• 한국자원식물학회지
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• 제34권6호
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• pp.527-535
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• 2021

Legumes have been important components of the human diet. They contain not only protein, starch, and dietary fiber, but also various phenolic compounds such as flavonoids and phenolic acids. The importance of phenolic compounds to human health is well known due to their antioxidant activities. In this study, three legumes (adzuki beans, common beans, and black soybeans) frequently cultivated in Korea were evaluated for their total phenolic content (TPC) and antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azinobis (3-ethylbenzothiazoline 6-sulfonate)), and FRAP (ferric reducing antioxidant potential) assays. In addition, correlations between agricultural traits and antioxidant activities of these three legumes were analyzed. Antioxidant activities assessed by DPPH, ABTS, and FRAP assays and TPC showed wide variations among legumes types and accessions. Among the three legumes, adzuki beans showed higher TPC and antioxidant activity than the other two legumes. In correlation analysis, seed size showed negative correlations with antioxidant activities and TPC. In principal component analysis and hierarchical clustering analysis, each of the three legumes was clearly separate. Results of this study can be used as basic information for developing functional materials for each legume. They can also help us understand the overall antioxidant activity of the three legumes.

• Nutrition Research and Practice
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• 제7권3호
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• pp.185-191
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• 2013

In previous studies, we found that the consumption of legumes decreased bone turnover in ovariectomized rats. The purpose of the present study is to determine whether the protective effects on bone mineral density (BMD) and the microarchitecture of a diet containing legumes are comparable. In addition, we aim to determine their protective actions in bones by studying bone specific gene expression. Forty-two Sprague-Dawley rats are being divided into six groups during the 12 week study: 1) rats that underwent sham operations (Sham), 2) ovariectomized rats fed an AIN-93M diet (OVX), 3) ovariectomized rats fed an AIN-93M diet with soybeans (OVX-S), 4) ovariectomized rats fed an AIN-93M diet with mung beans (OVX-M), 5) ovariectomized rats fed an AIN-93M diet with cowpeas (OVX-C), and 6) ovariectomized rats fed an AIN-93M diet with azuki beans (OVX-A). Consumption of legumes significantly increased BMD of the spine and femur and bone volume of the femur compared to the OVX. Serum calcium and phosphate ratio, osteocalcin, expression of osteoprotegerin (OPG), and the receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) ratio increased significantly, while urinary excretion of calcium and deoxypyridinoline and expression of TNF-${\alpha}$ and IL-6 were significantly reduced in OVX rats fed legumes, compared to OVX rats that were not fed legumes. This study demonstrates that consumption of legumes has a beneficial effect on bone through modulation of OPG and RANKL expression in ovariectomized rats and that legume consumption can help compensate for an estrogen-deficiency by preventing bone loss induced by ovarian hormone deficiency.

• Journal of Animal Science and Technology
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• 제46권3호
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• pp.437-442
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• 2004

콩과목초는 젖소의 중요한 단백질 공급원이며 토양에는 질소비료원이다. 본 시험은 콩과목초의 사초생산성과 품질을 비교하기 위하여 천안연암대학의 실습농장에서 실시하였다. 공시한 콩과목초 5초종은 2모작이 가능한 목초로 크림손 클로버(Trifolium incarnatum L.) 'Tibbee', 헤어리 베치(Vicia villosa Roth) 'Common', 레드클로버(Trifolium pratense L.) 'Kenland', 페르시안 클로버(Trifolium resupinatum L.) 'Leeton', 알팔파(Medicago sativa L.) 'Vernal' 이다. 콩과목초의 내한성은 크림손 클로버가 7점인 것을 제외하고는 공시품종 모두가 8점 이상으로 양호하였다. 콩과목초의 개화기는 크림손 클로버가 4월 23일로 공시초종 중에서 가장 빨리 개화하였으며, 헤어리 베치는 5월 13일, 레드 클로버는 5월 3일, 알팔파는 5월 12일에 개화하였다. 그러나 페르시안 클로버는 수확시까지 출수를 하지 않았다. 수확시 건물률은 크림손 클로버가 17.6%로 공시초종 중에서 가장 높았다. 건물 및 TDN 수량은 크림손 클로버와 헤어리 베치가 공시초종 중에서 가장 많앗다. 조단백질 함량은 헤어리 베치가 20.5%로 가장 높았다. NDF 및 ADF 함량은 페르시안 클로버가 35.5 및 25.0%로 가장 적었다. NDF와 ADF를 근거로 계산한 상대사료가치(RFV)는 헤어리 베치를 제외하고는 콩과목초 모두가 125이상으로 특급이었다. 페르시안 클로버는 공시초종에서 상대사료가치와 TDN이 가장 높은 양질의 콩과목초였다. 이상의 결과를 종합해 볼때 콩과목초 중 크림손 클로버와 헤어리 베치가 사초수량이 높았고, 페르시안 클로버가 품질이 우수하였다.

• Journal of Nutrition and Health
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• 제32권6호
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• pp.617-627
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• 1999

The present study was conducted to evaluate the usefulness of four kinds of legumes(black soybean: BS, yellow soybean: YS, green peas: GP, soybean curd residue: SCR) as a high-fiber supplement in the therapeutic diet for diabetic patients. Seven groups of normal and streptozotocin-induced diabetic rats were fed isocaloric experimental diets containing 8% dietary fiber from one of legumes for 6 weeks. The effects of legumes on the gastrointestinal function and diabetic symptoms in diabetic rats were also examined and compared with the effects of cellulose or pectin diet. Legume supplementations had no significant effects on alleviating typical diabetic symptoms such as polydipsia, polyphasia, polyuria, and urinary glucose excretion when compared with cellulose or pectin supplementations. The supplementations of legumes all resulted in remarkable changes in gastrointestinal functions: shortening of GI transit time and increases in fecal volume, fecal water and crude fat contents. Legume supplementations appeared to supress the damages of the small intestine villi and the enteritis symptoms in diabetic rats. GP led to regular villus patterns closer to normal villus. BS, YS and GP led to the enlargement of cecum in diabetic rats. In conclusion, the present studies have demonstrated that legumes may possess a significant physiological actions in the control of various diabetic conditions.

• 한국유기농업학회지
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• 제10권1호
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• pp.49-63
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• 2002

In order to supply 85% of total organic feed in ruminants and 80% in non-ruminants for organic animal production, nitrogen fixation ability of legume should be used in domestic roughages production. 50% of Europe organic farmer use intercropping legume in as green manure. This article is dealing with amount of biologically fixed nitrogen used by legumes and methods for estimating the transfer of biologically fixed N in rotation and separating the N benefit into fixed N and non-fixed N components are reviewed. Available data indicate that transfer amount of N to non legumes was from 50∼9.6(kg/ha) in legume-cereal rotations and proportion of legume N varied with seasons, 90% in summer, 50% in autumn. The important point in cropping system for legumes have to be included for organic forage production 6 year rotation is based on pasture system of 3 year pasture + 2 year annual(com, sudangrass), again pasture. Rye, barly and Italian ryegrass+legumes(vetch, crimson and pea) can be one of option in spring, com, soybean, sudangrass and Japanese bamyard millet would be seeded after spring harvest in the field. Farmer can make good use of rice paddy field as forge production potential area after harvesting rice. Italian, burly and rye+vetch and crimsonclover may be grown in autumn or spring time at the rice field.

• Asian-Australasian Journal of Animal Sciences
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• 제7권3호
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• pp.325-333
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• 1994

This study was undertaken at Gowa, South Sulawesi, Indonesia as part of a larger forage genetic resource evaluation project. The experimental program consisted of a field experiment where grasses and legume species were grown in monocultures and the yield, N content and rumen bag digestibility using goats were measured. The field experiment was conducted from December 1985 to October 1986. Eight species of legumes (Desmodium heterophyllum cv. John-stone, Desmodium triflorum from Gowa, South Sulawesi, Arachis sp. from Maiwa, South Sulawesi, Clitoria ternalea CPI 50973, Macroptilium atropurpureum ev. Siratro, Neonotonia wightii cv. Tinaroo, Centrosema pubescens CPI 58575, Centrosema plumeri CPI 58568) were grown as monocultures. After establishment all plants were cut to a uniform height of 5 cm, and subsequent cuts were made on regrowth after 14, 28, 42, and 56 days (cycle 1). Cycle 2 commenced towards the end of the wet season and continued for 157 days into the dry season. The highest yielding legumes were C. ternatea in the wet season and Arachis sp. in the dry season. The mean rumen bag dry matter digestibility (RBDMD) of legumes of 67.6% for leaf material (averaged over all cycles and ages) was 7.6% higher than for stem material. The RBDMD of Arachis was significantly higher than all other species. The RBDMD of all legumes declined with age. Calculation of yield of digestible DM (yield $\times$ RBDMD) showed that Arachis sp. was the best legume. The combination of plant "quality" with yield measures is a valuable adjunct to routine agronomic survey procedures in plant evaluation programs. Arachis sp. appears to offer considerable promise and should be more widely evaluated.

• 한국식품영양과학회지
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• 제30권5호
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• pp.770-776
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• 2001

방사선 조사 식품의 검지방법을 연구하기 위하여 국산 및 중국산 두류(대두, 땅콩, 적두, 녹두)를 대상으로 0.5~4kGy의 감마선을 조사하여 hydrocarbon류의 생성특성을 비교하였다. Hexane을 이용하여 추출한 지방질을 florisil column chromatography에 의해 hydrocarbon류를 분리시키고 GC-MS에 의해 분석하였다. 방사선 조사된 각종 두류의 hydro-carbon 생성특성은 다소 상이하였고, 두류의 지방산 조성에 영향을 받았다. 대두, 땅콩 및 적두에서는 0.5 kGy 혹은 그 이상으로 조사시켰을 때 16 : 3, 17 : 2, 16 : 2 및 17 : 1을 각각 확인할 수 있었으나, 녹두에서는 17 : 1이 검출되지 않았다. 국산 및 중국산 두류에서 방사선 조사 유래의 hydrocarbon류 생성량은 조사선량에 비례하였다. 그러나, 방사선 조사 유래 hydrocarbon류 생성량은 조사선량에 비례하였다. 그러나, 방사선 조사 유래 hydrocarbon류의 종류와 양에는 원산지별 차이가 나타나지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권10호
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• pp.1404-1410
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• 2012

In vitro experiments were conducted to evaluate the suitability of several mixtures of high tanniniferous non legumes with low tanniniferous legumes on in vitro gas production (IVGP), dry matter degradation, Ammonia-N, methane production and microbial population. Eight treatments were examined in a randomized complete block design using four non-legumes and two legumes (Carallia integerrima${\times}$Leucaena leucocephala (LL) (Trt 1), C. integerrima${\times}$Gliricidia sepium (GS) (Trt 2), Aporosa lindeliyana${\times}$LL (Trt 3), A. lindeliyana${\times}$GS (Trt 4), Ceiba perntandra${\times}$LL (Trt 5), C. perntandra${\times}$GS (Trt 6), Artocarpus heterophyllus${\times}$LL (Trt 7), A. heterophyllus${\times}$GS (Trt 8). The condensed tannin (CT) content of non legumes ranged from 6.2% (Carallia integerrima) to 4.9% (Ceiba perntandra) while the CT of legumes were 1.58% (Leucaena leucocephala) and 0.78% (Gliricidia sepium). Forage mixtures contained more than 14% of crude protein (CP) while the CT content ranged from 2.8% to 4.0% respectively. Differences (p<0.05) were observed in in vitro gas production (IGVP) within treatments over a 48 h period dominated by C. perntandra${\times}$G. sepium (Trt 6). The net gas production (p<0.05) was also high with Trt6 followed by A. heterophyllus${\times}$L. leucocephala (Trt 7) and A. heterophyllus${\times}$G. sepium (Trt 8). Highest (p>0.05) NH3-N (ml/200 mg DM) production was observed with the A. heterophyllus${\times}$G. sepium (Trt 8) mixture which may be attributed with it's highest CP content. The correlation between IVGP and CT was 0.675 while IVGP and CP was 0.610. In vitro dry matter degradation (IVDMD) was highest in Trt 8 as well. Methane production ranged from 2.57 to 4.79 (ml/200 mg DM) to be synonimous with IVGP. A higher bacteria population (p<0.05) was found in C. perntandra${\times}$G. sepium (Trt 6) followed by Artocarpus heterophyllus+G. sepium (Trt 8) and the same trend was observed with the protozoa population as well. The results show that supplementing high tannin non leguminous forages by incremental substitution of legume forage increased gas production parameters, NH3-N, IVDMD and microbial population in the fermentation liquid. Methane production was not significantly affected by the presence of CT or different levels of CP in forage mixtures. Among non legumes, Ceiba perntandra and Artocarpus heterophyllus performed better in mixture with L. leucocephala and G. sepium.

• 한국식품영양과학회지
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• 제39권9호
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• pp.1399-1404
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• 2010

잡곡의 이용 증진을 위하여 국산 잡곡 12종에 대한 이화학적 특성 및 항산화 활성을 분석한 결과는 다음과 같다. 조단백질 함량은 두류(20.60~34.47%)가 곡류(8.96~15.45%)에 비하여 높았으며, 조지방 함량은 대두와 흑태에서 각각 17.73 및 18.79%로 높았으며 그 밖의 잡곡류에서는 0.68~5.39% 범위였다. 잡곡의 주요 무기성분은 칼륨, 칼슘 및 나트륨 등 이었으며, 특히 두류에서 칼슘 및 칼륨 함량이 높았다. 지방산 조성은 불포화지방산이 66.79~84.84%로 많았으며, $\Omega$-3 지방산(linolenic acid) 함량은 두류에서 7.47~48.25% 범위로 곡류의 0.36~3.71%보다 높았다. 총 폴리페놀과 플라보노이드 함량은 두류의 80% 에탄올 추출물이 곡류보다 많았으며, DPPH 및 ABTS radical 소거능은 수수의 에탄올 추출물이 2 mg/mL의 농도에서 각각 62.60% 및 264.20 mg AA eq/g으로 가장 우수하였으며, 강낭콩 및 팥에서 높은 항산화 활성을 나타내었다. 이와 같은 잡곡별 성분함량 및 항산화 활성 결과는 기능성식품 개발을 위한 자료로서 활용될 수 있으리라 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.651-654
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• 2006

An experiment was conducted to investigate amino acid composition of three tropical legumes (Cooper (Glycine wightii), Tinaroo (Neonotonia wightii) and Siratro (Macroptilium atropurpureum)) at two different stages (pre-blooming and blooming stages). Chemical composition and totally 16 amino acids of these plants were analysed for comparison of their composition among species at different growing stages and characterizing the amino acid pattern of these legumes. Crude protein content of the plants ranged from 16% to 27% on a dry matter basis. The total amount of 16 amino acids analyzed in this experiment was highest at 89.7 mg/16 g N in Cooper at pre-blooming and lowest at 80.9 mg/16 g N in Glycine at blooming stage. Total amount of amino acids in each legume species tended to slightly decrease with their maturity but no statistical difference was found. The percentage of aspartic acid, glutamic acid and proline in the total amount of amino acids was dominant at 9% to 13%, and that of methionine was less than 1.6%. In this experiment it was concluded that three tropical legumes were rich in crude protein content and characterized by 16 different amino acids with lower sulfur-containing amino acid as methionine.