• Journal of Plant Biotechnology
• /
• v.39 no.3
• /
• pp.121-126
• /
• 2012

To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.19 no.1
• /
• pp.61-71
• /
• 2016

The purpose of the research is to identify the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria as leguminous woody plants for the cut-slope revegetation. To investigate the coverage ratio and appearance frequency, we divided Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria into five treatment groups with various quantities of $0.0g/m^2$, $1.0g/m^2$, $2.4g/m^2$, $3.8g/m^2$, and $5.2g/m^2$. For each treatment group, we mixed the identical quantities of herbaceous flowers (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena and Pennisetum alopecuroides) seeds and cool-season turfgrasses (Festuca arundinacea and Poa pratensis) seeds. In this result, as the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria increased in the spray, the coverage ratio for leguminous woody plants appeared to increase whereas the coverage ratio decreased in herbaceous flowers and cool-season turfgrasses. However, when the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria exceeded $3.8g/m^2$ in the spray, the coverage ratio of leguminous woody plants rather decreased compared to four treatment groups less than $3.8g/m^2$ seeding ratio. Based on the longitudinal data of coverage ratio in five treatment groups, we observed the gradual process of a short-term succession in which the dominant species shifted in the following order: First, cool-season turfgrasses; Second, herbaceous flowers; Third, leguminous woody plants. Comparing the appearance frequency of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria, Lespedeza cyrtobotrya appeared more frequent in 2014 whereas Indigofera pseudo-tinctoria appeared to be relatively more frequent in 2015. As a result, Indigofera pseudo-tinctoria was discovered to be a dominant species among woody plants. In this study, we observed that the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria was $2.4g/m^2{\sim}3.8g/m^2$. Moreover, the coverage ratio of 29.1%~35.4% and appearance frequency of 4.6plants~5.8plants were found in the condition of optimal seeding quantity.

• Natural Product Sciences
• /
• v.4 no.4
• /
• pp.203-214
• /
• 1998

• Research in Plant Disease
• /
• v.24 no.4
• /
• pp.348-352
• /
• 2018

A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed for the detection of Clover yellow vein virus (ClYVV), Peanut mottle virus (PeMoV), and Tomato spotted wilt virus (TSWV), which were recently reported to infect soybean and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected and further evaluated to estimate the detection limits of uniplex, duplex, and multiplex RT-PCR. The multiplex RT-PCR assay could be a useful tool for the field survey of plant viruses and the rapid detection of ClYVV, PeMoV, and TSWV in leguminous plants.

• Journal of Plant Biology
• /
• v.22 no.1_2
• /
• pp.5-14
• /
• 1979

In several leguminous plants such as acasia, arrowroot and bushclover, growth rate and contents of nitrogen, phosphorus and potassium in the tissues and the variation in the culture media were determined. In water cultrue which was free of added nutrients, nitrogen was found to be largely in the form of nitrate(NO3-N). This NO3-N is believed to be the result of nitrification from NH4-N which was apparently released form the plants. From the studies of organ culture with root segments, the amount of nitrogen released and absorbed was found to be proportional to the amount added to the mediuim. Especially, in the N-plot, the amount of nitrogen absorbed by the tissue reached more than 90% of the amount supplied to the medium already in early stage. On the contrary, in the amount free plot, the amount of nitrogen released from the tissue was lower than the minimum level in the N-plot. The amount of total N and P in the cultured tissue was found to be influenced by the amount of nitrogen addedin the medium. However, the amount of K in the tissue was not related to the nitrogen level in the medium, but rather it was influenced by the amount of added potassium. These findings present little difference in the metabolic pattern among the three species plants studied, and suggest that the woody leguminous plants have some common features in tehir metabolic pattern.

• The Korean Journal of Mycology
• /
• v.20 no.3
• /
• pp.204-215
• /
• 1992

From 12 August to 21 November in 1991, 65 soil specimens were collected from nineteen leguminous plant roots throughout nine locations of four provinces. They were sieved to collect and identify the arbuscular mycorrhizal spores (four genera, 21 species). The species of Glomus were most commonly (47.6% of all observations) found, but the species of Gigaspora (about 4.8% of all observations) occurred infrequently or rarely rather than the species of the other genera. The most common arbuscular mycorrhizal species at nine locations were Acaulospora laevis and Glomus laminated spores cf. macrocarpus var. macrocarpus. All of nineteen legume plant species collected were found to be associated with the arbuscular mycorrhizal fungi . Cassia mimosoides var. nomame and Kummerowia striata in legume plants had the great frequency of arbuscular mycorrhizal fungi associated with their roots. The number of isolated spores were ranged from 0.3 to 10.0 spores per 10g of soil. The species diversity of arbuscular mycorrhizal spores at the disturbed areas were calculated to be bigger than those in the natural vegetation areas.

• Journal of Microbiology and Biotechnology
• /
• v.34 no.3
• /
• pp.570-579
• /
• 2024

Root-nodule nitrogen-fixing bacteria are known for being specific to particular legumes. This study isolated the endophytic root-nodule bacteria from the nodules of legumes and examined them to determine whether they could be used to promote the formation of nodules in other legumes. Forty-six isolates were collected from five leguminous plants and screened for housekeeping (16S rRNA), nitrogen fixation (nifH), and nodulation (nodC) genes. Based on the 16S rRNA gene sequencing and phylogenetic analysis, the bacterial isolates WC15, WC16, WC24, and GM5 were identified as Rhizobium, Sphingomonas, Methylobacterium, and Bradyrhizobium, respectively. The four isolates were found to have the nifH gene, and the study confirmed that one isolate (GM5) had both the nifH and nodC genes. The Salkowski method was used to measure the isolated bacteria for their capacity to produce phytohormone indole acetic acid (IAA). Additional experiments were performed to examine the effect of the isolated bacteria on root morphology and nodulation. Among the four tested isolates, both WC24 and GM5 induced nodulation in Glycine max. The gene expression studies revealed that GM5 had a higher expression of the nifH gene. The existence and expression of the nitrogen-fixing genes implied that the tested strain had the ability to fix the atmospheric nitrogen. These findings demonstrated that a nitrogen-fixing bacterium, Methylobacterium (WC24), isolated from a Trifolium repens, induced the formation of root nodules in non-host leguminous plants (Glycine max). This suggested the potential application of these rhizobia as biofertilizer. Further studies are required to verify the N₂-fixing efficiency of the isolates.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.6
• /
• pp.689-696
• /
• 2015

The green manure crops are returned to soil when the plants are still green. The green manure crops play a role in reducing chemical fertilizers, improvement of soil fertility, reduction of soil erosion, weed control, and landscapes effect. Typical green manure crops are barley green manure, hairy vetch, chines milk vetch and clovers. We sowed 49 species of green manure crops in upland soil to increase the usage of green manure crops. We searched the effectiveness of green manure yield, C/N ratio, soil mulching, and landscape effect. The green manure crops that have $5tonha^{-1}$ or more of green manure yield (fresh weight) were 6 gramineous crops, 4 leguminous crops, and 3 others. Green manures yield in a short growth period was higher in gramineous crops than leguminous crops. Among the green manure crops, 3 gramineous crops, 12 leguminous crops, and 3 others were under 25 in C/N ratio. Seven gramineous crops, 8 leguminous crops, and 5 others were over 50% of soil covering rate. Green manure crops that have beautiful flowers were in total 16 kinds, such as 4 gramineous crops, 6 leguminous crops, and 6 others. In result, based on green manure yield and C/N ratio, excellent species in the green manure crops were 4 species (triticale, 2 kinds of hairy vetch, and lupin). Green manure crops that were effective at soil mulching were 20 species, like hairy vetch, oats, sorghum, phacelia, buckwheat, etc. Buckwheat, crotalaria, mustard plant, phacelia, and etc. could be available for landscape crops.

• Mycobiology
• /
• v.38 no.4
• /
• pp.286-294
• /
• 2010

Epicoccum purpurascens stain 5615 AUMC was investigated for its biocontrol activity against root rot disease caused by Pythium irregulare. E. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. The germination rate of the three species of legume seeds treated with a E. purpurascens homogenate increased significantly compared with the seeds infested with P. irregulare. No root rot symptoms were observed on seeds treated with E. purpurascens, and seedlings appeared more vigorous when compared with the non-treated control. A significant increase in seedling growth parameters (seedling length and fresh and dry weights) was observed in seedlings treated with E. purpurascens compared to pathogen-treated seedlings. Pre-treating the seeds with the bioagent fungus was more efficient for protecting seeds against the root rot disease caused by P. irregulare than waiting for disease dispersal before intervention. To determine whether E. purpurascens produced known anti-fungal compounds, an acetone extract of the fungus was analyzed by gas chromatography mass spectrometry. The extract revealed a high percentage of the cinnamic acid derivative (trimethylsiloxy) cinnamic acid methyl ester. The E. purpurascens isolate grew more rapidly than the P. irregulare pathogen in a dual culture on potato dextrose agar nutrient medium, although the two fungi grew similarly when cultured separately. This result may indicate antagonism via antibiosis or competition.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.52 no.2
• /
• pp.169-175
• /
• 2007

This experiment was carried out to compare weed occurrence and to evaluate the field performance of some leguminous plant species under no-weeding fallow condition during summer months from middle of June to middle of August. In 2005 and 2006, the first dominant weed species was Digitaria sanguinalis, and other major weed species, such as Cyperus amuricus, Portulaca oleracea, and Amaranthus retroflexus, were similar among treatments. In both years, total shoot dry weight of weeds was the highest In the control plot, and the lowest in the cowpea plot, indicating that cowpea most effectively suppressed the growth of weeds among treatments. When the growth of four different leguminous species were compared after seeding during middle of June in 2006, cowpea and rice bean grew vigorously under no-weeding condition during hot and humid summer months, while wild soybean and hairy vetch did not. Shoot dry weight was in the order of cowpea > rice bean > wild soybean > hairy vetch. In conclusion, cowpea is a leguminous species which can be used as a green manure cover crop during summer months under no-weeding and our climate condition. Further works are needed to use cowpea as a green cover crop during summer months for various cropping systems.