• Title/Summary/Keyword: Lee Sam

Search Result 4,879, Processing Time 0.036 seconds

Studies on the Production of Fermented Feeds from Agricultural Waste Products (Part IV) -On the Production of Cellulase by Aspergillus niger and Trichoderma viride- (농산폐기물(農産廢棄物)에서 발효사료(醱酵飼料)의 생산(生産)에 관(關)한 연구(硏究)[제4보(第四報)] -Aspergillus niger와 Trichoderma viride에 의(依)한 Cellulase의 생산성(生産性)에 관(關)하여-)

  • Lee, Ke-Ho;Koh, Jeong-Sam;Lee, Kang-Hup
    • Applied Biological Chemistry
    • /
    • v.19 no.3
    • /
    • pp.139-144
    • /
    • 1976
  • In order to investigate the properties of enzymes from two strains of mold, reported in the previous paper, (1) studies have been made concerning the characteristics of cellulase of Aspergillus niger-SM6 and Trichoderma viride-SM10, and summarized as follows. 1. In the semi-purification the recovery of ${\beta}-glucosidase$ was the highest when 80-90% ethanol was used and 0.8 saturation of $(NH_4)_2SO_4$. 2. The characteristics of the semi-purified enzyme were as follows. Aspergillus niger-SM6 Trichoderma viride-SM10 Optimum pH 3.5 4.0 pH stability 3.0-6.0 3.0-6.0 Optimum temperature $60^{\circ}C$ $60^{\circ}C$ Heat stability below $60^{\circ}C$ below $50^{\circ}C$ Optimum reaction time 30 min. 60 min. Optimum CMC concentration 3% 3% 3. The Km values of CMCase were 0.8% and 1.01 for Aspergillus niger-SM6 and Trichoderma viride-SM10, respectively. 4. In the strain of Aspergillus niger-SM6, there were high activity of xylanase and pectinase.

  • PDF

Ursolic acid supplementation decreases markers of skeletal muscle damage during resistance training in resistance-trained men: a pilot study

  • Bang, Hyun Seok;Seo, Dae Yun;Chung, Young Min;Kim, Do Hyung;Lee, Sam-Jun;Lee, Sung Ryul;Kwak, Hyo-Bum;Kim, Tae Nyun;Kim, Min;Oh, Kyoung-Mo;Son, Young Jin;Kim, Sanghyun;Han, Jin
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.21 no.6
    • /
    • pp.651-656
    • /
    • 2017
  • Ursolic acid (UA) supplementation was previously shown to improve skeletal muscle function in resistance-trained men. This study aimed to determine, using the same experimental paradigm, whether UA also has beneficial effects on exercise-induced skeletal muscle damage markers including the levels of cortisol, B-type natriuretic peptide (BNP), myoglobin, creatine kinase (CK), creatine kinase-myocardial band (CK-MB), and lactate dehydrogenase (LDH) in resistance-trained men. Sixteen healthy participants were randomly assigned to resistance training (RT) or RT+UA groups (n=8 per group). Participants were trained according to the RT program (60~80% of 1 repetition, 6 times/week), and the UA group was additionally given UA supplementation (450 mg/day) for 8 weeks. Blood samples were obtained before and after intervention, and cortisol, BNP, myoglobin, CK, CK-MB, and LDH levels were analyzed. Subjects who underwent RT alone showed no significant change in body composition and markers of skeletal muscle damage, whereas RT+UA group showed slightly decreased body weight and body fat percentage and slightly increased lean body mass, but without statistical significance. In addition, UA supplementation significantly decreased the BNP, CK, CK-MB, and LDH levels (p<0.05). In conclusion, UA supplementation alleviates increased skeletal muscle damage markers after RT. This finding provides evidence for a potential new therapy for resistance-trained men.

Effects of Dietary Selfheal (Prunella vulgaris) Water Extracts and Its Culture Fluid with Lactobacillus rhamnosus on Growth and Immune Responses of Juvenile Olive Flounder (두메꿀풀(Prunella vulgaris) 열수추출액과 미생물(Lactobacillus rhamnosus) 배양액의 사료 내 첨가가 넙치 치어의 성장과 면역반응에 미치는 영향)

  • Kim, Sung-Sam;Galaz, German Bueno;Heo, Moon-Soo;Kim, Gi-Young;Choi, Kwang-Sik;Lee, Ki-Wan;Yeo, In-Kyu;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.40 no.5
    • /
    • pp.300-307
    • /
    • 2007
  • This study investigated the effects of dietary supplementation with hot water extracts (HE) of selfheal (Prunella vulgaris) and Lactobacillus rhamnosus culture fluid (LF) on the growth and non-specific immune responses of juvenile olive flounder. A total of 270 fish ($5.07{\pm}0.01g$, average $weight{\pm}SD$) were divided randomly into nine groups, and three groups were fed one of three isonitrogenous (51% crude protein) and isocaloric (17.6 MJ/kg) diets. The diets contained no supplement, 50 mL hot water extract, or 50 mL L. rhamnosus fluid (designated as diets CON, HE and LF, respectively). During the 8-week feeding trial, the growth, feed utilization and survival of the fish were not significantly affected by the experimental diets. There were no significant differences in the hematocrit and hemoglobin of fish fed each of the experimental diets. However, the serum alanine aminotransferase and aspartate aminotransferase activity were lower with the dietary supplements containing HE and LF. The alanine aminotransferase activity of fish fed the HE diet was significantly lower than that of fish fed the control diet. The hematocrit, hemoglobin, and nitroblue tetrazolium (NBT) activity were higher 3 hr after feeding than 24 hr afterwards. The NBT activity of fish fed the HE and LF diets were significantly higher than that of fish fed the control diet 3 hr after feeding. The findings suggest that dietary supplementation with HE and LF could enhance the nonspecific immune responses of juvenile olive flounder.

A STUDY ON CORROSION BEHAVIOR OF DENTAL AMALGAMS AS A FUNCTION OF TIME (시간경과에 따른 아말감의 부식거동에 관한 연구)

  • Lee, Myung-Jong;Kim, Yung-Hai;Lee, C.S.;Yoon, Soo-Han;Lim, Sung-Sam;Kwon, Hyuk-Choon;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
    • /
    • v.16 no.1
    • /
    • pp.6-15
    • /
    • 1991
  • The purpose of this study was to observe in vitro chloride corrosion behavior from 5 kinds of amalgam (Caulk spheracal, Amalcap, Dispersalloy, Tytin, Sybralloy) as a function of time after tritruration by using potentiostat. After each amalgam alloy and Hg was triturated as the direction of the manufacturer by the mechanical amalgamator, the triturated mass was inserted the cyrindrical matal mold ($12{\times}10mm$) and was condensed by using routine manner. The specimen was removed from the mold and was stored at room temperature for 1 week, 1 month and 3 months, and standard surface preparation was routine carried out. The 0.9% saline solution was used as electrolyte in pH 6.8~7.0 at $30{\pm}0.5.^{\circ}C$. The open circuit potential was determined after 30 minutes' immersion of 1 week, 1 month and 3 month old specimens. The scan rate was 1 mV/sec and the surface area of amalgam exposed to the solution was $0.65\;Cm^2$ for each specimen. All potentials reported are with respect to a silver / silver chloride electrode (SSE). The following result was obtained. 1. All amalgam specimens became more noble corrosion potentials which represent the improved corrosion resistance as the time elapsed. 2. Three kinds of high copper amalgam always exhibited more noble potential than low copper amalgam at 1 week, 1 month and 3 months. 3. Two kinds of low copper amalgam had the similar polarization curve pattern with 3 current peaks at each time period and current densities associated with these peaks were decreased as aging especially in caulk spherical amalgam. 4. All kinds of high copper amalgam had the similar polarization curve pattern with absence of prominent current peak at each time period, but the polarization curve of D amalgam had one apparent current peak at 1 week.

  • PDF

Lycopene Content and Fruit Morphology of Red, Pink, Orange, and Yellow Fleshed Watermelon (Citrullus lanatus) Germplasm Collections

  • Noh, Jae-Jong;Hur, On-Sook;Ro, Na-Young;Lee, Jae-Eun;Hwang, Ae-Jin;Kim, Bit-Sam;Rhee, Ju-hee;Yi, Jung Yoon;Kim, Ji Hyun;Lee, Ho-Sun;Sung, Jung-Sook;Kim, Myung-Kon;Assefa, Awraris Derbie
    • Korean Journal of Plant Resources
    • /
    • v.33 no.6
    • /
    • pp.624-637
    • /
    • 2020
  • High-quality and high-phytonutrient watermelon fruits have strong market opportunities besides their health related benefits. Hence, investigating quality and nutritional related traits of watermelon genetic resources could provide important baseline data in breeding for increased lycopene content thereby increasing the marketability of watermelon. To this end, we have examined some fruit morphological traits and lycopene content of 105 genetic resources. Seeds, originally obtained from 22+ countries, were obtained from the National Agrobiodiversity Center, Jeonju, South Korea, grown in an experimental field and harvested at a fully mature stage. The size of pistil scar (SPS), the width of stripes (WS), weight of fruit (WF), length of fruit (LF), width of fruit (WIF), the thickness of pericarp (TP), soluble solids content (SSC), fruit shape in longitudinal section, ground color of skin, the intensity of the green color of skin, fruit shape at the apical part, grooving distribution, conspicuousness of stripes, and main color of the flesh were recorded on the field and inside laboratory and the lycopene was measured using spectrophotometric and HPLC methods. Watermelon fruits have shown a diverse morphological characters. Red and pink fleshed fruits dominated in the entire collections. Fruits with higher thickness of rind were found to exhibit less soluble solid content (SSC). Korean origin fruits were characterized by intermediate SSC while the United States of America (USA), Russia (RUS), Tajikistan (TJK), Turkmenistan (TKM), Taiwan (TWN), and Uruguay (URY) originated fruits had the highest SSC. The lycopene content varied between 41.37 and 182.82 ㎍/g, 2.81 and 163.72 ㎍/g, and 3.54 and 255.47 ㎍/g using HPLC, UV-Vis spectrophotometer, and microplate reader spectrophotometer, respectively. Red- and pink-fleshed fruits had the highest levels of lycopene content compared to the yellow- and orange-fleshed. Lycopene content had a significant positive correlation with SSC, however, no correlations were detected between lycopene and other quantitative fruit morphological characters. Our study demonstrated high diversity exists in fruit morphological traits and lycopene content of the germplasm collections which provide beneficial baseline data for a future breeding program and utilization of watermelon germplasm collections in gene banks for the maintenance and improvement of the current levels of production, marketability, and health-related benefit of watermelon fruits.

Characterizations of CuInGaSe(CIGS) mixed-source and the thin film (CuInGaSe(CIGS)혼합 소스의 제작과 특성)

  • Lee, Ah-Reum;Jeon, Hun-Soo;Lee, Gang-Suok;Ok, Jin-Eun;Cho, Dong-Wan;Kim, Kyung-Hwa;Yang, Min;Yi, Sam-Nyeong;Ahn, Hyung-Soo;Cho, Chae-Ryong;Son, Sang-Ho;Ha, Henry
    • Journal of the Korean Crystal Growth and Crystal Technology
    • /
    • v.20 no.1
    • /
    • pp.1-6
    • /
    • 2010
  • CuInGaSe(CIGS) mixed-source was prepared by hydride vapor phase epitaxy (HVPE). Each metal was mixed in regular ratio and soaked at $1090^{\circ}C$ for 90 minutes in nitrogen atmosphere. After making the mixed-source to powder state, the pellet was made by the powder. The diameter of pellet is 10 mm. The CIGS thin film was deposited on soda lime glass evaporated Mo layer bye-beam evaporator. To confirm the crystallization, we measured X-ray diffraction (XRD). High intensity X-ray peaks diffracted from (112), (204)/(220), (116)/(312) and (400) of CIGS thin film and from (110) of Mo were confirmed by XRD measurement.

Case Report of 6 Patients Not Improved after Conservative Treatment of Herniated Lumbar Intervertebral Disc (요추간판탈출증으로 보존적 치료후 호전되지 않는 환자 6 례 보고)

  • Lee, Sam-Ro;Kim, Hyun-Jung;Byun, Jae-Young;Ahn, Soo-Gi;Lee, Jong-Deok
    • Journal of Acupuncture Research
    • /
    • v.17 no.3
    • /
    • pp.265-276
    • /
    • 2000
  • Objectives : The purpose of this study is to observe the clinical characteristics of 6 patients who were not improved after admitted conservative treatment in this 311 patients. Methods : We retrospectivety reviewed 311 patients of Herniated Lumbar Intervertebral Disc which undenvent admitted conservative treatment from November 1997 to June 2000. 311 patients were diagnosed by computed tomography or magnetic resonance imaging in addition to physical examination. Other complications were not detected in 311 patients with Hemiated Lumbar Intervertebral Disc. In case that the scoliosis appeared to caused by severe pain, we included this scoliosis patient with the object of survey. Results : 1. In 6 patients who were not improved, 4 patients were large herniation and 2 patients were medium herniation. 2. In all of 6 patients, the angle of straight leg raising test was low and was not imporved unril discharge. 3. X-ray findings showed straightening in all of 6 patients, disc space narrowing in 3 of 6 patients. 4. X-ray findings showed scoliosis in 3 of 4 patients who were severe pain. 5. The onset was relarively long from 30 days to 7 years. 6. The age was relatively young from 10's to 30's. 7. The adimission date was ralatively long from 32 days to 105 days. Conclusions : In 6 patients, there were observed much volume of disc herniation, low angle of straight leg raising test, young people in an age group, straightening of x-ray finding.

  • PDF

A Case Report of Meralgia Paresthetica (Meralgia Paresthetica 치료(治療) 1례에(例) 대한 증례보고)

  • Na, Gun-ho;Park, Eun-ju;Shin, Jeong-cheol;Lee, Dong-hyun;Lee, Sam-ro;Ryu, Chung-ryul;Yoon, Yeo-choong;Cho, Myung-rae
    • Journal of Acupuncture Research
    • /
    • v.22 no.1
    • /
    • pp.109-115
    • /
    • 2005
  • Objective : The purpose of this case is to report the improvement after treatment about a patient with meralgia paresthetica. Methods : We treated the patient with acupuncture therapy and Herbal medication from 11th October 2004 to 15th October 2004 by evaluating femoral function with VAS score and R.O.M of femur joint. Results : After 5 times of treatment, this patient achieved excellent outcome following the technique, showing that clinical symptom as like heating sense, edema and pain was disappeared, VAS changed from 10 to 1 and there was no limitation of ROM of femur. Conclusions : Meralgia paresthetica is a symptom complex that includes numbness, paresthesias, and pain in the anterolateral thigh, which may result from either an entrapment neuropathy or a neuroma of the lateral femoral cutaneous nerve(LFCN). Oriental medical treatment for meralgia paresthetica resulted in satisfactory results by diminishing the symptoms progressively during the five days of treatment. Differential diagnosis was based on careful physical examination. More research of meralgia paresthetica is needed.

  • PDF

V-band Self-heterodyne Wireless Transceiver using MMIC Modules

  • An, Dan;Lee, Mun-Kyo;Lee, Sang-Jin;Ko, Du-Hyun;Jin, Jin-Man;Kim, Sung-Chan;Kim, Sam-Dong;Park, Hyun-Chang;Park, Hyung-Moo;Rhee, Jin-Koo
    • JSTS:Journal of Semiconductor Technology and Science
    • /
    • v.5 no.3
    • /
    • pp.210-219
    • /
    • 2005
  • We report on a low-cost V-band wireless transceiver with no use of any local oscillator in the receiver block using a self-heterodyne architecture. V-band millimeter-wave monolithic IC (MMIC) modules were developed to demonstrate the wireless transceiver using coplanar waveguide (CPW) and GaAs PHEMT technologies. The MMIC modules such as the MMIC low noise amplifier (LNA), medium power amplifier (MPA) and the up/down-mixer were installed in the transceiver system. To interface the MMIC chips with the component modules for the transceiver system, CPW-to-waveguide fin-line transition modules of WR-15 type were designed and fabricated. The fabricated LNA modules showed a $S_{21}$ gain of 8.4 dB and a noise figure of 5.6 dB at 58 GHz. The MPA modules exhibited a gain of 6.9 dB and a $P_{1dB}$ of 5.4 dBm at 58 GHz. The conversion losses of the up-mixer and the down-mixer module were 14.3 dB at a LO power of 15 dBm, and 19.7 dB at a LO power of 0 dBm, respectively. From the measurement of V-band wireless transceiver, a conversion gain of 0.2 dB and a $P_{1dB}$ of 5.2 dBm were obtained in the transmitter block. The receiver block showed a conversion gain of 2.1 dB and a $P_{1dB}$ of -18.6 dBm. The wireless transceiver system demonstrated a successful data transfer within a distance of 5 meters.

Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo

  • Jo, Eun-Hye;Cho, Sung-Dae;Ahn, Nam-Shik;Jung, Ji-Won;Yang, Se-Ran;Park, Joon-Suk;Hwang, Jae-Woong;Lee, Sung-Hoon;Park, Jung-Ran;Kim, Sun-Jung;Park, Hyun-Kyung;Lee, Yong-Soon;Kang, Kyung-Sun
    • Korean Journal of Veterinary Research
    • /
    • v.45 no.1
    • /
    • pp.85-91
    • /
    • 2005
  • Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system. Nude mice bearing established breast tumors (with exogenous estradiol) were treated with BLC extract and BLC/HEN egg. Treatment BLC extract and BLC/HEN egg caused a 42% and 71% inhibition of tumor growth, respectively. Both agents caused a significant inhibition of volume and weight growth of estrogen independent human breast tumors established from MCF-7 cells. Our results suggested that BLC extract and BLC/HEN egg have the efficacious effect of human breast cancer not only in vitro but also in vivo.