• 대한수의학회지
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• 제36권1호
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• pp.195-207
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• 1996

To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from erythrocytes of Korean cattle, The previous studies on the probe of T sergenti had resulted in two probes as KTS1 and KTS3 DNA fragment. Nucleotide sequence of both ends of the KTS1 and KST3 were determined in order to design primers for polymerase chain reaction. A pair of an uper primer(5'-CCTCTTGAAGTCATCCATGT-3'; nucleotide position 48) and a lower primer(5'-CACTGAGCTG GAAAGAGCTA-3'; nucleotide position 156) in pKTS1 were synthesized. The anticipated PCR product was 128bp in length. To examine the sensitivity of the PCR, KTS1 DNA and purified T sergenti DNA were serially diluted by tenfolds with distilled water. The primers were sensitive enough to detect 4ag of the authentic template DNA and 4fg of the purified T sergenti DNA by PCR. Furthermore, when the blood was serially diluted by two-folds with 0.9% saline, the pair could detect up to 0.00029%(about 164 parasites in $10{\mu}l$ of blood) of T sergenti infection in bovine erythrocytes by PCR. In a comparison of microscopic and PCR detection of T sergenti in the same samples from Chonbuk area, 47 and 51 out of 70 sample(67.1%) were positive by the former and by the latter method, respectively.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1266-1271
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• 2017

Lactobacillus fermentum strain JDFM216, isolated from a Korean infant feces sample, possesses the ability to enhance the longevity and immune response of a Caenorhabditis elegans host. To explore the characteristics of strain JDFM216 at the genetic level, we performed whole-genome sequencing using the PacBio system. The circular draft genome has a total length of 2,076,427 bp and a total of 2,682 encoding sequences were identified. Five phylogenetically featured genes possibly related to the longevity and immune response of the host were identified in L. fermentum strain JDFM216. These genes encode UDP-N-acetylglucosamine 1-carboxyvinyltransferase (E.C. 2.5.1.7), ErfK/YbiS/YcfS/YnhG family protein, site-specific recombinase XerD, homocysteine S-methyltransferase (E.C. 2.1.1.10), and aspartate-ammonia ligase (E.C. 6.3.1.1), which are involved in peptidoglycan synthesis and amino acid metabolism in the gut environment. Our findings on the genetic background of L. fermentum strain JDFM216 and its potential candidate genes for host longevity and immune response provide new insight for the application of this strain in the food industry as newly isolated functional probiotic.

• 대한간호학회지
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• 제20권1호
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• pp.50-60
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• 1990

The purpose of this study was to investigate factors influencing role adaptation in newly employed nurses and the consistency between role expectations before employment and after one year of employment. The results of this study may contribute to strategy development towards positive role adaptation in newly employed nurses. The data used in this study were collected from 111 nurses, the sample was drawn from all newly employed professional nurses working at Y Medical Center in 1987. The data were collected longitudinally in the 2nd - 3rd weeks, the 3rd month and the 12th month after employment. The results of this study were as follows : 1. The degree of change the importance of factors influencing role adaptation and the length of time after employment were investigated. the result showed that the degree of change was the greatest within the first 3 months after employment. Important factors related to role adaptation were working conditions, the environment of the assigned ward and the work load. These factors always displayed high scores without any great change over time. New employees put more importance on practical factors which were obtained through experience in their jobs rather than on ideal factors Which they had considered more important while in school. 2. Consistency between role expectation before employment and after 12 month of employment was investigated. The highest consintency item was the expectation about the variety of Patients, and the highest inconsistency was the expectation about their own welfare. An average score of 69.07 points was achieved from a maximum of 125 points for the 25 items. showing that expectations for role development before employment were not fully satisfied. In conclusion the administraor should assess the initial expectation at the time of employment of new nurses and she / he should make clear to the new nurses that these expectations may not be realistic. In this way the administrator can provide more satisfactory conditions towards the expectations of the new nurses and help them towards positive role adaptation and reduction of role conflict. Newly employed nurses have high and unrealistic expectations about socialization to the profession from their nursing educational program. It is suggested that a transitional training program should be planned and carried out for newly employed nurses.

• 대한간호학회지
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• 제21권3호
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• pp.383-395
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• 1991

This study was to investigated the factors influencing the decisions of nurses to resign. The purpose was to contribute information useful for the effective management of nurse staffing resources. The specific objectives were as follows : 1. To investigate the level of job satisfaction of nurses who resign. 2. To analyze the relationship of job expectations and the decisions to resign. 3. To investigate the major reason for nurses' resignations. 4. To identify the relationship of the general characteristics of the nurses and the factors influencing their decisions to resign. 5. To analyze the relationship of the intention to resign and the factors influencing the decisions to resign. The sample consisted of 90 nurses who worked at Y hospital in Seoul from march. 1987 to Feb. 1988 and who had resigned. The instruments used for this study were slavitt et al's Index of Work Satisfaction Scale and a job expectations scale developed by the researcher Analysis of data was done using frequency, t-test and ANOVA. The results of study were as follows : 1. There was no difference in the job satisfaction level of nurses who resigned compared with nurses in active service but the subcategory interaction score was lower and the subcategory salary score was higher than for nurses in active service. 2. Low job expectation scores influenced the decision to resign. 3. The major reason for resigning were personal problems such as marriage, childbirth, and relocation of family. 4. The length of employment and the number of different service areas of the nurses who resigned were associated with the job satisfaction level. 5. The nurses who had have an intention to resign while in active service had lower scores for job satisfaction and the job expectation score was a greater influence on the decision to resign. In conclusion, the factors influencing the decisions of nurse to resign were job satisfaction, job expectations and intention to resign while still in active service.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.312-317
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• 2002

Indigenous lactobacilli were isolated from vaginas of Korean women for possible use in ecological treatment of bacterial vaginosis. Vaginal swab samples were obtained from a gynecological clinic and streaked on Rogosa SL agar plates to select the most predominant lactobacilli in each sample. The preliminary identification of the isolates as lactobacilli was based on microscopic observation of Gram-positive rod-shaped cell morphology. The initial characterization was performed on 108 isolates in terms of their cell surface hydrophobicity (CSH), antimicrobial activity, and hydrogen peroxide (H₂O₂) production capability, and 10 isolates were then selected for further molecular identification. For a rapid procedure to identify lactobacilli, polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses of the l6S rRNA genes were applied. The 10 selected lactobacilli and 9 different reference strains of Lactobacillus spp. were characterized by PCR-RFLP where the amplified l6S rDNA was digested with 7 different restriction endonucleases prior to analysis. DNA sequencing of the 16S rRNA gene of one particular isolate, KLB 46, that had been identified as L. crispatus by the PCR-RFLP analysis, further confirmed its identity as L. crispatus.

• Geomechanics and Engineering
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• 제24권6호
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• pp.573-588
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• 2021

The interaction of cracks and water significantly affects the fracture mechanism of rocks. In this study, laboratory tests were conducted using sandstone samples containing a single fissure to explore the hydro-mechanical behaviors in the failure process of pre-cracked rocks. The internal crack characteristics were also analyzed using X-ray CT scanning. The results show that the confining pressure has the greatest effect on the mechanical properties (e.g., strengths, elastic modulus, and Poisson's ratio), followed by the fissure inclination and water pressure. At a lower fissure inclination, the confining pressure may control the type main cracks that form, and an increase in the water pressure increases the number of anti-wing cracks and the length of wing cracks and branch cracks. However, the fracture behaviors of samples with a higher fissure inclination are only slightly affected by the confining pressures and water pressures. The effect of fissure inclination on the internal crack area is reduced with the propagation from the fissure tips to the sample ends. The fissure inclination mainly affects the value of permeability but not affect the trend. The impact of pre-existing fissure on permeability is smaller than that of confining pressure and water pressure.

• Nuclear Engineering and Technology
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• 제54권10호
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• pp.3587-3594
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• 2022

A distributed high-speed data acquisition and monitoring system for the RF negative ion source at Huazhong University of Science and Technology (HUST) is developed, which consists of data acquisition, data forwarding and data processing. Firstly, the data acquisition modules sample physical signals at high speed and upload the sampling data with corresponding absolute-time labels over UDP, which builds the time correlation among different signals. And a special data packet format is proposed for the data upload, which is convenient for packing or parsing a fixed-length packet, especially when the span of the time labels in a packet crosses an absolute second. The data forwarding modules then receive the UDP messages and distribute their data packets to the real-time display module and the data storage modules by PUB/SUB-pattern message queue of ZeroMQ. As for the data storage, a scheme combining the file server and MySQL database is adopted to increase the storage rate and facilitate the data query. The test results show that the loss rate of the data packets is within the range of 0-5% and the storage rate is higher than 20 Mbps, both acceptable for the HUST RF negative ion source.

• Imaging Science in Dentistry
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• 제44권3호
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• pp.193-198
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• 2014

Purpose: The present study was aimed to investigate the variation of soft palate morphology in different age and gender groups. The correlations of radiographic velar length (VL), velar width (VW), pharyngeal depth (PD), and Need's ratio with soft palate variants were also studied in the North Indian subpopulation. Materials and Methods: The study sample consisted of 300 subjects aged between 15 and 45 (mean: 31.32) years. The velar morphology on lateral cephalograms was examined and grouped into six types. The results obtained were subjected to a statistical analysis to find the correlation between variants of the soft palate with gender and different age groups. Results: The most frequent type of soft palate was leaf shaped (48.7%), and the least common was crook shaped (3.0%) among both the genders and various age groups, showing a significant correlation. The mean VL, VW, and PD values were significantly higher in males and significantly correlated with the types of soft palate. A significant correlation was observed between the mean VL, VW, PD, and Need's ratio with various age groups, showing an inconsistent pattern with an increase in age. The types of soft palate, gender, and Need's ratio were also significantly correlated, with an overall higher mean value of the Need's ratio among female subjects and the S-shaped soft palate. Conclusion: The knowledge of a varied spectrum of velar morphology and the variants of the soft palate help in a better understanding of the velopharyngeal closure and craniofacial anomalies.

• 대한치과교정학회지
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• 제13권2호
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• pp.177-183
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• 1983

The soft tissue covering of the face plays an important role in facial esthetics, speech and other physiologic functions. Thus, it is recognised by all clinical orthodontists that success of orthodontic treatment is closely related to the changes in soft tissues of the face. The purpose of this study was to evaluate the changes of bony and soft tissues in prepost treatment of Angle's Class III malocclusion. The sample consisted of 18 males and 37 females, pretreatment age of 9 years to 11 years. For this study 11 landmarks were plotted, 14 linear length, 4 soft tissue thickness and 2 angles were measured. The obtained results were as follows; 1. In the linear measurements of bony and soft tissue changes, A, Is, Ss, Ls and Li were located more anteriorly in both sexes. However Si and B showed more remarkable anterior movement in female. 2. In the comparison of the changes of the soft tissue thickness, Ss and Li in male subjects and Ss in female subjects increased. 3. In the degree of correlation between changes in the soft tissue profile and changes in the skeletal profile, Is: Ls, Il: Li and B: Si in both sexes had significant correlations. However A:Ss had remarkable significant correlation in female. 4. There were significant correlations between change in ${\angle}A$ and change in ${\angle}B$ in all sexes. 5. There were little correlation between changes in distance difference of Is and Ii and Change in distance difference of Ls and Li in all sexes.

• 한국기계가공학회지
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• 제10권3호
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• pp.1-6
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• 2011

This study focused on the localization of swivel type tube couplers, which all depend on imports. In this study, a computer application analysis was performed using a finite element method as a preliminary study. In the major developments related to the objective of this study, the air brake system produced by car makers represents a different in the installation point of an air tank according to the type of cars or in the length and direction of its hoses and that leads to cause lots of problems. For solving such problems, the design of the major elements in a swivel type tube coupler was analyzed using a finite element method, and its validity was also verified. In the process that verifies the validity of this study, it was necessary to investigate how much external force affects the desorption of the tube support, which is the most important element in swivel type tube couplers. For achieving the investigation, a pressure test was implemented for the tube support according to the Federal Motor Vehicle Safety Standards(FMVSS). In the results of the pressure test, all samples satisfied the FMVSS. In addition, several tests were implemented by installing the sample of the developed swivel type tube coupler to an actual vehicle. In particular, rotation tests with various angles were applied by welding the swivel type coupler to an air tank through an argon welding process. In the results of the installing test for an actual vehicle, it was verified that the designed structure was determined as a structure that is able to endure the eccentric torque and deformation pressure applied to several directions that are the major problems in such fixed type tube couplers. Therefore, in the comparison of the performance of the developed product with the product of PARKER, it was possible to verify that the localized swivel type tube coupler developed in this study shows more excellent than that of the existing products by PARKER.