충 피해가 크지 않는 것으로 파악되었다. 생활권 수목은 사람과 근접한 거리에 있어, 생활권 수목의 나비목 해충의 방제를 위해서는 페로몬을 이용하여 발생시기와 발생량을 예찰함으로써 농약 살포를 최소한으로 줄이는 것이 바람직할 것이다.

• 환경생물
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• 제25권1호
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• pp.42-55
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• 2007

We investigated the species richness of lepidopteran insects (excluding butterflies and microlepidoptera) of Mt. Seungdal-san, Muan-gun, Jeollannam-do, Korea, from 2003 to 2005 using two portable light traps. A total of species comprised 423 species belongs to 17 families were identified. The family Noctuidae with 209 species was dominant in the area followed by Geometridae with 132 species. Based on the number of individuals collected, Geometridae was the highest mostly due to an ennomine species (Alcis angulifera). Monthly fluctuations of moth species richness present an M-shaped curve with two high peaks at June and August. Comparisons of species diversity of yearly captures revealed that the diversity of 2003 was higher than that of 2004, but the diversities of between 2003 and 2005, and between 2004 and 2005 were no different.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권1호
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• pp.25-33
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• 2005

Recently a cDNA clone of apoLp-III from Hyphantria cunea was isolated and subjected to computational analysis to compare with other available sequences. Multiple sequence alignments were carried out using the amino acid sequences of apoLp-III from six insects. It was found that the H. cunea apoLp-III has relatively high sequence identities to Spodoptera litura ($69.5\%$), Manduca sexta ($66.8\%$), Galleria mellonella ($65.1\%$), Bombyx mori N4 ($54.3\%$) but less identity to Locusta migratoria ($18.3\%$). The amino acid composition was compared with other insects using EXPASY tools; it shows that alanine (Ala), glutamine (Gln), leucine (Leu) and lysine (Lys) are the major amino acid components of apoLp-III in H. cunea as well as other lepidopterans. Homology modeling performed using PSI-BLAST (PDB template M. sexta) reveals that the apoLp-III molecules consist of five, long amphipathic alpha helical bundles with short loops connecting the helices and shows homology with other insects. Phylogenetic analysis shows that the orthopteran apoLp-III represented by locust was most distantly related to the lepidopteran insects.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.75-75
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• 2003

1. To compare a full cDNA sequence of hemolin, a bacteria-induced protein of lepidopteran insects, from 4 geographically different strains of Bombyx mori. 2. To determine developmental profiles of hemolin gene expression in Bombp mori. (omitted)

• Journal of Microbiology
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• 제45권6호
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• pp.553-557
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• 2007

In order to detect and identify the most toxic Bacillus thuringiensis strains against pests, we isolated a B. thuringiensis strain (Bn1) from Balaninus nucum (Coleoptera: Curculionidae), the most damaging hazelnut pest. Bn1 was characterized via morphological, biochemical, and molecular techniques. The isolate was serotyped, and the results showed that Bn1 was the B. thuringiensis serovar, kurstaki (H3abc). The scanning electron microscopy indicated that Bn1 has crystals with cubic and bipyramidal shapes. The Polymerase Chain Reactions (PCRs) revealed the presence of the cry1 and cry2 genes. The presence of Cry1 and Cry2 proteins in the Bn1 isolate was confirmed via SDS-PAGE, at approximately 130 kDa and 65 kDa, respectively. The bioassays conducted to determine the insecticidal activity of the Bn1 isolate were conducted with four distinct insects, using spore-crystal mixtures. We noted that Bn1 has higher toxicity as compared with the standard B. thuringiensis subsp. kurstaki (HD-1). The highest observed mortality was 90% against Malacosoma neustria and Lymantria dispar larvae. Our results show that the B. thuringiensis isolate (Bn1) may prove valuable as a significant microbial control agent against lepidopteran pests.

• 한국응용곤충학회지
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• 제61권1호
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• pp.35-50
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• 2022

본 종설에서는 벼와 옥수수, 콩과작물, 감자, 들깨, 참깨에 대한 주요 나비목 해충 22종의 성페로몬 조성에 대해 국내외에서 연구된 결과들을 비교하면서 성충 예찰 수단으로 활용 가능한지를 분석하여 간단하게 정리하였다. 이 중 4종의 국내 개체군에서는 성페로몬샘 추출물로부터 성페로몬 성분들이 동정되고, 야외실험을 통해 그 조성이 규명되었다. 다른 3종에 대해서는 국내 개체군을 이용한 페로몬 화합물의 화학적 동정과정은 없었으나, 보고된 성페로몬 성분들을 이용한 실내 및 야외에서의 세밀한 조성실험을 통해 국내 개체군에 대한 최적 예찰조성이 선발되었다. 일부 종에 대해서는 최적 예찰미끼 개발을 위해 성페로몬샘에서 발견되는 미량성분의 역할에 대해 검토할 필요가 있다.

• Animal cells and systems
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• 제1권3호
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• pp.457-462
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• 1997

Antibacterial activity was induced in the haemolymph of the common cutworm, Spodoptera litura by the artificial injection of E, coli Ek132. Antibacterial peptides were purified from the immunized haemolymph by heat treatment, ion-exchange chromatography, gel filtration chromatography, and reverse phase FPLC, and their physicochemical characteristics were investigated. These purified antibacterial peptides designated as spodopsin la and Ib were named after Spodoptera litura. Spodopsin la and Ib had the apparent molecular masses of 3, 823 Da and 3, 886 Da, respectively, and about 20% of the sequences had basic amino acids, such as lysine and arginine but no cysteine. Also, spodopsin l was confirmed to be a new member of cecropin family having a similar amino acid sequence to cecropin of lepidopteran insects, such as Bombyx mori and Hyalophora cecropia. The purified spodopsin was active against gram-positive as well as gram-negative bacteria.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.146-146
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• 2002

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