• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.764-769
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• 2000

The lipase gene(lip) from Bacillus stearothermophilus was recombined in vitro by utilizing the DNA shuffling technique. After four rounds of shuffling, transformation, and screening based on the initial rate of clear zone formation on a tricaprylin plate, a clone (M10) was isolated, the cell extract of which showed about 2.8-fold increased lipase activity. The DNA sequence of the mutant lipase gene (m10) showed 3 base changes, resulting in two cryptic mutations and one amino acid substitution: S113($AGC{\rightarrow}AGT$), L252 ($TTG{\rightarrow}TTA$), and G275E ($GGA{\rightarrow}GAA$). SDS-PAGE analysis revealed that the increased enzyme activity observed in M10 was partly caused by high expression of the m10 lipase gene. The amount of the expressed G275E lipase was estimated to comprise as much as 41% of the total soluble proteins of the cell. The maximum velocity ($V_{max}$) of the purified mutant enzyme for the hydrolysis of olive oil was measured to be 3,200 U/mg, which was 10% higher than that of the parental (WT) lipase (2,900 U/mg). Its optimum temperature for the hydrolysis of olive oil was $68^{\circ}C$ and it showed a typical $Ca^{2+}$-dependent thermostability, properties fo which were the same as those of the WT lipase. However, the mutant enzyme exhibited a high enantiospecificity towards (S)-naproxen compared with the WT lipase. In addition, it showed increased hydrolytic activity towards triolein, tricaprin, tricaprylin, and tricaproin.

• 한국안전학회지
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• 제30권6호
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• pp.1-6
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• 2015

The actual condition is that environmental pollution due to the development of various industries has recently become a serious issue. An interest in improving the gas mileage is rising due to an increase in the number of vehicles in the era of high oil price in particular. In order to solve this problem, priority should be given to light-weight design of car body, However, at present, a design method enabling the conventional steel plate to be replaced is direly needed in order to guarantee passengers' safety according to excessive light-weight design of car body. In this study, in order to apply a design method that could realize fuel savings and environmental pollution prevention through an improvement in gas mileage together with meeting the safety requirements for vehicles, it was supposed that CFRP/Al composites member would be used as primary structural member. And to this end, it was intended to obtain optimum design data by experimentally implementing external impulsive load applied to the car body. According to results of impact test of CFRP/Al composites member, a collapsed shape of folding, crack, and bending occurred. So, it was possible to find that energy was observed. And in case of specimen having an angle of $90^{\circ}$ in the outermost layer and stack sequence of $[90^{\circ}{_2}/0^{\circ}2]s$, its collapsed length was shown to be short. Therefore, it was possible to find that the absorbed energy was shown to be higher by 20% or above at the maximum.

• Journal of Periodontal and Implant Science
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• 제51권2호
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• pp.88-99
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• 2021

Purpose: Direct intraoral scanning and superimposing methods have recently been applied to measure the dimensions of periodontal tissues. The aim of this study was to analyze various correlations between labial gingival thickness and underlying alveolar bone thickness, as well as clinical parameters among 3 tooth types (central incisors, lateral incisors, and canines) using a digital method. Methods: In 20 periodontally healthy subjects, cone-beam computed tomography images and intraoral scanned files were obtained. Measurements of labial alveolar bone and gingival thickness at the central incisors, lateral incisors, and canines were performed at points 0-5 mm from the alveolar crest on the superimposed images. Clinical parameters including the crown width/crown length ratio, keratinized gingival width, gingival scallop, and transparency of the periodontal probe through the gingival sulcus were examined. Results: Gingival thickness at the alveolar crest level was positively correlated with the thickness of the alveolar bone plate (P<0.05). The central incisors revealed a strong correlation between labial alveolar bone thickness at 1 and 2 mm, respectively, inferior to the alveolar crest and the thickness of the gingiva at the alveolar crest line (G0), whereas G0 and labial bone thickness at every level were positively correlated in the lateral incisors and canines. No significant correlations were found between clinical parameters and hard or soft tissue thickness. Conclusions: Gingival thickness at the alveolar crest level revealed a positive correlation with labial alveolar bone thickness, although this correlation at identical depth levels was not significant. Gingival thickness, at or under the alveolar crest level, was not associated with the clinical parameters of the gingival features, such as the crown form, gingival scallop, or keratinized gingival width.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권2호
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• pp.25-37
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• 2014

The volcanic type of geothermal water is linked intimately to active or potentially active volcanoes and takes place near the plate boundaries. In contrast to the volcanic type, the geothermal water in Korea has a non-volcanic origin. Korea's geothermal water is classified into the residual magma (RM) type and deep groundwater (DG) type according to the criterion of $35^{\circ}C$. This study reviewed the relationship between the physical and chemical features of the 281 geothermal water sources in South Korea in terms of the specific capacity, water temperature, and chemical compositions of two different basements (igneous rock and metamorphic rock) as well as the geological structures. According to the spatial relationship between the geothermal holes and geological faults, the length of the major fault is considered a key parameter determining the movement to a deeper place and the temperature of geothermal water. A negligible relationship between the specific capacity (Q/s) and temperature was found for both the RM type and DG type with the greater specific capacities of the RM- and DG-igneous types than the RM- and DG-metamorphic types. No relationship was observed between Q/s and the chemical constituents ($K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$, $Zn^{2+}$, $Cl^-$, $SO_4{^{2-}}$, $HCO_3{^-}$, and $SiO_2$) in the DG-igneous and DG-metamorphic types. Furthermore, weak relationship between temperature and chemical constituents was found for both the RM type and DG type.

• 한국과학교육학회지
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• 제20권1호
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• pp.1-11
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• 2000

본 연구에서는 초 중등 학생들의 눈금 읽기 능력과 측정도구의 용도 및 단위에 대한 개념을 조사했다. 초등학생 72명, 중1 학생 69명, 중3 학생 80명의 학생을 대상으로 수행 평가 및 지필 평가를 실시했다. 눈금읽기 능력을 평가하기 위해서 개별 수행 평가를 실시했는데, 학생 개개인은 서류 봉투의 가로 세로 길이, 물의 온도, 물의 부피 등을 실험실에서 주로 사용하는 주어진 측정 도구를 사용하여 측정했다. 측정 도구 및 단위와 관련된 개념을 평가하기 위해서는 그림과 사진이 그려진 질문지를 사용했다. 눈금 읽기 능력 면에서 각 학년마다 적지 않은 측정오차를 내는 학생이 있음이 드러났으며 특히 질량이나 부피 측정의 경우 그 수가 더욱 증가했다. 측정 도구에 대한 학생의 개념 조사 결과 학생들은 물리량 개념이 정확하지가 않아 측정 도구가 측정할 수 있는 물리량을 제대로 지적하지 못했다. 또한 단위에 대해서도 혼동이 있어서 특히 질량, 무게, 부피나 힘의 단위 등을 어려워하고 있음을 알 수 있었다.

• 한국어병학회지
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• 제26권2호
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• pp.59-64
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• 2013

This study aimed to develop an algorithm to determine the optimal injection position in olive flounder, Paralichthys olivaceus when a vaccine is injected into the fish by using a vision-based automatic equipment measruing the total length (TL), width and weight of the fish. Over a 5-month period, 500 olive flounders were examined to analyze the relation of the fish size and to the shape and location of its abdomen, using radiography, a ruler plate and scale. There were significant correlations between the TL and the shape and location of the abdomen. The abdomen was located 0.232TL - 2.7221 mm ($R^2$=0.8787) from the end of the mouth of the fish. The height and width of the abdomen in the fish were 0.1292TL + 1.8768 mm ($R^2$=0.7935) and 0.183TL-5.9791 mm ($R^2$=0.8641), respectively. The injection point in the abdominal region avoiding organs was determined by calculating the center of gravity of the abdomen volume. This can be expressed as g (0.2759TL - 2.0965, 0.1295TL - 4.2325) on the basis of TL line coordinates. The injection point by the expressed coordinates had errors for the x and y axes as 12.15 mm and 8.28 mm, respectively. These were enough to use the algorithm to injection for the equipment. This automated method to determine the position of any part in the fish can also be used for other purposes, for example, intramuscular injection or auto-tagging of fish.

• 대한기계학회논문집B
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• 제21권1호
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• pp.165-173
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• 1997

The present study investigates heat/mass transfer for flow through perforated plates for application to combustor wall and turbine blade film cooling. The experiments are conducted for hole length to diameter ratios of 0.68 to 1.5, for hole pitch-to-diameter ratios of 1.5 and 3.0, for gap distance between two parallel perforated plates of 1 to 3 hole diameters, and for Reynolds numbers of 60 to 13, 700. Local heat/mass transfer coefficients near and inside the cooling holes are obtained using a naphthalene sublimation technique. Detailed knowledge of the local transfer coefficients is essential to analyze thermal stress in turbine components. The results indicate that the heat/mass transfer coefficients inside the hole surface vary significantly due to flow separation and reattachment. The transfer coefficient near the reattachment point is about four and half times that for a fully developed circular tube flow. The heat/mass transfer coefficient on the leeward surface has the same order as that on the windward surface because of a strong recirculation flow between neighboring jets from the array of holes. For flow through two perforated plate layers, the transfer coefficients on the target surface (windward surface of the second wall) affected by the gap spacing are approximately three to four times higher than that with a single layer.

• 한국지반공학회논문집
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• 제15권2호
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• pp.105-127
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• 1999

쏘일네일링 공법은 지하굴착 및 사면보강에 그 적용성이 점차 확대되고 있으나, 도심지 특히 상부에 연약한 토사층이 존재하는 경우, 또한 시공여건등에 따라 네일의 설치길이가 제한되는 경우에는, 벽체변위 및 지표침하를 최소화시킬 수 있는 방안이 필요시 된다. 이와 같은 경우에, 변위 및 침하를 최소화시키는 주목적과 더불어 전체적인 안정성 증대도 동시에 도모할 수 있는 프리텐션 쏘일네일링 굴착벽체 시스템이 효율적이며, 이에 대한 시공도 국내의 경우에 극히 제한적으로 이루어진 바 있다. 그러나 본 프리텐션 쏘일네일링 굴착벽체 시스템에 대한 체계적인 해석절차 및 설계기법은 아직까지 정립된 바 없는 실정이며, 또한 프리텐션을 가하지 않는 일반 쏘일네일링공법의 경우에도, 전단 및 휨모멘트에 대한 안정성 확보를 위해 요구되는 숏크리트 전면벽체의 두께 및 와이어매쉬량의 산정 등에 대해서 명확한 해석절차가 제시되어 있지 않은 실정이다. 따라서 본 연구에서는, 쏘일네일링 굴착공법에 대해 해석 및 설계 측면에서의 미흡한 점을 보완함과 동시에 향후 적용 가능성이 클 것으로 판단되는 프리텐션 쏘일네일링 시스템에 초점을 두어 학문적 기초에 입각한 설계 및 시공을 유도하기 위해, 관련 해석절차 및 설계기법을 체계화하여 제시하였다. 제시된 내용은, 1) 프리텐션 쏘일네일링 시스템의 굴착단계별 최대긴장력 결정 및 안전해석기법, 2) 요구되는 숏크리트 전면벽체의 두께 및 와이어매쉬량과 첫단 네일의 한계설치깊이 등을 결정하기 위한 해석절차, 또한 3) 전면벽체의 관입전단파괴 가능성에 대한 신뢰도 분석기법 등이다. 예측결과를 실제의 시공사례와 비교\ulcorner분석하여, 본 연구 제시 절차 및 기법의 적합성을 확인하고자 하였다. 또한 제시기법을 이용해 프리텐션 네일링 시스템의 효용성 분석과, 이외에도 다이레이턴시 각 및 영향원 반경 등 주요인자에 대한 영향 및 FLAC$^{2D}$ 프로그램을 이용해 프리텐션 쏘일네일링 시스템의 발생변위 억제효과 등을 분석하였다.다.

• 대한두개안면성형외과학회지
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• 제10권1호
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• pp.33-36
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• 2009

Purpose: Fractures of the mandibular condylar area are common injuries that account for 29% to 40% of fractures of the facial bones and represent 20% to 62% of all mandibular fractures. Currently 3 main methods are being used in the treatment of mandibular subcondylar fractures: closed reduction; open reduction and internal fixation; Endoscopic reduction and internal fixation. Each method has its proponents and opponent as well as advantages and disadvantages, and indications for each vary among surgeons. There are six approaches of open reduction: submandibular, retromandibular, preaurilcular, postauricular, intraoral, transparotid approach. Among them, transparotid approach has been described for subcondylar exposure with dissection in the direction of facial nerve fibers to expose the bone through the parotid gland. This approach carries the risk of a parotid glandular fistula as well as facial nerve injury but has the advantage of being directly over the fracture site. We report safety and efficacy of surgical treatment using a transparotid approach for direct plating. Methods: A 43-year-old man sustained multiple facial bone fractures by driver traffic accident. Mandibular subcondyle was fractured and dislocated internally. We performed open reduction and internal fixation by transparotid approach. Fractured site was fixed by titanium mini plate & screw. We applicated arch bar for approximately 3 weeks. Results: Follow-up length was about 5months. Scar of surgical incision was indistinct, there was no symptoms and signs of facial nerve and parotid gland injury, and maximal mouth opening was measured 49.5 mm. Conclusion: Transparotid approach has high risks of facial nerve and parotid gland injury, but paradoxically it is the most effective technique in saving facial nerve. Open reduction and internal fixation of mandibular subcondylar fracture by transparotid approach with precise and versed procedure, best outcome can be expected.

• 건축역사연구
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• 제20권3호
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• pp.23-38
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• 2011

The Axes and Adzes are the oldest tools since the beginning of human history which is used to cut the tree and make part of architecture such as boards, square timber etc. Nowadays, these old woodworking tools especially "Jagui(자귀)" (adze) has been almost disappeared at the working site of residential and cultural properties. It is necessary to study Korean traditional woodworking tools to keep Korean traditional skill and technology. It has been reviewed the change of shape of "Jagui(자귀)" (adze) from before Samhan (삼한) period to Joseon Dynasty through excavated relics and paintings and summarize as follows: Based on excavation relics of Dahori, both plate type blade and pocket type blade are used for "Tokki(도끼)" (axe) and "Jagui(자귀)" (adze). The excavated "Jagui(자귀)" (adze) from Jeongbaikri 356 tomb, near Pyongyang is prototype of "Jagui(자귀)" (adze) used nowdays which has almost same shape as well as the insert method of blade and handle. The auxiliary handle is inserted to blade and the main handle is inserted to the auxiliary handle which is different from the method of Chinese and Japanese "Jagui(자귀)" (adze). The length of handle of "Jagui(자귀)" (adze) until late Joseon Dynasty is short by which we assume it is used for a sitting position as hand held Jagui and from that time long handle has been introduced for a standing position which is called "Sunjagui(선자귀)". "Jagui(자귀)" (adze) has been used since the beginning of human history but it is almost disappeared at the most of woodworking site which is the crisis of Korean traditional architecture and we have to do something to keep it.