• Journal of Ecology and Environment
• /
• v.48 no.3
• /
• pp.296-307
• /
• 2024

Background: This study compares seed germination and seedling growth parameters of native Senegalia catechu with its closely related invasive Leucaena leucocephala in Nepal. For the comparison of seed germination percentage (GP), mean germination time (MGT), and Timson's index (TI), the seeds of both species were incubated under different light (photoperiod and dark), temperatures (30/20℃ and 25/15℃) and water stress conditions (-0.1, -0.25, -0.5, -0.75, and -1 MPa). The seedling emergence from different soil depths was also evaluated. The relative growth rate (RGR), root mass fraction (RMF), stem mass fraction, leaf mass fraction (LMF), and root-to-shoot ratio (RSR) of seedlings were also measured. Results: The seed length and mass of invasive L. leucocephala were higher than that of native S. catechu. The GP of S. catechu was higher at high temperature and photoperiod comparing to L. leucocephala. There was no difference in GP between two species under other light and temperature conditions. The MGT of S. catechu was shorter than that of L. leucocephala at both temperatures. Senegalia catechu exhibited higher TI than L. leucocephala, particularly at high temperatures. Water stress above -0.5 MPa reduced the GP and TI of both species and it was more pronounced in S. catechu than L. leucocephala. The seedling emergence percentage of L. leucocephala was higher than that of S. catechu. Both species exhibited comparable RGR and biomass allocations (RMF, LMF, and RSR). However, L. leucocephala had always greater values of shoot height, root length, leaf number and seedling biomass compared to S. catechu. Conclusions: Larger seeds may not always lead to higher seed GP. Some, but not all, seed germination and seedling growth traits can be useful to characterize invasive alien plant species. Invasiveness of L. leucocephala could be attributed to relatively high tolerance of seed germination to water stress, capacity to germinate from deeper soil, and larger seedling size compared to the confamilial native species.

• The Korean Journal of Pesticide Science
• /
• v.2 no.3
• /
• pp.21-27
• /
• 1998

Methanol extracts from 25 leguminous seeds were tested for their fungicidal activities toward six phytopathogenic fungi, using whole plant test in a greenhouse. The efficacy varied with both the plant pathogen and legume species used. At 5 mg/pot, potent fungicidal activities were produced from extracts of Cassia obtusifolia, Glycine max var. solitae, G. max var. yagkong, G. max var. hooktae, Phaseolus multiflorus, P. radiatus var. aurea, and Vigna sinensis against Botrytis cinerea, Puccinia recondita, and Erysiphe graminis. These seed extracts were highly effective against three B. cinerea strains resistant to carbendazim, procymidone, and diethofencarb. All leguminous seed extracts revealed weak or no fungicidal effect against Rhizoctonia solani, Pyricularia grisea, and Phytophthora infestans. As a naturally occurring fungicide, leguminous seed-derived materials described could be useful as new fungicidal products against various plant diseases induced by phytopathogenic fungi.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.54 no.3
• /
• pp.320-326
• /
• 2009

The crops showing hard seed character have high seed viability after seed storage for long period. The low germination rate due to hard seed coat, however, cause a problem of low seedling establishment in field condition. Three legumes used in the experiment, lablab bean (Dolichos lablab L.), asparagus bean (Vigna sesquipedalis L. Fruwirth), and soybean (Glycine max L. Merr.), showed low germination rate (26, 17, and 5%, respectively) due to thick and hard seed coat. In this study artificial treatment for breaking dormancy was tested in hard seeds. The effect of proper treatment was various depending on species. The germination rate of lablab bean was highly improved up to 94% by soaking into water for 24 hours. In the case of asparagus bean, the rate was increased up to 90% by soaking for eight hours near boiling water until it cools. The germination rate of small hard seed soybean was increase to 96% by soaking into concentrated sulfuric acid for 10 minutes. Ultrastructural change revealed by scanning electron microscope (SEM) reflects that the structure of micropyle was changed and water uptake was facilitated with all treatments tested in the experiment. Especially, sulfuric acid treatment resulted in the degradation of micropylar tissue. These results demonstrate that the artificial treatment including sulfuric acid and (hot) water soaking treatment for promoting water uptake can be applied to improve seed germination in legume seed with thick and hard seed coat.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.3
• /
• pp.246-251
• /
• 1989

A medicinal legume crop, Cassia tora L., was grown with four different seeding dates in 1986 field condition to find the flowering and pod setting characteristics. Initial flowering date and flowering duration were almost same between plants sown from April 20 to May 30, but those of plants seeded at June 20 were significantly delayed and shortened. Last flowering date, however, was not different between plants sown from April 20 to June 20. C. tora plants showed indeterminate flowering habit, and bloomed vigorously from fourth date after initial flowering. Numbers of flowers, ovules and pods per plant were decreased along with delaying seeding date, and ovule to flower ratio and pod to flower ratio were greatly decreased in June 20 seeding plot. Flowers bloomed on second week from initial flowering showed the highest number of ovules and pods, and flowers bloomed after early September did not develop to matured pods. Maximum values of pod length, number of seeds per pod and 1000-grain weight were obtained from flowers bloomed on second week from initial flowering of plants sown from April 20 to May 30, and first week in June 20 seeding plot.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.134-134
• /
• 2022

Mungbean is used for not only seed but sprout, so, consumption of mungbean has been on the rise in Korea. Life cycle of mungbean tends to be short among Legume. For that reason, Mungbean can be harvested for various cropping system and season per regions and farmers regardless of sowing date and harvesting date. So, Prior research is needed about growth characters and life cycle of mungbean per sowing period. Mungebean cultivar 'Dahyun' and 'Sanpo' supplied by Korea Agriculture Technology Promotion Agency(KOAT) is cultivated in wagner pot. Sowing period is proper time of seeding in Jeollanam-do and Gyeongsangnam-do that is major cultivation region of mungbean in korea from early May to mid July every 2 weeks. Length at maturity stage tends to increase from early May(sowing date: 4^th May) to early July(sowing date: 5^th July), but after that, It tends to decrease from mid July(sowing date: 19^th July). Number of branches and nods shows a similar trend. Length of pod has no tendency and no difference per sowing date. Number of pod per plants has also no tendency per sowing date. Test plots sowing in late May has the most number of pods.(Sanpo 22.9pods, Dahyun 16.8) Number of seeds per pod tends to increase to late May and Test plots sowing in mid July has the most number of seed per pod. In case of sowing at early May, Days of emergence is 7d. its summation of temperature is 132.2℃. After that, it tends to decrease to mid June. After mid June, Days of emergence is fixed to 3d. Average temperature growing up in this season, Summation of temperature from sowing to emergence takes the lowest point in test plots sowing in mid June.(Sanpo 88.6℃, Dahyun 88.6) Days of flowering tends to fasten from early May to mid July. Two cultivar shows same level. Days of maturity tends to fasten to mid June, after that tends to slow. In case that many research results about growth characters and life cycle mungbean per sowing period are drawn, it is expected that it result in increase of cultivation area and income of farmer.

• Journal of Plant Biotechnology
• /
• v.38 no.2
• /
• pp.176-185
• /
• 2011

Rice is one of the most important crop in the world, in particular for food resources. With its small genome size of 383 Mb, the Oryza sativa is a model plant for genome research. Indeed, it's grain provides human with a source of carbohydrates and proteins. Rice grain has relatively low protein contents (around 8%) compared to other legume seeds (around 40%). Osborne classified seed proteins into water soluble albumin, salt soluble globulin, alcohol soluble prolamin and acidic/alkaline solution soluble glutelin. Glutelin and prolamin are the major storage proteins in rice. For the gene expression study of seed storage proteins, we analyzed 33,192 EST clones at immature stages in a rice cultivar (Oryza sativa L. cv. 'Ilpum'). Based on the expression analysis, we cloned 11 glutelin genes and figured out the 8 genes are located on Chromosome 2. The expression of glutelin genes appears to be about 28.2% of total level in immature seeds. Interestingly, glu-04 is duplicated as inverted sequences on the same chromosomes as far 4.5 kb. Our results indicate that glutelin genes, evolutionarily, were replicated on the chromosome and thus expressed as specific manners. In a whole protein composition analysis, glu05 (type B7) contains the highest lysin contents (4.51%) among the 11 rice glutelin genes. It will be an interesting future work to increase lysin contents by the gene overexpressor strategy with the aim of improved diet nutritionally fortified.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.5
• /
• pp.667-673
• /
• 2010

Organic agriculture of especially medicinal crops has been interested and focused in taking the qualitative and safety agricultural products in order to maintain and improve the health and happiness of people. With respect to the organic agriculture relating to cropping system, objectives of this study were to examine the seedling establishment after seed germination and emergence and to investigate the early seedling growths of Astragalus membranaceus Bunge with treating two green manure crops, hairy vetch of legume and rye of gramineae, two different conditions of green manures, fresh and dry, and different seeding times. When the seeds were planted at once after treating the green manures with the fresh condition into the soil, the ratios of seedling establishment were very poor, less than 10% in hairy vetch and 20% in rye, respectively. They were increased gradually with delaying the seeding times after the treatments. With treating the green manures of dry conditions, the ratios of seedling establishment were up more than 50% in both hairy vetch and rye crops, showing that the damages of seedling establishment were more in hairy vetch than those in rye. The early seedling growths were slower in treating the green manures than those in the control, showing slow growth with fresh green manures compared to the dry. It would be very important and considered more to evaluate and utilize well the seed germination and the seedling establishment in cultivation of the organic agriculture using the green manure crops relating to the cropping system.