• Proceedings of the PSK Conference
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• 2002.10a
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• pp.320.2-320.2
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• 2002

Macrophages activated by lipopolysaccharide (LPS) are known to induce several proinflammatory proteins including COX-2. iNOS and TNF which produce chemical mediators involved in inflammatory response. Sophoricoside and its analogs (genistin, genistein and orobol) from Sophora japonica (Leguminosae) showed differential inhibitory effects on COX-1 and 2 activities. Sophoricoside and genistin shwoed IC50 values of 4 uM and 6 uM on COX-2 activity and of 1,497 uM and 135 uM on COX-1 activity, respectively. Genistein and orobol showed IC50 values of 3 uM on COX-2 activity and of 28 uM and 18 uM on COX-1 activity. respectively. Therefore. the legume isoflavonoids to be selective COX-2 inhibitors. However. sophoricoside and its analogs did not show inhibitory effects of COX-2, iNos and TNF transcripts. which were identified by the RT-PCR.

• Archives of Pharmacal Research
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• v.29 no.10
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• pp.821-825
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• 2006

Two isoflavone C-glucosides, puerarin (1) and PG-3 (2), a but-2-enolide, $({\pm})-puerol$ B (3), two isoflavone O-glucosides, daidzin (4) and genistin (5), and three pterocarpans, (-)-medicarpin (6), (-)-glycinol (7) and (-)-tuberosin (8), were isolated from a MeOH extract of the roots of Pueraria Iobata, using an in vitro bioassay based on the inhibition of the formation of advanced glycation end products (AGEs) to monitor chromatographic fractionation. The structures of 1-8 were determined by spectroscopic data interpretation, particularly by 1D- and 2D-NMR studies, and by comparison of these data with values in the literature. All of the isolates (1-8) were evaluated for their inhibitory activity on AGEs formation in vitro. Of these, puerarin (1), PG-3 (2), and $({\pm})-puerol$ B (3) exhibited more potent inhibitory activity than the positive control aminoguanidine.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.143-149
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• 1999

The phytochemical studies of the leaves of Caragana chamlagu were carried out as a sieries of the investigation of medicinal resources. The roots of Caragana chamlagu have been used as neuralgia, arthritis and migraine in the folk medicines of Korea. The methanolic extract of the leaves of Caragana chamlagu was suspended with water and then separated with chloroform. Compound I was isolated from precipitates of these water fraction by recrystalization. The aqueous fraction of MeOH extract was performed to column chromatography on Amberlite XAD-4 and Sephadex LH-20, and three compounds, compound II, compound III, and compound IV were isolated. The structures of the four compounds were elucidated by spectroscopic data of $^1H-NMR$, ^{13}C-NMR$, IR, and FAB-MS. Compound I-IV were tilianine ($acacetin-7-O-{\beta}-D-glycopyranoside$), rutin($quercetin-3-O-{\alpha}-L-rhamnopyranosy(1{\rightarrow}6)-{\beta}-D-glu-copyranoside$), $kaempferol-3-O-{\alpha}-L-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-D-galactopyranoside$, and apigetrin, ($apigenin-7-O-{\beta}-D-glycopyranoside$), respectively.

• Natural Product Sciences
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• v.10 no.6
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• pp.330-334
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• 2004

Four isoflavonoids and three flavonoids, and a gallotannin were isolated from the fruits of Sophora japonica (Leguminosae). Their structures were identified as genistein (1), sophoricoside (2), genistein-4'-O--L-rhamnopyranoside (3), $genistein-4'-O-{\alpha}-L-rhamnopyranosly-(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (4), $kaempferol-3-O-{\alpha}-D-sophoroside$ (5), $kaempferol-3-O-{\beta}-D-glucopyranosyl-(1{\rightarrow}2)-{\alpha}-L-rhamnopyranosly-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (6), rutin (7) and gallic acid $4-O-{\beta}-D-(6'-O-galloyl)-glucopyranoside$ (8) by chemical and spectroscopic analysis and comparisons with previously reported spectral data. Compounds 3 and 8 were isolated for the first time from this plant. Anti-oxidative activity was evaluated for the isolated compounds. 8 exhibited potent anti-oxidative activity against the radical scavenging ability of DPPH with the $IC_{50}$ value of $17.1\;{\mu}g/ml$.

• Korean Journal of Pharmacognosy
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• v.33 no.2 s.129
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• pp.110-115
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• 2002

Seven prenylated flavonoids and two pterocarpans together with a saponin were isolated from the roots of Echinosophora koreensis. They were identical with echinoisosophoranone, echinoisoflavanone, isosophoranone, sophoraisoflavanone A, kenusanone C, kenusanone A, sophoraflavanone D, 4-hydroxy-3-methoxy-(6aR,11aR)-8,9-methylenedioxy-pterocarpans, (-)-maackiain, and $3-O-{\alpha}-L-rhamnopyranosyl(1{\rightarrow}2)-{\beta}-D-galactopyranosyl(1{\rightarrow}2)-{\beta}-D-glucuronopyranosyl$ kuzusapogenol A. Among them, a pterocarpan, 4-hydroxy-3-methoxy-(6aR,11aR)-8,9-methylenedioxypterocarpan, and a saponin, $3-O-{\alpha}-L-rhamnopyranosyl(1{\rightarrow}2)-{\beta}- D-galactopyranosyl(l{\rightarrow}2)-{\beta}-D-glucuronopyranosyl$ kuzusapogenol A, were isolated for the first time from this plant.

• Korean Journal of Environment and Ecology
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• v.25 no.6
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• pp.836-845
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• 2011

This study aims feeding habit of the Korean water deer(Hydropotes inermis argyropus) using a visual analysis of rumen contents of 44 individuals. The samples were collected from Cheorwon(Gangwon province) and the eastern part of Jeonnam province. Based on the analysis, a total of 15 orders, 18 families and 13 species were identified. Among them, Solanceae(53.8%) and Graminease(30.3%) showed the highest proportions. Plants of 13 orders, 15 families and 10 species were found and forbs(70.21%) and Solanceae(54.9%) were most preferred in Cheorwon. Eight orders, 7 families and 4 species were found in the Eastern parts of Jeonnam province, and Gramineae(78.6%) and woody plants(14.70%) was most preferred. Among four seasons, Gramineae in spring(80.6%) and autumn(68.8%), Leguminosae(75.9%) in summer, and Solanceae(85.4%) in winter were the highest proportions.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.217-225
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• 2006

Among the seven taxa of the genus Vigna, Leguminosae vegetated in Korea peninsula, only two taxa, V. unguiculata subsp. unguiculata and V. vexillata var. tsusimensis, are recognized in Vigna. The rests are still remained in the genus Phaseolus in many floras and illustrations of Korea although they were placed into the genus Vigna by Verdcourt in 1970. There has been misidentification between V. angularis var. nipponensis and V. nakashimae because of their similar morphological characters and habit. It was also found that V. nakashimae has not been recorded in many floras. Objectives of this study are 1) to discuss the taxonomic differences between Vigna and Phaseolus, 2) to have a recommendation for using correct scientific names of Vigna taxa, 3) to discuss the taxonomic characteristics of the subgenus Ceratotropis, and 4) to provide a simple taxonomic key of V. angularis var. nipponensis and V. nakashimae.

• Journal of Life Science
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• v.21 no.7
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• pp.1060-1066
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• 2011

In order to investigate the variations on the ratio of mannose to galactose in the seeds of Phaseolus angularis, 17 local strains (Yangyang, Pyeongchang, Ganghwa, Pocheon, Geumsan, Seocheon, Jincheon, Danyang, Tongyeong, Sancheong, Gumneung, Wolseong, Wando, Gokseong, Okgu, Jangsu, Bukjeju), which are located from $33^{\circ}15'N$ to $38^{\circ}11'N$, were selected according to their latitudes and geographical distances. The seeds of these strains were collected and their contents of mannose and galactose were analyzed. Mannose contents in the seeds were variable, ranging from 17.071 mg/g at its highest (Jangsu) and 6.488 mg/g at its lowest (Geumsan). The contents of galactose also showed remarkable differences, ranging from 9.477 mg/g (Wolseong) to 19.877 mg/g (Jangsu). The local strains were classified into 3 variation types - coastal type I (Wando, Okgu, Bukjeju), the inland type (Jangsu, Weolseong, Danyang, Geumneung, Pyeongchang, Sancheong) and coastal type II (Ganghwa, Seocheon, Tongyeong, Jincheon), as well as 4 strange strains (Gokseong, Yangyang, Pocheon, Geumsan) according to the geographical climatic type and the ratio of mannose to galactose, which indicate the hardness of seeds in Leguminosae and ranged from 0.64 to 1.22. The variation types are very significant genecologically as evidence for microevolution related to natural and artificial selection in cultivated plants.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.1
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• pp.7-13
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• 2004

Mung beans (Vigna radiata L.) were used to investigate the roles of strontium in hypocolyl elongation under IAA regime during the germination. After imbibition in a medium with or without IAA, $Sr^{2+}$ stimulated IAA oxidation. Three to five fold increasing in IAA oxidase activity seems to be direct evidence of growth inhibition through $Sr^{2+}$. Furthermore, the accumulation of spermidinc and spermine by $Sr^{2+}$ in the range of 1 to 10 mM was observed. Spermidine levels were 2 to 3 fold higher than in control seedling grown without strontium. The increase in polyamine levels was observed on a g fresh weight basis. In conclusion, it was demonstrated that the inhibitory action of $Sr^{2+}$ is closely related with the IAA oxidation and polyamine biosynthesis.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.537-542
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• 2007

Sophora flavescens AITON (Leguminosae) is a typical traditional Korean medical herb considered to exhibit antibacterial, anti-inflammatory, and antipyretic effects, and is also used for the treatment of skin and mucosal ulcers, sores, diarrhea, gastrointestinal hemorrhage, arrhythmia, and eczema. In this study, the compound sophoraflavanone G was isolated from the dried roots of S. flavescens by bioassay-guided fractionation. We then investigated the effects of various concentrations of sophoraflavanone G on cell viability and the induction of apoptosis in KB cells after an incubation of 24 hr. The results were determined by the following methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-terazolium bromide (MTT) assay, Hoechst-33258 dye staining, flow cytometry (cell cycle), and Western blotting for caspase-3 and poly (ADP-ribose) polymerase (PARP). We found sophoraflavanone G induced the apoptosis of KB cells in a dose-dependent manner that was verified by DNA fragmentation, apoptotic bodies, the sub-G1 ratio, caspase-3 activity, and cleavage of PARP. These results suggest that sophoraflavanone G has potent anti-proliferative effects on human oral epidermoid carcinoma cells, with the induction of apoptosis.