• Title/Summary/Keyword: Lee constant

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Batch and Continuous Culture Kinetics for Production of Carotenoids by ${\beta}$-Ionone-Resistant Mutant of Xanthophyllomyces dendrorhous

  • Park, Ki-Moon;Song, Min-Woo;Kang, Seog-Jin;Lee, Jae-Heung
    • Journal of Microbiology and Biotechnology
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    • v.17 no.7
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    • pp.1221-1225
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    • 2007
  • A ${\beta}$-ionone-resistant mutant strain isolated from the red yeast Xanthophyllomyces dendrorhous KCTC 7704 was used for batch and continuous fermentation kinetic studies with glucose media in a 2.5-1 jar fermentor at $22^{\circ}C$ and pH 4.5. The kinetic pattern of growth and carotenoid concentration in the batch fermentations exhibited a so-called mixed-growth-associated product formation, possibly due to the fact that the content of intracellular carotenoids depends on the degree of physical maturation toward adulthood. To determine the maximum specific growth rate constant (${\mu}_m$) and Monod constant ($K_s$) for the mutant, glucose-limited continuous culture studies were performed at different dilution rates within a range of $0.02-0.10\;h^{-1}$. A reciprocal plot of the steady-state data (viz., reciprocal of glucose concentration versus residence time) obtained from continuous culture experiments was used to estimate a ${\mu}_m$ of $0.15\;h^{-1}$ and $k_s$ of 1.19 g/l. The carotenoid content related to the residence time appeared to assume a typical form of saturation kinetics. The maximum carotenoid content ($X_m$) for the mutant was estimated to be $1.04\;{\mu}g/mg$ dry cell weight, and the Lee constant ($k_m$), which was tentatively defined in this work, was found to be 3.0 h.

Methodology for Extracting Trap Depth using Statistical RTS Noise Data of Capture and Emission Time Constant

  • Oh, Dong-Jun;Kwon, Sung-Kyu;Song, Hyeong-Sub;Kim, So-Yeong;Lee, Ga-Won;Lee, Hi-Deok
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.17 no.2
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    • pp.252-259
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    • 2017
  • In this paper, we propose a novel method for extracting an accurate depth of a trap that causes RTS(Random Telegraph Signal) noise. The error rates of the trap depth rely on the mean time constants and its ratio. Here, we determined how many data of the capture and emission time constant are necessary in order to reduce the trap depth error caused by an inaccurate mean time constant. We measured the capture and emission time constants up to 100,000 times in order to ensure that the samples had statistical meaning. As a result, we demonstrated that at least 1,000 samples are necessary to satisfy less than 10% error for trap depth. This result could be used to improve the accuracy of RTS noise analysis.

Flow Behavior and Performance Characteristics of Constant Air Volume Fan According to Different Hub Shape (허브 형상에 따른 정풍량 환기팬의 유동과 성능특성)

  • Lee, Ho-Ho;Choi, Hang-Cheol;Jung, Jae-Goo;Lee, Yoon-Pyo;Shin, Yoo-Hwan;Chung, Jin-Taek
    • The KSFM Journal of Fluid Machinery
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    • v.15 no.2
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    • pp.57-62
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    • 2012
  • The constant air volume flow fan can maintain constant flow rate to the wide range of exit pressure. Therefore, the use of this fan is increasing recently for ventilation of high building. Brushless DC motor is adopted to this fan because that has advantages of compactness and performance. But this type of motor protrude from impeller hub side to fan inlet. The Impeller inlet flow is influenced by size of this obstacle called hub. In this paper, the influence of hub shape on the fan performance characteristics are experimentally and numerically analyzed. CFX 12.0 is used to perform the fan internal flow analysis and numerical results are compared with the experiments. Depending on hub shape, internal loss is generated and the performance and efficiency are reduced. The best performance is occurred around $h/b_1$ = 0.25. The results of this study will be contribute to initial design of constant air volume flow fan development.

Rotor Time Constant Compensation for Vector-Controlled Induction Motor with DC Current Injection Method (직류전류 주입법에 의한 벡터제어 유도전동기의 회전자 시정수 보상)

  • Lee, Gyeong-Ju;Lee, Deuk-Gi;Jeong, Jong-Jin;Choe, Jong-U;Kim, Heung-Geun;No, Ui-Cheol;Jeon, Tae-Won
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.51 no.2
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    • pp.69-76
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    • 2002
  • To obtain a high performance in a vector controlled induction motor, it is essential to know the instantaneous position of the rotor flux which depends on the rotor time constant. But the rotor time constant mainly varies due to the temperature rise in the motor winding, so real time compensating algorithm is necessary. This paper proposes that it uses short duration pulses added to the constant flux command current and then resultant torque command current produced by speed controller is utilized for the rotor resistance estimation. This method has advantage with a low computational requirement and does not require voltage sensors. The proposed method is proved by simulations and experimentals.

Modified 𝜃 projection model-based constant-stress creep curve for alloy 690 steam generator tube material

  • Moon, Seongin;Kim, Jong-Min;Kwon, Joon-Yeop;Lee, Bong-Sang;Choi, Kwon-Jae;Kim, Min-Chul;Han, Sangbae
    • Nuclear Engineering and Technology
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    • v.54 no.3
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    • pp.917-925
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    • 2022
  • Steam generator (SG) tubes in a nuclear power plant can undergo rapid changes in pressure and temperature during an accident; thus, an accurate model to predict short-term creep damage is essential. The theta (𝜃) projection method has been widely used for modeling creep-strain behavior under constant stress. However, many creep test data are obtained under constant load, so creep rupture behavior under a constant load cannot be accurately simulated due to the different stress conditions. This paper proposes a novel methodology to obtain the creep curve under constant stress using a modified 𝜃 projection method that considers the increase in true stress during creep deformation in a constant-load creep test. The methodology is validated using finite element analysis, and the limitations of the methodology are also discussed. The paper also proposes a creep-strain model for alloy 690 as an SG material and a novel creep hardening rule we call the damage-fraction hardening rule. The creep hardening rule is applied to evaluate the creep rupture behavior of SG tubes. The results of this study show its great potential to evaluate the rupture behavior of an SG tube governed by creep deformation.

Relationship Between Enhancement of Electrostriction and Decrease of Activation Energy in Porcine Pancreatic Lipase Catalysis

  • PARK HYUN;LEE KI-SEOG;PARK SEON-MI;LEE KWANG-WON;KIM AUGUSTINE YONGHWI;CHI YOUNG-MIN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.3
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    • pp.587-594
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    • 2005
  • The contribution of electrostriction of water molecules to the stabilization of the negatively charged tetrahedral transition state of a lipase-catalyzed reaction was examined by means of kinetic studies involving high-pressure and solvent dielectric constant. A good correlation was observed between the increased catalytic efficiency of lipase and the decreased solvent dielectric constant. When the dielectric constant of solvents was lowered by 5.00 units, the losses of activation energy and free energy of activation were 7.92 kJ/mol and 11.24 kJ/mol, respectively. The activation volume for $k_{cat}$ decreased significantly as the dielectric constant of solvent decreased, indicating that the degree of electrostriction of water molecules around the charged tetrahedral transition state has been enhanced. These observations demonstrate that the increase in the catalytic efficiency of the lipase reaction with decreasing dielectric constant resulted from the stabilization of electrostatic energy for the formation of an oxyanion hole, and that this stabilization was caused by the increase of electrostricted water around the charged tetrahedral transition state. Therefore, we conclude that the control of solvent dielectric constant can stabilize the tetrahedral transition state, thus lowering the activation energy.

Evaluation of the Spring Constant of a Micro Coil Spring (마이크로 코일 스프링의 스프링 상수 평가)

  • Lee J. K.;Jeon B. H.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2001.10a
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    • pp.255-259
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    • 2001
  • The spring constant of a micro coil spring was measured by uniaxial tensile test. The inner diameter of it is $35{\mu}m$ and the pitch size is about $23{\mu}m$. A suing constant measurement system was developed. It consists of control units, load cell units, linear stages and several specially designed jigs and fixtures. Load and displacement are measured using a commercial load cell of 1000g capacity and a magnetic scale of $0.5{\mu}m$ resolution. In this study, a method to measure the spring constant of micro coil spring is presented and the relationship between misalignment of specimen and measurement error is discussed.

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NUMERICAL STUDY OF UNSTEADY HEAT TRANSFER ON MICRO HEATER UNDER HALF-CYCLE SINUSOIDAL HEAT LOAD (마이크로히터에서 반주기 정현곡선의 열부하에 의한 비정상 열전달 연구)

  • Kim, M.J.;Lee, H.J.
    • Journal of computational fluids engineering
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    • v.19 no.4
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    • pp.1-7
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    • 2014
  • A numerical study of transient conjugate heat transfer on micro heater in a micro-channel substrate under a sinusoidal heat load was conducted. It was found that the time constant is not affected by the maximum heating magnitude of the sinusoidal heat load. However, the time constant increases with low duration of the sinusoidal heating period and low Reynolds number. Moreover, there is a threshold where a heater temperature do not reach to time constant at low thermal diffusivity, low flow rate, and low pulse duration of the sinusoidal heating. The time constant should be considered for transient convective heat transfer under transient sinusoidal heat load in a micro heat sink.

A Modified Turbulent Porous Modeling for Numerical Analysis (수치해석을 위한 변형된 난류 다공성 모델링)

  • Chung, Kil-Yoan;Lee, Kwan-Soo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.6
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    • pp.875-882
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    • 2002
  • The modeling for turbulent flow through a porous media has not been confirmed because of a undetermined constant which appears in the governing equations. In present study, the turbulent porous modeling based on the local thermal equilibrium has been extended to the turbulent clear flow. A undetermined constant is also suggested by microscopic analysis. The microscopic analysis is performed in the flat tube with micro-channels, and it confirms that the undetermined constant is 0.99. It is shown that the results of the macroscopic analysis using confirmed constant agree well with those of the microscopic analysis with a maximum error of 3.5%.

Simulated Distribution Characteristics of Surface Temperature on Irradiating of a Laser

  • Lee, Young-Wook;Yeon, Sang-Ho
    • International Journal of Contents
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    • v.5 no.2
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    • pp.16-19
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    • 2009
  • In this paper, we concern about the distribution characteristics of surface temperature by the increment of time, diffusivity and heat flux on irradiating of a laser. The penetration depth corresponding to the induced constant heat flux or irradiated laser, is simulated by a computer algorithm. The distribution of temperature versus penetration depth for the variation of time and diffusivity is characterized at the constant heat flux and on irradiating of a laser. The temperature of constant heat flux at the fixed diffusivity or time, is decreased by the pattern of exponential function as the time t or diffusivity a is increased (a=10, 100, 1000). The temperature of constant heat flux is not changed but exponentially fixed with the increasing diffusivity and the fixed time. On the other hand, the temperature of laser at the fixed diffusivity or time is decreased linearly. Our results show that the characteristics of the simulated surface temperature in a semi-infinite solid are similar to the graphs on theoretical consideration.